Journal
REGENERATIVE THERAPY
Volume 16, Issue -, Pages 53-62Publisher
ELSEVIER
DOI: 10.1016/j.reth.2021.01.001
Keywords
Extrusion-based 3D printing; Tissue regeneration; Osteogenic differentiation; Icariin; Porous scaffolds; Controlled release
Categories
Funding
- National Key R&D Program of China [2018YFB1105600]
- National Natural Science Foundation of China (NSFC) [81971734, U1605225, 31800794]
- Program for Innovative Research Team in Science and Technology in Fujian Province University
Ask authors/readers for more resources
This study fabricated icariin-encapsulated polymeric scaffolds using low-temperature extrusion-based 3D printing technology to efficiently promote osteogenesis. The composite scaffolds maintained the bio-efficacy of icariin and were characterized before and after printing. These scaffolds exhibited good biodegradability, biocompatibility, sustained release of icariin, and promoted the proliferation and differentiation of mouse bone marrow mesenchymal stem cells efficiently.
Despite the accessibility to porous architectures through various biofabrication approaches for tissue engineering, incorporating various active growth regulators within their matrices that act as biochemical cues is also an essential attribute for effective tissue growth. To address these facts, icariin (ICA)encapsulated polymeric scaffolds are fabricated using a low-temperature extrusion-based threedimensional (3D) printing technology for efficiently promoting osteogenesis. This approach not only resulted in the generation of porous architectures but also substantially maintained the bio-efficacy of the encapsulated ICA. Moreover, these composite scaffolds based on poly(epsilon-caprolactone) (PCL) and tricalcium phosphate (b-TCP) encapsulated with ICA (ITP scaffolds) are systematically characterized using various techniques before and after printing. Furthermore, various investigations relevant to biodegradability, biocompatibility, ICA release, and osteogenic ability of the ITP scaffolds are explored. The intact physiochemical properties of the materials, sustained release of ICA from the scaffolds, and high biosafety at various levels ranging from cellular to animal efficiently promoted the proliferation of mouse bone marrow mesenchymal stem cells (BMSCs) and their differentiation to osteoblasts. Together, the utilization of low-temperature extrusion approach provides a convenient and eco-friendly means of fabricating highly porous 3D architectures that supply the required growth regulators in their active form for tissue regeneration. (c) 2021, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available