Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 756, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2020.143859
Keywords
Prothioconazole; Toxicity; Gene expression; Apoptosis
Categories
Funding
- Program of Innovative Entrepreneurship Training for Undergraduate of China [201910341003]
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This study found that prothioconazole is toxic to zebrafish embryos, causing malformations and apoptosis. Exposure to high concentrations of prothioconazole up-regulated the expression levels of oxidative stress defense-related genes and p53, leading to apoptosis in the early life stages of zebrafish.
Triazole fungicides are extensively applied in general agriculture for fungal control and have negative impacts on aquatic organisms. Prothioconazole, a widely used triazole fungicide, is toxic to zebrafish, but systematic research on the negative effects caused by prothioconazole in zebrafish embryos is limited. In this study, we studied the developmental toxicology, oxidative stress and apoptosis caused by prothioconazole in zebrafish embryos. Exposure to 0.850 ma prothioconazole impacts embryo survival and hatching. Prothioconazole exposure caused embryo malformation, especially yolk-sac and pericardial edemas, and prothioconazole-induced apoptosis was observed. Additionally, exposure to a high prothioconazole concentration up-regulated the expression levels of oxidative stress defense-related genes and p53. The bax to bcl2 ratio increased along with exposure time and prothioconazole concentration. Prothioconazole induced apoptosis during the early life stages of zebrafish and may trigger oxidative-stress and p53-dependent pathway responses. Our findings increase our understanding of the molecular mechanisms of oxidative stress and cell death caused by prothioconazole. (C) 2020 Elsevier B.V. All rights reserved.
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