Review
Chemistry, Multidisciplinary
Andrei V. Kabashin, Vasyl G. Kravets, Alexander N. Grigorenko
Summary: Label-free optical biosensing has great potential in biomedical diagnostics, environmental and food safety, and security. However, it lacks the sensitivity of traditional labelling methods. This article explores ways to overcome the sensitivity barriers and achieve superior sensitivity in label-free biosensing through novel structural architectures, artificial materials, and the use of optical phase as a sensing parameter.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Chemistry, Analytical
Hao Huang, Chuanxiang Zhang, Jie Zhou, Dan Wei, Tingting Ma, Wenfei Guo, Xueying Liu, Song Li, Yan Deng
Summary: A label-free fipronil aptasensor was developed using Polylysine-black phosphorus nanosheets and gold nanoparticles. This sensor provides a quantitative detection method for fipronil.
Article
Biotechnology & Applied Microbiology
Guangli He, Yue Zhou, Mifang Li, Yanzhen Guo, Hang Yin, Baocheng Yang, Shouren Zhang, Yibiao Liu
Summary: An ultrasensitive photoelectrochemical (PEC) sensor based on ZnO@polydopamine/Au nanocomposites was developed for quantitative detection of A beta, showing a wide linear range and low detection limit. Additionally, the sensor exhibited good selectivity, stability, and reproducibility, providing a promising method for advanced PEC immunoassays for AD biomarkers.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Gianluigi Zito, Gennaro Sanita, Bryan Guilcapi Alulema, Sofia N. Lara Yepez, Vittorino Lanzio, Fabrizio Riminucci, Stefano Cabrini, Maria Moccia, Concetta Avitabile, Annalisa Lamberti, Vito Mocella, Ivo Rendina, Silvia Romano
Summary: Research into utilizing all-dielectric metasurfaces sustaining photonic bound states in the continuum for biosensing, combining the potential of reactive sensing principle and topological decoupling for numerical study. Achieved high sensitivity PNA/DNA selective biosensing using quasi-BIC resonator with real-time monitoring of binding events.
Article
Nanoscience & Nanotechnology
Sayan Chandra, Jared Cozart, Aritra Biswas, Sang Lee, Debashis Chanda
Summary: Early detection of IgG is crucial, and the T-MOKE technique shows significantly superior sensitivity and resolving power compared to conventional methods, with a detection limit down to 10 ng/mL. This technique has the potential to be extended to detect biologically/chemically relevant molecules at lower concentrations for early biomedical diagnosis and therapy.
Article
Nanoscience & Nanotechnology
Jie Zhou, Dan Wei, Changchun Hu, Shuo Li, Liang Ma, Zhu Chen, Yan Deng
Summary: In this study, a simple label-free aptamer-based electrochemical sensor was developed for the determination of mercury ions. Polylysine modified black phosphorus nanosheets and gold nanoparticles were used as signal amplification strategy. The experimental results showed that the sensor exhibited a wide linear range and low detection limit, and had good specificity, stability, and repeatability.
Article
Chemistry, Analytical
Jiandong Liu, Bin Liu, Juan Liu, Xing-Dao He, Jinhui Yuan, Zabih Ghassemlooy, Hamdi Torun, Yong-Qing Fu, Xuewu Dai, Wai Pang Ng, Richard Binns, Qiang Wu
Summary: A high quality factor (Q-factor) optical fiber interferometer based biosensing platform, combining single mode-core-only-single mode fiber (SCS) and multimode interference effect, is developed for ultra-high sensitivity biosensing. This biosensor is capable of effectively detecting concentrations of Staphylococcus aureus from 10 to 105 CFU/mL, with good sensitivity, stability, reproducibility and specificity, showing great potentials in diagnostics.
Article
Biophysics
Augusto Juste-Dolz, Martina Delgado-Pinar, Miquel Avella-Oliver, Estrella Fernandez, Daniel Pastor, Miguel V. Andres, Angel Maquieira
Summary: The Bio Bragg Gratings (BBGs) present a novel biosensing approach using diffractive structures of protein bioreceptors to transduce the magnitude of biorecognition assays into the intensity of single peaks in the reflection spectrum. Through theoretical and experimental studies, the design, fabrication, and optimization of this system are explored to provide insights into its bioanalytical prospects. The system shows potential for avoiding unwanted signal contributions from non-specific binding and for conceiving multiplexed BBG systems capable of performing multiple label-free biorecognition assays in a single device.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Hao Yin, Ming Lei, Hui Liu, YongPing Dong
Summary: Simultaneous anodic and cathodic electrochemiluminescence emissions were achieved using black phosphorus nanosheets and graphitic carbon nitrides as emitters, leading to the development of a biosensor for cholesterol detection. By separating the anode and cathode ECL signals, false positive signals can be significantly reduced, demonstrating excellent analytical performance with remarkable sensitivity and potential for enzymatic biosensing applications.
Article
Chemistry, Multidisciplinary
Yuanyuan Dong, Chenxing Xu, Lei Zhang
Summary: A label-free and reliable photoelectrochemical platform for highly selective monitoring of norepinephrine (NE) was developed using metallic Bi nanoparticles anchored on hollow porous ZnSnO3 microspheres (3D Bi/ZnSnO3). The unique design offered high selectivity and excellent sensitivity, enabling remarkable NE biosensing in biological fluids. This novel sensor has the potential for widespread applications in medical diagnostics and research.
Article
Biophysics
Xiaojuan Liu, Yuecan Zhao, Feng Li
Summary: This study presents an immobilization-free PEC biosensor for ultrasensitive determination of CEA, utilizing DNA-functionalized MOFs and T7 exonuclease-assisted recycling amplification. The biosensor shows a low detection limit, wide linear range, high selectivity for CEA detection, and great potential for application in bioanalysis and early disease diagnosis.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Nanoscience & Nanotechnology
Chao-Long Hu, Hui-Jing Pan, Rong-Na Ma, Shuang Cheng, Li-Ping Jia, Wei Zhang, Lei Shang, Qing-Wang Xue, Qin Wei, Huai-Sheng Wang
Summary: A photoelectrochemical sensing platform based on gold nanoparticle-attached perovskite Cs3Bi2Br9 QDs/BiOBr heterostructures was developed for sensitive detection of hydrogen peroxide. The platform showed efficient photoelectrochemical performance and promising potential for biological applications.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Valentina Mussi, Ines Fasolino, Debadrita Paria, Sara De Simone, Maria Caporali, Manuel Serrano-Ruiz, Luigi Ambrosio, Ishan Barman, Maria Grazia Raucci, Annalisa Convertino
Summary: Black phosphorus nanosheets (2D BP) were visualized and monitored in cancer cells using optical diffraction tomography (ODT) and Raman spectroscopy, which provided insights into their internalization and chemical changes. This label-free morpho-molecular approach offers a powerful tool for studying the pharmacokinetic properties of engineered nanomaterials in preclinical research.
Article
Chemistry, Analytical
Natasha Mandal, Anindita Das, Rakesh S. Moirangthem
Summary: This work introduces a label-free optical biosensing platform that combines localized surface plasmon resonance (LSPR) with in-situ ellipsometry technique. The sensor chip, fabricated via the thermal dewetting method, consists of partially embedded Au-Ag alloy nanoparticles on a glass substrate. Spectroscopic ellipsometry was used to characterize the sensor chip and determine its optical constant. The proposed sensor chip demonstrates high stability, surface sensitivity, non-destructiveness, label-free detection, ease of fabrication, and cost-effectiveness, making it suitable for large-scale production and commercialization in biomedical applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Beatrix Peter, Barbara Majoros, Sandor Kurunczi, Andrea Violetta Acs, Inna Szekacs, Szilvia Bosze, Gabor M. Kovacs, Imre Boldizsar, Robert Horvath
Summary: Label-free grating-coupled interferometry (GCI) biosensing method was used to reveal the specific biomolecular interactions between two novel aryltetralin lignans and human serum albumin (HSA). The results suggest the potential medical application of these lignans without further optimization of their binding affinity to HSA.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)