Journal
ENERGY SOURCES PART A-RECOVERY UTILIZATION AND ENVIRONMENTAL EFFECTS
Volume 38, Issue 15, Pages 2170-2180Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15567036.2015.1030050
Keywords
Bioethanol production; enrichment; isolation; molasses
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In an attempt to produce bioethanol as a renewable and natural energy resource and as a promising alternative/complement to conventional petrol (i.e., gasoline), 44 microbial isolates (12 yeast and 32 bacterial strains) were isolated from molasses samples obtained from some of the sugar factories in Egypt. Among the microbial isolates obtained, only two yeast isolates (HSC-22 and HSC-24) were selected from sugarcane molasses (SCM) for their high bioethanol fermentation capabilities, recording bioethanol production of approximate to 9.6 and 8.2 g/L with actual yield of 0.48 and 0.41 g ethanol/g SCM, respectively, within 48-h incubation period at 30 degrees C. Phylogenetic identification of these isolates was performed based on the analysis of the nucleotide sequence of the 18S rDNA gene, which indicated that these isolates can be identified as Pichia veronae and Candida tropicalis, respectively, with similarity of 99%.
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