Article
Agriculture, Dairy & Animal Science
Qiang Ding, Miaohan Jin, Peter Kalds, Chunhua Meng, Huili Wang, Jifeng Zhong, Xiaolong Wang, Yulin Chen
Summary: This study identified differential miRNA expression patterns in extracellular vesicles (EVs) from goat follicular fluid and demonstrated their significance in regulating follicular development. The findings suggest that EVs play biofunctional roles during follicular development in goats, involving signaling pathways such as FoxO, MAPK, and PI3K-AKT. The study contributes to a better understanding of the role of EVs in follicular development.
Article
Biochemistry & Molecular Biology
Anise Asaadi, Nima Azari Dolatabad, Hadi Atashi, Annelies Raes, Petra Van Damme, Michael Hoelker, An Hendrix, Osvaldo Bogado Pascottini, Ann Van Soom, Mojtaba Kafi, Krishna Chaitanya Pavani
Summary: This study compared two methods for isolating EVs from bovine follicular fluid and ampullary oviduct fluid, and found that EVs isolated using OptiPrep density gradient ultracentrifugation showed benefits for embryo development and quality.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medical Laboratory Technology
Hamid Reza Nejabati, Leila Roshangar, Mohammad Nouri
Summary: The decrease in reproductive potential with aging is attributed to the gradual decline in quantity and quality of ovarian reserve, which increases the risk of miscarriage, pregnancy loss, low ovarian stimulation, and oocyte abnormalities. Changes in cellular composition of follicles have been shown to contribute to the reduced fertility potential of older women. Moreover, extracellular vesicles (EVs), especially non-coding RNAs like miRNAs, have been found to play a role in cellular communication and their composition has been documented to alter with age, suggesting their potential as predictors of oocyte quality.
CLINICA CHIMICA ACTA
(2023)
Article
Cell Biology
Ricardo C. Abreu, Cristiana Ramos, Clarissa Becher, Miguel Lino, Carlos Jesus, Paula A. da Costa Martins, Patricia A. T. Martins, Maria Joao Moreno, Hugo Fernandes, Lino Ferreira
Summary: The study demonstrates that Exo-Fect is the most efficient method for modulating sEVs, leading to over 1000-fold upregulation of the miRNA of interest and successful delivery of miR-155-5p into target cells, demonstrating its functionality. Furthermore, the membrane alterations in Exo-Fect-loaded sEVs enhance internalization within target cells and decrease interaction with lysosomes.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Biochemistry & Molecular Biology
Anne-Sophie Neyroud, Regina Maria Chiechio, Gregory Moulin, Solene Ducarre, Claire Heichette, Aurelien Dupont, Mathieu Budzynski, Pascale Even-Hernandez, Maria Jose Lo Faro, Marina Yefimova, Valerie Marchi, Celia Ravel
Summary: The morphology and quantification of extracellular vesicles (EVs) in follicular fluid (FF) of healthy individuals were studied in this research. EVs play important roles in the development of female gametes, but limited knowledge exists regarding their morphology. The findings of this study provide a comprehensive morphological description and quantification of FF-derived EVs, serving as a reference for further research on EVs in ovarian diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Obstetrics & Gynecology
Zohar Barnett-Itzhaki, Sarah Knapp, Chaya Avraham, Catherine Racowsky, Russ Hauser, Valentina Bollati, Andrea A. Baccarelli, Ronit Machtinger
Summary: The study found that phthalate metabolite concentrations are associated with the expression of extracellular vesicle microRNAs (EV-miRNAs) in follicular fluid, potentially impacting pathways related to oocyte development, maturation, and fertilization.
HUMAN REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Maria Soares, Maria M. Pinto, Rui Jorge Nobre, Luis Pereira de Almeida, Maria da Graca Rasteiro, Teresa Almeida-Santos, Joao Ramalho-Santos, Ana Paula Sousa
Summary: This study compared the efficacy and purity of two methods for isolating extracellular vesicles (EVs): size-exclusion chromatography (SEC) and ultracentrifugation (UC). The results showed that UC yielded a significantly higher number of EVs, while SEC produced purer EVs.
Article
Obstetrics & Gynecology
Zhi Zhou, Yong Zhang, Xiaopo Zhang, Juan Zhang, Guohui Yi, Bangbei Wan, Yejuan Li, Hui Lu, Can Tan, Weiying Lu
Summary: Polycystic ovary syndrome (PCOS) is a complex condition in reproductive-age females, in which dysplasia of ovarian granulosa cells (GC) is involved. Follicular fluid (FF)-derived extracellular vesicles (Evs) play a crucial role in cell-to-cell communication during follicular development. This study explores the function and mechanism of FF-Evs in contributing to the viability and apoptosis of GC cells in PCOS development. It is found that FF-Evs can alleviate DHEA-induced GC damage by delivering LINC00092, a lncRNA that inhibits the binding of RNA binding protein LIN28B to pre-miR-18-5p and thereby promotes the expression of miR-18b-5p, a miRNA known to alleviate PCOS by suppressing PTEN mRNA.
REPRODUCTIVE SCIENCES
(2023)
Article
Reproductive Biology
Yunqi Zhao, Ying Wang, Zimeng Ling, Yaxing Xue, Deji Luan, Jian Kang, Yong Zhang, Fusheng Quan
Summary: Apoptosis of granulosa cells is crucial in mammalian follicular atresia and affects oocyte development. This study successfully isolated a subtype of small extracellular vesicles (sEVs), low-density small EVs (LD-sEVs), from bovine follicular fluid. The LD-sEVs were found to be taken up by granulosa cells and inhibit apoptosis, mainly through the let-7i miRNA component targeting FASLG. These findings highlight the important role of LD-sEVs and let-7i in regulating follicular granulosa cell apoptosis and follicular development.
Article
Biotechnology & Applied Microbiology
Peiwen Fu, Yumeng Guo, Yanan Luo, Michael Mak, Jianguo Zhang, Wenrong Xu, Hui Qian, Zhimin Tao
Summary: This study presents a nano-scale fluorescent off-on complex encapsulated by small extracellular vesicles (sEVs) for real-time visualization and evaluation of gene therapy efficiency in cancer. The results demonstrate that the complex is able to activate the turn-on fluorescent signal and exhibit the longest retention time in vivo, promoting cancer apoptosis both in vitro and in vivo.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Yifei Qin, Jing Zhang, Ricardo David Avellan-Llaguno, Xu Zhang, Qiansheng Huang
Summary: DEHP exposure promotes the secretion of miR-26a-5p in sEV, subsequently enhancing the EMT, migration, and invasion ability in neighboring normal cells.
ENVIRONMENTAL POLLUTION
(2021)
Article
Chemistry, Multidisciplinary
Yuan Yin, Bingxin Liu, Yulin Cao, Surui Yao, Yuhang Liu, Guoying Jin, Yan Qin, Ying Chen, Kaisa Cui, Leyuan Zhou, Zehua Bian, Bojian Fei, Shenglin Huang, Zhaohui Huang
Summary: This study reveals that colorectal cancer cells can induce macrophage polarization and PD-L1 expression through the release of small extracellular vesicles (sEVs), leading to suppressed CD8(+) T cell activity and increased tumor growth. The miR-21-5p and miR-200a derived from sEVs play key regulatory roles in this process. Inhibiting the secretion of specific sEV-miRNAs from CRC and targeting PD-L1 in TAMs may serve as novel methods for CRC treatment.
Review
Reproductive Biology
Yanshe Xie, Changhua Wu, Weidong Chen, Zhenfang Wu, Gengyuan Cai, Linjun Hong
Summary: Extracellular vesicles (EVs) are nanoscale lipid vesicles derived from cells that participate in cell-cell communication by delivering cargo such as mRNAs, proteins, and non-coding RNAs to recipient cells. MicroRNAs (miRNAs), typically 22 nucleotides long, play a crucial role in developmental and pathophysiological processes by regulating gene expression in recipient cells. Infertility is a global health issue, and the use of EVs-encapsulated miRNAs in assisted reproductive technology (ART) could potentially optimize ART, improve reproductive rate, and treat infertility. This review discusses the current understanding of EVs-encapsulated miRNAs in reproductive regulation.
Article
Biology
Mohammad Mehedi Hasan, Qurat Ul Ain Re, Freddy Lattekivi, Janeli Viil, Kasun Godakumara, Keerthie Dissanayake, Aneta Andronowska, Ulle Jaakma, Alireza Fazeli
Summary: Before the union of an egg and spermatozoon, multiple vital processes occur in the female reproductive system. The maturation of spermatozoa in the female reproductive tract is crucial for fertilization. Follicular fluid (FF) and its extracellular vesicles (EVs) play a significant role in supporting sperm functions, with specific effects based on species and source of FF EVs. This study demonstrates that bovine FF EVs can support sperm function and contribute to a favorable periconceptional microenvironment.
Review
Cell Biology
Wanran Li, Yun Zheng
Summary: Alzheimer's disease (AD) is a neurodegenerative disease that affects memory, language, and thinking. Over 55 million people were diagnosed with AD or other forms of dementia worldwide in 2020. MicroRNAs (miRNAs) have been found to play important roles in AD development and have potential applications in diagnosis and treatment. Extracellular vesicles (EVs) containing miRNAs are present in body fluids and are involved in cell-to-cell communication. This study summarizes the dysregulated miRNAs in EVs derived from different body fluids and brain tissues of AD patients, and discusses their potential functions and applications in AD. Certain miRNAs, such as miR-125b-5p and miR-9-5p, show promise as diagnostic markers and potential therapeutic targets for AD.
Article
Biochemistry & Molecular Biology
Huanhuan Chen, Lei Zhang, Zhenqiang Wang, Haoya Chang, Xiaogang Xie, Liangzheng Fu, Yong Zhang, Fusheng Quan
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2019)
Article
Biochemistry & Molecular Biology
Haoya Chang, Huanhuan Chen, Lei Zhang, Yile Wang, Xiaogang Xie, Yong Zhang, Fusheng Quan
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2019)
Article
Biochemistry & Molecular Biology
Kai Meng, Xiaomei Wang, Yuanyuan He, Hengqin Wang, Xiaogang Xie, Yong Zhang, Fusheng Quan
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2019)
Article
Agriculture, Dairy & Animal Science
X. Wang, K. Meng, Y. Wang, Y. Zhao, F. Lin, X. Liu, Y. Zhang, F. Quan
Summary: The Wilms' tumor gene WT1 has been found to regulate granulosa cell steroidogenesis in bovine, with its (-KTS) variant having a more significant effect on theca cells. This gene downregulation increases progesterone secretion in bovine theca cells but does not affect proliferation and apoptosis.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Yuanyuan He, Yile Wang, Hengde Zhang, Yong Zhang, Fusheng Quan
Summary: The addition of alpha-lipoic acid (alpha-LA) to the in vitro maturation medium significantly improved the maturation and development rates of goat oocytes and parthenogenetic embryos. This improvement may be attributed to the maintenance of total antioxidant capacity in the oocytes during the culture period.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Agriculture, Dairy & Animal Science
Wang Ying, Wang Hengqin, Wang Xiaomei, Zhao Yunqi, Zhang Yong, Quan Fusheng
Summary: The research found that 100μg/ml of extracellular vesicles can significantly promote the proliferation and hormone synthesis of bovine ovarian cortical stromal cells, which is of great significance for elucidating the cell-to-cell communication mechanisms in follicular fluid and ovarian development.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Multidisciplinary Sciences
Yuanyuan He, Kai Meng, Xiaomei Wang, Zhihang Dong, Yong Zhang, Fusheng Quan
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2020)