Highly Efficient Leaf Base Protoplast Isolation and Transient Expression Systems for Orchids and Other Important Monocot Crops

Highly Efficient Protoplast Isolation and Transient Expression System for Functional Characterization of Flowering Related Genes in Cymbidium Orchids

(2020) Rui Ren et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Low-temperature-induced changes in the transcriptome reveal a major role of CgSVP genes in regulating flowering of Cymbidium goeringii

(2019) Fengxi Yang et al.   BMC GENOMICS

A high‐throughput transient expression system for rice

(2019) Mike T. Page et al.   PLANT CELL AND ENVIRONMENT

New insights into gibberellin signaling in regulating flowering in Arabidopsis

(2019) Shengjie Bao et al.   Journal of Integrative Plant Biology

Efficient protoplast isolation and transient gene expression system for Phalaenopsis hybrid cultivar ‘Ruili Beauty’

(2018) Jinlan Li et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single-cell mutation detection to mutant plant regeneration

(2018) Choun-Sea Lin et al.   PLANT BIOTECHNOLOGY JOURNAL

An efficient mesophyll protoplast isolation, purification and PEG-mediated transient gene expression for subcellular localization in Chinese kale

(2018) Bo Sun et al.   SCIENTIA HORTICULTURAE

A Protoplast Transient Expression System to Enable Molecular, Cellular, and Functional Studies in Phalaenopsis orchids

(2018) Hsiang-Yin Lin et al.   Frontiers in Plant Science

An Efficient Protocol for Model Legume Root Protoplast Isolation and Transformation

(2018) Ning Jia et al.   Frontiers in Plant Science

Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L.)

(2018) S. V. G. N. Priyadarshani et al.   Plant Methods

The Apostasia genome and the evolution of orchids

(2017) Guo-Qiang Zhang et al.   NATURE

Efficient isolation of Magnolia protoplasts and the application to subcellular localization of MdeHSF1

(2017) Yamei Shen et al.   Plant Methods

Establishment of transient gene expression systems in protoplasts from Liriodendron hybrid mesophyll cells

(2017) Ailing Huo et al.   PLoS One

Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis

(2016) Kalpana Nanjareddy et al.   BMC BIOTECHNOLOGY

A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants

(2016) Xu Tang et al.   Molecular Plant

An efficient transient mesophyll protoplast system for investigation of the innate immunity responses in the rubber tree (Hevea brasiliensis)

(2016) Xiaodong Zhang et al.   PLANT CELL TISSUE AND ORGAN CULTURE

UV crosslinked mRNA-binding proteins captured from leaf mesophyll protoplasts

(2016) Zhicheng Zhang et al.   Plant Methods

Protoplast isolation and development of a transient expression system for sweet cherry ( Prunus avium L.)

(2016) Liping Yao et al.   SCIENTIA HORTICULTURAE

The Dendrobium catenatum Lindl. genome sequence provides insights into polysaccharide synthase, floral development and adaptive evolution

(2016) Guo-Qiang Zhang et al.   Scientific Reports

Cell-type specific metabolic profiling of Arabidopsis thaliana protoplasts as a tool for plant systems biology

(2015) Sara V. Petersson et al.   Metabolomics

A highly efficient grapevine mesophyll protoplast system for transient gene expression and the study of disease resistance proteins

(2015) Feng-Li Zhao et al.   PLANT CELL TISSUE AND ORGAN CULTURE

An efficient PEG-mediated transient gene expression system in grape protoplasts and its application in subcellular localization studies of flavonoids biosynthesis enzymes

(2015) Huiling Wang et al.   SCIENTIA HORTICULTURAE

PEG-mediated transient gene expression and silencing system in maize mesophyll protoplasts: a valuable tool for signal transduction study in maize

(2014) Jianmei Cao et al.   ACTA PHYSIOLOGIAE PLANTARUM

The genome sequence of the orchid Phalaenopsis equestris

(2014) Jing Cai et al.   NATURE GENETICS

Efficient Transformation of Oil Palm Protoplasts by PEG-Mediated Transfection and DNA Microinjection

(2014) Mat Yunus Abdul Masani et al.   PLoS One

Gibberellin Acts Positively Then Negatively to Control Onset of Flower Formation in Arabidopsis

(2014) N. Yamaguchi et al.   SCIENCE

Transient expression for functional gene analysis using Populus protoplasts

(2013) Biyue Tan et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Functional characterization of aromatic amino acid aminotransferase involved in 2-phenylethanol biosynthesis in isolated rose petal protoplasts

(2012) Hiroshi Hirata et al.   JOURNAL OF PLANT PHYSIOLOGY

Highly Efficient Isolation of Populus Mesophyll Protoplasts and Its Application in Transient Expression Assays

(2012) Jianjun Guo et al.   PLoS One

An efficient cucumber (Cucumis sativus L.) protoplast isolation and transient expression system

(2012) Hongyu Huang et al.   SCIENTIA HORTICULTURAE

High-throughput protoplast transactivation (PTA) system for the analysis of Arabidopsis transcription factor function

(2011) Nora Wehner et al.   PLANT JOURNAL

A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes

(2011) Yang Zhang et al.   Plant Methods

History of orchid propagation: a mirror of the history of biotechnology

(2009) Tim Wing Yam et al.   Plant Biotechnology Reports

Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method

(2009) Fu-Hui Wu et al.   Plant Methods

Orchids

(2008) David L. Roberts et al.   CURRENT BIOLOGY

Efficient isolation, culture and regeneration of Lotus corniculatus protoplasts

(2008) S. V. Raikar et al.   Plant Biotechnology Reports

Protoplast isolation and transient gene expression in switchgrass,Panicum virgatum L.

(2007) Mitra Mazarei et al.   Biotechnology Journal