Article
Nanoscience & Nanotechnology
Xiaotong Zhao, Dawei Ma, Guoyuan Ju, Bo Gui, Zhangyu Yao, Jia Zhang, Yanbin Zhao, Fangzhou Liu, Xiao Zhang
Summary: This study reveals a new mechanism of cisplatin resistance related to energy metabolism, showing enhanced synthesis of key enzymes, particularly cytochrome oxidase (CCO), in drug-resistant cancer cells. Inhibiting CCO activity can reverse drug resistance in cancer cells.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2023)
Article
Plant Sciences
Rosario Pantaleno, Denise Scuffi, Alex Costa, Elina Welchen, Roberta Torregrossa, Matthew Whiteman, Carlos Garcia-Mata
Summary: Hydrogen sulfide (H2S) is a gaseous signaling molecule that plays a role in physiological processes in plants, including regulating stomatal pore width. In this study, it was found that a mitochondrial-targeted H2S donor called AP39 can induce stomatal closure in a dose-dependent manner. The activity of mitochondrial cytochrome c (CYTc) and/or complex IV was shown to be necessary for AP39-dependent stomatal closure. AP39 also affected the mitochondrial inner potential, cytosolic ATP, hydrogen peroxide levels, and the oxidation of the glutathione pool in guard cells. These findings highlight the interplay between mitochondrial H2S, mitochondrial activity, and stomatal closure.
Article
Psychiatry
Johan Fernstrom, Synthia H. Mellon, Marlon A. McGill, Martin Picard, Victor Reus, Christina M. Hough, Jue Lin, Elissa S. Epel, Owen M. Wolkowitz, Daniel Lindqvist
Summary: The study found that mitochondrial markers did not differentiate between individuals with major depressive disorder and healthy controls, but could distinguish between SSRI responders and nonresponders. Mitochondrial dysfunction may be implicated in treatment response to SSRI in MDD.
TRANSLATIONAL PSYCHIATRY
(2021)
Article
Medicine, Research & Experimental
Bei Shi Lee, Kiel Hards, Curtis A. Engelhart, Erik J. Hasenoehrl, Nitin P. Kalia, Jared S. Mackenzie, Ekaterina Sviriaeva, Shi Min Sherilyn Chong, Malathy Sony S. Manimekalai, Vanessa H. Koh, John Chan, Jiayong Xu, Sylvie Alonso, Marvin J. Miller, Adrie J. C. Steyn, Gerhard Gruber, Dirk Schnappinger, Michael Berney, Gregory M. Cook, Garrett C. Moraski, Kevin Pethe
Summary: The study highlights the value of a cytochrome bd oxidase inhibitor in a drug combination targeting oxidative phosphorylation for tuberculosis treatment. When combined with Q203, it can enhance efficacy and kill replicating and antibiotic-tolerant mycobacteria.
EMBO MOLECULAR MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Zhen Li, Huijing Xia, Thomas E. Sharp, Kyle B. LaPenna, John W. Elrod, Kevin M. Casin, Ken Liu, John W. Calvert, Vinh Q. Chau, Fadi N. Salloum, Shi Xu, Ming Xian, Noriyuki Nagahara, Traci T. Goodchild, David J. Lefer
Summary: This study reveals that the reduction of 3-MST in the myocardium is associated with heart failure. Knockout of 3-MST worsens cardiac and vascular dysfunction in a mouse model of pressure overload-induced heart failure. Restoring branched-chain amino acid metabolism and administrating an H2S donor can alleviate the detrimental effects of 3-MST deficiency in heart failure.
CIRCULATION RESEARCH
(2022)
Article
Multidisciplinary Sciences
Sibiao Yue, Lei Wang, George N. DeMartino, FangZhou Zhao, Yi Liu, Matthew H. Sieber
Summary: The regulation of cellular proteostasis is connected to the metabolic state of quiescent cells in vivo. The authors demonstrate that shifts in the ubiquitin-proteome system are linked to mitochondrial metabolic changes and respiratory quiescence. Cellular proteostasis defects and mitochondrial dysfunction play a role in infertility, cancer, and age-related diseases. Through a multi-organism approach, it is shown that dynamic shifts in the ubiquitin proteasome system drive mitochondrial remodeling during cellular quiescence.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Matej L'uptak, Zdenek Fisar, Jana Hroudova
Summary: Mitochondrial dysfunction is involved in psychiatric and neurodegenerative disorders and can be used to predict treatment responsiveness. Understanding the mitochondrial effects of antidepressants is important to connect mitochondria with therapeutic and adverse effects.
Article
Agriculture, Dairy & Animal Science
Victoria Favorit, Wendy R. Hood, Andreas N. Kavazis, Patricia Villamediana, Kang Nian Yap, Hailey A. Parry, Amy L. Skibiel
Summary: Lactation requires increased nutrient and energy usage. Mitochondrial efficiency and biogenesis in the liver are positively associated with milk yield, while skeletal muscle mitochondrial efficiency remains consistent but experiences oxidative damage.
Review
Ecology
Rebecca E. Koch, Katherine L. Buchanan, Stefania Casagrande, Ondi Crino, Damian K. Dowling, Geoffrey E. Hill, Wendy R. Hood, Matthew McKenzie, Mylene M. Mariette, Daniel W. A. Noble, Alexandra Pavlova, Frank Seebacher, Paul Sunnucks, Eve Udino, Craig R. White, Karine Salin, Antoine Stier
Summary: The critical role that energy turnover plays in understanding variation in performance and fitness among individuals has long been recognized by biologists. While whole-organism metabolic studies have provided key insights into ecological and evolutionary processes, constraints at subcellular levels, such as within mitochondria, can also optimize metabolism. This exploration of mitochondrial aerobic metabolism's influence on organismal performance highlights important areas for future research in understanding ecological and evolutionary processes.
TRENDS IN ECOLOGY & EVOLUTION
(2021)
Review
Cell Biology
Pierre Eyenga, Benjamin Rey, Lilia Eyenga, Shey-Shing Sheu
Summary: The link between liver dysfunction and decreased mitochondrial oxidative phosphorylation in sepsis has been established. The efficiency of mitochondrial coupling collapses in the early stage but is expected to increase during recovery phases. Slipping reactions at the cytochrome oxidase and ATP synthase appear to be a determining element in regulating coupling efficiency.
Article
Chemistry, Multidisciplinary
Wenshu Cong, Li Meng, Yuanjie Pan, Han Wang, Jiahui Zhu, Yuanyu Huang, Qingli Huang
Summary: Population aging is a global issue, and this study presents a novel approach using Pd-based single-atom nanozymes to improve mitochondrial function and attenuate aging. The nanozyme mimics enzyme activities in the mitochondrial respiratory chain and enhances mitochondrial activities and abundance. Additionally, it demonstrates excellent anti-aging and neuroprotective effects through the activation of the mitochondrial unfolded protein response (UPRmt) pathway.
Article
Multidisciplinary Sciences
Liselotte Bruun Christiansen, Tine Lovso Dohlmann, Trine Pagh Ludvigsen, Ewa Parfieniuk, Michal Ciborowski, Lukasz Szczerbinski, Adam Kretowski, Claus Desler, Luca Tiano, Patrick Orlando, Torben Martinussen, Lisbeth Hoier Olsen, Steen Larsen
Summary: The study investigated the tissue-specific effects of chronic statin therapy on mitochondrial function in liver, heart, and skeletal muscle. The results suggest that statins may reduce mitochondrial respiratory capacity in the liver while having opposite effects on the heart and skeletal muscle.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Valeria Calabro, Mariana Garces, Lourdes Caceres, Natalia D. Magnani, Timoteo Marchini, Agustina Freire, Tamara Vico, Manuela Martinefski, Virginia Vanasco, Valeria Tripodi, Alejandro Berra, Silvia Alvarez, Pablo Evelson
Summary: Studies have shown that exposure to urban air pollution can lead to oxidative stress, mitochondrial dysfunction, increased oxidant production, and activation of antioxidant systems in the brain cortex of mice, suggesting a key role of oxidative stress and impaired mitochondrial function in CNS damage mechanisms triggered by air pollution.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Review
Plant Sciences
Isabelle Faria Matos, Luis Miguel Mazorra Morales, Diederson Bortolini Santana, Glaucia Michelle Cosme Silva, Mara Menezes de Assis Gomes, Ricardo Antonio Ayub, Jose Helio Costa, Jurandi Goncalves de Oliveira
Summary: Vitamin C is essential for metabolism, and humans rely on plants as a source of vitamin C. The last step of the vitamin C synthesis pathway in plants is linked to the respiratory chain of mitochondria and catalyzed by l-galactono-1,4-lactone dehydrogenase (l-GalLDH). This enzyme can introduce electrons into the respiratory chain and bypass the phosphorylating Complex III. This review explores the potential changes in respiratory function and physiological alterations related to low vitamin C in plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Olivier Lurette, Hala Guedouari, Jordan L. Morris, Rebeca Martin-Jimenez, Julie-Pier Robichaud, Genevieve Hamel-Cote, Mehtab Khan, Nicholas Dauphinee, Nicolas Pichaud, Julien Prudent, Etienne Hebert-Chatelain
Summary: The architecture of mitochondria is regulated by the tyrosine kinase Src, which affects mitochondrial morphology independently of mitochondrial size, cellular respiration, or ATP levels. This study highlights a novel function for Src in the control of mitochondrial dynamics.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Nicolas Pichaud, Andreas Ekstrom, Sophie Breton, Fredrik Sundstrom, Piotr Rowinski, Pierre U. Blier, Erik Sandblom
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Chloe Simard, Andrea Lebel, Eric Pierre Allain, Mohamed Touaibia, Etienne Hebert-Chatelain, Nicolas Pichaud
Article
Biochemistry & Molecular Biology
Chloe J. Simard, Mohamed Touaibia, Eric Pierre Allain, Etienne Hebert-Chatelain, Nicolas Pichaud
Article
Cell Biology
Hala Guedouari, Yasmine Ould Amer, Nicolas Pichaud, Etienne Hebert-Chatelain
Summary: Using in vitro proximity-dependent biotin identification, 51 candidate proteins that may interact with c-Src within the mitochondrial matrix were identified, with potential involvement in various mitochondrial functions. Additionally, 24 tyrosine phosphorylation sites in 17 mitochondrial proteins were identified as potential substrates for intramitochondrial Src. The study suggests that intramitochondrial Src can target multiple proteins and regulate diverse mitochondrial functions.
Article
Biochemistry & Molecular Biology
Robert J. Cormier, Rebekah Strang, Hichem Menail, Mohamed Touaibia, Nicolas Pichaud
Summary: Following a 10-day exposure to a high-fat diet, adult Drosophila melanogaster showed decreased mitochondrial respiration rates and increased contributions of non-classical providers to the electron transport system. Moreover, an accumulation of metabolites from various metabolic pathways and a drastic shift in lipid profile were observed. These results demonstrate the various responses of Drosophila melanogaster to high-fat diet treatment, indicating the development of metabolic inflexibility.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Biology
Lisa Bjerregaard Jorgensen, Johannes Overgaard, Florence Hunter-Manseau, Nicolas Pichaud
Summary: The heat tolerance of ectotherms, such as insects, is linked to the failure of oxidative phosphorylation (OXPHOS) and/or ATP production at critically high temperatures, with complex I-supported respiration (CI-OXPHOS) decreasing significantly at higher temperatures but the enzymatic catalytic capacity of complex I remaining intact. Despite the reduction in CI-OXPHOS, all species were able to maintain maximal OXPHOS capacity through oxidation of alternative substrates, indicating important mitochondrial flexibility at temperatures exceeding organismal heat limits. Interestingly, the failure of CI-OXPHOS and compensatory oxidation of alternative substrates occurred at temperatures correlated with species heat tolerance, suggesting a potential link between mitochondrial function and thermal tolerance in ectotherms.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Biology
Georges Hraoui, Sophie Breton, Gilles Miron, Luc H. Boudreau, Florence Hunter-Manseau, Nicolas Pichaud
Summary: This study investigated the impact of intermittent heat stress on the mitochondrial functions of the eastern oyster, revealing that oysters acclimated to 22.5 degrees C struggled more with heat shocks. However, both acclimated populations showed signs of potential recovery after the second heat shock.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Mohamed Touaibia, Patrick-Denis St-Coeur, Patrick Duff, Diene Codou Faye, Nicolas Pichaud
Summary: A series of thiazolidinediones were synthesized and studied for their effects on Drosophila melanogaster mitochondrial respiration. Compounds like 5-benzylidenethiazolidine-2,4-diones exhibited strong inhibitory capacity compared to other analogs, showing potential as potent mitochondrial pyruvate carrier inhibitors similar to UK5099. An analog, 5-benzylidenethiazolidine-2,4-dione 3, demonstrated positive effects on fruit fly survival and longevity, reversing the effects of a high fat diet and suggesting in vivo inhibition of MPC at the mitochondrial level.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Olivier Lurette, Hala Guedouari, Jordan L. Morris, Rebeca Martin-Jimenez, Julie-Pier Robichaud, Genevieve Hamel-Cote, Mehtab Khan, Nicholas Dauphinee, Nicolas Pichaud, Julien Prudent, Etienne Hebert-Chatelain
Summary: The architecture of mitochondria is regulated by the tyrosine kinase Src, which affects mitochondrial morphology independently of mitochondrial size, cellular respiration, or ATP levels. This study highlights a novel function for Src in the control of mitochondrial dynamics.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Anu S. Nath, Brendon D. Parsons, Stephanie Makdissi, Rebecca L. Chilvers, Yizhu Mu, Ceileigh M. Weaver, Irene Euodia, Katherine A. Fitze, Juyang Long, Michal Scur, Duncan P. Mackenzie, Andrew P. Makrigiannis, Nicolas Pichaud, Luc H. Boudreau, Andrew J. Simmonds, Christine A. Webber, Beata Derfalvi, Yannick Hammon, Richard A. Rachubinski, Francesca Di Cara
Summary: Peroxisomes play key roles in lipid metabolism and immune function, particularly in macrophage activation and cytoskeletal remodeling. Loss of peroxisome function can hinder host survival after infection.
Article
Physiology
Hichem A. Menail, Simon B. Cormier, Mariem Ben Youssef, Lisa Bjerregaard Jorgensen, Jess L. Vickruck, Pier Morin, Luc H. Boudreau, Nicolas Pichaud
Summary: This study investigated the responses of mitochondrial oxidative phosphorylation (OXPHOS) to temperature in three flying insects: honeybees, fruit flies, and potato beetles. The results showed that honeybees exhibit greater resilience to high temperatures compared to fruit flies, indicating a species-specific mitochondrial flexibility to sustain high oxygen consumption levels.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biology
Simon B. Cormier, Adele Leger, Luc H. Boudreau, Nicolas Pichaud
Summary: This study reveals previously unknown cellular mechanisms between summer and winter honeybees, showing differences in metabolism and immune system, with increased mitochondrial oxygen consumption and antibacterial response in winter bees.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Article
Biology
Erik Sandblom, Lucas A. Zena, Nicolas Pichaud
Summary: Acute warming increases the metabolism and heart rate of fish, while thermal acclimation may decrease heart rate through changes in intrinsic pacemaker rate and inhibitory vagal tone. This study found that rainbow trout exhibited a slightly higher heart rate and a trend of elevated vagal tone after acute warming, but routine heart rate was not affected by warm acclimation. Additionally, acute warming increased baroreflex sensitivity to reduced blood pressure, while chronic warming restored baroreflex sensitivity to normal levels.
JOURNAL OF THERMAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Robert J. Cormier, Jeremie A. Doiron, Mohamed Touaibia, Marc E. Surette, Nicolas Pichaud
Summary: This study investigates the effects of high-fat diets on metabolic dynamics using the fruit fly model. The results show that fatty acids are utilized for both lipogenesis and fatty acid oxidation after 2 days of exposure to a high-fat diet. After 4 days, several metabolic pathways are significantly affected, and an accumulation of specific fatty acids is observed. After 9 days, there is a clear dysfunction of the metabolic system. Overall, this study highlights the relevance of the fruit fly model in studying metabolic disorders and emphasizes the importance of the duration of high-fat diet exposure in understanding the dynamics of fundamental metabolic mechanisms.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)