Journal
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
Volume 50, Issue 3-4, Pages 653-660Publisher
SPRINGER
DOI: 10.1007/s00249-021-01529-1
Keywords
MST; TRIC; Thermophoresis; K-D; Interaction
Categories
Funding
- Novo Nordisk Foundation [NNF14CC0001]
- City of Vienna
- Austrian Ministry for Education, Science and Research
- VBCF ProTech
- COST Action project ARBRE-MOBIEU [CA15126]
- COST Action [CA15126, STMS 37745]
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NanoTemper Monolith instruments are widely used in academia and industry for measuring molecular interactions. The technology has been extensively reviewed, and some assumptions and limitations have been revised in the last decade. This letter presents the experience gathered in scientific service facilities and highlights possible pitfalls for scientists to promote knowledge and good practice in the scientific community.
NanoTemper Monolith instruments have gained enormous popularity for measuring molecular interactions both in academia and industry. The underlying technology has been extensively reviewed along with its assumptions, limitations, and applications (Scheuermann et al., Anal Biochem 496:79-93, 2016). Several assumptions about the technique such as the extent of thermal deviations generated by the infrared laser and the thermophoretic foundation of the measured signal have been revised during the last decade. We present here in this letter the experience gathered in scientific service facilities about this technique and make scientists aware of possible pitfalls with the intention to promote knowledge and good practice throughout the scientific community.
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