Journal
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
Volume 114, Issue 7, Pages 933-945Publisher
SPRINGER
DOI: 10.1007/s10482-021-01567-x
Keywords
Marine bacteria; Multilocus sequence analysis (MLSA); Nitrogen-fixing bacteria; Vibrio nitrifigilis; Vibrio; Vibrionaceae
Categories
Funding
- Ministry of Science and Technology, Taiwan [102-2621-B-002-006-MY3, 105-2311-B-002-017]
Ask authors/readers for more resources
A nitrogen-fixing marine bacterium strain Vibrio nitrifigilis sp. nov. was isolated from the sediment of Taiwan, showing unique characteristics in growth requirements and genetic composition, presenting a distinct lineage within the Vibrionaceae family. The establishment of this novel species was supported by phylogenetic, genomic, and phenotypic analyses.
A nitrogen-fixing isolate of facultatively anaerobic, marine bacterium, designated strain NFV-1(T), was recovered from the lagoon sediment of Dongsha Island, Taiwan. It was a Gram-negative rod which exhibited motility with monotrichous flagellation in broth cultures. The strain required NaCl for growth and grew optimally at about 25-35 degrees C, 3% NaCl and pH 7-8. It grew aerobically and could achieve anaerobic growth by fermenting d-glucose or other carbohydrates as substrates. NH4Cl could serve as a sole nitrogen source for growth aerobically and anaerobically, whereas growth with N-2 as the sole nitrogen source was observed only under anaerobic conditions. Cellular fatty acids were predominated by C-16:1 omega 7c, C-16:0, and C-18:1 omega 7c. The major polar lipids consisted of phosphatidylethanolamine and phosphatidylserine. Strain NFV-1(T) had a DNA G + C content of 42.5 mol%, as evaluated according to the chromosomal DNA sequencing data. Analyses of sequence similarities and phylogeny based on the 16S rRNA genes, together with the housekeeping genes, gyrB, ftsZ, mreB, topA and gapA, indicated that the strain formed a distinct species-level lineage in the genus Vibrio of the family Vibrionaceae. These phylogenetic data and those from genomic and phenotypic characterisations support the establishment of a novel Vibrio species, for which the name Vibrio nitrifigilis sp. nov. (type strain NFV-1(T) = BCRC 81211(T) = JCM 33628(T)) is proposed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available