Article
Immunology
Daniel Amoak, Nancy Osei Kye, Florence Wullo Anfaara, Yujiro Sano, Roger Antabe
Summary: Poverty and low education levels are among the main reasons for low maternal tetanus toxoid vaccination rates in Benin.
Article
Virology
Keren Kang, Lingfang Huang, Chaolin Ouyang, Jiaming Du, Bin Yang, Yaqin Chi, Shuyu He, Le Ying, Guoling Chen, Jihua Wang
Summary: A Rapid Antibody Test Kit was developed for the sensitive, accurate, and automated detection of IgM and IgG antibodies against SARS-CoV-2 in human samples, showing good performance in sensitivity, specificity, precision, and stability. The combined use of IgM and IgG as an alternative detection method for COVID-19 patients achieved high sensitivity and specificity, making it of great public health significance for the prevention and control of the pandemic.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Food Science & Technology
Runlin Shen, Tian Guan, Zhaodong Li, Ziling Hong, Boris B. Dzantiev, Anatoly V. Zherdev, Anastasios Koidis, Xiaojun Yao, Hongtao Lei
Summary: In this study, a highly sensitive antibody to hydrochlorothiazide was obtained and a rapid detection method based on time resolved fluorescence was developed. The strip assay showed consistent results with the confirmed method and could be used to identify adulterated hydrochlorothiazide in functional foods.
Article
Public, Environmental & Occupational Health
Ummay Nayeema Islam, Kanchan Kumar Sen, Wasimul Bari
Summary: Disparities in tetanus toxoid immunization coverage among women in Bangladesh were found, indicating the need for immunization campaigns to improve utilization of healthcare and immunization services among disadvantaged populations.
Article
Chemistry, Applied
Peiyuan Cai, Rongzhi Wang, Sumei Ling, Shihua Wang
Summary: A specific IgG monoclonal antibody for TA detection was developed in this study, along with an icELISA assay and a visual detection strip using Au@Pt0.4NP-McAb probe. Detection of trace TA in apple juice and tomato ketchup was consistent with UHPLC-MS/MS results, showing increased sensitivity compared to existing methods.
Article
Biochemical Research Methods
Zhen Long, Chen Wei, Robert Ross, Xi Luo, Xiao Ma, Yingzi Qi, Ruiping Chai, Jianming Cao, Min Huang, Tao Bo
Summary: This study investigated the effects of formaldehyde on industrial TTd and DTd, revealing the detoxification process and structural changes of toxoids. The results showed that TTd and DTd obtained from mild detoxification conditions had higher glycosylation and less impact on foreign proteins. Thus, it is suggested to use mild detoxification conditions when preparing TTd and DTd.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Immunology
Essie Komla, Oscar B. Torres, Rashmi Jalah, Agnieszka Sulima, Zoltan Beck, Carl R. Alving, Arthur E. Jacobson, Kenner C. Rice, Gary R. Matyas
Summary: Opioid use disorder is a serious health issue that has been increasing significantly in the past decade. Developing a conjugate vaccine to generate high affinity antibodies and neutralize opioids before they reach the brain is a complementary strategy to combat the crisis. The vaccine components include an opioid hapten conjugated to a carrier protein with adjuvants, and preexisting immunity to the carrier protein does not affect the immunogenicity and efficacy of the vaccine.
Article
Multidisciplinary Sciences
Bony Amin, Nitai Roy, Amatul Elah Meem, Ekhtear Hossain, Aktarujjaman
Summary: The study found a gradual decline in tetanus toxoid immunization coverage in Bangladesh from 2006 to 2019, with disparities between regions and socioeconomic groups. It highlights the importance of strengthening public health programs to ensure adequate immunization coverage, particularly among high-risk populations.
Article
Nutrition & Dietetics
Hong Shen, Yuping Wan, Xiaosheng Wu, Yu Zhang, Jingwen Li, Tingting Cui, Han Sun, Haifeng Cui, Kailun He, Guangpeng Hui, Xu Chen, Guoqiang Liu, Meihong Du
Summary: This study successfully synthesized two haptens using rational design and obtained anti-aldicarb monoclonal antibodies through immunization of mice. A colloidal gold immunochromatographic strip was developed, which provides a rapid and accurate method for detecting aldicarb in agricultural products and environments, with high sensitivity and low cross-reactivity.
FRONTIERS IN NUTRITION
(2022)
Article
Chemistry, Applied
Yu-Nan Lu, Yanke Shan, Huachuan Huang, Lin Zhu, Baojie Li, Shouyu Wang, Fei Liu
Summary: This work proposes a sensitive and quantitative on-site detecting solution for mycotoxins, which can be quickly completed on site through immunochromatography test strips, with high accuracy and good selectivity.
Article
Chemistry, Analytical
Imran Mahmood Khan, Sobia Niazi, Lin Yue, Yin Zhang, Imran Pasha, Muhammad Kashif Iqbal Khan, Wasim Akhtar, Ali Mohsin, Muhammad Farhan Jahangir Chughati, Zhouping Wang
Summary: Fluorescent nanomaterials, especially ultra-small gold nanoclusters (AuNCs), have attracted significant attention in the field of biological applications due to their bright fluorescence, economical synthesis, high photostability, biocompatibility, and deep tissue penetration. This review focuses on the advantages, synthesis principles, and recent progress of AuNCs in real-time monitoring devices, nanotechnology-based biosensing, and bioimaging sensors over the past three years (2019-2021). The challenges associated with the application of AuNCs are also discussed.
Article
Chemistry, Multidisciplinary
Piaopiao Chen, Yunjin Bai, Shiyuan Tang, Nian Wang, Yaqin He, Ke Huang, Jin Huang, Binwu Ying, Yu Cao
Summary: In this study, a simple aptamer-based toxoid test with fluorescence and binary visual readouts was developed. The test successfully detected tetanus toxoid in clinical serum samples and accurately monitored the levels before and after drug treatment.
Article
Chemistry, Analytical
Yimeng Geng, Sizhe Zhang, Yuxian Wang, Nengsheng Ye, Yuhong Xiang
Summary: A fluorescent switching based on BSA-stabilized gold nanoclusters and kanamycin aptamer was developed for the detection of Kana. The method achieved a good linear response for Kana in the concentration range of 0.04 nM to 7.0 nM. The assay showed satisfactory accuracy and recovery for detecting Kana in milk matrix, offering a novel assay for the detection of small molecules.
MICROCHEMICAL JOURNAL
(2021)
Article
Food Science & Technology
Jie Chen, Zhuqin Liu, Jinxin Fang, Yuanxiu Wang, Yi Cao, Wenjing Xu, Yingying Ma, Xiangyong Meng, Baojuan Wang
Summary: In this study, a novel biosensor was designed for the detection of carbaryl using flavourzyme-stabilized gold nanoclusters. The biosensor showed excellent photo stability and sensitivity, and was successfully applied in the determination of carbaryl in fruit and vegetable samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marta Prygiel, Ewa Mosiej, Karol Wdowiak, Daniel Rabczenko, Aleksandra Anna Zasada
Summary: There is a growing interest in developing new-generation purified antigen vaccines with higher safety profiles. Subunit vaccines have lower immunogenicity and induce weaker and shorter-lasting immune responses compared to whole-cell vaccines.
Article
Chemistry, Analytical
Lu-Fang Chen, Meng-Ting Lin, Hamada A. A. Noreldeen, Hua-Ping Peng, Hao-Hua Deng, Shao-Bin He, Wei Chen
Summary: This study reports the fructose oxidase-like activity of CuO nanoparticles and successfully fabricates a tandem catalysis-based fructose sensor. The sensor shows a low limit of detection and a wide linear range for fructose, making it suitable for fructose detection in real samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Hamada A. A. Noreldeen, Kai-Yuan Huang, Gang-Wei Wu, Hua-Ping Peng, Hao-Hua Deng, Wei Chen
Summary: This study developed two deep learning models based on 3D fluorescence spectra to identify VB6Ds and transfer them for quantitative analysis in a broad concentration range. The results showed that CNN outperformed DNN in accuracy and performance, opening up new avenues for qualitative and quantitative sensing of small molecules.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Haohua Deng, Kaiyuan Huang, Lingfang Xiu, Weiming Sun, Qiaofeng Yao, Xiangyu Fang, Xin Huang, Hamada A. A. Noreldeen, Huaping Peng, Jianping Xie, Wei Chen
Summary: Researchers have increased the photoluminescence quantum yield of water-soluble gold clusters at the single-cluster level by forming bis-Schiff base linkages, providing important insights for enhancing the luminescence efficiency of gold clusters.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Hamada A. A. Noreldeen, Shao-Bin He, Kai-Yuan Huang, Chen-Ting Zhu, Qing-Lin Zhou, Hua-Ping Peng, Hao-Hua Deng, Wei Chen
Summary: This study used fluorescence spectra data and machine learning to distinguish heavy metal ions, and found that the accuracy of first-order data is higher than second-order data. Linear discriminant analysis performed well in high-dimensional data analysis and could differentiate ions with different concentrations and molar ratios.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Guolin Hong, Canping Su, Mingchun Lai, Zhongnan Huang, Zhimin Weng, Yaling Chen, Haohua Deng, Wei Chen, Huaping Peng
Summary: In this study, a novel co-reactant-mediated high-performance low-potential Au nano-cluster (AuNC)-based ECL system was successfully developed for the detection of SARS-CoV-2 N protein. The ECL system exhibited higher energy efficiency and quantum yield, with a wide linear range and low detection limit. It showed good reproducibility and stability, making it suitable for the detection of actual serum samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Hamada A. A. Noreldeen, Kai-Yuan Huang, Gang-Wei Wu, Qi Zhang, Hua-Ping Peng, Hao-Hua Deng, Wei Chen
Summary: This study developed two deep learning models based on gold nanoclusters for rapid qualitative and quantitative analysis of eight bioflavonoids in serum. The optimized models showed high efficiency and stability, achieving excellent accuracies in various applications including multiplex detection and model applicability.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Qi Zhang, Sai Cheng, Zhiming Xin, Haohua Deng, Ying Wang, Qiang Li, Gangwei Wu, Wei Chen
Summary: 1,2,3,4,6-O-pentagalloylglucose protects against acute lung injury (ALI) by inhibiting inflammation and oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Qile Li, Ke Xu, Haipeng Zhang, Zengguang Huang, Chao Xu, Zhen Zhou, Huaping Peng, Linxing Shi
Summary: In this study, a novel Ru(bpy)(3)(2+)-Au-WS2 nanocomposite ECL probe was developed for the detection of carcinoembryonic antigen (CEA). The probe showed high sensitivity and selectivity due to the synergistic effect and biocompatibility of Au nanoparticles (Au NPs) and WS2 nanosheet. The ECL platform exhibited excellent anodic ECL performance, good reproducibility, stability, and anti-interference performance.
Article
Biophysics
Quan-Quan Zhuang, Jia-Lin Yang, Hui-Na Qiu, Kai-Yuan Huang, Yu Yang, Hua-Ping Peng, Hao-Hua Deng, Hui-Qiong Jiang, Wei Chen
Summary: This study presented a novel class of luminogen called 6-Aza-2-thiothymine-decorated gold nanoclusters (ATT-AuNCs) with aggregation-induced emission property, which demonstrated potent antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA). By generating reactive oxygen species (ROS) and altering the expression of multiple target proteins in MRSA cells, ATT-AuNCs effectively eliminated MRSA. Additionally, ATT-AuNCs could serve as a wound-healing agent. Therefore, ATT-AuNCs are considered robust antimicrobial aggregation-induced emission luminogens (AIEgens) capable of targeting multiple pathways to eliminate drug-resistant bacteria.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Spectroscopy
Chen -Ting Zhu, Kai -Yuan Huang, Qing-Lin Zhou, Xiang -Ping Zhang, Gang-Wei Wu, Hua-Ping Peng, Hao-Hua Deng, Wei Chen, Hamada A. A. Noreldeen
Summary: Sulfonamides are widely used but their accumulation in the human body can cause serious diseases. Therefore, a convenient, effective, and sensitive method to detect them is needed. In this study, a sensor array using BSA-AuNCs was constructed to detect five Sulfonamides, and the changes in fluorescence intensity at different excitation wavelengths were used for identification. This method shows great potential in the fields of food and drug monitoring.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Huaping Peng, Mingchun Lai, Huijing Wang, Zhimin Weng, Yu Yang, Zhongnan Huang, Weiming Sun, Juewen Liu, Wei Chen
Summary: In this study, an energy level engineering strategy was proposed to regulate the electrochemiluminescence (ECL) performance using ligand-protected gold nanoclusters (AuNCs) as luminophores and N,N-diisopropylethylamine (DIPEA) as a coreactant. The energy level matching between the AuNCs and DIPEA effectively promoted electron transfer reactions, improving excitation efficiency and reducing trigger potential. The narrow bandgap of the AuNCs further enhanced emission efficiency. The developed theory was successfully applied to design a highly stable near-infrared ECL system with low trigger potential.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Zhongnan Huang, Zhenglian Li, Zhimin Weng, Paramasivam Balasubramanian, Mingchun Lai, Wei Chen, Juewen Liu, Huaping Peng
Summary: In this study, a model system was developed to evaluate the effect of electrocatalysis (EC) on electrochemiluminescence (ECL) through kinetic analysis of electrocatalytic reactions. The results demonstrated a dual EC-mediated ECL enhancement and identified the catalytically active sites. With a better understanding of the EC mechanism, the researchers successfully used EC to regulate ECL and developed a biosensor with a record low limit of detection.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Ruiting Chen, Hejing Chen, Huaping Peng, Yanjie Zheng, Zhen Lin, Xinhua Lin
Summary: A DNA biosensor based on a multi-walled carbon nanotube array modified electrode was developed for the detection of the MDR1 gene, which is crucial in cancer clinical therapy.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)