Journal
ANIMAL FEED SCIENCE AND TECHNOLOGY
Volume 275, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.anifeedsci.2020.114766
Keywords
Metabolic gene cluster; Microbial community; PacBio SMRT; Paper mulberry silage; Species diversity
Categories
Funding
- National Key R&D project 'Processing technology research and demonstration of high-quality forage silage and moulded product' - Ministry of Science and Technology, China [2017YFD0502102]
- JIRCAS Visiting Research Fellowship Program 2019-2020 of the Japan International Research Centre for Agricultural Sciences, Japan
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In this study, PacBio single-molecule, real-time sequencing was used to explore the microbial community structure and metabolic pathways during paper mulberry silage fermentation, revealing a shift from pathogenic Proteobacteria to beneficial Firmicutes, with lactic acid bacteria playing a key role in fermentation quality.
To develop a new high-protein woody forage resource for livestock to alleviate feed shortages in the tropics, we applied PacBio single-molecule, real-time (SMRT) sequencing to explore the community structure, species diversity and metabolic gene clusters of natural microorganisms associated with paper mulberry (PM) silage fermentation. High levels of microbial diversity and abundance were observed in PM raw material, and these levels decreased with the progression of silage fermentation. During woody ensiling, the dominant bacteria shifted from pathogenic Gramnegative Proteobacteria to beneficial Gram-positive Firmicutes. Lactic acid bacteria became the most dominant bacteria that affected fermentation quality in terminal silages. Global and overview maps, carbohydrate metabolism and amino-acid metabolism were the important microbial metabolic pathways that impacted the final fermentation product of silage. PacBio SMRT sequencing revealed specific microbial-related information concerning silage. PM is rich in nutrients and macro mineral contents, which are preserved well during ensiling, indicating that PM silage can serve as a new woody resource suitable for ruminants.
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