Negative impacts of realistic doses of spherical and irregular microplastics emerged late during a 42 weeks-long exposure experiment with blue mussels

Microplastics have been found in all compartments of the environment, and numerous life forms are known to take up the anthropogenic particles. Marine filter feeders are particularly susceptible to ingest suspended microplastics, but long-term studies on the potential effects of this uptake are scarce. We exposed juvenile Mytilus spp. to environmentally realistic doses of irregularly shaped polyvinylchloride (PVC) particles (15, 1500, 15,000, 150,000, 1,500,000 particles/individual/week calibrated in the size range 11-60 mu m) and regularly shaped polystyrene (PS) beads (15, 1500, 15,000 particles/individual/week, 40 mu m) over 42 weeks. During this period, we monitored physiological traits such as clearance rate, byssus production, growth rate, superoxide dismutase (SOD) activity, malondialdehyde (MDA) concentrations, and the condition index (CI). Negative effects of the tested microplastics on mussel performance emerged late in the experiment and were rather weak. Interestingly, even after having received the lowest particle dose of PS, SOD activity in the gill was significantly lower in mussels exposed to microplastics compared to a group of conspecifics that were kept in clean water. However, growth and CI, which are both closely related to the fitness of the mussels, were not found to be impaired at the end of the exposure phase. This is the so far longest laboratory microplastic exposure study on mussels and we worked with particle doses that reflect todays pollution levels. The small effect sizes we observed for the response variables assessed suggest that these specific microplastics pose only a minor threat to blue mussel populations. (c) 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Automated summary

This study exposed juvenile mussels to environmentally realistic doses of PVC and PS microplastics for 42 weeks, finding minimal negative effects on mussel performance, with lower SOD activity in gills of mussels exposed to PS even at the lowest dose. Growth and CI were not impaired, indicating that these specific microplastics pose only a minor threat to blue mussel populations at current pollution levels.