Article
Microbiology
Monika Kakol, Ezher Tagliasacchi, Andrzej Borkowski, Miroslaw Slowakiewicz
Summary: The nutritional condition of seed mussels used in New Zealand's Greenshell mussel aquaculture industry shows high variability, which may contribute to the marked variation observed in spat performance. Environmental factors that limit feeding during harvest and transport of spat are likely influencing the quality of spat used by the industry. Measures to improve the quality of wild spat supply, such as testing nutritional condition and developing nursery culture methods and artificial spat feeds, are needed.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Xiaoyin Gao, Liming Zheng, Yating Yao, Hailin Peng
Summary: Technological breakthrough in electron microscopy has revolutionized EM imaging resolution. Graphene, with its atomic thickness and excellent properties, has attracted attention for achieving high-resolution multi-dimensional imaging. However, preparing high-quality suspended graphene membranes remains challenging. This review explores the applications of suspended graphene membranes in multi-dimensional EM imaging and discusses their future prospects.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Biochemical Research Methods
Chidinma A. Okolo, Ilias Kounatidis, Johannes Groen, Kamal L. Nahas, Stefan Balint, Thomas M. Fish, Mohamed A. Koronfel, Aitziber L. Cortajarena, Ian M. Dobbie, Eva Pereiro, Maria Harkiolaki
Summary: This protocol describes sample preparation strategies for correlative 3D cryo-structured illumination microscopy and cryo-soft X-ray tomography, including a direct comparison and recommendations regarding fiducial selection. By combining different microscopy techniques, nanometer resolution imaging can be achieved under cryogenic conditions. Strategic use of markers is crucial for experimental design.
Article
Biochemistry & Molecular Biology
Natassa Pippa, Nefeli Lagopati, Aleksander Forys, Maria Chountoulesi, Hektor Katifelis, Varvara Chrysostomou, Barbara Trzebicka, Maria Gazouli, Costas Demetzos, Stergios Pispas
Summary: This study successfully prepared polymer-grafted hybrid liposomes using the aqueous heat method, incorporating the amphiphilic block copolymer PLMA-b-PDMAEMA and improving the physicochemical and morphological characteristics of the liposomes. The hybrid liposomes also showed higher protein loading capacity without compromising biocompatibility.
Article
Materials Science, Multidisciplinary
Y. Ikeda, R. Yuan, A. Chakraborty, H. Ghassemi-Armaki, J. M. Zuo, R. Maass
Summary: The study revealed the presence of nano-scale intermetallic phase G-phase in uncracked grain boundaries, suggesting it as one of the primary nanoscopic drivers for grain-boundary weakening and crack initiation. These findings shift the focus of LME research to the very early stages immediately following Zn diffusion, where secondary phase nucleation and growth emerge as the root-cause for failure.
MATERIALS TODAY ADVANCES
(2022)
Article
Materials Science, Multidisciplinary
Carsten Dittmayer, Hans-Hilmar Goebel, Frank L. Heppner, Werner Stenzel, Sebastian Bachmann
Summary: The study introduces a method for automated acquisition of data on all structures in EM sections, significantly improving efficiency. A sample preparation method has been established to ensure the acquisition of complete sections without artifacts. The use of LDS will greatly facilitate access to EM data for various applications.
MICROSCOPY AND MICROANALYSIS
(2021)
Article
Pharmacology & Pharmacy
E. Meziu, M. Koch, J. Fleddermann, K. Schwarzkopf, M. Schneider, A. Kraegeloh
Summary: Dehydration affects the structure of mucus, transforming it from a non-porous to porous network. Various microscopy techniques revealed the structural organization of mucus components and successfully visualized the major gel forming mucin in its hydrated state.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Review
Biochemistry & Molecular Biology
Yixin Xu, Shangyu Dang
Summary: Cryo-sample preparation is a critical step in obtaining high-resolution macromolecule structures using cryo-EM, but it is often affected by high uncertainty and low reproducibility. Developing new methods to enhance cryo-sample quality is therefore an important research topic.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Materials Science, Multidisciplinary
Hyeongjun Koh, Eric Detsi, Eric A. Stach
Summary: Understanding the nanoscale structure and chemistry of energy storage materials is crucial for battery performance, but characterizing them at high resolution can be challenging due to sample preparation methods. In this study, we used cryogenic lift-out technique to prepare air-sensitive lithium metal and investigated ion-beam damage during sample preparation. Through cryogenic transmission electron microscopy, we found that lithium was not damaged by ion-beam milling, but formed lithium oxide shells in the PFIB/SEM chamber. Cryogenic energy loss spectroscopy confirmed the oxidation of lithium during sample preparation. Our results highlight the importance of understanding how cryogenic lift-out sample preparation affects the high-resolution characterization of reactive battery materials.
MICROSCOPY AND MICROANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Abhijit H. Phakatkar, Vitaliy Yurkiv, Pankaj Ghildiyal, Yujie Wang, Azadeh Amiri, Lioudmila V. Sorokina, Michael R. Zachariah, Tolou Shokuhfar, Reza Shahbazian-Yassar
Summary: In this study, the interaction of multi-principal elemental alloy nanoparticles with Escherichia coli bacteria was evaluated using the in situ graphene liquid cell scanning transmission electron microscopy approach. The nanoparticles caused damage to the bacteria wall and resulted in cytoplasmic leakage. The release of metal ions from the nanoparticles showed selectivity, with copper ions having the highest release, followed by nickel ions, and iron ions having the lowest release. The binding affinity of bacterial cell membrane protein functional groups with the metal cations drove the selective release of metal ions from the nanoparticles.
Article
Chemistry, Physical
Hanglong Wu, Hao Su, Rick R. M. Joosten, Arthur D. A. Keizer, Laura S. van Hazendonk, Maarten J. M. Wirix, Joseph P. Patterson, Jozua Laven, Gijsbertus de With, Heiner Friedrich
Summary: The study presents methods to quantitatively map and dynamically modulate liquid layer thickness in Liquid-Phase (Scanning) Transmission Electron Microscopy (LP-(S)TEM) experiments, aiming to achieve high-resolution imaging and overcome diffusion limitations.
Article
Food Science & Technology
Amal S. Albedwawi, Reem Al Sakkaf, Tareq M. Osaili, Ahmed Yusuf, Amin Olaimat, Shao-Quan Liu, Giovanni Palmisano, Nagendra P. Shah, Mutamed M. Ayyash
Summary: This paper investigated the ability of lactic acid bacteria (LAB) and Bifidobacterium to remove acrylamide (AA) and examined the mechanism(s) of the AA removal using various microscopic techniques. The results showed that Bifidobacterium breve and Lactiplantibacillus plantarum exhibited higher acrylamide reduction rates. The study also found that both strains could tolerate low pH levels and remove acrylamide under in vitro gastrointestinal conditions.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Yawei Li, Li Wang, Yufeng He, Wei Zheng, Langhong Lou, Jian Zhang
Summary: The study found that as the temperature increases, the spacing of dislocation networks in the gamma/gamma' interface decreases, while the minimum creep rate increases non-linearly. This is due to the enhanced dislocation-climb in the interface and gamma'-cutting by a <010> superdislocations.
SCRIPTA MATERIALIA
(2022)
Article
Physics, Applied
Carles Bosch, Joerg Lindenau, Alexandra Pacureanu, Christopher J. Peddie, Marta Majkut, Andrew C. Douglas, Raffaella Carzaniga, Alexander Rack, Lucy Collinson, Andreas T. Schaefer, Heiko Stegmann
Summary: Correlative multimodal imaging is a valuable method for investigating complex structural relations in life sciences. It requires establishing sample preparation workflows that are compatible with multiple imaging techniques. This can involve imaging a fluorescently labeled region of interest in a biological soft tissue sample with light microscopy before staining the specimen with heavy metals for higher resolution structural imaging.
APPLIED PHYSICS LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Amir Peyman Soleymani, Marcia Reid, Jasna Jankovic
Summary: This research implements an epoxy-free ultramicrotome technique on beginning-of-life (BOL) and end-of-life (EOL) samples of proton exchange membrane fuel cells (PEMFCs) to observe and analyze the electrode structure in detail. Using transmission electron microscopy (TEM) and electron tomography (TEM), images of the ionomer network, carbon particles' structure, and Pt distribution are obtained, providing insights into their structural changes over time.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Environmental Sciences
Keni Cota-Ruiz, Alma B. Peregrino-Uriarte, Monserrath Felix-Portillo, Jose A. Martinez-Quintana, Gloria Yepiz-Plascencia
MARINE ENVIRONMENTAL RESEARCH
(2015)
Article
Biochemistry & Molecular Biology
Keni Cota-Ruiz, Lilia Leyva-Carrillo, Alma B. Peregrino-Uriarte, Elisa M. Valenzuela-Soto, Teresa Gollas-Galvan, Silvia Gomez-Jimenez, Jesus Hernandez, Gloria Yepiz-Plascencia
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Carlos A. Reyes-Ramos, Alma B. Peregrino-Uriarte, Keni Cota-Ruiz, Elisa M. Valenzuela-Soto, Lilia Leyva-Carrillo, Gloria Yepiz-Plascencia
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2018)
Article
Environmental Sciences
Keni Cota-Ruiz, Jose A. Hernandez-Viezcas, Armando Varela-Ramirez, Carolina Valdes, Jose A. Nunez-Gastelum, Alejandro Martinez-Martinez, Marcos Delgado-Rios, Jose R. Peralta-Videa, Jorge L. Gardea-Torresdey
ENVIRONMENTAL POLLUTION
(2018)
Article
Agriculture, Multidisciplinary
Fernando L. Garcia-Carreno, Keni Cota, Maria A. Navarrete Del Toro
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2008)
Article
Environmental Sciences
Keni Cota-Ruiz, Yossef Lopez de los Santos, Jose A. Hernandez-Viezcas, Marcos Delgado-Rios, Jose R. Peralta-Videa, Jorge L. Gardea-Torresdey
ENVIRONMENT INTERNATIONAL
(2019)
Review
Environmental Sciences
Yuqing Ye, Illya A. Medina-Velo, Keni Cota-Ruiz, Fabiola Moreno-Olivas, Jorge L. Gardea-Torresdey
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2019)
Article
Environmental Sciences
Jose A. Nunez-Gastelum, Stephanie Hernandez-Carreon, Marcos Delgado-Rios, Juan Pedro Flores-Marguez, Maria M. Meza-Montenegro, Claudia Osorio-Rosas, Keni Cota-Ruiz, Jorge L. Gardea-Torresdey
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2019)
Article
Chemistry, Multidisciplinary
Yuqing Ye, Keni Cota-Ruiz, Jose A. Hernandez-Viezcas, Carolina Valdes, Illya A. Medina-Velo, Reagan S. Turley, Jose R. Peralta-Videa, Jorge L. Gardea-Torresdey
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2020)
Article
Environmental Sciences
C. Tamez, M. Molina-Hernandez, I. A. Medina-Velo, K. Cota-Ruiz, J. A. Hernandez-Viezcas, J. Gardea-Torresdey
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Engineering, Environmental
Swati Rawat, Keni Cota-Ruiz, Haijie Dou, Venkata L. R. Pullagurala, Nubia Zuverza-Mena, Jason C. White, Genhua Niu, Nilesh Sharma, Jose A. Hernandez-Viezcas, Jose R. Peralta-Videa, Jorge L. Gardea-Torresdey
Summary: The study found that under copper compound exposure, the weathering process significantly deteriorates leaf biomass, root catalase activity, and leaf pigment content in spinach plants.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Environmental Sciences
Keni Cota-Ruiz, Yuqing Ye, Carolina Valdes, Chaoyi Deng, Yi Wang, Jose A. Hernandez-Viezcas, Maria Duarte-Gardea, Jorge L. Gardea-Torresdey
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Review
Environmental Sciences
Keni Cota-Ruiz, Marcos Delgado-Rios, Alejandro Martinez-Martinez, Jose Alberto Nunez-Gastelum, Jose Ramon Peralta-Videa, Jorge Luis Gardea-Torresdey
CURRENT OPINION IN ENVIRONMENTAL SCIENCE & HEALTH
(2018)