Journal
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 28, Issue 32, Pages 43382-43393Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-021-13735-7
Keywords
Particulate matter; NEAT1; miR-582-5p; HIF-1α Lung cancer; Cancer stem cells
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Funding
- National Key Research and Development Program of China [2018YFC1313600]
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PM2.5 exposure enhances lung bronchial epithelial cell proliferation and EMT, as well as elevates CSC-like biomarkers CD133 and CD44 levels. NEAT1 interacts with miR-582-5p, releasing HIF-1α and promoting EMT and CSC-like characteristics.
The hazards of particulate matter (PM2.5) on human respiratory health have been previously reported. However, the molecular mechanisms underlying PM2.5-induced lung carcinogenesis have rarely been studied. In the present study, we explored the effects of PM2.5 on the epithelial-mesenchymal transition (EMT) and acquisition of cancer stem cell (CSC)-like properties in lung bronchial epithelial cells. We found that exposure of PM2.5 enhanced lung bronchial epithelial cell proliferation and EMT. In addition, the expression level of CSC-like biomarkers, CD133 and CD44, was significantly elevated by PM2.5 in vitro. Nuclear paraspeckle assembly transcript 1 (NEAT1) has been reported to participate in lung cancer. Loss of NEAT1 represses the malignant transformation of BEAS-2B and HBE cells induced by PM2.5. NEAT1 interacts with microRNA (miR)-582-5p, and miR-582-5p reverses the pro-tumor effects of NEAT1 overexpression. Hypoxia-inducible factor (HIF)-1 alpha is an important transcription factor in the pathological responses to hypoxia. HIF-1 alpha was a predicted target for miR-582-5p, and a direct correlation between them was identified. Inhibitors of miR-582-5p rescued HIF-1 alpha expression, which was attenuated by a lack of NEAT1. In conclusion, PM2.5 increased NEAT1 expression, which, by binding with miR-582-5p, released HIF-1 alpha and promoted EMT and the acquisition of CSC-like characteristics.
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