Article
Chemistry, Analytical
Minghui Shi, Ruobing Liu, Fuyuan Zhang, Bimal Chitrakar, Xianghong Wang
Summary: In this study, single-stranded DNA (ssDNA) aptamers specific to florfenicol (FF) with high binding affinity were prepared using the magnetic bead-based systematic evolution of ligands by the exponential enrichment technique (MB-SELEX). The optimized aptamer Apt-14t, with a length of 43 nt, showed a dissociation constant 7 times higher than the full-length sequence. Molecular docking simulations confirmed the potential binding sites and interactions with FF. Additionally, a colorimetric strategy for nanogold aptamers was developed, demonstrating high accuracy in detecting florfenicol in milk and eggs samples.
Article
Chemistry, Analytical
Yuanbin Guo, Ming Shi, Xiujuan Liu, Huagang Liang, Liming Gao, Zhiwei Liu, Jian Li, Danjun Yu, Kun Li
Summary: The study utilized the SELEX method to screen aptamers recognizing A549 excreta in cell culture media, resulting in the discovery of high-affinity and selective aptamers, which could potentially be used for the discovery of specific lung cancer markers.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Ashraf M. Muhammad, Ali Zari, Nouf H. Alsubhi, Maryam H. Al-Zahrani, Rana Abdullah Alghamdi, Mai M. Labib
Summary: Aptamers, nucleic acid analogs of antibodies, have been extensively used for their specific and sensitive binding to target molecules, promising as diagnostic and therapeutic tools. To reduce the time and cost of aptamer synthesis and selection, computational approaches have been introduced. In this study, a simple sequence-based method was used to design a stable aptamer with potential binding properties to the target receptor.
Review
Cell Biology
Masaki Takahashi
Summary: G-protein-coupled receptors play crucial roles in basic science and drug discovery as essential targets. The discovery and development of modulators for GPCRs offer new therapeutic options for human diseases. However, finding new modulators remains challenging.
Review
Chemistry, Analytical
Yuan Su, Longjiao Zhu, Yifan Wu, Zihong Liu, Wentao Xu
Summary: This paper summarizes the factors influencing aptamer performance during the SELEX process and provides an advanced screening strategy for aptamers against bacterial whole-cell components. The binding sites between aptamers and bacterial whole-cell components are examined, and sequence sites are analyzed from an aptamer perspective. Additionally, the application status and prospects of bacterial aptamers are briefly described.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Jinman Zhong, Jianke Ding, Lei Deng, Ying Xiang, Duoduo Liu, Yanyan Zhang, Xin Chen, Quanxin Yang
Summary: EpCAM-specific aptamers were successfully selected using Cell-SELEX technology and conjugated with GoldMag nanoparticles to create magnetic molecular probes for MRI detection of prostate cancer. The molecular probes showed high affinity to prostate cancer cells and tissues, producing distinct T2WI signal intensity reduction in MRI.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Chemistry, Multidisciplinary
Jingye Zhang, Lu Wang, Andres Jaeschke, Murat Sunbul
Summary: FLAPs have become a promising tool for visualizing RNAs in living cells by dramatic fluorescence increase upon specific binding. In this study, a color-shifting aptamer-fluorophore system was presented, allowing ratiometric RNA imaging unlike other FLAPs reported. This system was used as a light-up RNA marker to image mRNAs in the NIR region and demonstrated its utility in ratiometric analysis of target RNAs expressed at different levels in single cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Nanoscience & Nanotechnology
Xiao Chu, Xinyu Du, Longhua Yang, Ziyi Wang, Yi Zhang, Xiaonan Wang, Lijun Dai, Jiangnan Zhang, Jie Liu, Nan Zhang, Yongxing Zhao, Hongzhou Gu
Summary: Nucleic acid-based aptamers targeting TNFR1 have been identified as potential candidates for treating Rheumatoid Arthritis (RA). These aptamers, obtained through SELEX, can inhibit TNF alpha activity by binding to TNFR1. Enhanced anti-inflammatory effects were achieved using divalent aptamer constructs.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Nan Zhang, Tao Bing, Luyao Shen, Le Feng, Xiangjun Liu, Dihua Shangguan
Summary: A high-affinity DNA aptamer (HG1-9) targeting human tumor cells was developed through cell-SELEX, showing potential as a promising ligand for general cancer-targeted diagnostics and therapeutics. The aptamer was found to bind to the human transferrin receptor and cross the epithelium barrier through transferrin receptor-mediated transcytosis for targeted drug delivery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Styliana Philippou, Nikolaos P. Mastroyiannopoulos, Marios Tomazou, Anastasios Oulas, Matthew Ackers-Johnson, Roger S. Foo, George M. Spyrou, Leonidas A. Phylactou
Summary: In vivo SELEX is an advanced method that allows the development of aptamers capable of recognizing targets directly within their natural microenvironment. By modifying the methodology, aptamers can be enriched in specific cardiac cell types, holding great potential as drug delivery vehicles.
Article
Nanoscience & Nanotechnology
Ningqin Lin, Liang Wu, Xing Xu, Qiaoyi Wu, Yuzhe Wang, Haicong Shen, Yanling Song, Hongyao Wang, Zhi Zhu, Dezhi Kang, Chaoyong Yang
Summary: Gliomas, the most common primary brain tumors, have poor prognosis and survival rates despite advancements in surgical and radio-chemotherapy technologies. The cell-SELEX technology has been used to develop the S6-1b aptamer, which can distinguish molecular differences between glioma cells and human astrocytes, showing potential for diagnostic and therapeutic applications in malignant gliomas.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Deniz Yilmaz, Tugdem Muslu, Ayhan Parlar, Hasan Kurt, Meral Yuece
Summary: Nucleic acid aptamers that can bind to bacteria and inhibit bacterial-induced inflammation have been selected through the whole-cell SELEX method. The selected aptamers showed promising results in binding to LPS and inhibiting LPS-induced macrophage polarization. These aptamers have the potential to be used as diagnostic tools and therapeutic agents for bacterial infections.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Xu Wen, Xueling Yan, Xiaoling Zheng, Qiming Kou, Lulan Yang, Jiaming Tang, Xingyue Chen, Yong Xie, Tao Le
Summary: In this study, anti-NFZ aptamers were selected and identified using the magnetic bead SELEX technique, and a truncated aptamer NFZ8-1 was optimized. A graphene oxide fluorescent aptasensor was developed using this truncated aptamer, which showed high sensitivity and accuracy for detecting NFZ residue in water samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Immunology
Marina Farrel Cortes, Taniela Marli Bes, Beatriz Ribeiro Deo, Beatriz Barbosa dos Anjos, Andres Jimenez Galisteo Jr, Ester Cerdeira Sabino, Carlos Santos, Silvia Figueiredo Costa
Summary: Infections caused by multidrug-resistant A. baumannii are a global health concern. The rapid identification and treatment of this infectious agent is challenging. Nucleic acid aptamers that can interact with bacteria offer a promising solution. Cell-SELEX technology allows the selection and identification of aptamers that target molecules present in an entire bacterial cell. However, the aptamer technology still faces challenges, such as the complexity of the screening process.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Zhukang Guo, Yuan Liu, Nongyue He, Yan Deng, Lian Jin
Summary: Aptamers are oligonucleotide chains with specific binding ability to protein and other targets, and can be used to discover novel tumor markers. Further characterization of newly acquired membrane proteins is essential for their clinical use as tumor markers.
CHINESE CHEMICAL LETTERS
(2021)
Article
Biophysics
Cong Han, Wenjin Li, Qian Li, Wenping Xing, Hang Luo, Haishuo Ji, Xiaona Fang, Zhaofeng Luo, Liyun Zhang
Summary: In this study, a CRISPR/Cas12a-derived electrochemical aptasensor for fast and ultrasensitive detection of COVID-19 nucleocapsid protein (Np) was developed. The sensor exhibited high selectivity to Np and could complete a test within 30 minutes. It showed great potential in early diagnosis of COVID-19, environmental surveillance, and food security.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Xiaona Fang, Wenjing Li, Tian Gao, Qurat Ul Ain Zahra, Zhaofeng Luo, Renjun Pei
Summary: This paper reports the development of a new strategy called integrated digital PCR-fluorescence activated sorting based SELEX (IFS-SELEX) for rapid screening of aptamers against fluorescent targets. The strategy amplifies each sequence of a preliminarily enriched library using an integrated digital PCR system, followed by fluorescence-activated sorting to collect and sequence only the sequences with high binding affinity for the targets. The experimental results demonstrate that the IFS-SELEX method can serve as a universal platform for rapid, facile, and efficient aptamer selection against various fluorescent targets.
Review
Materials Science, Multidisciplinary
Qurat ul Ain Zahra, Salim Ullah, Faisal Shahzad, Bensheng Qiu, Xiaona Fang, Ayesha Ammar, Zhaofeng Luo, Shabi Abbas Zaidi
Summary: This study discusses the application of combining 2D MXenes with high affinity aptamers in innovative aptasensing approaches for food/water safety and clinical diagnosis. The research focuses on the fabrication, sensing mechanisms, and applications of MXene-based aptasensors, with an emphasis on the detection of various types of cancers, mycotoxins, and other analytes. The study also provides insights into the future development of optical aptasensing and highlights potential constraints and prospects.
PROGRESS IN MATERIALS SCIENCE
(2022)
Article
Biophysics
Qurat ul Ain Zahra, Syed Agha Hassnain Mohsan, Faisal Shahzad, Mohammad Qamar, Bensheng Qiu, Zhaofeng Luo, Shabi Abbas Zaidi
Summary: Conventional analytical biosensor devices have limitations in handling, time, size, and cost. Smartphones coupled with aptamers have enabled the development of portable and cost-effective biosensors for biomolecule detection. This review discusses achievements in smartphone-enabled aptasensors between 2017 and 2022, covering fabrication strategies and software. The report aims to assist scientists and engineers in understanding smartphone-based aptasensor fabrication and encourage future developments in affordable and portable sensing devices.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Mengdi Yu, Xiaohui Zhang, Xin Zhang, Qurat Ul Ain Zahra, Zenghui Huang, Ying Chen, Chunxia Song, Min Song, Hongjuan Jiang, Zhaofeng Luo, Ying Lu
Summary: The COVID-19 epidemic has had a significant impact on the lives of millions of people worldwide. The development of fast and sensitive detection methods is crucial in the absence of effective global vaccination programs. This study presents an electrochemical aptamer sensor for the specific detection of COVID-19, using complementary oligonucleotides as probes. The sensor demonstrated a linear relationship between N protein concentration and electrochemical current signals, with a detection limit of 1 fM. Testing on throat swabs and blood samples from volunteers showed promising results, indicating the sensor's potential for early COVID-19 detection in patients.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Yanxin Gao, Wenjin Li, Xuan Sun, Yingying Zhao, Haishuo Ji, Hang Luo, Geng Wu, Lei Wan, Liyun Zhang
Summary: This study demonstrates the efficient and stable conversion of CO2 into formate using a photocatalytic assembly. The key role of silver nanoclusters in enhancing durability and efficiency is highlighted.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Chemistry, Analytical
Cong Han, Wenping Xing, Wenjin Li, Xiaona Fang, Jian Zhao, Feng Ge, Wei Ding, Pengpeng Qu, Zhaofeng Luo, Liyun Zhang
Summary: In this study, we proposed a biomimetic clamp assay for detecting SARS-CoV2 nucleocapsid protein (Np). The assay utilizes a pair of Np aptamers connected via a T20 spacer to form a DNA biomimetic clamp (DNA-BC). The DNA-BC is anchored on the surface of a screen-printed gold electrode, resulting in a clamp-type impedimetric aptasensor. The aptasensor shows high sensitivity and specificity for Np detection, making it a potentially promising strategy for developing reliable antigen rapid diagnostic testing (Ag-RDT) products to address the current epidemic.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Wenping Xing, Qian Li, Cong Han, Dongdong Sun, Zheng Zhang, Xiaona Fang, Yu Guo, Feng Ge, Wei Ding, Zhaofeng Luo, Liyun Zhang
Summary: This study reports a new CRISPR/Cas12a-derived aptasensor that can detect the spike protein 1 (S1) of SARS-CoV-2 as well as Beta, Delta, and Omicron variants. The study also reveals the relationship between aptamer sequence and affinity for regulating the activity of CRISPR/Cas12a. The optimized 27-nucleotide sequence shows high affinity and specificity for target recognition and efficiently activates CRISPR/Cas12a activity. This research provides new insights into aptamer development and offers a different strategy for COVID-19 antigen detection and biosensor development.
Article
Chemistry, Analytical
Meng Jiang, Xiaona Fang, Han Diao, Shaokang Lv, Zheng Zhang, Xiang Zhang, Zhiwei Chen, Zhaofeng Luo
Summary: In this study, a semi-automatic instrument was constructed and optimized for performing aptamer SELEX targeting multiple proteins simultaneously. This work provides a simple SELEX platform with real-time feedback, which is time efficient and minimizes human intervention. A number of aptamers were quickly screened and their binding affinities were verified by various methods, including SPR and flow cytometry, demonstrating the applicability and reliability of the newly established aptamer SELEX system.
ANALYTICAL METHODS
(2023)
Review
Chemistry, Analytical
Qurat ul Ain Zahra, Xiaona Fang, Zhaofeng Luo, Salim Ullah, Shazia Fatima, Sadaf Batool, Bensheng Qiu, Faisal Shahzad
Summary: In view of environmental pollution, there is a need for cheap, sensitive and fast biosensing systems for monitoring pollutants. Aptamers have advantages in biosensing devices. Graphene-based nanohybrids combined with detection methods show great potential for monitoring environmental contaminants. This review discusses the design, principle, advances, and applications of graphene-based nanohybrid aptasensors for monitoring pesticides, heavy metals, bacteria, antibiotics, and organic compounds in different environmental samples.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)