Article
Zoology
Ting Xu, Rongxiang Tan, Yutao Zhu, Jian Ye
Summary: This study developed a new real-time PCR method for the detection of decapod iridescent virus 1 (DIV1) DNA. The method showed good linearity, repeatability, and specificity, and efficiently, sensitively, and reliably detected DIV1.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2023)
Article
Virology
Yanlin You, Limei Xu, Fang Li, Feng Yang
Summary: This study analyzed the structural proteins of Decapod iridescent virus 1 (DIV1) using mass spectrometry. The researchers purified DIV1 virions from infected Cherax quadricarinatus and identified 30 virus-encoded proteins. The findings provide a foundation for further investigation into viral infection and replication.
Article
Fisheries
Xiao-Meng Guo, Liang Qiu, Wen Gao, Guo-Hao Wang, Xing Chen, Jie Huang
Summary: This study evaluated the potential of thermal therapy to treat the newly found virus DIV1 in white leg shrimp. It found that thermal treatment at 36 degrees Celsius can eliminate the virus, providing a new treatment approach for shrimp varieties with special genetic properties.
Article
Environmental Sciences
Liang Qiu, Xiao-Meng Guo, Yong-Hui Feng, Jing-Yi Xing, Xian-Yun Ren, Jie Huang
Summary: Infection with iDIV1 is deemed as an emerging disease that affects shrimps and crabs, and it has gained attention from NACA and WOAH. China has categorized iDIV1 as a Class II animal pandemic disease. A study conducted on Penaeus japonicus demonstrated that the shrimp is susceptible to DIV1, showing clinical signs and a high mortality rate. These findings provide valuable support for the development of preventive and control strategies for DIV1.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Fisheries
Melony J. Sellars, Louise Franz, Ralf Joachim Moser
Summary: A set of new real-time PCR assays have been developed to detect DIV1 virus in shrimp tissues. These assays show high sensitivity and specificity, and demonstrate excellent diagnostic capability in clinical Litopenaeus vannamei samples. This molecular toolset will enhance global monitoring of DIV1 spread and serve as an early warning system.
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2023)
Article
Fisheries
Liang Qiu, Xing Chen, Wen Gao, Xiao-Meng Guo, Guo-Si Xie, Miao Gong, Bing Yang, Chen Li, Qing-Li Zhang, Jie Huang
Summary: The study investigated the infection parameters and symptoms of the economic crab species, swimming crab Po. trituberculatus, with DIV1, identifying it as a susceptible host for DIV1 infection. The presence of DIV1 in different tissues of diseased swimming crabs was confirmed, and potential transmission routes of DIV1 were preliminarily discussed.
Article
Biochemical Research Methods
Guixiang Tong, Weili Yin, Xiangqing Wu, Yong Lin, Guanghua Huang, Xiuli Chen, Xiaoyu Chen, Luanyu Huang, Tao Sun, Xinxian Wei, Xiaozheng Li
Summary: The new RPA assay for DIV1 showed high specificity and sensitivity, with a detection limit of 200 copies/50 μL, making it more sensitive than conventional PCR methods. In clinical experiments, the RPA method was able to effectively detect weak-positive samples.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Review
Fisheries
Xuzheng Liao, Jianguo He, Chaozheng Li
Summary: Decapod iridescent virus 1 (DIV1) is an emerging viral pathogen that poses a serious threat to crustacean farming. Research progress has been made in understanding its classification, host range, pathogenicity, and host immune response, providing insights for control and prevention strategies.
REVIEWS IN AQUACULTURE
(2022)
Article
Fisheries
Jinlin Chen, Wei Wang, Qin Zheng, Yunying Cheng, Qian Zhang, Shenfei Jiang, Jianming Chen
Summary: In this study, DIV1 detection methods were developed using a variable antigen receptor (vNAR) derived from shark heavy-chain antibodies. The lead vNAR, D02, was identified to target the viral antigen ORF064L. Indirect ELISA and dot-blot assays were developed based on D02 vNAR, demonstrating high sensitivity and specificity compared to qRT-PCR. This is the first study to develop a vNAR against DIV1, providing a robust tool for its diagnosis.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Jiraporn Srisala, Piyachat Sanguanrut, Dararat Thaiue, Saensook Laiphrom, Jittima Siriwattano, Juthatip Khudet, Sorawit Powtongsook, Timothy W. Flegel, Kallaya Sritunyalucksana
Summary: IMNV and DIV1 are lethal viruses for shrimp, first discovered in 2004 and 2016 respectively, with 99-100% sequence identity in positive tests on captured P. monodon. The study highlights the potential risk of introducing these viruses to shrimp hatcheries and farms through apparently healthy captured P. monodon and recommends surveillance and precautions be taken.
Article
Fisheries
Liang Qiu, Xiao-Meng Guo, Guo-Si Xie, Yong-Hui Feng, Jing-Yi Xing, Chen Li, Bing Yang, Jie Huang
Summary: This study reported a severe case of natural infection with DIV1 virus in cultured swimming crab in a farm in Shandong Province of China. The infected crabs showed symptoms of slow movement, loss of appetite, and empty stomachs and guts. Histopathological examination revealed eosinophilic inclusions and pyknosis in the hemocytes of the crab gill tissue. Transmission electron microscopy confirmed the presence of DIV1 particles, and further analysis showed significantly longer coagulation time in the diseased crabs compared to healthy individuals. This study highlights the risk of DIV1 to the swimming crab farming industry and provides a new approach for on-site diagnosis.
Article
Fisheries
Yanlin You, Feng Yang, Fang Li
Summary: Decapod iridescent virus 1 (DIV1) infection and replication were studied in primary cultured hematopoietic tissue (HPT) cells of crayfish Cherax quadricarinatus. DIV1 was found to infect and express viral genes in the cultured cells through RT-PCR and Western blot analysis. Immunofluorescent analysis showed an infection rate of around 23%. Viral DNA copy numbers increased in both the cells and culture medium as the infection progressed. Transmission electron microscopy analysis and animal challenging experiments confirmed the production and release of infectious progeny virions. In conclusion, the primary culture of C. quadricarinatus HPT cells can support the infection and proliferation of DIV1, making it a suitable in vitro system for studying the virus.
Article
Microbiology
Minze Liao, Xuzheng Liao, Xinxin Long, Jichen Zhao, Zihao He, Jingyue Zhang, Tingfen Wu, Chengbo Sun
Summary: This study found that Decapod iridescent virus 1 (DIV1) can cause changes in the shrimp immune system and intestinal microbiota, increasing the risk of secondary bacterial infections in shrimp. In addition, some metabolic pathways are related to shrimp immunity and may play a role in resisting DIV1 infection. Therefore, this study is of great significance for a better understanding of the interactions between shrimp immunity and intestinal microbiota.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Yiping Lu, Chien TU, Chiehhao Wu, Iwen Chen, Mingchu Cheng
Summary: This study aimed to identify and characterize Decapod iridescent virus 1 (DIV1) in shrimp farms in northern Taiwan in 2020. DIV1 was identified as the causative agent of the outbreaks and was found to infect cultured shrimp through natural routes of infection, causing varying mortality rates. Histopathology, electron microscopy, and phylogenetic analysis supported the identification of DIV1 as the outbreak's pathogen.
VETERINARY MEDICINE AND SCIENCE
(2023)
Article
Microbiology
Zihao He, Jichen Zhao, Xieyan Chen, Minze Liao, Yuan Xue, Jianing Zhou, Haozhen Chen, Guoliang Chen, Shuang Zhang, Chengbo Sun
Summary: The study revealed the toxicity of DIV1 to Marsupenaeus japonicus and its inhibition of enzyme activities. Transcriptomic analysis showed the genomic changes caused by DIV1 infection, as well as the significant impact on pathways and signaling pathways related to virus invasion, replication, and host antiviral infection.
FRONTIERS IN MICROBIOLOGY
(2021)
