Article
Entomology
Mathew S. Baite, S. R. Prabhukarthikeyan, S. Raghu
Summary: This study evaluated the effectiveness of various biocontrol agents in controlling false smut disease in rice. It was found that Trichoderma harzianum and Bacillus subtilis showed the highest potential in controlling the disease. In addition to disease suppression, these biocontrol agents also improved rice yield.
Article
Biochemistry & Molecular Biology
Yu Zhang, Xinyue Li, Shuya Zhang, Tianling Ma, Chengxin Mao, Chuanqing Zhang
Summary: A quantitative loop-mediated isothermal amplification (q-LAMP) method was developed for Ustilaginoidea virens detection and quantification, which is more sensitive and efficient than quantitative real-time PCR (q-PCR) method. The q-LAMP assay could detect a concentration of 6.4 spores/mL at an optimal reaction temperature of 63.4℃ within 60 minutes. It is a powerful and simple monitoring tool for U. virens, providing valuable technical support for the forecast and management of rice false smut.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Plant Sciences
Dewei Yang, Niqing He, Fenghuang Huang, Yidan Jin, Shengping Li
Summary: Rice false smut (RFS), caused by Ustilaginoidea virens, is a devastating disease worldwide, resulting in significant yield loss and health threats due to mycotoxin production. This review updates the understanding of RFS symptoms, resistance genes, genomics, effectors, and immune response mechanisms. Additionally, it explores the identification methods, breeding for resistance, and interactions between effector proteins and resistance genes, which can contribute to the development of disease-resistant materials in rice breeding programs.
Article
Microbiology
Rongtao Fu, Cheng Chen, Jian Wang, Yao Liu, Liyu Zhao, Daihua Lu
Summary: This study focused on the morphology, pathogenicity, mating-type loci distribution, and genetic characterization of different isolates of Ustilaginoidea virens. A total of 221 strains were collected from 13 rice-growing regions in southwest China. The results showed high morphological diversity and significant differentiation in pathogenicity among the isolates. Mating-type analysis revealed both heterothallic and homothallic forms, and genetic analysis indicated a high level of genetic diversity with no clustering based on geographical origin.
Article
Agriculture, Multidisciplinary
Xiaojie Li, Liangxiong Xu, Zhencheng Lv, Fengming Li, Jinghua Xue, Yonghong Peng, Xiaoyi Wei, Ling Li
Summary: Ustilaginoidea virens is a pathogenic fungus that causes false smut disease in rice. A new trisaccharide ester called MTE-1 showed significant inhibitory activity against U. virens by inhibiting hyphal growth and disrupting the endomembrane system. This inhibition was achieved through altering lipid synthesis, primary metabolic pathways, and intracellular redox dyshomeostasis. The findings provide a foundation for the development of antifungal agents.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Jiandong Bao, Rong Wang, Shilei Gao, Zhe Wang, Yu Fang, Lv Wu, Mo Wang
Summary: In this study, a near-complete genome assembly of U. virens isolate UV-FJ-1 was generated using PacBio Sequel II HIFI-read sequencing technology, revealing a large number of protein-coding genes and secondary metabolite biosynthesis gene clusters, which will be valuable resources for further understanding the biological and pathogenic mechanisms of this fungus.
Article
Microbiology
Mina Yu, Junjie Yu, Huijuan Cao, Xiayan Pan, Tianqiao Song, Zhongqiang Qi, Yan Du, Shiwen Huang, Yongfeng Liu
Summary: This study identified and characterized two velvet genes in the rice false smut pathogen Ustilaginoidea virens. The deletion of these genes affected the conidiation and glucose utilization of the pathogen. These findings provide insights into the regulatory mechanisms underlying rice false smut ball formation.
Article
Microbiology
Mina Yu, Junjie Yu, Huijuan Cao, Tianqiao Song, Xiayan Pan, Zhongqiang Qi, Yan Du, Rongsheng Zhang, Shiwen Huang, Wende Liu, Yongfeng Liu
Summary: The study demonstrated that UvSUN1 is essential for the growth, cell wall integrity, and pathogenicity of Ustilaginoidea virens, providing new insights into the function of SUN family proteins in the growth and pathogenesis of this pathogen.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shanshan Qiu, Anfei Fang, Xinhang Zheng, Shanzhi Wang, Jiyang Wang, Jing Fan, Zongtao Sun, Han Gao, Jiyun Yang, Qingtao Zeng, Fuhao Cui, Wen-Ming Wang, Jianping Chen, Wenxian Sun
Summary: In this study, a crucial virulence effector, SCRE4, was identified and characterized in U. virens. SCRE4 suppressed the expression of the immune positive modulator OsARF17, contributing to the infection process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Manas Kumar Bag, Anuprita Ray, Prahlad Masurkar, B. N. Devanna, C. Parameswaran, Mathew Baite, Prakash Chandra Rath, Amaresh Nayak
Summary: This study is the first to report on the genetic diversity and population structure of U. virens from India, with 63 isolates from the vast eastern and north-eastern regions. Seventeen RAPDs and 14 SSRs were identified as polymorphic, with a total of 140 alleles detected. All isolates were grouped into two major clusters, showing partial geographical segregation, supported by principal coordinate analysis. Mantel test suggested genetic distance increased with geographical distance, with more genetic variation within populations than among populations.
Article
Agronomy
Amrita Banerjee, M. K. Bag, Adarsh Kumar Chandra, Somnath Roy, S. Raghu, N. P. Mandal
Summary: A recombinase polymerase amplification (RPA) assay protocol was developed to directly detect Ustilaginoidea virens from rice spikelet. The RPA assay efficiently detected U. virens from both genomic DNA and mycelial crude extract. The developed RPA assay will be effective for field monitoring of U. virens.
Article
Agronomy
Meng Shuai, Qiu Jiehua, Xiong Meng, Liu Zhiquan, Jane Sadhna Jagernath, Lin Fucheng, Shi Huanbin, Kou Yanjun
Summary: In this study, a general stress response factor UvWHI2 was identified in Ustilaginoidea virens, and it was found to be up-regulated during infection, affecting fungal growth and pathogenicity. Knockout of UvWhi2 resulted in decreased formation of secondary spores and compromised pathogenicity of U. virens, indicating the importance of UvWHI2 in stress responses and secondary spore formation in this pathogen.
Article
Biochemistry & Molecular Biology
Mengyao Xue, Siji Zhao, Gan Gu, Dan Xu, Xuping Zhang, Xuwen Hou, Jiankun Miao, Hai Dong, Dongwei Hu, Daowan Lai, Ligang Zhou
Summary: Rice false smut caused by Villosiclava virens is a highly destructive fungal disease that reduces the yield and quality of rice grains. This study isolated an albino strain LN02 from white false smut balls collected in China. The genome analysis revealed the deficiency of ustilaginoidin biosynthesis in strain LN02, which led to albinism. This finding highlights the potential of strain LN02 in the development and application of secondary metabolites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Kritika Rana, Jagjiwan Mittal, Jagriti Narang, Annu Mishra, Ramesh Namdeo Pudake
Summary: The study developed a sensitive and selective oxidized graphene-based geno-biosensor for the detection of rice false smut, with probe DNA on paper electrodes and oxidized graphene enhancing detection limit and sensitivity. The device successfully detected up to 10 fM of target ssDNA, showing potential for cost-effective biosensors for detecting other plant pathogens.
PLANT PATHOLOGY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
H. Sharanabasav, D. Pramesh, M. K. Prasannakumar, E. Chidanandappa, M. K. Yadav, U. Ngangkham, B. Parivallal, B. T. Raghavendra, C. Manjunatha, S. K. Sharma, N. Karthik
Summary: The study characterized the geo-distinct isolates of Ustilaginoidea virens for morpho-molecular and mating-type locus diversity, revealing significant diversity among isolates from Southern India and distribution of heterothallic mating types in paddy fields. Complete synteny conservation between Indian and Chinese strains was observed at the MAT1 locus.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Plant Sciences
X. Y. Liu, C. Chaisiri, Y. Lin, Y. P. Fu, W. X. Yin, F. X. Zhu, J. B. Li, B. Xiong, H. Wu, A. Xu, C. X. Luo
Summary: Citrus melanose, caused by the fungus Diaporthe citri, is a major disease in China. The study evaluated the use of ecofriendly chemicals for disease control, finding that low doses of certain chemicals were effective in inhibiting the infection process. The use of these ecofriendly chemicals could play an important role in the future management of citrus melanose.
Article
Energy & Fuels
Yuxiang Gao, Xi Jin, Liumei Teng, Sohrab Rohani, Minyu He, Jiangling Li, Shan Ren, Qingcai Liu, Junbin Huang, Huamei Duan, Yuntao Xin, Weizao Liu
Summary: This study proposes a novel process using copperas, a solid waste, to extract magnesium and nickel from laterite ore for simultaneous CO2 mineralization and recovery of nickel. The copperas is decomposed into SO2, sulfating the laterite ore and the addition of Na2SO4 facilitates the formation of low melting point substances, enhancing the extraction efficiency. The heat of sulfation compensates the heat of copperas decomposition, reducing overall energy consumption. The carbonation experiments reveal the optimal CO2 storage capacity of approximately 291 kg.t? 1 laterite ore. Compared to the conventional acid-based Mg extraction process, this cheap method avoids reagent recycling and reduces the amount of alkali used.
Article
Energy & Fuels
Hongli Wu, Liumei Teng, Weizao Liu, Handan Zhang, Junbin Huang, Qingcai Liu
Summary: Manganese carbonate ore is the most abundant manganese ore in China, but the mining and beneficiation process generates a large amount of hazardous manganese tailings. This study investigated the effects of calcination temperature on the denitration performance and physicochemical properties of manganese tailings, and found that the best catalytic activity can be achieved at a calcination temperature of 500°C. Manganese tailings can be effectively utilized for reducing NO emissions.
Article
Agronomy
Yueran Liu, Jinsong Qu, Yufu Wang, Weixiao Yin, Chaoxi Luo
Summary: A bZIP protein called UvATF21 was found to play important regulatory roles in Ustilaginoidea virens, including in vegetative growth, conidiation, stress response, and virulence. This study provides important insights into the physiological processes of this pathogenic fungus.
Article
Biochemistry & Molecular Biology
Xiaoyang Chen, Qiutao Xu, Yaping Yue, Yuhang Duan, Hao Liu, Xiaolin Chen, Junbin Huang, Lu Zheng
Summary: Pathogen attack increases reactive oxygen species (ROS) levels in plants, which activate plant defense mechanisms. Our study used a quantitative proteomics approach to map oxidized cysteine sites in rice leaves and identified proteins involved in various biological processes, including defense response pathways. We found that infection by Magnaporthe oryzae increased oxidative modification levels of cysteine sites in ribosomal proteins, promoting the translational efficiency of mRNAs involved in defense responses. Our findings reveal a novel role of post-translational modifications and provide valuable resources for future research on protein redox regulation.
PLANT COMMUNICATIONS
(2023)
Article
Computer Science, Artificial Intelligence
Junchao Zhang, Yidong Luo, Junbin Huang, Ying Liu, Jiayi Ma
Summary: A multi-exposure image fusion method based on perception enhanced structural patch decomposition (PESPD-MEF) is proposed, which decomposes and fuses image patches to generate informative and perception-realistic results. The method outperforms state-of-the-art methods in terms of perceptual realism for both multi-exposure images and single low-light image enhancement.
INFORMATION FUSION
(2023)
Editorial Material
Plant Sciences
Hanfei Liu, Yanyan Li, Xihong Li, Hao Liu, Junbin Huang, Lu Zheng
Review
Oncology
Yong Liu, Zefan Du, Junbin Huang, Tianwen Li, Jing Zhang, Yixian Li, Wenfang Yi, Chun Chen
Summary: Ferroptosis, an iron-dependent programmed cell death, has been discovered in recent years. It is characterized by the accumulation of lipid reactive oxygen species in cells, leading to oxidative stress and cell death. It plays a pivotal role in normal physiology and the development of various diseases.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Junbin Huang, Guanhua Hu, Pan Suo, Lu Bai, Yifei Cheng, Yu Wang, XiaoHui Zhang, KaiYan Liu, YuQian Sun, LanPing Xu, Jun Kong, ChenHua Yan, Xiaojun Huang
Summary: A retrospective study on 25 pediatric AMKL patients without DS was conducted, and the results showed that patients with trisomy 19 and MRD negative pre-HSCT had better outcomes. Therefore, haploidentical HSCT may be an option for high-risk AMKL patients without DS.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Qin Zhou, Lifen Huang, Yong Liu, Junbin Huang, Luping Wen, Jing Yang, Jintang Liang, Yun Chen, Chun Chen
Summary: This study analyzed the transcriptome of patients with aplastic anemia (AA) from peripheral blood through single-cell sequencing and revealed the affected metabolic pathways. It was found that the metabolic abnormalities of T lymphocytes mainly focused on glycolysis/gluconeogenesis, while those of natural killer cells concentrated in oxidative phosphorylation. Key genes involved in abnormal metabolic processes were identified through differential gene expression analysis. The activation of tumor necrosis factor superfamily, member 12 (TNFSM12) in tumor necrosis factor (TNF) signaling was broadly observed in AA, suggesting that targeting immune cell's abnormal metabolic pathways may contribute to the development of novel treatment strategies.
FRONTIERS IN ONCOLOGY
(2023)
Article
Microbiology
Lifan Gu, Yufu Wang, Songlin Xie, Yueran Liu, Jiali Yan, Weixiao Yin, Chaoxi Luo
Summary: Ustilaginoidea virens causes destructive panicle disease in rice, threatening agricultural production. UvATG6 is necessary for autophagy and contributes to growth, conidiation, and virulence in U. virens. This study demonstrates the important role of UvATG6 in U. virens and its interaction with UvBI-1 and UvBI-1b proteins, suppressing Bax-induced cell death.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Fei Fan, Yong-Xu Zhu, Min-Yi Wu, Wei-Xiao Yin, Guo-Qing Li, Matthias Hahn, Mohamed S. S. Hamada, Chao-Xi Luo
Summary: Gray mold, caused by B. cinerea, leads to significant losses in fruit and vegetable production. The use of AP fungicides has been effective in controlling this disease, but the development of resistance poses new challenges. This study identified a specific gene mutation that confers resistance to AP fungicides, shedding light on the mechanism of resistance.
MICROBIOLOGY SPECTRUM
(2023)
Article
Engineering, Electrical & Electronic
Sitong Chen, Junbin Huang, Wen Liu, Hongcan Gu
Summary: In this study, the effects of rotation on the reflection spectrum of a polyimide-coated Fibre Bragg Grating (FBG) for real-time temperature monitoring of a generator or electric motor rotor were analyzed using formulas. The measurement error resulting from low-speed operation was evaluated using the finite element method. Furthermore, the key design of the FBG sensors and fiber optic rotary joint (FORJ) mating part were investigated, and the reliability of the measurement system was tested by monitoring the temperature of a rotating shaft.
OPTICAL FIBER TECHNOLOGY
(2023)
Article
Engineering, Environmental
Hongli Wu, Weizao Liu, Jun Cao, Junbin Huang, Qingcai Liu
Summary: Mn-Ti supported Ce catalysts were synthesized and their denitration performance and SO2 resistance were investigated. The modified catalyst showed improved low temperature catalytic performance and excellent SO2 resistance. The presence of Ce oxides improved the NH3-SCR performance and retained more oxidation performance after being poisoned by SO2.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Deng Chen, Xuan Cai, Junjie Xing, Shen Chen, Juan Zhao, Zhiguang Qu, Guotian Li, Hao Liu, Lu Zheng, Junbin Huang, Xiao-Lin Chen
Summary: In the rice blast fungus, a lipid droplet-associated protein Nem1 was found to play a crucial role in the biogenesis and utilization of lipid droplets. Nem1 was highly expressed in conidia, but lowly expressed in appressoria, and its encoded protein was localized to lipid droplets. Deletion of NEM1 resulted in reduced numbers of lipid droplets and decreased content of diacylglycerol (DAG) or triacylglycerol (TAG).