Journal
MOLECULAR THERAPY-NUCLEIC ACIDS
Volume 24, Issue -, Pages 512-527Publisher
CELL PRESS
DOI: 10.1016/j.omtn.2021.01.035
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Funding
- Hubei Natural Science Foundation [2019CFB499]
- Scientific Research Fund of Union Hospital [02.03.2018244]
- National Natural Science Foundation of China [81900432]
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In this study, we found that SNHG16 expression was upregulated under high glucose conditions, promoting proliferation, migration, and angiogenesis of hRMECs. Functionally, SNHG16 was associated with NF-kappa B and PI3K/AKT pathways, and could sequester miR-146a-5p and miR-7-5p to regulate hRMEC dysfunction by acting as a ceRNA with IRAK1 and IRS1.
Diabetic retinopathy (DR) is a severe diabetes-induced eye disease, in which its pathological phenomena basically include abnormal proliferation, migration, and angiogenesis of micro vascular endothelial cells in the retina. Long non-coding RNAs (lncRNAs) have been proven to be important regulators in various biological processes, but their participation in DR remains largely undiscovered. In the present study, we aimed to unveil the role of lncRNA small nucleolar RNA host gene 16 (SNHG16) in regulating the functions of human retinal micro vascular endothelial cells (hRMECs) under a high-glucose (HG) condition. We found that SNHG16 expression was significantly upregulated in hRMECs treated with HG. Functionally, SNHG16 could facilitate hRMEC proliferation, migration, and angiogenesis. Moreover, SNHG16 was associated with nuclear factor kappa B (NF-kappa B) and phosphatidylinositol 3-kinase (PI3K)/AKT pathways. Mechanistically, SNHG16 could promote hRMEC dysfunction by sequestering microRNA (miR)-146a-5p and miR-7-5p to act as a competing endogenous RNA (ceRNA) with interleukin-1 receptor-associated kinase 1 (IRAK1) and insulin receptor substrate 1 (IRS1). In conclusion, our results illustrated the potential role of SNHG16 in facilitating hRMEC dysfunction under HG treatment, providing a novel approach for DR therapy.
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