Plant neighbours shape fungal assemblages associated with plant roots: A new understanding of niche-partitioning in plant communities

1. Understanding the assembly rules of mycorrhizal fungi is crucial, given their tremendous importance in plant nutrition and health. Differentiation in plant-associated arbuscular mycorrhizal fungi (AMF) is likely driven by a host-preference effect. Coexisting plant species may then affect a focal plant microbiota through fungal dispersal among plants, and plant preferential recruitment of AMF. Both mechanisms are likely shaped by the plant's phylogenetic and functional strategies. 2. We expected that (a) the structure of AMF assemblages associated with a focal plant depends on the identity of the neighbouring plant species; (b) this effect would be predicted by the phylogenetic and functional similarity between the focal and neighbouring plant species. These predictions were tested during the first stages of growth, by simulating the early development of plants within a community 3. Using an experimental matrix-focal plant species design testing 15 neighbouring plants from five taxonomic families, we demonstrated that the neighbouring plants provided different species pools for the focal plant, Medicago truncatula, and influenced AMF communities associated with focal plant, especially in terms of richness but not relative evenness. Medicago truncatula grown with Brassicaceae or other Poaceae species displayed respectively no or low AMF richness compared to those grown with Rosaceae and Asteraceae species. These effects were weakly dependent on the phylogenetic distance from the neighbouring plant but were predicted by the functional proximity. AMF assemblages were enriched and bore more resemblance to the neighbouring plants when the neighbouring plants were functionally dissimilar from the focal one. Functional dissimilarity was only a significant predictor when based on traits characterizing the nutrient use and uptake strategy rather than on a more integrated growing strategy of the plant. 4. Microbiota composition was shown to be dependent on the identity of the neighbouring plant, particularly on its functional below-ground niche. At the colonization stage, when the plant arrives in a community, plant mycobiota might be influenced by the spatial distribution of plants already present in the community. This work suggests a new view of the concept of niche partitioning in space for plants based on microorganism-plant interactions.

Automated summary

This study demonstrated that neighbouring plants from different taxonomic families provided different species pools for the focal plant, influenced the AMF communities associated with the focal plant, especially in terms of species richness. The effects were weakly dependent on phylogenetic distance but predicted by functional proximity, with AMF communities enriched and more similar to the neighbouring plants when functionally dissimilar from the focal plant.