Journal
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
Volume 123, Issue -, Pages 68-77Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envexpbot.2015.10.002
Keywords
Abiotic stresses; Expression pattern; Late embryogenesis abundant protein; OsLEA4 gene; Rice
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Funding
- National Genetically Modified Organisms Breeding Major Projects of China [2009ZX08009-109B]
- National Key Technology R & D Program of China [2011BAD35B 02-05]
- Natural Science Foundation Project of Chongqing Science and Technology Committee (CSTC) [2012jjA80009]
- National 111 Plan Base [B06023]
- Public Experiment Center of State Bioindustrial Base (Chongqing), China
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Late embryogenesis abundant (LEA) proteins are involved in tolerance to drought, cold and high salinity in many different organisms. In this study, real-time PCR was used to analyze the expression pattern of the OsLEA4 gene in rice, which showed that OsLEA4 was expressed in different organ tissues during different development stages of rice. The expression levels of OsLEA4 in the leaves during the tillering stage and leaves and panicles during the heading stage, the filling stage and the full ripe stage were dramatically increased. Moreover, based on seed germination, growth status and physiological indices, the overexpression of OsLEA4 in transgenic rice plants conferred increased resistance to drought, salt and heavy metal stresses compared with the wild type (WT) plants. After drought, high salinity and heavy metal stresses, the increase in the malondialdehyde content in transgenic plants was less than that in WT, and the increase in the free proline and soluble sugar contents in transgenic plants were greater than those of the WT plants. These results indicated that the OsLEA4 protein contributes to drought, salt and heavy metal tolerance in rice plants. Thus, OsLEA4 is a potential candidate gene for plant genetic modification. (C) 2015 Published by Elsevier B.V.
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