Article
Biochemistry & Molecular Biology
Jing Guo, Long Wei, Shan-Shan Chen, Xue-Wei Cai, Yin-Na Su, Lin Li, She Chen, Xin-Jian He
Summary: Through affinity purification and mass spectrometry, we identified 28 conserved MEDIATOR subunits in Arabidopsis thaliana, including previously unvalidated subunits. Our study revealed that MED34, MED35, MED36, and MED37 are not Arabidopsis MEDIATOR subunits, while the CBP/p300 histone acetyltransferases HAC1 and HAC5 are plant-specific MEDIATOR subunits. The association of MEDIATOR with HAC1/5 and the co-regulation of gene expression by MED8/25 and HAC1/5 affect flowering time and floral development.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Junfeng Chen, Yuma Katada, Kousuke Okimura, Taiki Yamaguchi, Ying-Jey Guh, Tomoya Nakayama, Michiyo Maruyama, Yuko Furukawa, Yusuke Nakane, Naoyuki Yamamoto, Yoshikatsu Sato, Hironori Ando, Asako Sugimura, Kazufumi Tabata, Ayato Sato, Takashi Yoshimura
Summary: Coastal organisms, such as grass puffers, synchronize their reproduction with the lunar cycle. The secretion of prostaglandin E2 (PGE2) into the seawater during spawning triggers the trembling behavior of grass puffers, which is synchronized with the lunar-regulated beach-spawning behavior. The semilunar transcription factor CCAAT/ enhancer-binding protein d (cebpd) plays a role in the transcription regulation of gonadotropin-releasing hormone 1 (gnrh1) in grass puffers.
Editorial Material
Plant Sciences
Fanourios Mountourakis, Ioannis H. Hatzianestis, Stella Stavridou, Peter Bozhkov, Panagiotis N. Moschou
Summary: Biomolecules can exist in different forms, including biomolecular condensates, which are mesoscale assemblies formed through phase separation. Phase separation concentrates or sequesters specific molecules, affecting their abundance and other features in the phases and consequently influencing biochemical reactions. Recent research has implicated biomolecular condensates in the regulation of biochemical reactions in plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Chemistry, Applied
Tao Ye, Xing Chen, Qiang Li, Zhina Chen, Lin Lin, Zhi Zheng, Jianfeng Lu
Summary: This study investigated the effects of substituting myofibrillar protein with plant protein (wheat, soy, rice, and pea protein) on the gelling properties of hybrid surimi/plant protein (HSP) gels. The results showed phase separation occurred as the substitution ratio increased, with legume proteins having better compatibility to surimi and resulting in tighter and more uniform gel structures. The hydrophobicity differences between cereal and legume proteins affected the conformation and thermal aggregation of myosin. No subunit-level cross-linking between myofibrillar proteins and plant proteins was found. Pea protein mixed gels had a higher abundance of hydrophobic interactions, disulfide bonds, and β-sheet content. Dynamic light scattering found that legume protein mixed gels had smaller oversized aggregates. Small angle X-ray scattering could be used to monitor the internal phase behavior of HSP gels.
FOOD HYDROCOLLOIDS
(2023)
Article
Multidisciplinary Sciences
Liliana Felicia Iannucci, Anna Maria D'Erchia, Ernesto Picardi, Daniela Bettio, Filippo Conca, Nicoletta Concetta Surdo, Giulietta Di Benedetto, Deborah Musso, Cristina Arrigoni, Marco Lolicato, Mauro Vismara, Francesca Grisan, Leonardo Salviati, Luciano Milanesi, Graziano Pesole, Konstantinos Lefkimmiatis
Summary: The study reveals an unexpected mechanism by which EPAC1 enters the nucleus and regulates the transcription of a histone cluster by forming biomolecular condensates in its proximity in response to cAMP.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Akshaya Arva, Yasar Arfat T. Kasu, Jennifer Duncan, Mosleh A. Alkhatatbeh, Christopher S. Brower
Summary: This study reveals that Liat1 participates in nucleolar liquid-liquid phase separation regulated by Jmjd6, and also found that the lysyl-hydroxylase activity of Jmjd6 affects the function of Liat1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Tanja Zahn, Zihao Zhu, Niklas Ritoff, Jonathan Krapf, Astrid Junker, Thomas Altmann, Thomas Schmutzer, Christian Tueting, Panagiotis L. Kastritis, Steve Babben, Marcel Quint, Klaus Pillen, Andreas Maurer
Summary: Novel exotic ELF3 alleles have been discovered and shown to be associated with agronomic traits in barley. These findings suggest a central role for ELF3 in barley adaptation.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Plant Sciences
Chen Chen, Xinglin Du
Summary: LEC transcription factors play a crucial role in regulating plant embryonic development, seed maturation, and growth. Current research focuses on the role of LECs in connecting different developmental stages and generational cycles in plants, as well as their potential to control plant growth stages through epigenetic modifications.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Engineering, Mechanical
Zefan Shao, Qihan Liu
Summary: This paper reports a novel mechanism for reversible hydrogel adhesion based on osmocapillary phase separation. When two hydrogel surfaces contact, the interfacial liquid fills the gap and generates adhesion. The osmocapillary adhesion can reach 0.1 MPa strength, be reversible over 50 cycles, and be stable over 24 hours of constant loading.
EXTREME MECHANICS LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Sayantan Chatterjee, Yelena Kan, Mateusz Brzezinski, Kaloian Koynov, Roshan Mammen Regy, Anastasia C. Murthy, Kathleen A. Burke, Jasper J. Michels, Jeetain Mittal, Nicolas L. Fawzi, Sapun H. Parekh
Summary: The authors show that thermal trigger can lead to formation of at least two distinct liquid condensed phases of the FUS LC domain. Forming FUS LC condensates directly at low temperature leads to metastable, kinetically trapped condensates showing arrested coalescence, which can be escaped via thermal annealing to untrapped condensates. This study demonstrates two unique liquid FUS condensates with different molecular structures and dynamics.
Article
Multidisciplinary Sciences
Xiaofeng Chu, Xin Su, Mingdong Liu, Li Li, Tianhao Li, Yicheng Qin, Guoliang Lu, Lei Qi, Yunhui Liu, Jinzhong Lin, Qing-Tao Shen
Summary: This study investigates the effect of annealing on biological macromolecules using the 70S ribosome as a model. Experimental results show that annealing with a fast cooling rate can enhance the homogeneity and resolution of the ribosome, reaching a minimum energy state in the free-energy landscape. This approach has potential applications in improving protein stability and synchronizing proteins on a single-molecule level.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Applied
Thierry Hellebois, Frederic Addiego, Claire Gaiani, Alexander S. Shaplov, Christos Soukoulis
Summary: This study investigated the functionality of two plant seed polysaccharides, flaxseed gum (FG) and alfalfa galactomannan (AAG), in milk protein-based cryogels. The inclusion of polysaccharides in the cryogels improved thermal stability and reduced water disintegration rates. AAG specifically showed a notable reduction in monolayer water content compared to FG, possibly through self-association during cryogenic processing. FG enhanced the properties of cryogels through electrostatic complexation with proteins.
CARBOHYDRATE POLYMERS
(2024)
Article
Biochemistry & Molecular Biology
Charles E. Deckard, Jonathan T. Sczepanski
Summary: Thymine DNA glycosylase (TDG) has the ability to directly alter chromatin structure through physical interactions with DNA, inducing decompaction and condensation of chromatin fibers. This process is reversible with factors like GADD45a and sensitive to the methylation status of DNA, implying dynamic control of chromatin architecture by TDG and its binding partners.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Jianping Guo, Huiying Wang, Wei Guan, Qin Guo, Jing Wang, Jing Yang, Yaxin Peng, Junhan Shan, Mingyang Gao, Shaojie Shi, Xinxin Shangguan, Bingfang Liu, Shengli Jing, Jing Zhang, Chunxue Xu, Jin Huang, Weiwei Rao, Xiaohong Zheng, Di Wu, Cong Zhou, Bo Du, Rongzhi Chen, Lili Zhu, Yuxian Zhu, Linda L. Walling, Qifa Zhang, Guangcun He
Summary: Plants use certain receptors and proteins to enhance their resistance to insects, but the exact mechanisms underlying this process have been unclear. This study identifies a specific salivary protein produced by brown planthoppers that is detected by a plant immune receptor. Furthermore, it uncovers a mechanism involving selective autophagy that regulates this interaction. This research has significant implications for the development of high-yield, insect-resistant crops.
Article
Multidisciplinary Sciences
Matthew Charman, Nicholas Grams, Namrata Kumar, Edwin Halko, Joseph M. Dybas, Amber Abbott, Krystal K. Lum, Daniel Blumenthal, Elene Tsopurashvili, Matthew D. Weitzman
Summary: Biomolecular condensates formed by phase separation can compartmentalize and regulate cellular processes. Evidence suggests that membraneless subcellular compartments in virus-infected cells also form by phase separation. Here we demonstrate that phase separation of the human adenovirus 52-kDa protein plays a critical role in the coordinated assembly of infectious progeny particles. Our findings identify essential requirements for coordinated assembly of progeny particles and demonstrate that phase separation of a viral protein is critical for production of infectious progeny during adenovirus infection.
Article
Microbiology
Jiaming Jin, Qianqian Zhou, Fangren Lan, Junying Li, Ning Yang, Congjiao Sun
Summary: The internal quality of eggs is crucial for human consumption and embryonic development. The composition and differences in microbiota between egg yolks and whites, as well as the migration of microbes from egg white to yolk during incubation, have been explored in this study.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Nutrition & Dietetics
Bowen Yang, Shimeng Huang, Ning Yang, Aizhi Cao, Lihong Zhao, Jianyun Zhang, Guoxian Zhao, Qiugang Ma
Summary: This study found that bile acids can promote the absorption of fat-soluble vitamins, even under low-fat diets. Through experiments, it was found that bile acids can emulsify fat and promote its hydrolysis, and can improve the digestion and absorption rate of vitamin A. In the experiment, feeding diets supplemented with 60 mg/kg bile acids significantly improved laying performance in laying hens and promoted the growth of follicles in the ovaries. In addition, bile acids can also improve the utilization rate of fat and energy, and enhance the activity of lipase and lipoprotein lipase in the small intestine. Furthermore, the addition of bile acids can increase the vitamin A content in the liver and serum of laying hens, improve serum immune indicators, and antioxidant capacity.
FRONTIERS IN NUTRITION
(2022)
Article
Biotechnology & Applied Microbiology
Jing Yang, Zhiqiong Mao, Xiqiong Wang, Jingjie Zhuang, Sijia Gong, Zhouyang Gao, Guiyun Xu, Ning Yang, Congjiao Sun
Summary: The study comprehensively investigated the regulatory mechanisms of eggshell brownness in different eggshell color and time following oviposition. Results suggest that Pp IX is synthesized de novo and stored in the shell gland, with ALAS1 identified as a key gene regulating Pp IX synthesis. Additionally, three transporters in the Pp IX pathway and three metabolites in shell glands and uterine fluid may influence eggshell browning.
Article
Agriculture, Dairy & Animal Science
Qiang Huang, Chaoliang Wen, Wei Yan, Congjiao Sun, Shuang Gu, Jiangxia Zheng, Ning Yang
Summary: Improving feed efficiency is an important goal in chicken breeding and production. This study found that the development of the gizzard, liver, and cecum is related to the feed efficiency of chickens.
Article
Agriculture, Dairy & Animal Science
Qianqian Zhou, Fangren Lan, Shuang Gu, Guangqi Li, Guiqin Wu, Yiyuan Yan, Xiaochang Li, Jiaming Jin, Chaoliang Wen, Congjiao Sun, Ning Yang
Summary: In this study, the researchers investigated the effects of host genetics and gut microbiota on feed efficiency in laying hens. They found that genetic factors and gut microbiota both play important roles in determining feed efficiency, with RFI having the highest microbial influence. They also identified three microbial genera that were negatively correlated with RFI and positively contributed to improving feed efficiency.
Article
Genetics & Heredity
Xiuan Zhang, Jianbo Li, Xiqiong Wang, Yuchen Jie, Congjiao Sun, Jiangxia Zheng, Junying Li, Ning Yang, Sirui Chen
Summary: In this study, a sex-reversed chicken flock was created by injecting an aromatase inhibitor, fadrozole, and high-resolution profiles of gene expression and chromatin accessibility in embryonic gonads were constructed. The results showed that fadrozole affected the transcriptional activities of genes related to sex determination and differentiation and regulated the expression of genes related to gonadal development through nearby open chromatin regions. After sexual maturity, the sex-reversed chickens were found to be infertile, and promising genes related to spermatogenesis and infertility were identified through RNA-seq. This study provides important insights into the molecular basis of sex differentiation and infertility in sex-reversed chickens and lays a foundation for further research on bird sex development.
EPIGENETICS & CHROMATIN
(2023)
Article
Agriculture, Dairy & Animal Science
Xingzheng Li, Jianlou Song, Xuefeng Shi, Mingyi Huang, Lei Liu, Guoqiang Yi, Ning Yang, Guiyun Xu, Jiangxia Zheng
Summary: The study revealed the crucial roles of liver FMO3 and cecal microbes in TMA metabolism in ducks, with the limited oxidation ability of FMO3 explaining the weakening of TMA metabolism in ducks.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Biology
Junxiao Ren, Zhengzheng Hu, Quanlin Li, Shuang Gu, Fangren Lan, Xiqiong Wang, Jianbo Li, Junying Li, Liwa Shao, Ning Yang, Congjiao Sun
Summary: Chicken embryonic diapause is a state of temporary arrest of embryonic development triggered by cold stress-induced IRF1 expression, mediated by the PKC-NF-kappa B signaling pathway. This is in contrast to mammalian diapause which is mTOR-dependent. The study characterized the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in different diapause states and identified key regulators of diapause.
Review
Biochemistry & Molecular Biology
Xiuan Zhang, Jianbo Li, Sirui Chen, Ning Yang, Jiangxia Zheng
Summary: Sex determination and differentiation in birds involves genetic factors controlling the sex determination program, while sex differentiation is influenced by hormones and can be reversed when disturbed. The regulatory mechanism of avian cell autonomous sex identity and sex development is still not clearly understood and requires further exploration. However, avian sex research findings have provided theoretical bases for gender control technologies in poultry production, which are being improved through interdisciplinary cooperation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Chaoliang Wen, Qinli Gou, Shuang Gu, Qiang Huang, Congjiao Sun, Jiangxia Zheng, Ning Yang
Summary: This study investigated the composition of microbial communities in chicken muscle tissue and their relationship with meat quality. The results showed that the microbial composition in muscle tissue can affect the fat content, and there is also a correlation between fat content in different muscle tissues. These findings provide important insights for improving meat quality in chickens.
Article
Agriculture, Dairy & Animal Science
Yiming Yuan, Gang Zheng, Zhen You, Lulu Wang, Ziyi Wang, Congjiao Sun, Changjun Liu, Xianyao Li, Peng Zhao, Yongqiang Wang, Ning Yang, Ling Lian
Summary: This study used whole genome bisulfite sequencing (WGBS) and RNA-seq to identify genes playing important roles in the tumor transformation of Marek's disease (MD). 8 genes, including CD4 and HMGB1, were found to be negatively correlated with methylation and showed high expression in the MDV-infected group. Overexpression of CD4 and HMGB1 significantly promoted MDCC-MSB-1 cell proliferation, suggesting their potential promoting roles in MD tumorigenesis.
Article
Multidisciplinary Sciences
Zhangyuan Pan, Ying Wang, Mingshan Wang, Yuzhe Wang, Xiaoning Zhu, Shenwen Gu, Conghao Zhong, Liqi An, Mingzhu Shan, Joana Damas, Michelle M. Halstead, Dailu Guan, Nares Trakooljul, Klaus Wimmers, Ye Bi, Shang Wu, Mary E. Delany, Xuechen Bai, Hans H. Cheng, Congjiao Sun, Ning Yang, Xiaoxiang Hu, Harris A. Lewin, Lingzhao Fang, Huaijun Zhou
Summary: This study identified and characterized regulatory elements in the chicken genome by integrating genome-wide sequencing datasets from 23 adult tissues. It annotated 1.57 million regulatory elements, predicted enhancer-gene pairs, and super-enhancers. These findings have significant implications for understanding gene regulation in chicken domestication, selection, and complex trait regulation.
Article
Biotechnology & Applied Microbiology
Xinwei Jiang, Boxuan Zhang, Fangren Lan, Conghao Zhong, Jiaming Jin, Xiaochang Li, Qianqian Zhou, Junying Li, Ning Yang, Chaoliang Wen, Congjiao Sun
Summary: Blood biochemical indicators are regulated by both host genetics and gut microbiota, and their interrelationships can affect growth traits in chickens. The heritability and microbiability of these indicators vary, and several candidate genes and microbial species have been identified as potentially involved in their regulation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Ziyi Wang, Yiming Yuan, Gang Zheng, Meng Sun, Qinyuan Wang, Junfeng Wu, Junying Li, Congjiao Sun, Yongqiang Wang, Ning Yang, Ling Lian
Summary: This study reveals the distribution of ALVE in 11 chicken breeds, including the identification of 23 novel ALVEs in Chinese domestic breeds. Most of these insertion sites are located in intergenic regions and introns.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Chaoliang Wen, Chunning Mai, Ronglang Cai, Qinli Gou, Boxuan Zhang, Junying Li, Congjiao Sun, Ning Yang
Summary: Duration of fertility (DF) is an important economic trait in poultry production, and its correlation with egg production and breeding efficiency has been analyzed. The heritability of DF traits is low, but improvement of DF is possible through selection. Fertility duration day (FDD) is an effective selection criterion for improving DF, and it can be incorporated into a breeding program to enhance reproductive efficiency.
GENETICS SELECTION EVOLUTION
(2022)