Mechanism of Pb absorption in wheat grains

Atmospheric deposition is the primary source of external environmental media for lead (Pb) influx in wheat grains. However, the mechanisms of Pb grain absorption remains unclear. We explored this mechanism through comparative experiments, involving defoliating leaf blades (TG) and a control group (CK) of field wheat after the anthesis stage. Scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy analysis displayed that leaves and ears can directly absorb atmospheric deposition Pb through stomata. Compared with CK, the yield, grain Pb content, and grain Pb accumulation of TG wheat were significantly decreased by 13.25%, 22.10%, and 32.58%, respectively. Combined with the Pb isotope analysis, the ear had the highest contribution to grain Pb followed by leaf and root. Simultaneously, the absorption rate of grain Pb demonstrated a dynamic trend of N shape. Dominant contribution periods of the root, leaf, and ear organs to grain Pb accumulation were different. Unlike the root system, the contribution of the aboveground to grain Pb increased gradually, and the contribution of leaf and ear to grain Pb were mainly concentrated in the early and late filling stage, respectively. Our findings can provide a theoretical basis for the control of Pb pollution in grains.

Automated summary

The study found that wheat leaves and ears can directly absorb atmospheric deposition lead through stomata, and wheat with defoliated leaves showed significantly reduced lead absorption. Lead isotope analysis showed that ears contributed the most to grain lead, followed by leaves and roots. Different organs had different dominant contribution periods to grain lead accumulation, with leaves and ears contributing mainly in the early and late filling stages.