Journal
NUTRIENTS
Volume 13, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/nu13030800
Keywords
apoptosis; copper; DNA damage; iodine; micronutrient supplement; oxidative stress; placenta; pregnancy; selenium
Categories
Funding
- National Health and Medical Research Council (NHMRC) [GNT1161079]
- NHMRC Investigator Grant [GNT1174971]
- Flinders University Matthew Flinders Fellowship
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Imbalanced maternal micronutrient status, poor placentation, and oxidative stress are associated with greater risk of pregnancy complications. Selenium and iodine reduce DNA damage and apoptosis, while copper has no significant effect on placenta after oxidative stress induction.
Imbalanced maternal micronutrient status, poor placentation, and oxidative stress are associated with greater risk of pregnancy complications, which impact mother and offspring health. As selenium, iodine, and copper are essential micronutrients with key roles in antioxidant systems, this study investigated their potential protective effects on placenta against oxidative stress. First trimester human placenta explants were treated with different concentrations of selenium (sodium selenite), iodine (potassium iodide), their combination or copper (copper (II) sulfate). The concentrations represented deficient, physiological, or super physiological levels. Oxidative stress was induced by menadione or antimycin. Placenta explants were collected, fixed, processed, and embedded for laser ablation inductively coupled plasma-mass spectrometry (LA ICP-MS) element imaging or immunohistochemical labelling. LA ICP-MS showed that placenta could uptake selenium and copper from the media. Sodium selenite and potassium iodide reduced DNA damage and apoptosis (p < 0.05). Following oxidative stress induction, a higher concentration of sodium selenite (1.6 mu M) was needed to reduce DNA damage and apoptosis while both concentrations of potassium iodide (0.5 and 1 mu M) were protective (p < 0.05). A high concentration of copper (40 mu M) increased apoptosis and DNA damage but this effect was no longer significant after induction of oxidative stress. Micronutrients supplementation can increase their content within the placenta and an optimal maternal micronutrient level is essential for placenta health.
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