Journal
CANCER CELL INTERNATIONAL
Volume 21, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12935-021-01882-3
Keywords
Osteosarcoma; MEX3A; Proliferation; Apoptosis; Cell cycle; Migration
Categories
Funding
- Natural Science Foundation of Zhejiang Province [LQ18H060004]
- Zhejiang Provincial Medicine Health Science and Technology Program [2017KY016]
- Health Aspect Project of Zhejiang Province [2021KY021]
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This study confirmed the overexpression of MEX3A in osteosarcoma tissues and demonstrated the inhibitory effects of MEX3A knockdown on osteosarcoma progression. It was further revealed that MEX3A may be involved in osteosarcoma development by regulating apoptosis-related factors.
Background Osteosarcoma is an aggressive malignant tumor which has attracted worldwide attention. MEX3A may be associated with tumors while has not yet seen its coverage on osteosarcoma. Herein, this study was to investigate the correlation between MEX3A and the progression of osteosarcoma. Methods Firstly, we determined that expression of MEX3A was significantly higher in osteosarcoma tissues than that in marginal bone by immunohistochemical staining. Additionally, MEX3A expression was downregulated by the RNAi-mediated knockdown. The functions of MEX3A knockdown on proliferation, apoptosis, cell cycle, migration was assessed by MTT assay, flow cytometry, wound-healing assay and Transwell assay, respectively. Knockdown of MEX3A resulted in suppressing cell proliferation, increasing cell apoptosis, inducing the G2 phase cell cycle arrest, and attenuating cellular migration. Furthermore, mouse xenograft model confirmed inhibitory effects of MEX3A knockdown on osteosarcoma formation. Results The preliminary exploration on the molecular mechanism of MEX3A in osteosarcoma cells showed that the induction of apoptosis needs the participation of a series of apoptosis- associated factors, such as upregulation of Caspase 3, Caspase 8 and HSP60, downregulation of HSP27 and XIAP. Conclusions In summary, these findings predicated that therapy directed at decreasing MEX3A expression is a potential osteosarcoma treatment.
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