Article
Cell Biology
Jeeho Kim, Young Jin Jeon, Sung-Chul Lim, Joohyun Ryu, Jung-Hee Lee, In-Youb Chang, Ho Jin You
Summary: Ephexin1 is highly expressed in patient tissues of colorectal cancer (CRC) and lung cancer (LC), and plays a critical role in promoting tumorigenesis through the Ras-mediated signaling pathway. Phosphorylated Ephexin1 at Ser16 and Ser18 (pSer16/18) may serve as an effective therapeutic target for CRC and LC as it interacts with oncogenic K-Ras to promote downstream MAPK signaling.
CELL DEATH & DISEASE
(2021)
Article
Physics, Multidisciplinary
Thomas J. Boeddeker, Kathryn A. Rosowski, Doris Berchtold, Leonidas Emmanouilidis, Yaning Han, Frederic H. T. Allain, Robert W. Style, Lucas Pelkmans, Eric R. Dufresne
Summary: Many membraneless organelles form liquid-like domains through phase separation in living cells. Microtubule networks are denser near stress granules, and depolymerized microtubule sub-units localize near the surface of stress granules. A thermodynamic model suggests a weak affinity of microtubule sub-units for stress granule interfaces. This study demonstrates a non-specific affinity of proteins and other objects for droplet interfaces in cells.
Article
Biochemistry & Molecular Biology
Laura Mediani, Francesco Antoniani, Veronica Galli, Jonathan Vinet, Arianna Dorotea Carra, Ilaria Bigi, Vadreenath Tripathy, Tatiana Tiago, Marco Cimino, Giuseppina Leo, Triana Amen, Daniel Kaganovich, Cristina Cereda, Orietta Pansarasa, Jessica Mandrioli, Priyanka Tripathi, Dirk Troost, Eleonora Aronica, Johannes Buchner, Anand Goswami, Jared Sterneckert, Simon Alberti, Serena Carra
Summary: Hsp90 is essential for SG dissolution by binding and stabilizing DYRK3, thus linking stress adaptation and cell growth through regulating condensate disassembly and translation restoration.
Article
Biochemistry & Molecular Biology
April Lo, Kristin Holmes, Shriya Kamlapurkar, Filip Mundt, Sitapriya Moorthi, Iris Fung, Shaunt Fereshetian, Jacqueline Watson, Steven A. Carr, Philipp Mertins, Alice H. Berger
Summary: Mutant KRAS and RIT1 were found to promote canonical RAS signaling, while overexpression of wild-type RIT1 partially phenocopied oncogenic RIT1 and KRAS, inducing epithelial-to-mesenchymal transition. The study suggests that RIT1 protein abundance plays a role in its pathogenic function, and chromosomal amplification of wild-type RIT1 in lung and other cancers may be tumorigenic.
Article
Chemistry, Multidisciplinary
Ziteng Liu, Yanglimin Ji, Wenjing Mu, Xiaodan Liu, Li Yan Huang, Tao Ding, Yan Qiao
Summary: Cooperative coacervation of porphyrin and polycation electrolyte leads to the formation of photoactive membraneless protocells via liquid-liquid phase separation, where J-aggregates are formed to facilitate energy transduction pathways for confinement of photocatalytic reactions within the protocell compartments.
CHEMICAL COMMUNICATIONS
(2022)
Article
Endocrinology & Metabolism
Yi-Hsuan Wei, Shu-Lang Liao, Sen-Hsu Wang, Chia-Chun Wang, Chang-Hao Yang
Summary: Simvastatin and ROCK inhibitor Y-27632 have been shown to inhibit the fibrosis process induced by TGF-beta, potentially through suppressing the RhoA/ROCK/ERK and p38 MAPK signaling pathways.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Cell Biology
Alexey L. Arkov
Summary: Research on the assembly mechanisms and functions of membraneless granules in cells is of fundamental and applied significance for cellular organization and the study of neurodegenerative disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Qian Wang, Chenqi Tao, Abdul Hannan, Sungtae Yoon, Xuanyu Min, John Peregrin, Xiuxia Qu, Hongge Li, Honglian Yu, Jean Zhao, Xin Zhang
Summary: The patterning of epithelial buds in lacrimal gland development is determined by the interplay between PI3K, Ras, and ERK signaling pathways. PI3K is activated by both IGF and FGF signaling, and also promotes ERK signaling through direct interaction with Ras. Inhibition of EGF signaling is essential for induction of lacrimal gland buds, and the cross-talk among FGF, IGF, and EGF signaling is mediated by PI3K, MAPK, and mTOR.
Article
Multidisciplinary Sciences
Kelsie J. Anson, Giulia A. Corbet, Amy E. Palmer
Summary: In this study, the researchers investigated how changes in intracellular zinc ion levels affect kinase signaling pathways using fluorescent biosensors and cell perturbations. The results showed that zinc ion fluctuations are not toxic and do not activate stress-dependent kinase signaling. Additionally, the study demonstrated that while zinc ions can inhibit phosphatases, ERK and Akt are primarily activated through upstream signaling pathways.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Zhenxing Zhang, Xin Li, Fan Yang, Chao Chen, Ping Liu, Yi Ren, Pengkai Sun, Zixiong Wang, Yongping You, Yi-Xin Zeng, Xinjian Li
Summary: This study demonstrates that DHHC9-mediated GLUT1 palmitoylation at Cys207 is essential for plasma membrane localization of GLUT1 and for tumor growth in glioblastoma cells. The findings suggest a potential therapeutic target for targeting GLUT1 in cancer treatment.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Zhentai Huang, Chi-Wei Chen, Raquel Buj, Naveen Kumar Tangudu, Richard S. S. Fang, Kelly E. E. Leon, Erika S. S. Dahl, Erika L. L. Varner, Eliana von Krusenstiern, Aidan R. R. Cole, Nathaniel W. W. Snyder, Katherine M. M. Aird
Summary: Inhibition of ATM increases macropinocytosis, promoting cancer cell survival under nutrient-poor conditions, and macropinocytosis is a metabolic vulnerability of cancer cells treated with ATM inhibitors.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Zhongling Zhu, Teng Jiang, Huirong Suo, Shan Xu, Cai Zhang, Guoguang Ying, Zhao Yan
Summary: The study found that metformin can enhance the anti-proliferative effect of anlotinib in NSCLC cells by activating AMPK and inhibiting mTOR, inducing apoptosis and oxidative stress, thus increasing the sensitivity of NSCLC cells to the anticancer drug.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Do Hyeon Pyun, Tae Jin Kim, Myeong Jun Kim, Soon Auck Hong, A. M. Abd El-Aty, Ji Hoon Jeong, Tae Woo Jung
Summary: KA can ameliorate hepatic steatosis by inhibiting ER stress through the AMPK/autophagy and AMPK/ORP150 pathways, showing therapeutic potential for NAFLD treatment. Treatment with KA reduces lipid accumulation, decreases lipogenic gene expression, and increases AMPK phosphorylation, ORP150 expression, and autophagy markers. KA administration in mice on a high-fat diet also improves hepatic lipid accumulation by enhancing AMPK phosphorylation, ORP150 expression, and autophagy markers in the liver.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Environmental Sciences
Kyeong Hwa Sim, Youn Ju Lee
Summary: PFHxS may induce developmental neurotoxicity and downregulate neural proteins GAP-43 and CaMKII via the NMDA receptor-mediated PKCs (alpha and delta)-ERK/AMPK pathways, resulting in memory impairment in adult mice.
Review
Microbiology
Darshika J. Udawatte, Alan L. Rothman
Summary: RIPK1 serves as a key regulator of cell death and inflammation, affecting the outcome of virus infections through activation of different signaling pathways. Viruses manipulate host immune responses by targeting RIPK1, highlighting potential directions for future research.
Article
Biochemistry & Molecular Biology
Yu-Ting Chou, Jeng-Kai Jiang, Muh-Hwa Yang, Jeng-Wei Lu, Hua-Kuo Lin, Horng-Dar Wang, Chiou-Hwa Yuh
Article
Oncology
Yu-Ting Chou, Li-Yang Chen, Shin-Lin Tsai, Hsiao-Chen Tu, Jeng-Wei Lu, Shih-Ci Ciou, Horng-Dar Wang, Chiou-Hwa Yuh
Article
Hematology
Helen M. McRae, Alexandra L. Garnham, Yifang Hu, Matthew T. Witkowski, Mark A. Corbett, Mathew P. Dixon, Rose E. May, Bilal N. Sheikh, William Chiang, Andrew J. Kueh, Tan A. Nguyen, Kevin Man, Renee Gloury, Brandon J. Aubrey, Antonia Policheni, Ladina Di Rago, Warren S. Alexander, Daniel H. D. Gray, Andreas Strasser, Edwin D. Hawkins, Stephen Wilcox, Jozef Gecz, Axel Kallies, Matthew P. McCormack, Gordon K. Smyth, Anne K. Voss, Tim Thomas
Article
Immunology
Tan A. Nguyen, Blake R. C. Smith, Kirstin D. Elgass, Sarah J. Creed, Shane Cheung, Michelle D. Tate, Gabrielle T. Belz, Ian P. Wicks, Seth L. Masters, Ken C. Pang
JOURNAL OF IMMUNOLOGY
(2019)
Article
Biology
Kuan Zhang, Erica Yao, Chuwen Lin, Yu-Ting Chou, Julia Wong, Jianying Li, Paul J. Wolters, Pao-Tien Chuang
Editorial Material
Cell Biology
Tan A. Nguyen, Jayanta Debnath
Article
Cell Biology
Tina A. Solvik, Tan A. Nguyen, Yu-Hsiu Tony Lin, Timothy Marsh, Eric J. Huang, Arun P. Wiita, Jayanta Debnath, Andrew M. Leidal
Summary: The endolysosome system is involved in autophagic degradation and secretory pathways, including the release of extracellular vesicles and particles (EVPs). This study reveals a secretory autophagy pathway that is upregulated during endolysosome inhibition and mediates the release of autophagic cargo receptors via EVPs. This secretion process is regulated by multiple ATGs and the small GTPase Rab27a.
JOURNAL OF CELL BIOLOGY
(2022)
Meeting Abstract
Oncology
Daniel L. Kerr, Wei Wu, Whitney Tamaki, Anatoly Urisman, Yu-Ting Chou, Philippe Gui, David M. Jablons, Trever G. Bivona, Collin M. Blakely
Review
Physiology
Tan A. Nguyen, Jayanta Debnath
Summary: This review discusses the noncanonical functions of the autophagy machinery in promoting secretory autophagy, as well as the regulation of secretory autophagy under various cellular stresses.
CURRENT OPINION IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yu-Chin Nieh, Yu-Ting Chou, Chao-Yung Wang, Shi-Xian Lin, Shih-Ci Ciou, Chiou-Hwa Yuh, Horng-Dar Wang
Summary: RPIA plays a crucial role in lung cancer by regulating cellular proliferation and activating autophagy, apoptosis, and cellular senescence. Suppression of RPIA leads to increased reactive oxygen species (ROS) levels, which in turn induce autophagy, apoptosis, and cellular senescence in lung cancer cells. This study sheds new light on the potential of RPIA suppression as a therapeutic strategy for lung cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Shigeki Nanjo, Wei Wu, Niki Karachaliou, Collin M. Blakely, Junji Suzuki, Yu-Ting Chou, Siraj M. Ali, D. Lucas Kerr, Victor R. Olivas, Jonathan Shue, Julia Rotow, Manasi K. Mayekar, Franziska Haderk, Nilanjana Chatterjee, Anatoly Urisman, Jia Chi Yeo, Anders J. Skanderup, Aaron C. Tan, Wai Leong Tam, Oscar Arrieta, Kazuyoshi Hosomichi, Akihiro Nishiyama, Seiji Yano, Yuriy Kirichok, Daniel S. W. Tan, Rafael Rosell, Ross A. Okimoto, Trever G. Bivona
Summary: This study investigates the impact of co-occurring genetic alterations on mutant EGFR and identifies the deficiency of RNA-binding factor RBM10 as a factor that decreases the efficacy of EGFR inhibitors in lung cancer treatment. The study reveals that RBM10 modulates tumor cell apoptosis by regulating the alternative splicing of Bcl-x and its deficiency diminishes EGFR inhibitor-mediated apoptosis. The findings suggest that co-occurring genetic alterations and splicing factor deficiency play a role in determining the sensitivity to targeted kinase inhibitor therapy.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Multidisciplinary Sciences
Tan A. Nguyen, Kathryn T. Bieging-Rolett, Tracy L. Putoczki, Ian P. Wicks, Laura D. Attardi, Ken C. Pang