Article
Environmental Sciences
Paolo Cocci, Gilberto Mosconi, Francesco Alessandro Palermo
Summary: Tributyltin (TBT) induces imposex in marine snails through the retinoid X receptor (RXR)-dependent pathway, with potential effects on processes such as differentiation, apoptosis, and embryonic development. This study investigated the RXR expression changes in Tritia mutabilis embryos exposed to different TBT concentrations, showing time-dependent alterations in gene transcription and DNA methylation levels. Overall, these findings suggest that RXR plays a crucial role in early T. mutabilis embryo development and may mediate epigenetic changes induced by TBT exposure.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Pulak R. Manna, Arubala P. Reddy, Jangampalli Adi Pradeepkiran, Sudhir Kshirsagar, P. Hemachandra Reddy
Summary: Retinoids enhance the expression and biosynthesis of StAR and pregnenolone in hippocampal neuronal cells. The retinoic acid receptor (RAR)/retinoid X receptor (RXR)-liver X receptor (LXR) sequence motif plays an important role in retinoid responsiveness. StAR protects against toxicities induced by mutant APP or mutant Tau and modulates neurosteroidogenesis and hormonal balance, suggesting its potential role in protecting against Alzheimer's disease and age-related complications and diseases.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Review
Biochemical Research Methods
Pierre Germain, Natacha Rochel, William Bourguet
Summary: Retinoids, including vitamin A and its metabolites, play critical roles in regulating essential biological processes by controlling gene expression through binding to nuclear transcription factors. Heterodimers formed by retinoic acid receptors and retinoid X receptors mediate the pleiotropic effects of retinoids by recruiting co-regulatory complexes to target gene promoters.
EXPLORING NUCLEAR RECEPTORS
(2021)
Review
Environmental Sciences
Przemyslaw Nizinski, Anna Blazewicz, Joanna Konczyk, Rajmund Michalski
Summary: Research has shown significant associations between perchlorate exposure and human health, leading to in-depth discussions on its sources, properties, determination methods, and impacts on human health. Future studies should focus on further exploring the effects of perchlorate exposure on human health.
REVIEWS ON ENVIRONMENTAL HEALTH
(2021)
Article
Chemistry, Medicinal
Yuta Takamura, Izumi Kato, Manami Fujita-Takahashi, Midori Azuma-Nishii, Masaki Watanabe, Rui Nozaki, Masaru Akehi, Takanori Sasaki, Hiroyuki Hirano, Hiroki Kakuta
Summary: This study conducted a detailed assessment of fetal transferability and teratogenicity of Bexarotene in rats. The results showed that repeated administration of Bexarotene during pregnancy caused significant fetal atrophy and bone dysplasia. Additionally, Bexarotene was found to accumulate in the placenta.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Juliette Legler, Daniel Zalko, Fabien Jourdan, Miriam Jacobs, Bernard Fromenty, Patrick Balaguer, William Bourguet, Vesna Munic Kos, Angel Nadal, Claire Beausoleil, Susana Cristobal, Sylvie Remy, Sibylle Ermler, Luigi Margiotta-Casaluci, Julian L. Griffin, Bruce Blumberg, Christophe Chesne, Sebastian Hoffmann, Patrik L. Andersson, Jorke H. Kamstra
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Food Science & Technology
Ondrej Brozman, Barbara Kubickova, Pavel Babica, Petra Laboha
Article
Toxicology
Miriam N. Jacobs, Annamaria Colacci, Raffaella Corvi, Monica Vaccari, M. Cecilia Aguila, Marco Corvaro, Nathalie Delrue, Daniel Desaulniers, Norman Ertych, Abigail Jacobs, Mirjam Luijten, Federica Madia, Akiyoshi Nishikawa, Kumiko Ogawa, Kiyomi Ohmori, Martin Paparella, Anoop Kumar Sharma, Paule Vasseur
ARCHIVES OF TOXICOLOGY
(2020)
Review
Medicine, Legal
Mirjam Luijten, Raffaella Corvi, Jyotigna Mehta, Marco Corvaro, Nathalie Delrue, Susan Felter, Bodo Haas, Nicola J. Hewitt, Gina Hilton, Thomas Holmes, Miriam N. Jacobs, Abigail Jacobs, Franz Lamplmair, Dick Lewis, Federica Madia, Irene Manou, Stephanie Melching-Kollmuss, Frederic Schorsch, Katrin Schutte, Fiona Sewell, Christian Strupp, Jan Willem van der Laan, Douglas C. Wolf, Gerrit Wolterink, Ruud Woutersen, Zvonimir Zvonar, Harm Heusinkveld, Hedwig Braakhuis
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Barbara Kubickova, Joerg A. Schenk, Franziska Ramm, Kornelija Markuskiene, Jochen Reetz, Paul Dremsek, Paulius Lukas Tamosiunas, Laima Cepulyte, Hoai Anh Trinh, Johannes Scholz, Henry Memczak, Marc Hovestaedt, Rene Ryll, Rasa Petraityte-Burneikiene, Victor M. Corman, Anika Andersson, Dietmar Becher, Martin H. Groschup, Stefan Kubick, Frank Sellrie, Reimar Johne, Rainer G. Ulrich
Summary: A hepatitis E virus (HEV) genotype 3-specific monoclonal antibody (mAb) was successfully generated by immunising BALB/c mice with a carboxy-terminal part of its capsid protein expressed in Escherichia coli. The mAb, named G117-AA4, showed strong reactivity with homologous and heterologous HEV-3 capsid proteins, as well as with capsid protein derivatives from other genotypes and related viruses. This novel mAb may prove valuable for further studies on HEV infections and diagnostic purposes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Yooheon Park, Da-Woon Jung, Anne Milcamps, Masahiro Takeyoshi, Miriam N. Jacobs, Keith A. Houck, Atsushi Ono, Toine F. H. Bovee, Patience Browne, Nathalie Delrue, YunSook Kang, Hee-Seok Lee
Summary: This study introduced and validated an androgen receptor (AR) transactivation assay for the detection of AR agonists and antagonists using a stably transfected human prostate cancer cell line. The assay showed high concordance in chemical classifications and stability across different laboratories, with accurately predicted results.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Environmental Sciences
Ioanna Katsiadaki, Tim Ellis, Linda Andersen, Philipp Antczak, Ellen Blaker, Natalie Burden, Tom Fisher, Christopher Green, Briony Labram, Audrey Pearson, Karina Petersen, Dan Pickford, Chris Ramsden, Anita Ronneseth, Kathy Ryder, Dominic Sacker, Chloe Stevens, Haruna Watanabe, Hiroshi Yamamoto, Fiona Sewell, Penny Hawkins, Hans Rufli, Richard D. Handy, Samuel K. Maynard, Miriam N. Jacobs
Summary: The fish acute toxicity test (TG203; OECD, 2019) is widely used in global hazard and risk assessment, with potential refinements such as reducing fish numbers and shortening test duration to minimize fish suffering. Standardisation of clinical sign data recording, training, and harmonisation of reporting templates are essential to improve data quality.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Review
Biochemistry & Molecular Biology
Daniel Desaulniers, Paule Vasseur, Abigail Jacobs, M. Cecilia Aguila, Norman Ertych, Miriam N. Jacobs
Summary: Epigenetics plays a crucial role in cellular functions and differentiation through mechanisms involving histone and DNA modifications as well as non-coding RNAs. Both epigenetic anomalies and DNA mutations contribute to cellular dysfunctions, including carcinogenesis, in cancers regardless of the initial carcinogenic events' nature. As part of non-genotoxic mechanisms of action, epigenetic assays integrated into testing frameworks can aid in identifying chemical carcinogens and improving public health protection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Gelsomina Pillo, Maria Grazia Mascolo, Cristina Zanzi, Francesca Rotondo, Stefania Serra, Francesco Bortone, Sandro Grilli, Monica Vaccari, Miriam N. Jacobs, Annamaria Colacci
Summary: In this study, the Transformics Assay was used to test the lowest transforming concentration of the carcinogenic chemical B(a)P in order to identify molecular signatures and mechanisms of chemical-induced transformation. The results showed that the activation of AhR led to a cascade of cellular events and modifications in the microenvironment, with the immune and inflammatory responses playing a major role in cell response. Pathways and processes related to cell cycle regulation, cytoskeletal adhesion and remodeling, cell differentiation and transformation were also observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Legal
Carl Westmoreland, Hans J. Bender, John E. Doe, Miriam N. Jacobs, George E. N. Kass, Federica Madia, Catherine Mahony, Irene Manou, Gavin Maxwell, Pilar Prieto, Rob Roggeband, Tomasz Sobanski, Katrin Schutte, Andrew P. Worth, Zvonimir Zvonar, Mark T. D. Cronin
Summary: New Approach Methodologies (NAMs) include in vitro, in silico, or chemistry-based methods that can provide information for chemical safety assessment. The European Union's current chemical legislation has limitations on the widespread use of NAMs, although some are considered suitable for regulatory purposes. A workshop identified key points to increase the use and regulatory acceptance of NAMs, such as changes in regulatory frameworks, education, training, stakeholder engagement, and addressing gaps in the scientific basis of NAMs.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2022)
Article
Food Science & Technology
Sabine Ziesemer, Susann Meyer, Julia Edelmann, Janita Vennmann, Celine Gudra, Denise Arndt, Marcus Effenberg, Olla Hayas, Aref Hayas, Johanna Sophia Thomassen, Barbara Kubickova, Dierk-Christoph Poether, Jan-Peter Hildebrandt
Summary: Cylindrospermopsin (CYN) is a cyanobacterial toxin that has delayed effects on animals and humans. This study investigated the effects of CYN on protein expression in human bronchial epithelial cells using a proteomic approach. The results showed that CYN affected the expression levels of proteins involved in various cellular functions, including protein stability regulation, cell adhesion and integration, cell proliferation, cell cycle regulation, and cytokinesis. In addition, CYN reduced cell adhesion, attenuated cell proliferation, delayed mitosis completion, and induced genomic instability.
Review
Biochemistry & Molecular Biology
Annamaria Colacci, Raffaella Corvi, Kyomi Ohmori, Martin Paparella, Stefania Serra, Iris Da Rocha Carrico, Paule Vasseur, Miriam Naomi Jacobs
Summary: This article describes the history of the development of cell transformation assays (CTAs), providing an overview from their origin to the new transcriptomic-based CTAs. The application of this knowledge is used to address how different types of CTAs can be included in the integrated approach to testing and assessment (IATA) for non-genotoxic carcinogens. The article also discusses how different CTA models fit into the preceding steps of the IATA, and maps complementary key biomarkers to represent the complexity of (non-genotoxic) carcinogenesis process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Legal
D. Basketter, S. Beken, H. Bender, J. Bridges, S. Casati, M. Corvaro, S. Cuvellier, B. Hubesch, A. Irizar, M. N. Jacobs, P. Kern, F. Lamplmair, I Manou, B. P. Mueller, R. Roggeband, L. H. Rossi
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2020)