Article
Biotechnology & Applied Microbiology
Ivonne Gonzalez-Gamboa, Edith Velazquez-Lam, Matias Jose Lobo-Zegers, Ada Itzel Frias-Sanchez, Jorge Alfonso Tavares-Negrete, Andrea Monroy-Borrego, Jorge Luis Menchaca-Arrendondo, Laura Williams, Pablo Lunello, Fernando Ponz, Mario Moises Alvarez, Grissel Trujillo-de Santiago
Summary: TuMV, a plant viral nanoparticle, shows promise as an additive for GelMA hydrogels in engineering mammalian tissues. Its nanomesh structure can serve as a nanoscaffold for cells. Covalently conjugating TuMV particles with EGF increases cell confluency on the functionalized scaffolds.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mohammad B. Aljaber, Fiona Verisqa, Zalike Keskin-Erdogan, Kapil D. Patel, David Y. S. Chau, Jonathan C. Knowles
Summary: Approximately half of an adult human's body weight is made up of muscles. The source and bloom number of gelatin have been found to have an impact on the mechanical properties and biological activities of GelMA hydrogels.
Article
Engineering, Biomedical
Cigdem Bilici, Asena G. Tatar, Efsun Senturk, Caner Dikyol, Bahattin Koc
Summary: Recent studies have shown promising outcomes in 3D bioprinting of GelMA hydrogels with cell-laden structures for tissue engineering applications. However, the reliance on photo-induced gelation processes and the lack of alternative crosslinking methods have posed challenges. This study presents a novel approach using embedded bioprinting and bisulfite-initiated radical polymerization to form cell-laden GelMA hydrogel constructs, resulting in printed structures with high cell viability.
Article
Biochemistry & Molecular Biology
Ryoma Goto, Eisaku Nishida, Shuichiro Kobayashi, Makoto Aino, Tasuku Ohno, Yuki Iwamura, Takeshi Kikuchi, Jun-ichiro Hayashi, Genta Yamamoto, Masaki Asakura, Akio Mitani
Summary: The study found that visible-light-crosslinked GelMA hydrogels, along with encapsulated osteoblasts, exhibited good mechanical and biological properties, making them suitable for use as scaffolds for bone regeneration. Osteoblasts encapsulated in GelMA hydrogels photopolymerized with visible light showed higher cell viability. Additionally, GelMA-RF hydrogels promoted osteoblast differentiation and have the potential to treat bone-destructive diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
David Grijalva Garces, Carsten Philipp Radtke, Juergen Hubbuch
Summary: A novel strategy was developed to process gelatin at room temperature using urea, which enabled the production of gelatin-methacryloyl derivatives (GelMA). The degree of functionalization of GelMA was controlled by the ratio of reactant volume to gelatin concentration. Hydrogels with tailored mechanical properties were produced by varying GelMA concentration and degree of functionalization. The biocompatibility of the hydrogels formulated with GelMA produced by this method was comparable to those produced by the conventional method.
Review
Engineering, Biomedical
Mahmoud A. Sakr, Kabilan Sakthivel, Towsif Hossain, Su Ryon Shin, Sumi Siddiqua, Jaehwan Kim, Keekyoung Kim
Summary: Gelatin methacryloyl (GelMA) is a commonly used hydrogel in tissue engineering and drug delivery due to its excellent functionality and versatile mechanical properties. However, its weak mechanical strength can be improved by blending it with other natural and synthetic biomaterials to enhance its physical properties.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Biochemistry & Molecular Biology
Hitendra Kumar, Kabilan Sakthivel, Mohamed G. A. Mohamed, Emilie Boras, Su Ryon Shin, Keekyoung Kim
Summary: Optimized synthesis parameters were used to develop GelMA bioinks with slow sol-gel transition at room temperature and visible light crosslinkable functions. Eight GelMA combinations suitable for DLP-SLA bioprinting were identified through systematic physical and rheological property characterizations. RO-GelMA bioinks were found to be most suitable for achieving high resolution in DLP-SLA printing, showing excellent biocompatibility and cell survival rates.
MACROMOLECULAR BIOSCIENCE
(2021)
Review
Biotechnology & Applied Microbiology
Yang-Hee Kim, Jonathan Dawson, Richard O. C. Oreffo, Yasuhiko Tabata, Dhiraj Kumar, Conrado Aparicio, Isha Mutreja
Summary: Musculoskeletal disorders pose a significant burden on the global economy and public health. Hydrogels, especially Gelatin methacryloyl (GelMA) hydrogel, hold great potential for enhancing the repair of damaged musculoskeletal tissues. This review provides a comprehensive overview of GelMA synthesis, hydrogel design, and its applications in regenerative medicine, particularly in the field of musculoskeletal drug delivery.
BIOENGINEERING-BASEL
(2022)
Article
Engineering, Manufacturing
Frederik Wulle, Oliver Gorke, Sarah Schmidt, Maximilian Nistler, Gunter E. Tover, Oliver Riedel, Alexanser Verl, Achim Weber, Alexanser Southan
Summary: This paper introduces the potential and advantages of multi-axis 3D printing of hydrogels, and demonstrates through experiments the relative flexibility and water absorbency of 3D printed hydrogels compared to conventional molded hydrogels. The research also highlights related research questions, such as cross-linking chemistry and formulation of hydrogel inks.
ADDITIVE MANUFACTURING
(2022)
Article
Biotechnology & Applied Microbiology
Taotao Liu, Yuzhuo Zhang, Mingyue Sun, Meiqi Jin, Wei Xia, Huazhe Yang, Tianlin Wang
Summary: This study investigated the effects of freezing conditions on GelMA hydrogels, finding that lower freezing temperatures and longer freezing times led to smaller pore sizes and enhanced cell viability and proliferation. The tunable microstructure of GelMA hydrogels can be achieved by regulating freezing conditions, offering promising prospects for tissue engineering applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Polymer Science
Ines Machado, Catarina F. Marques, Eva Martins, Ana L. Alves, Rui L. Reis, Tiago H. Silva
Summary: Marine-origin gelatin, a safe alternative to mammalian-origin materials, has the benefits of structural similarity and low production cost. Gelatin-methacryloyl (GelMA)-based hydrogels have shown excellent capacity for biomedical applications, such as tissue engineering. In this study, fish gelatin was extracted from Greenland halibut skins and functionalized with methacrylation to develop a photosensitive GelMA. By combining GelMA with glycosaminoglycans (GAGs), biomechanically stable hydrogels with tunable physicochemical features were produced. These hybrid hydrogels, tested as a matrix for chondrocyte culture, showed improved cell viability and have potential for cartilage tissue engineering applications.
Review
Biochemistry & Molecular Biology
Yuyue Zhang, Hong Chen, Jianshu Li
Summary: Hydrogels with high water content and porous structures are ideal 3D scaffolds for tissue engineering. Gelatin methacryloyl (GelMA) hydrogels, which contain cell responsive peptide sequences, have been widely used due to their adjustable mechanical properties, good processing performance, and excellent biocompatibility. This review discusses recent advancements in the microstructures of GelMA-based hydrogels, including their preparation methods, unique characteristics, and specific applications in cell culture and tissue engineering. The precise control of GelMA-based hydrogel microstructures offers great potential for universal scaffolds in tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Dermatology
Hongyu Wang, Jiaming Wan, Zhiqiang Zhang, Ruixing Hou
Summary: Advancements in GelMA hydrogels are revolutionizing regenerative medicine and tissue engineering, with its potential applications in wound healing and skin regeneration. Challenges remain in scaling up 3D bioprinting techniques and developing advanced bioinks.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Yubingqing Qi, Ting Zou, Waruna Lakmal Dissanayaka, Hai Ming Wong, Luiz E. Bertassoni, Chengfei Zhang
Summary: This study aimed to explore methods to enhance angiogenesis in the root canal by fabricating built-in microchannels with different tapers. Through experiments with varying ratios of HUVECs-GFP and SCAPs implanted in GelMA hydrogel of different concentrations, it was found that coculturing SCAPs and HUVECs-GFP at a 1:1 ratio in 0.04 taper fluidic microchannels fabricated with 5% GelMA hydrogel was the optimal condition to promote angiogenesis.
JOURNAL OF ENDODONTICS
(2021)
Article
Engineering, Biomedical
Changjiang Liu, Qifan Yu, Zhangqin Yuan, Qianping Guo, Xiting Liao, Feng Han, Tao Feng, Guoping Liu, Runze Zhao, Zhuang Zhu, Haijiao Mao, Caihong Zhu, Bin Li
Summary: This study proposes a strategy to construct viscoelastic GelMA-based hydrogels with controllable stiffness and energy dissipation using a small molecule, 3,4-dihydroxybenzaldehyde (DB), as fast dynamic bridges. These hydrogels exhibit self-healing property, injectability, excellent adhesion, and mechanical properties, and promote the spreading, proliferation, osteogenesis, and chondrogenesis of BMSCs. The viscoelastic GelMA hydrogels show the best effect in cartilage and subchondral bone repair.
BIOACTIVE MATERIALS
(2023)
Article
Oncology
Thomas Kryza, Nathalie Bock, Scott Lovell, Anja Rockstroh, Melanie L. Lehman, Adam Lesner, Janaththani Panchadsaram, Lakmali Munasinghage Silva, Srilakshmi Srinivasan, Cameron E. Snell, Elizabeth D. Williams, Ladan Fazli, Martin Gleave, Jyotsna Batra, Colleen Nelson, Edward W. Tate, Jonathan Harris, John D. Hooper, Judith A. Clements
MOLECULAR ONCOLOGY
(2020)
Article
Polymer Science
Margaux Vigata, Christoph Meinert, Stephen Pahoff, Nathalie Bock, Dietmar W. Hutmacher
Article
Chemistry, Applied
Nathalie Bock, Truc Le-Buu Pham, Tran Bao Nguyen, Trong Binh Nguyen, Hien A. Tran, Phong A. Tran
CARBOHYDRATE POLYMERS
(2020)
Article
Multidisciplinary Sciences
Giovannimaria Murari, Nathalie Bock, Huan Zhou, Lei Yang, Teresa Liew, Kate Fox, Phong A. Tran
SCIENTIFIC REPORTS
(2020)
Article
Biotechnology & Applied Microbiology
Silvia Cometta, Nathalie Bock, Sinduja Suresh, Tim R. Dargaville, Dietmar W. Hutmacher
Summary: Coating biodegradable polycaprolactone scaffolds with human serum albumin and tannic acid can effectively reduce Staphylococcus aureus colonization, offering a promising approach for preventing biofilm-related infections in breast implant surgery without relying on antibiotics.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Margaux Vigata, Christoph Meinert, Nathalie Bock, Bronwin L. Dargaville, Dietmar W. Hutmacher
Summary: Water in hydrogels plays a significant role in biocompatibility and hemocompatibility. The ionic strength and pH affect the mesh size of hydrogels, potentially impacting drug delivery applications, while drug loading increases non-freezable bound water. This research provides insights into molecular arrangements at the hydrogel-polymer interface and their response to environmental cues.
Article
Chemistry, Multidisciplinary
Scott Lovell, Leran Zhang, Thomas Kryza, Anna Neodo, Nathalie Bock, Elena De Vita, Elizabeth D. Williams, Elisabeth Engelsberger, Congyi Xu, Alexander T. Bakker, Maria Maneiro, Reiko J. Tanaka, Charlotte L. Bevan, Judith A. Clements, Edward W. Tate
Summary: Kallikrein-related peptidases (KLKs) play important roles in prostate cancer, with significant decoupling between KLK activity and abundance. The proteolysis of KLK should be considered as an additional parameter for accurate diagnosis and monitoring of PCa, presenting a promising therapeutic target.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Materials Science, Biomaterials
Akhilandeshwari Ravichandran, Christoph Meinert, Onur Bas, Dietmar W. Hutmacher, Nathalie Bock
Summary: Tissue engineering strategies are used to study the bone marrow microenvironment, but the effects of biomechanical stimuli on bone marrow adipose tissue (BMAT) are poorly understood. Engineered BMAT analogs in a novel bioreactor showed reduced adipokine expression following mechanical loading, providing opportunities for studying marrow physiology in greater complexity.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Cell & Tissue Engineering
Melanie Kahl, Dominik Schneidereit, Nathalie Bock, Oliver Friedrich, Dietmar W. Hutmacher, Christoph Meinert
Summary: This study introduces a software package named MechAnalyze for standardized and automated analysis of force-displacement data generated in unconfined compression tests. Comparative studies show that the software can reliably determine compressive moduli, failure stress, and failure strain of hydrogels, engineered, and native tissues, offering an intuitive user interface with minimal user input. MechAnalyze provides a fast and user-independent data analysis method, promoting process standardization, reproducibility, and comparability of mechanical characterization data in biomaterials and tissues.
TISSUE ENGINEERING PART C-METHODS
(2021)
Article
Multidisciplinary Sciences
Nathalie Bock, Thomas Kryza, Ali Shokoohmand, Joan Rohl, Akhilandeshwari Ravichandran, Marie-Luise Wille, Colleen C. Nelson, Dietmar W. Hutmacher, Judith A. Clements
Summary: The all-human microtissue-engineered model of mineralized metastatic tissue plays a crucial role in understanding the bone tumor microenvironment and treatment response in mCRPC, with enzalutamide demonstrating better therapeutic efficacy compared to bicalutamide in a mineralized microenvironment.
Article
Polymer Science
Margaux Vigata, Cathal D. O'Connell, Silvia Cometta, Dietmar W. Hutmacher, Christoph Meinert, Nathalie Bock
Summary: Hydrogels are considered ideal for local drug delivery due to their tunability, with the newly developed GelMA-DDS showing excellent drug encapsulation efficiencies and tunable mechanical properties for the delivery of cefazolin against SSIs. The diffusive and proteolytic drug delivery of cefazolin showed burst release characteristics and dose-dependent efficacy against S. aureus, highlighting the potential of GelMA-DDS for the prophylaxis and treatment of SSIs.
Article
Multidisciplinary Sciences
Sebastian Eggert, Melanie Kahl, Ross Kent, Lukas Gaats, Nathalie Bock, Christoph Meinert, Dietmar W. Hutmacher
Summary: The current manual mixing steps for viscous materials are time-consuming and can lead to irreproducibility. This study presents an automated workflow using an open source protocol designer and workstation to improve reproducibility and efficiency. The optimized workstation allows for precise pipetting and temperature control, making it suitable for a wide range of viscous materials.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)