Article
Engineering, Multidisciplinary
Shuai Bu, Meilin Yu, Qin Chen, Dong Chen, Pengfei Xia, Guifei Li, Kunxi Zhang, Shifeng Yan, Haimiao Wu, Jingbo Yin
Summary: Injectable microcarriers with osteogenic and angiogenic properties were fabricated for the repair of alveolar bone defects. The ICA@nCP/nHA-g-PBLG composite microcarriers showed enhanced proliferation of bone marrow mesenchymal stem cells and demonstrated promising potential for the restoration of alveolar bone defects.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Cell & Tissue Engineering
Charan Thej, Sudha Balasubramanian, Mathiyazhagan Rengasamy, Ankita Walvekar, Priyanka Swamynathan, Swathi Sundar Raj, Pradnya Shahani, Siddikuzzaman, Udaykumar Kolkundkar, Raviraja N. Seetharam, Pawan Kumar Gupta, Anish S. Majumdar
Summary: The study demonstrated the production of a second generation of pooled mesenchymal stromal cells with comparable angiogenic properties from human bone marrow samples, showing potential for treating ischemic diseases. Results showed significant repair of necrosis and improved function with intramuscular administration of these cells in an experimental model, indicating potential for limb salvage. This suggests the feasibility of manufacturing reproducible pooled allogeneic BMMSC products with robust angiogenic properties for clinical applications.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Materials Science, Biomaterials
Gilad Doron, Levi B. Wood, Robert E. Guldberg, Johnna S. Temenoff
Summary: This study investigated the impact of microcarrier type on the secretory activity of genetically modified MSCs and identified changes in MAPK pathway signaling. The results showed that culture on stiffer microcarriers promoted the secretion of IL-1Ra and in vitro angiogenic activity, while culture on both stiffer and softer microcarriers altered the secretion of other factors. Additionally, culture on softer microcarriers increased the gene expression and secretion of hepatocyte growth factor.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Mariana P. Hanga, Alvin W. Nienow, Halina Murasiewicz, Andrzej W. Pacek, Christopher J. Hewitt, Karen Coopman
Summary: The study demonstrated the use of perfluorocarbon liquid microcarriers for expanding and recovering human mesenchymal stem/stromal cells, showing that cells can successfully attach, proliferate, and maintain quality on these liquid carriers.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Haotian Qin, Jian Weng, Bo Zhou, Weifei Zhang, Guoqing Li, Yingqi Chen, Tiantian Qi, Yuanchao Zhu, Fei Yu, Hui Zeng
Summary: This study found that the concentration of 5 mM magnesium ions is the most conducive for angiogenesis and differentiation. By activating the Notch signaling pathway, magnesium ions up-regulate the expression levels of downstream genes such as Hes1 and Hes5, as well as angiogenesis-related genes including Hif-1 alpha and eNOS, thereby promoting the angiogenesis differentiation of BMSCs. Additionally, magnesium ion-induced differentiation enhances the migration and chemotaxis of BMSCs.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Masahiro Kaneko, Airi Sato, Satoru Ayano, Akio Fujita, Goro Kobayashi, Akira Ito
Summary: This study reports a novel poly(vinyl alcohol) microcarrier for the expansion of hMSCs in suspension culture, and demonstrates long-term expansion using a bead-to-bead cell transfer method. The study also presents magnetic PVA microcarriers for cell recovery.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Jeong Eun Song, Dae Hoon Lee, Gilson Khang, Sun-Jung Yoon
Summary: Collagen, with its low antigenicity and excellent cell adhesion properties, is widely used as a biomaterial for bone, cartilage, and skin regeneration. A study showed that a scaffold mixed with duck feet-derived collagen and Poly(lactic-co-glycolic acid) reduced inflammation and improved bone regeneration. By including hydroxyapatite, a key osteoconductive material, in the scaffold, we found that increasing the concentration of duck feet-derived collagen not only improved its strength, but also enhanced cell proliferation and osteogenic differentiation. Furthermore, the addition of hydroxyapatite promoted osteogenic differentiation. The study demonstrated that the 80 wt% duck feet-derived collagen/Poly(lactic-co-glycolic acid)/hydroxyapatite scaffold promotes bone mineralization and collagen deposition while reducing inflammation, making it an excellent biomaterial for bone regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Environmental
Davide Perin, Daniele Rigotti, Giulia Fredi, George Z. Papageorgiou, Dimitrios N. Bikiaris, Andrea Dorigato
Summary: This work investigates the microstructural and thermo-mechanical properties of fibers made from poly(lactic acid) (PLA)/poly(alkylene furanoate)s (PAFs) blends for textile applications. The addition of poly(dodecylene furanoate) (P12F) enhances the mechanical properties of PLA fibers, indicating immiscibility between PAFs and PLA in the blends.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Hyun Hee Jang, Youngsook Son, Gabee Park, Ki-Sook Park
Summary: This study demonstrates that bone marrow-derived mesenchymal stem cells (MSCs) have vasculogenic properties. Different populations of MSCs vary in angiogenic factor expression and vasculogenic properties. The vasculogenic MSCs (vMSCs), distinct from endothelial progenitor cells (EPCs), may contribute to neovascularization and could be a promising cellular therapy for cardiovascular diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Patricio A. Sobarzo, Andrea P. Mariman, Christian O. Sanchez, Rene A. Hauyon, Fidel E. Rodriguez-Gonzalez, Jean Medina, Ignacio A. Jessop, Gonzalo Recabarren-Gajardo, Alain Tundidor-Camba, Claudio A. Terraza
Summary: Two new silicon-containing poly(azomethine)s and poly(p-phenylvinylene)s were successfully synthesized and characterized. These materials exhibited high thermal stability and low energy transitions, laying the groundwork for potential optoelectronic applications.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Chemistry, Applied
Ziyao Gan, Yinbiao Feng, Junjiao Yang, Tianwei Tan, Jing Yang
Summary: This study investigates the copolymerization of Poly(ethylene terephthalate)-poly(ethylene oxide tere-phthalate) (PET-PEOT) with poly(lactic acid) (PLA), and the impact of two copolymerization pathways on the resulting copolymers. It was found that by copolymerizing low molecular weight PET-PEOT with PLA at gradually increasing temperatures, the resulting copolymers could retain an average PLA sequence length of up to 16. Differential scanning calorimeter (DSC) and dynamic mechanical analysis (DMA) showed that these copolymers had a random segment distribution, and the glass transition temperatures decreased with increasing PET-PEOT content. The initial decomposition temperatures of the copolymers, analyzed by thermogravimetric analysis, decreased with increasing PLA content. The mechanical properties and potential degradability of the copolymers have been significantly improved.
REACTIVE & FUNCTIONAL POLYMERS
(2022)
Article
Polymer Science
Shanshan Xu, Xing Li, Chenxuan Sun, Lingling Ni, Wenqing Xu, Wenhua Yuan, Ying Zheng, Chengtao Yu, Pengju Pan
Summary: This study examined the crystallization kinetics and crystallization-induced lamellar structure in PLA-based supramolecular polymers end-functionalized by thymine. Tuning the stereoregularity of PLA chains can lead to various crystallization and self-assembling pathways, affecting the formation and thickness of the lamellar structure. Increasing PLA block length and crystallization temperature can enhance the crystallizability of isotactic or stereocomplexed PLA chains, while suppressing the crystallizability of thymine unit.
Article
Chemistry, Applied
Qian Ren, Minghui Wu, Zhengsheng Weng, Xiuyu Zhu, Wanwan Li, Pengke Huang, Long Wang, Wenge Zheng, Masahiro Ohshima
Summary: The addition of cellulose nanofibers (CNFs) significantly reduced the half-crystallization time of the PLLA/PDLA blend, and when 3% modified CNFs were incorporated, almost exclusively SC crystallites were formed with no HC crystallite presence. This was speculated to be due to enhanced interchain molecular interactions between CNFs and PLA, presenting a feasible and efficient method for producing high-performance PLA materials with exclusively SC crystallites.
CARBOHYDRATE POLYMERS
(2022)
Article
Polymer Science
Zhaohui He, Yinbiao Feng, Cong Wang, Junjiao Yang, Tianwei Tan, Jing Yang
Summary: In this study, aliphatic poly(ethylene succinate)-based poly(ether ester)s (PES-based PEE) were synthesized and characterized. The copolymerization of PES-PDES and PLA was also investigated. The copolymers exhibited amorphous structure and compatibility, and the flexibility of the copolymers increased with increasing PES-PDES content.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hui Hu, Xiaowei Hu, Lin Li, Yan Fang, Yan Yang, Jingjing Gu, Jiadong Xu, Lisheng Chu
Summary: Exosomes derived from bone mesenchymal stem cells (BMSC-Exos) can promote angiogenesis and improve neurological functions in ischemic stroke mice via upregulation of miR-21-5p. In vitro experiments show that BMSC-Exos also enhance the functions of human umbilical vein endothelial cells (HUVECs).
Review
Cell Biology
Elia Bosch-Rue, Leire Diez-Tercero, Barbara Giordano-Kelhoffer, Luis M. Delgado, Begona M. Bosch, Mireia Hoyos-Nogues, Miguel Angel Mateos-Timoneda, Phong A. Tran, Francisco Javier Gil, Roman A. Perez
Summary: Bone is the most studied tissue in tissue regeneration field, with tissue engineering seeking to mimic successful regeneration pathways. The use of inorganic ions has therapeutic effects and lower biological risks, with biomaterials playing a key role in controlling their release.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Materials Science, Biomaterials
Barbara Blanco-Fernandez, Irene Cano-Torres, Cristina Garrido, Gerard Rubi-Sans, Lourdes Sanchez-Cid, Marta Guerra-Rebollo, Nuria Rubio, Jeronimo Blanco, Soledad Perez-Amodio, Miguel A. Mateos-Timoneda, Elisabeth Engel
Summary: The combination of TK-hAMSCs and GCV in MTs shows potential as a cell reservoir for bystander therapy, with promising results in a rodent model of human prostate cancer.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Engineering, Biomedical
Adrian Lopez-Canosa, Soledad Perez-Amodio, Eduardo Yanac-Huertas, Jesus Ordono, Romen Rodriguez-Trujillo, Josep Samitier, Oscar Castano, Elisabeth Engel
Summary: The creation of cardiac tissue models for preclinical testing remains a challenge due to the limitations in replicating the complexity of cardiac tissue in vitro. In this study, a microfluidic platform was developed to provide signaling cues to immature cardiac cells, guiding them towards an adult phenotype. The platform demonstrated control over the anisotropy of cardiac tissue and induced the upregulation of key genes through electrical stimulation, validating its potential as a powerful tool for tissue engineering.
Article
Biotechnology & Applied Microbiology
Oscar Castano, Ana Lopez-Mengual, Diego Reginensi, Andreu Matamoros-Angles, Elisabeth Engel, Jose Antonio del Rio
Summary: After spinal cord injury, transplantation of olfactory ensheathing cells (OECs) has shown potential in promoting functional improvement. Studies have found that the migratory properties of OECs are influenced by inhibitory molecules and chemical concentration differences. Research comparing the behavior of TEG3 cells seeded on functionalized nanoscale meshes of Poly(l/dl-lactic acid; PLA) nanofibers showed that 950 nm diameter PLA nanofibers had the best results in terms of cell adhesion and migration. Additionally, functionalized nanofibers with a chemical concentration increment of SDF-1 alpha/CXCL12 greatly enhanced the migratory characteristics of TEG3 cells over inhibitory substrates.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Gerard Rubi-Sans, Agata Nyga, Elena Rebollo, Soledad Perez-Amodio, Jorge Otero, Daniel Navajas, Miguel A. Mateos-Timoneda, Elisabeth Engel
Summary: The study introduced a novel method to produce mesenchymal cell-derived matrices (CDMs) aimed at mimicking the fibrotic tumor microenvironment, showing that they closely mimic the composition, structure, and mechanical properties of human fibrotic ECM. CDMs have highly reproducible composition and tunable mechanical properties, and exhibit increased doxorubicin resistance in cancer cells following chemotherapeutic treatment in comparison to 3D culture in collagen hydrogels.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Editorial Material
Pharmacology & Pharmacy
Guillermo Blanco-Fernandez, Barbara Blanco-Fernandez, Anxo Fernandez-Ferreiro, Francisco Otero-Espinar
Summary: Liquid crystals have found applications in biomedicine, particularly in therapeutics delivery, imaging, and tissue engineering, with a focus on lipid lyotropic LCs. The scientific community is encouraged to further explore the design of more complex LCs.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Barbara Blanco-Fernandez, Sergi Rey-Vinolas, Gulsun Bagci, Gerard Rubi-Sans, Jorge Otero, Daniel Navajas, Soledad Perez-Amodio, Elisabeth Engel
Summary: Breast tissue-derived matrices can serve as important biomaterials to replicate the complexity of the tumor extracellular matrix (ECM), with the addition of collagen type I improving the fidelity to breast tumors. These novel bioinks have great potential in bioprinting breast cancer models.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Materials Science, Biomaterials
Maria Godoy-Gallardo, Maria Merino-Gomez, Luisamaria C. Matiz, Miguel A. Mateos-Timoneda, F. Javier Gil, Roman A. Perez
Summary: Supramolecular hydrogels based on nucleosides have attracted great interest in tissue scaffolding, diagnostics, and drug delivery due to their biocompatibility and stimuli-responsive properties. Recent advances have improved their stability, printability, functionality, and bioactivity by using multiple gelators, incorporating different cations, and using additives such as boric acid or biomolecules. These improvements enable their use as bioinks for 3D printing and have potential applications in tissue engineering.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Nanoscience & Nanotechnology
Maria Godoy-Gallardo, Maria Merino-Gomez, Miguel A. Mateos-Timoneda, Ulrich Eckhard, F. Javier Gil, Roman A. Perez
Summary: Soft tissue defects often require biomaterials for tissue formation and vascularization. Supramolecular hydrogels, particularly guanosine-based hydrogels, have shown potential for their 3D structure and ability to sustain living cells. However, their suitability for 3D printing has been limited. This study developed a binary cell-laden hydrogel made of guanosine and guanosine 5'-monophosphate, optimized it for printing, and coated it with hyperbranched polyethylenimine for stability. The resulting scaffold showed excellent G-quadruplex formation, good printing qualities, and the ability to allow nutrient diffusion. Cell distribution, survival, and functionality were also successful. These hydrogels have the potential to improve tissue reconstruction outcomes through customized 3D bioprinting.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biotechnology & Applied Microbiology
Maria Merino-Gomez, Maria Godoy-Gallardo, Mathias Wendner, Miguel A. Mateos-Timoneda, F. Javier Gil, Roman A. Perez
Summary: Tissue defects often require grafts or transplants, but lack of available tissue can be a problem. Hydrogels have potential as tissue graft replacements due to their ability to support cell survival and encapsulate biomolecules. Guanosine-based hydrogels have been explored, but stability issues limit their use. Borate ester chemistry and boric acid derivatives were investigated to improve the stability and properties of guanosine-based hydrogels.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Maximilian Jergitsch, Zoe Allu Allue-Mengual, Roman A. Perez, Miguel A. Mateos-Timoneda
Summary: This study explores the effect of different salt, sucrose, and MC concentrations on the printability of methylcellulose hydrogels. Increasing G' improves shape fidelity, while lowering tan(delta) improves shape fidelity as well. Lowering MC concentration, extrusion rate, and nozzle gauge can increase cell viability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Meeting Abstract
Cell & Tissue Engineering
Sergi Rey-Vinolas, Soledad Perez-Amodio, Miguel A. Mateos-Timoneda, Elisabeth Engel
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Gerard Rubi-Sans, Agata Nyga, Jordi Camps, Soledad Perez-Amodio, Miguel A. Mateos-Timoneda, Elisabeth Engel, Barbara Blanco-Fernandez
TISSUE ENGINEERING PART A
(2022)
Article
Polymer Science
Aitor Tejo-Otero, Felip Fenollosa-Artes, Isabel Achaerandio, Sergi Rey-Vinolas, Irene Buj-Corral, Miguel angel Mateos-Timoneda, Elisabeth Engel
Summary: It is difficult to mimic the mechanical properties of soft living tissues with currently available materials and technologies. The lack of information about the mechanical properties of these tissues is also a significant problem. The use of phantoms offers a promising solution to simulate biological bodies. This study tested a wide range of organs and hydrogels regarding their mechanical properties and found significant differences among the results for different soft tissues.