Journal
HUMAN MOLECULAR GENETICS
Volume 30, Issue 17, Pages 1569-1578Publisher
OXFORD UNIV PRESS
DOI: 10.1093/hmg/ddab107
Keywords
-
Funding
- Ministry of Science and Technology of China [2018YFC-1003300]
- National Science Foundation China [31671495]
Ask authors/readers for more resources
CLP1, TSEN complex, and VCP are evolutionarily conserved proteins associated with neurodegenerative diseases, but this study found they are also involved in germline differentiation. The RNA kinase Cbc plays a role in regulating the transition from mitosis to meiosis, interacting with Tsen54 and TER94. Functional conservation between Cbc and mammalian CLP1 was illustrated in subcellular localization tests and Drosophila fertility assays.
CLP1, TSEN complex, and VCP are evolutionarily conserved proteins whose mutations are associated with neurodegenerative diseases. In this study, we have found that they are also involved in germline differentiation. To optimize both quantity and quality in gametes production, germ cells expand themselves through limited mitotic cycles prior to meiosis. Stemming from our previous findings on the correlation between mRNA 3'-processing and meiosis entry, here we identify that the RNA kinase Cbc, the Drosophila member of the highly conserved CLP1 family, is a component of the program regulating the transition from mitosis to meiosis. Using genetic manipulations in Drosophila testis, we demonstrate that nuclear Cbc is required to promote meiosis entry. Combining biochemical and genetic methods, we reveal that Cbc physically and/or genetically intersects with Tsen54 and TER94 (VCP ortholog) in this process. The C-terminal half of Tsen54 is both necessary and sufficient for its binding with Cbc. Further, we illustrate the functional conservation between Cbc and mammalian CLP1 in the assays of subcellular localization and Drosophila fertility. As CLP1, TSEN complex, and VCP have also been identified in neurodegenerations of animal models, a mechanism involving these factors seems to be shared in gametogenesis and neurogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available