Efficiency Correction Is Required for Accurate Quantitative PCR Analysis and Reporting

Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2

(2020) Jim F Huggett et al.   CLINICAL CHEMISTRY

The Ultimate qPCR Experiment: Producing Publication Quality, Reproducible Data the First Time

(2019) Sean C. Taylor et al.   TRENDS IN BIOTECHNOLOGY

Practical data handling pipeline improves performance of qPCR-based circulating miRNA measurements

(2017) Maurice W.J. de Ronde et al.   RNA

Reference genes for gene expression studies in the mouse heart

(2017) Adrián Ruiz-Villalba et al.   Scientific Reports

A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

(2017) Petr Kralik et al.   Frontiers in Microbiology

Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency

(2014) Jan M. Ruijter et al.   MICROCHIMICA ACTA

Single-Nucleotide Polymorphisms and Other Mismatches Reduce Performance of Quantitative PCR Assays

(2013) S. Lefever et al.   CLINICAL CHEMISTRY

Simple Absolute Quantification Method Correcting for Quantitative PCR Efficiency Variations for Microbial Community Samples

(2012) Robert Brankatschk et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Evaluation of qPCR curve analysis methods for reliable biomarker discovery: Bias, resolution, precision, and implications

(2012) Jan M. Ruijter et al.   METHODS

Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR

(2011) Antoon Lievens et al.   NUCLEIC ACIDS RESEARCH

Quantification Bias Caused by Plasmid DNA Conformation in Quantitative Real-Time PCR Assay

(2011) Chih-Hui Lin et al.   PLoS One

Linear Methods for Analysis and Quality Control of Relative Expression Ratios from Quantitative Real-Time Polymerase Chain Reaction Experiments

(2011) Robert B. Page et al.   TheScientificWorldJOURNAL

Differences in amplification efficiency of standard curves in quantitative real-time PCR assays and consequences for gene quantification in environmental samples

(2010) Stefanie Töwe et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Bias in the Cq value observed with hydrolysis probe based quantitative PCR can be corrected with the estimated PCR efficiency value

(2010) Jari Michael Tuomi et al.   METHODS

A decade of improvements in quantification of gene expression and internal standard selection

(2009) Olivier Thellin et al.   BIOTECHNOLOGY ADVANCES

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

(2009) S. A. Bustin et al.   CLINICAL CHEMISTRY

Predicting outcomes for children with neuroblastoma using a multigene-expression signature: a retrospective SIOPEN/COG/GPOH study

(2009) Joëlle Vermeulen et al.   LANCET ONCOLOGY

External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data

(2009) Joëlle Vermeulen et al.   NUCLEIC ACIDS RESEARCH

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data

(2009) J. M. Ruijter et al.   NUCLEIC ACIDS RESEARCH

Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example

(2008) D. Bru et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

qpcR: an R package for sigmoidal model selection in quantitative real-time polymerase chain reaction analysis

(2008) C. Ritz et al.   BIOINFORMATICS

Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry

(2008) Andrej-Nikolai Spiess et al.   BMC BIOINFORMATICS

Critical evaluation of methods used to determine amplification efficiency refutes the exponential character of real-time PCR

(2008) Robert G Rutledge et al.   BMC MOLECULAR BIOLOGY

Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology

(2008) Cindy J. Smith et al.   FEMS MICROBIOLOGY ECOLOGY

Statistical methods for efficiency adjusted real-time PCR quantification

(2007) Joshua S. Yuan et al.   Biotechnology Journal