Article
Biotechnology & Applied Microbiology
Zhenxian Li, Haimei Zhu, Hao Liu, Dayue Liu, Jianhe Liu, Jiazheng Jiang, Yi Zhang, Zhang Qin, Yijia Xu, Yuan Peng, Bin Liu, Yun Long
Summary: In this study, a biomimetic nanoliposome loaded with Evolocumab (a PCSK9 inhibitor) was developed using nano-materials to alleviate atherosclerosis. The results showed that this nanoliposome can inhibit the phenotypic transition, excessive proliferation, and migration of VSMCs. Additionally, it significantly decreased the expression of PCSK9 in serum and VSMCs within the plaque of ApoE(-/-) mice.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Jia Peng, Cheng-Gang Zhu, Jian-Jun Li
Summary: Increasing data suggest that PCSK9 may play a role in the development of type 2 diabetes and be associated with clinical outcomes in diabetic patients, but there is currently no general agreement about the association of PCSK9 with T2DM. The utility of circulating PCSK9 concentration as a predictor for the risk of new-onset T2DM should be considered clinically prudent.
CARDIOVASCULAR DIABETOLOGY
(2021)
Article
Medical Laboratory Technology
Jia-ni Xu, Ting-ting Wang, Hong Shu, Shun-yi Shi, Li-chan Tao, Jian-Jun Li
Summary: Diabetes mellitus (DM) is strongly associated with atherosclerotic cardiovascular disease (ASCVD), and the protein PCSK9 has been identified as a key regulator of LDL-C levels and a potential target for improving cardiovascular outcomes. Recent studies have also linked PCSK9 to glucose metabolism, and clinical trials suggest that PCSK9 inhibitors are more effective in treating patients with DM. This review summarizes the current findings on the association between PCSK9 and glucose metabolism, including the effects of PCSK9 genetic mutations, plasma PCSK9 concentrations, and glucose-lowering drugs on metabolic parameters, as well as the impact of PCSK9 inhibitors on cardiovascular outcomes in patients with DM.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Vittoria Cammisotto, Francesco Baratta, Paola G. Simeone, Cristina Barale, Enrico Lupia, Gioacchino Galardo, Francesca Santilli, Isabella Russo, Pasquale Pignatelli
Summary: PCSK9 is involved in cholesterol metabolism and plays a role in the development of atherosclerosis and atherothrombotic processes, promoting plaque formation and oxidative stress.
Review
Biochemistry & Molecular Biology
Alessandro Morotti, Cristina Barale, Elena Melchionda, Isabella Russo
Summary: This article discusses the association between oxidative stress and hypercholesterolemia, as well as platelet hyperreactivity, and explores the impact of lipid-lowering therapies on thrombogenesis, emphasizing the significance of oxidative stress-related mechanisms in platelet function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Gilberto Vargas-Alarcon, Oscar Perez-Mendez, Hector Gonzalez-Pacheco, Julian Ramirez-Bello, Rosalinda Posadas-Sanchez, Galileo Escobedo, Jose Manuel Fragoso
Summary: Genetic variants of the PCSK7 gene are significantly associated with the development of ACS and dyslipidemia, potentially increasing the risk of ACS.
Article
Cardiac & Cardiovascular Systems
Alex K. K. Leung, Yuan Chao Xue, Antyrah de Guzman, Guilherme Grzelkovski, HyeJin Julia Kong, Kelly R. Genga, James A. Russell, John H. Boyd, Gordon A. Francis, Keith R. Walley
Summary: This study found that PCSK9 reduces the uptake of LPS in vascular endothelial cells through LDLR-mediated endocytosis, leading to a decrease in the inflammatory response. This suggests that PCSK9 inhibition may have additional effects on vascular endothelial inflammation.
Article
Immunology
Miao Yu, Wenjing Tang, Wei Liang, Baikang Xie, Ran Gao, Peiwu Ding, Xiaoying Gu, Min Wang, Shuang Wen, Peng Sun
Summary: Proprotein convertase subtilisin kexin type 9 (PCSK9) was initially known for its role in regulating cholesterol metabolism, but recent studies have shown its involvement in inflammatory and autoimmune diseases unrelated to cholesterol alterations. This study aims to investigate the roles and mechanisms of PCSK9 in myocarditis and found that PCSK9 inhibition can ameliorate the severity of myocarditis by reducing Th17 cell differentiation. PCSK9 appears to be a promising target for treating myocarditis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Coen van Solingen, Scott R. Oldebeken, Alessandro G. Salerno, Amarylis C. B. A. Wanschel, Kathryn J. Moore
Summary: The discovery of PCSK9 and its role in LDL regulation has led to the rapid development of therapeutic PCSK9 inhibitors. Research has identified certain microRNAs that can reduce PCSK9 expression, leading to increased LDLR expression in hepatic cells, which may have therapeutic implications for manipulating LDLR expression and cholesterol levels in the liver.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cell Biology
Liuxin Ning, Yanting Zou, Shuyu Li, Yue Cao, Beili Xu, Shuncai Zhang, Yu Cai
Summary: The study aimed to investigate the mechanism of anti-PCSK9 treatment on liver fibrosis by inhibiting hypoxia-induced autophagy. The results showed that anti-PCSK9 treatment could alleviate liver inflammation and fibrosis by regulating the AMPK/mTOR/ULK1 signaling pathway to reduce hypoxia-induced autophagy in hepatocytes.
Review
Cardiac & Cardiovascular Systems
Yimo Zhou, Weiqi Chen, Meng Lu, Yongjun Wang
Summary: This meta-analysis found a significant association between high circulating PCSK9 concentration and the risk of MACEs, with a linear dose-response relationship observed. However, no significant correlation was found with stroke or all-cause mortality. Further well-designed studies are needed to explore the relationships between PCSK9 concentration and stroke and mortality.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Oliver C. McGehee, Hassan Y. Ebrahim, Ashkan H. Rad, Khaldoun S. Abdelwahed, Ethar A. Mudhish, Judy A. King, Iman E. Helal, Sharon A. Meyer, Khalid A. El Sayed
Summary: Pseurotin A (PsA), a natural compound extracted from Aspergillus and Penicillium species, has shown to suppress the expression and protein-protein interaction (PPI) of PCSK9, leading to an anti-hypercholesterolemic effect. In vitro toxicity studies on non-tumor prostate and colon cells revealed high cellular death at higher concentrations of PsA, while acute organ toxicity was not observed in mice following oral dosing.
Article
Medical Laboratory Technology
Antonin Jabor, Tereza Vackova, Zdenek Kubicek, Jitka Komrskova, Marek Protus, Janka Franekova
Summary: The study evaluated the biological variation of serum PCSK9, finding a high within-subject variability in healthy individuals, suggesting that common reference intervals can be used to interpret its values.
CLINICA CHIMICA ACTA
(2021)
Review
Pharmacology & Pharmacy
Fang Jia, Si-Fan Fei, De-Bing Tong, Cong Xue, Jian-Jun Li
Summary: PCSK9 increases circulating LDL-C levels by degrading LDL receptors and has direct effects on atherosclerosis and coronary plaque inflammation. Emerging data show higher PCSK9 concentrations in women than men, suggesting potential sex differences in PCSK9 roles and clinical implications.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Rabia Aydogan Baykara, Pinar Diydem Yilmaz, Mevlut Hakan Goktepe, Cengiz Kadiyoran, Mustafa Ogul, Adem Kucuk, Medine Cumhur Cure, Erkan Cure
Summary: There is a strong independent association between subclinical atherosclerosis and PCSK9 in patients with Behcet's disease. However, there may be no independent association between PCSK9 and disease activity.
CLINICAL AND EXPERIMENTAL HYPERTENSION
(2022)
Article
Plant Sciences
Hyo-Moon Cho, Mi Zhang, Eun-Jin Park, Ba-Wool Lee, Yeon-Joo Park, Hyun-Woo Kim, Ha-Thanh-Tung Pham, Young-Won Chin, Won-Keun Oh
Summary: This study discovered several flavonostilbenes from the stems of Rhamnoneuron balansae, and evaluated their effects on SIRT1 deacetylase activity. One compound showed promising anti-aging activity in vitro.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Plant Sciences
Huong Pham, Thi Phuong Doan, Hyun Woo Kim, Tae Wan Kim, So-Yeon Park, Hangnun Kim, Mina Lee, Ki Hyun Kim, Won Keun Oh, Young Woon Lim, Kyo Bin Kang
Summary: Microbial cocultivation is a strategy to induce the biosynthesis of specialized metabolites. In this study, the coculture of Phellinus orientoasiaticus and Xylodon flaviporus was found to induce the production of multiple metabolites. The isolated compounds did not exhibit antifungal activity or cytotoxicity.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Chemistry, Multidisciplinary
Hyun Woo Kim, Chen Zhang, Garrison W. Cottrell, William H. Gerwick
Summary: The identification of metabolites from complex biofluids and tissue extracts is crucial for understanding metabolic profiles. NMR spectroscopy, particularly H-1-C-13 HSQC NMR spectra, is commonly used in metabolomics studies. However, accurate identification of individual metabolites can be challenging due to high peak intensity and similar chemical shifts. This study demonstrates the successful application of a convolutional neural network (CNN) for accurate peak identification in complex mixtures, showing better performance compared to other NMR-based metabolomic tools.
MAGNETIC RESONANCE IN CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Pisey Pel, Hee-Sung Chae, Piseth Nhoek, Young-Mi Kim, Chae-Yeong An, Hunseung Yoo, Minseok Kang, Hyun Woo Kim, Young Hee Choi, Young-Won Chin
Summary: This study investigated bioactive molecules from Sophora tonkinensis that inhibit PCSK9 expression. A new compound was identified and its absolute configuration was determined. The compound inhibited PCSK9 expression by down-regulating HNF1 alpha and SREBPs.
Article
Biochemistry & Molecular Biology
Phuong Linh Nguyen, Ahmed Elkamhawy, Young Hee Choi, Chang Hoon Lee, Kyeong Lee, Jungsook Cho
Summary: The study evaluated the antitumor effects of a series of CA inhibitors on various cancer cell lines, identifying two compounds with potent anticancer activity against breast cancer cells MCF-7 and SK-BR-3, especially in combination with DOX, demonstrating synergistic cytotoxic effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Seungchan An, Seok Young Hwang, Junpyo Gong, Sungjin Ahn, In Guk Park, Soyeon Oh, Young-Won Chin, Minsoo Noh
Summary: In this study, a machine-learning model was developed to predict the adiponectin-secretion-promoting activity of flavonoid-associated phytochemicals (FAPs). By establishing a structure-activity relationship model and a multiple linear regression model, the adiponectin activity of FAPs was successfully predicted and validated through experimental validation, providing a computational reconstruction method for phenotype-based screening systems.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Plant Sciences
Young-Won Chin, Piseth Nhoek, Sungjin Ahn, Pisey Pel, Young-Mi Kim, Jungmoo Huh, Hyun Woo Kim, Minsoo Noh
Summary: Five new quinoline alkaloids (1, 2, 4, 8, and 9) and three new coumarins (15, 17, and 19), along with 20 known compounds, were identified from a methanol extract of Orixa japonica leaves. The structures were determined using NMR spectroscopic data analysis. Compound 19's absolute configuration was proposed using electronic circular dichroism calculation. Compound 4 and 12 were found to stimulate adiponectin secretion in human bone marrow mesenchymal stem cells, with EC50 values of 13.8 and 25.8 mu M, respectively. Compound 4 and 12 were also identified as selective PPARy agonists based on PPARy binding assay and molecular modeling.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Pharmacology & Pharmacy
Byoung Hoon You, Mingoo Bae, Seung Yon Han, Jieun Jung, Kiwon Jung, Young Hee Choi
Summary: The pharmacokinetic feasibility of the stability-enhanced SESS-TD crystal was evaluated and found to be comparable to TDF, with no changes observed even after 12 months of storage. This suggests that the SESS-TD crystal may have enough pharmacokinetic feasibility to replace TDF.
Correction
Plant Sciences
Huong T. Pham, Thi Phuong Doan, Hyun Woo Kim, Tae Wan Kim, So-Yeon Park, Hangun Kim, Mina Lee, Ki Hyun Kim, Won Keun Oh, Young Woon Lim, Kyo Bin Kang
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Pharmacology & Pharmacy
Seo-Yeon Kim, Byung Hoon You, Mingoo Bae, Seung Yon Han, Kiwon Jung, Young Hee Choi
Summary: This study aimed to characterize the pharmacokinetic profiles of co-amorphous dispersions of Mirabegron (MBR) in rats and mice. The results showed that these co-amorphous dispersions can improve the relative bioavailability and area under the curve of MBR.
Article
Pharmacology & Pharmacy
Jina Kim, Young-Won Chin
Summary: The prevalence of antimicrobial-resistant bacteria poses a global challenge. Methicillin-resistant Staphylococcus aureus (MRSA), a leading cause of infections, forms biofilms on medical devices, making them more resistant to antibiotics. A study investigated the use of Raman spectroscopy to monitor MRSA biofilm formation and found that Eugenol, when applied within the first 5 hours of biofilm formation, showed promise in breaking down the biofilm. The study also demonstrated the efficacy of Raman spectroscopy coupled with principal component analysis (PCA) in identifying planktonic bacteria from biofilm conglomerates.
Article
Chemistry, Organic
Hongic Won, Min-Gyung Son, Pisey Pel, Piseth Nhoek, Chae-Yeong An, Young-Mi Kim, Hee-Sung Chae, Young-Won Chin
Summary: Six new flavanones and fourteen known compounds were isolated from the methanol extract of Morus alba root bark. Betulinic acid (13) showed potent regulatory activity on PCSK9 and LDLR at both mRNA and protein levels, and it also inhibited PCSK9 secretion, indicating its potential as a future PCSK9 synthesis inhibitor.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Seung Yon Han, Jeong-Eun Yu, Byoung Hoon You, Seo-Yeon Kim, Mingoo Bae, Hee-Sung Chae, Young-Won Chin, Soo-Hwa Hong, Ju-Hee Lee, Seung Hyun Jung, Young Hee Choi
Summary: In a mouse model, the combination of trastuzumab and H9 extract for breast cancer treatment showed no changes in trastuzumab pharmacokinetics, indicating that H9 does not affect the exposure of trastuzumab.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)