Article
Plant Sciences
Guohui Li, Cheng Song, Muhammad Aamir Manzoor, Daoyuan Li, Yunpeng Cao, Yongping Cai
Summary: This study identified three PAL genes in the pear genome and revealed their significant impact on stone cell and lignin contents in pear fruits. Additionally, a transcription factor PbWLIM1 that regulates the transcription level of PbPAL2 was also discovered.
Article
Plant Sciences
Kazem Ghassemi-Golezani, Salar Farhangi-Abriz
Summary: Biochar reduces lignification and enhances nutrient uptake by plant roots. Addition of biochar promotes root growth, activity, and the absorption of essential elements, while reducing oxidative damages caused by toxic substances.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Javed Ahmad, Arlene A. Ali, Asma A. Al-Huqail, M. Irfan Qureshi
Summary: The study demonstrated that foliar spray of triacontanol was effective in alleviating drought stress in Brassica juncea by regulating the antioxidant system, calcium levels, and lignification.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Dilara Maslennikova, Sergey Ivanov, Svetlana Petrova, Guzel Burkhanova, Igor Maksimov, Oksana Lastochkina
Summary: This study investigated the effects of nitric oxide (NO) on wheat seedling growth under normal and salinity conditions. It was found that exogenous NO contributed to the accumulation of endogenous salicylic acid (SA) and improved plant growth. Under salinity stress, pretreatment with NO activated the phenylpropanoid metabolism, thereby reducing the negative effects of salinity stress on plant growth.
Article
Biochemistry & Molecular Biology
Yu Qin, Qiue Li, Qiuju An, Dexin Li, Sipei Huang, Yongyang Zhao, Weijia Chen, Jiayu Zhou, Hai Liao
Summary: Drought is a key threat to plants and agriculture, and the role of the enzyme phenylalanine ammonia lyase (PAL) in drought tolerance has been explored in this study. A PAL member called FuPAL1 was isolated from the alpine pharmaceutical plant Fritillaria unibracteata. FuPAL1 was found to play a role in the ammonization process and was involved in substrate binding and catalysis. Transgenic Arabidopsis plants with FuPAL1 showed increased levels of lignin and salicylic acid and enhanced drought tolerance. These findings suggest that FuPAL1 could be a valuable target for improving drought resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Feiyan Xue, Zihui Liu, Yue Yu, Yangjie Wu, Yuxin Jin, Mingfeng Yang, Lanqing Ma
Summary: Researchers cloned the cDNA of Rhodotorula glutinis PAL (RgPAL) and introduced codon optimization to enhance its protein expression and enzyme activity in Escherichia coli. This improvement allowed for the successful expression and purification of the recombinant enzyme PAL-opt, which exhibited high specific activity for substrate L-phenylalanine and L-tyrosine. This bifunctional enzyme provides a promising foundation for future applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Hassan S. Al-Zahrani, Hesham E. Alharby, Khalid Rehman Hakeem, Reiaz Ul Rehman
Summary: The study found that the application of zinc can alleviate the negative effects of salt stress on plant growth to a certain extent by improving physiological and biochemical changes, including increased shoot and root length, enhanced chlorophyll and carotenoid contents, and increased enzymatic antioxidant activities.
Article
Plant Sciences
Morag E. Ferguson, Rodney P. Eyles, Ana Luisa Garcia-Oliveira, Fortunus Kapinga, Esther A. Masumba, Teddy Amuge, Jessen V. Bredeson, Daniel S. Rokhsar, Jessica B. Lyons, Trushar Shah, Steve Rounsley, Geoffrey Mkamilo
Summary: This study detected QTL associated with CBSD and identified key genes involved in the resistance of cassava to CBSD. The findings are significant for improving breeding efficiency.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Physical
Chun-Yen Hsieh, Yi-Hao Huang, Hui-Hsuan Yeh, Pei-Yu Hong, Che-Jen Hsiao, Lu-Sheng Hsieh
Summary: Bambusa oldhamii BoPAL4 exhibits broad substrate specificity, with optimal reaction pH and temperature varying for different substrates. A major substrate specificity site mutant, BoPAL4-H123F, shows improved affinity for L-phenylalanine. BoPAL4 is confirmed to utilize L-phenylalanine, L-tyrosine, and L-DOPA as substrates.
Article
Agriculture, Multidisciplinary
Sandrine S. Ferreira, Pedro Silva, Amelia M. Silva, Fernando M. Nunes
Summary: The chemical composition and enzymatic activity of the main Portuguese elderberry cultivars were determined for the first time, revealing the decisive influence of climatic conditions on the maturation and quality of elderberries.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Engineering, Environmental
Jialian Wei, Sisi Liao, Muzi Li, Bin Zhu, Hongcheng Wang, Lei Gu, Huayan Yin, Xuye Du
Summary: Cadmium is a toxic heavy metal that poses a serious threat to crop safety and human health. This study investigated the molecular mechanisms underlying the response of Aegilops tauschii, a common wheat donor, to cadmium stress. Key genes involved in cadmium stress response were identified, including AetSRG1, which played a crucial role in reducing cadmium accumulation and increasing antioxidant production. These findings provide new insights into the development of low cadmium wheat.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Plant Sciences
AiLian Liu, Yue Zhu, YuHao Wang, TianYu Wang, ShuPing Zhao, Kai Feng, LiangJun Li, Peng Wu
Summary: In this study, PAL genes in E. ferox were identified and functionally analyzed. EfPAL1 and EfPAL2 were found to be key genes in flavonoid biosynthesis, and a regulatory network for EfPAL2 was established. These findings provide novel information for understanding the mechanism of flavonoid biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Fangyu Mo, Long Li, Chao Zhang, Chenghui Yang, Gong Chen, Yang Niu, Jiaxin Si, Tong Liu, Xinxin Sun, Shenglan Wang, Dongdong Wang, Qin Chen, Yue Chen
Summary: This study identified 14 StPAL genes in potato for the first time and investigated their expression patterns under different stresses and hormone treatments. The results suggest that these genes may be involved in stress defense and chemical defense mechanisms in potato.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Fariborz Habibi, Ali Sarkhosh, Fabian Guillen, Maria Serrano, Daniel Valero
Summary: The effects of glycine betaine (GB) and methyl salicylate (MeSA) treatments, alone and in combination, on the chilling tolerance and bioactive compounds of blood orange cv. Moro during cold quarantine storage were investigated. The combined MeSA and GB treatment showed the highest effectiveness in enhancing the fruit's chilling tolerance and preserving bioactive compounds.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yannik Brack, Chenghai Sun, Dong Yi, Uwe T. Bornscheuer
Summary: p-Coumaric acid (p-CA) is a key precursor for flavonoid biosynthesis, and tyrosine ammonia lyases (TALs) catalyze the synthesis of p-CA from l-tyrosine. In this study, 875 putative TALs and 46 putative phenylalanine/tyrosine ammonia lyases (PTALs) were analyzed, and 5 TALs and 3 PTALs were characterized and found to exhibit the proposed enzymatic activity. The TAL from Chryseobacterium luteum sp. nov (TAL(clu)) showed the highest affinity and conversion efficiency towards l-tyrosine. Optimization of reaction conditions resulted in p-CA yields of 2.03 g/L after 8 hours by TAL(clu) and 2.35 g/L after 24 hours by TAL from Rivularia sp. PCC 7116 (TAL(rpc)). These TALs are potential candidates for the production of flavonoid precursor p-CA using whole-cell systems.
Review
Energy & Fuels
Danielly C. I. Martarello, Aline M. Almeida, Renata C. Sinzker, Dyoni M. Oliveira, Rogerio Marchiosi, Wanderley D. dos Santos, Osvaldo Ferrarese-Filho
Summary: Biofuels produced from lignocellulosic biomass have positive impacts on energy security and greenhouse gas emissions reduction. However, lignocellulosic byproducts can affect the production of cellulosic ethanol. Lignin, a major factor in biomass recalcitrance, can be reduced through gene and enzyme regulation, allowing for improved saccharification. Altering the structure and composition of lignin through heterologous gene insertion can also enhance saccharification efficiency.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Plant Sciences
Flavia Carolina Moreira-Vilar, Hingrid Ariane da Silva, Rogerio Marchiosi, Rita de Cassia Siqueira-Soares, Aline Finger-Teixeira, Karla Gabriela da Silva, Isabela de Carvalho Contesoto, Rodrigo Polimeni Constantin, Wanderley Dantas dos Santos, Osvaldo Ferrarese-Filho
Summary: Herbicide-resistant plants have evolved rapidly due to the repeated use of active ingredients with limited mechanisms of action. This study evaluated the effects of p-methoxycinnamic acid on the growth, respiration, and lignification of the wild poinsettia weed. The results showed that p-methoxycinnamic acid inhibited root growth and cell viability, disrupted cellular respiration, and increased oxidative stress and lignin synthesis. These findings suggest that p-methoxycinnamic acid has phytotoxic potential and may be explored as a potential herbicide.
Article
Plant Sciences
Tiara da Silva Coelho-Bortolo, Rogerio Marchiosi, Ana Paula Ferro, Rita de Cassia Siqueira-Soares, Rodrigo Polimeni Constantin, Wanderley Dantas dos Santos, Osvaldo Ferrarese-Filho
Summary: l-DOPA affects the ability of soybean plants to uptake and assimilate inorganic nitrogen into amino acids. It inhibits the uptake and conversion of nitrate and ammonium and leads to the accumulation of free amino acids in the roots and leaves.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Agricultural Engineering
Wanderley D. dos Santos, Diego E. R. Gonzaga, Victor Hugo Salvador, Denis L. Freitas, Breno M. Joia, Dyoni M. Oliveira, Debora C. C. Leite, Graciene S. Bido, Aline Finger-Teixeira, Amanda P. de Souza, Maria de Lourdes T. M. Polizeli, Rodrigo P. Constantin, Rogerio Marchiosi, Fabiano A. Rios, Osvaldo Ferrarese-Filho, Marcos S. Buckeridge
Summary: The use of natural compounds can significantly increase lignocellulosic saccharification, leading to higher bioethanol production and positively impacting energy security and sustainability.
BIOMASS & BIOENERGY
(2023)
Letter
Plant Sciences
Dyoni M. Oliveira
Editorial Material
Plant Sciences
Dyoni M. Oliveira, Igor Cesarino
Article
Agronomy
Viviane Fatima de Oliveira, Lucas Figueiredo, Gabriel de Oliveira Correia, Maria de Fatima Pires da Silva Machado, Hugo Zeni Neto, Wanderley Dantas dos Santos, Claudete Aparecida Mangolin
Summary: This study demonstrates that the application of natural inhibitors can increase the production of fermentable sugars in energy crops without the need for additional lignin-removal pretreatments. The most successful results soften the most recalcitrant lignocellulose to the level of other biomasses and make it more susceptible to saccharification.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2023)
Article
Plant Sciences
Mariana Conceicao de Souza, Renato Polimeni Constantin, Josielle Abrahao, Marcela de Paiva Foletto-Felipe, Luiz Henryque Escher Grizza, Rodrigo Polimeni Constantin, Wanderley Dantas dos Santos, Osvaldo Ferrarese-Filho, Rogerio Marchiosi
Summary: The discovery of new herbicides is a significant challenge in modern agriculture, with only a small number of assessed molecules showing promising characteristics. This study found that 3-cyanobenzoic acid inhibited the growth of maize seedlings by inducing oxidative stress and increasing antioxidant enzyme activity and respiratory sensitivity.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Review
Plant Sciences
Aline Marengoni Almeida, Rogerio Marchiosi, Josielle Abrahao, Rodrigo Polimeni Constantin, Wanderley Dantas dos Santos, Osvaldo Ferrarese-Filho
Summary: The shikimate pathway is crucial for developing antimicrobial agents and herbicides. By using bioinformatics tools, enzyme inhibitors of this pathway can be explored efficiently. This article reviews the characterization of enzymes in this pathway and updates the status of their inhibitors in microorganisms and plants.
PHYTOCHEMISTRY REVIEWS
(2023)
Article
Toxicology
Paulo Francisco Veiga Bizerra, Fernanda Sayuri Itou da Silva, Eduardo Hideo Gilglioni, Leticia Fernanda Nanami, Eduardo Makiyama Klosowski, Byanca Thais Lima de Souza, Ana Flavia Gatto Raimundo, Karina Borba Paulino dos Santos, Juliana Moraes Mewes, Renato Polimeni Constantin, Marcio Shigueaki Mito, Emy Luiza Ishii-Iwamoto, Jorgete Constantin, Fabio Erminio Mingatto, Giovana Natiele Machado Esquissato, Roerio Marchiosi, Wanderley Dantas dos Santos, Osvaldo Ferrarese-Filho, Rodrigo Polimeni Constantin
Summary: Clomipramine, a tricyclic antidepressant, has been associated with acute hepatotoxicity. It hinders mitochondrial functioning, which may affect energy metabolism in the liver. This study used various experimental systems to examine the effects of clomipramine on mitochondrial functions in the liver. The findings confirmed that clomipramine impairs metabolic processes and cellular structures, particularly membranes, in the liver by disrupting mitochondrial functions.
TOXICOLOGY LETTERS
(2023)
Article
Agronomy
Thais Valerio Raimundo Menino, Breno Miguel Joia, Aline Marengoni Almeida, Francisco Carlos Krzyzanowski, Rogerio Marchiosi, Osvaldo Ferrarese-Filho
Summary: Soybean seeds are crucial for global food production, but mechanical damage can affect their quality. The composition of lignin monomers in the seed coat plays a crucial role in the resistance of soybean seeds to mechanical damage, providing a new method for identifying resistant soybean seeds.
JOURNAL OF SEED SCIENCE
(2023)
Article
Plant Sciences
Wanli You, Jinglin Zhang, Xueyin Ru, Feng Xu, Zhengguo Wu, Peng Jin, Yonghua Zheng, Shifeng Cao
Summary: This study investigated the effect of calcium chloride (CaCl2) treatment on GABA accumulation in fresh-cut cantaloupe and the underlying mechanisms. The results showed that CaCl2 treatment increased GABA content and the activities of GAD and SSADH enzymes, while reducing glutamate content and GABA-T activity. Additionally, CaCl2 treatment upregulated the expressions of CmCML11 and CmCAMTA5, as well as several GABA shunt genes, through the transcriptional activation by CmCAMTA5. Furthermore, the interaction between CmCML11 and CmCAMTA5 enhanced the transcriptional activation of GABA shunt genes. Overall, this study reveals that CaCl2 treatment promotes GABA accumulation in fresh-cut cantaloupe through the combined effect of CmCML11 and CmCAMTA5 in regulating the expressions of GABA shunt genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Min Wang, Yupeng Wang, Xiaohui Wang, Guangwei Wei, Huiyi Yang, Xi Yang, Tinghai Shen, Huijie Qu, Sheng Fang, Ziming Wu
Summary: This study identified the high nitrogen use efficiency (NUE) black sesame variety 17-156 and analyzed its underlying physiological and molecular mechanisms. The results showed that 17-156 possesses a sophisticated nitrogen metabolizing machinery to uptake and assimilate higher quantities of inorganic nitrogen, simultaneously improving carbon metabolism and growth. Many important genes were up-regulated in 17-156 under high nitrogen condition. Additionally, 38 potential candidate genes were identified for future studies to improve sesame's NUE. These findings provide valuable resources for understanding the regulatory network of nitrogen metabolism and developing sesame cultivars with improved NUE.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
He Dong, Chongmei Xu, Chengtao Zhang, Li Zhang, Yaqin Yao, Suiqi Zhang
Summary: The study found that short cells in maize leaves not only improve leaf mechanical support and photosynthetic performance, enhance drought resistance, but also participate in stomatal regulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Na Liu, Wenyan Shang, Mengxin Guan, Jibin Xiao, Guangxiang Tian, Baozhan Ma, Wenjing Shang, Xu Li, Shijia Zhao, Chuang Li, Kun Cheng, Wenming Zheng
Summary: This study cloned the full-length cDNA sequence of TaSPX3 gene in wheat and found that TaSPX3 responds to low phosphorus stress in multiple wheat genotypes. Overexpressing TaSPX3 can alleviate phosphorus deficiency symptoms and promote plant growth in Arabidopsis. The study also revealed the interaction of TaSPX3 with other genes related to the phosphorus starvation signaling pathway.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Kongyuan Wu, Lizhen Wang, Zihan Wu, Ziqing Liu, Zengfei Li, Jun Shen, Shengjie Shi, Hong Liu, Christopher Rensing, Renwei Feng
Summary: Selenium (Se) can reduce uptake and translocation of cadmium (Cd) in plants by regulating root morphology. This study investigated the effects of Se(IV) on root exudates, root morphology, root endogenous hormones, and Cd uptake efficiency in rice under Cd stress. The results showed that Se(IV) significantly reduced Cd concentrations in shoots and roots, and decreased Cd uptake efficiency via root hairs. Se(IV) also affected root morphology, root exudates, and the synthesis of hormones like IAA and JA. However, transcriptome analysis revealed no upregulated differentially expressed genes (DEGs) in IAA synthesis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Di Wang, Huaifang Zhang, Xuefei Hu, Haizhen Zhang, Shuang Feng, Aimin Zhou
Summary: This study identified a cell number regulator gene called SlCNR8 in willow, which enhances resistance to trace metals in transgenic poplar seedlings. SlCNR8 reduces Cd uptake and accumulation, and can be used as a candidate gene for genetic improvement of phytostabilisation of trace metals.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)