Article
Biochemistry & Molecular Biology
Keresztely Merkel, David Szollosi, Ildiko Horvath, Balint Jezso, Zsolt Baranyai, Krisztian Szigeti, Zoltan Varga, Imre Hegedus, Parasuraman Padmanabhan, Balazs Gulyas, Ralf Bergmann, Domokos Mathe
Summary: This study reports a new method for labeling platelets using the peptide duramycin, allowing for efficient tracking of platelet biodistribution in vivo using single photon emission tomography (SPECT), and obtaining quantitative biodistribution images.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Olga Bragina, Vladimir Chernov, Mariia Larkina, Anstasiya Rybina, Roman Zelchan, Eugeniy Garbukov, Maryam Oroujeni, Annika Loftenius, Anna Orlova, Jens Soerensen, Fredrik Y. Frejd, Vladimir Tolmachev
Summary: The determination of tumor HER2 status is crucial for HER2-targeted treatments. This study evaluated the safety and dosimetry of Tc-99m-ZHER2:41071 Affibody molecule for SPECT/CT imaging of HER2 expression. The results showed that the injection of Tc-99m-ZHER2:41071 was safe and provided effective discrimination between HER2-positive and HER2-low/negative tumors.
Article
Medicine, Research & Experimental
Jiyun Shi, Shuaifan Du, Rongxi Wang, Hannan Gao, Qi Luo, Guozhu Hou, Yidong Zhou, Zhaohui Zhu, Fan Wang
Summary: HER2-specific imaging can accurately determine HER2 expression in breast cancer patients, and Tc-99m-HP-Ark2 SPECT/CT imaging shows a significant linear correlation with tumor HER2 expression. In a pilot clinical study, Tc-99m-HP-Ark2 SPECT/CT imaging demonstrated high sensitivity (89.5%) and specificity (88.2%) in reflecting HER2 status.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Guangxing Yin, Qing Ruan, Yuhao Jiang, Junbo Zhang
Summary: The study focuses on the development of novel radiolabeled amino acid tumor imaging agents and evaluates the in vitro stability, partition coefficient, and biodistribution of two Tc-99m-labeled complexes. The results demonstrate that [Tc-99m]TcO-MOPADTC shows higher tumor uptake, tumor/muscle ratio, and tumor retention compared to [Tc-99m]TcN-MOPADTC. The competitive inhibition assay provides insights into the cellular entry mechanism of [Tc-99m]TcO-MOPADTC.
Article
Radiology, Nuclear Medicine & Medical Imaging
Olga Bragina, Vladimir Chernov, Alexey Schulga, Elena Konovalova, Eugeniy Garbukov, Anzhelika Vorobyeva, Anna Orlova, Liubov Tashireva, Jens Sorensen, Roman Zelchan, Anna Medvedeva, Sergey Deyev, Vladimir Tolmachev
Summary: The study evaluated the safety, biodistribution, and dosimetry of Tc-99m-(HE)(3)-G3 in breast cancer patients, showing that the imaging method provides a low absorbed dose burden on patients and is safe and well-tolerated.
JOURNAL OF NUCLEAR MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Adamantia Apostolopoulou, Aristeidis Chiotellis, Evangelia-Alexandra Salvanou, Konstantina Makrypidi, Charalampos Tsoukalas, Fotis Kapiris, Maria Paravatou-Petsotas, Minas Papadopoulos, Ioannis C. Pirmettis, Przemyslaw Kozminski, Penelope Bouziotis
Summary: This study successfully developed four new Au nanoconjugates radiolabeled with technetium-99m via thiol-bearing ligands attached to the NP surface. The functionalized AuNPs showed excellent in vitro stability and high radiochemical purity, demonstrating their potential application in multimodality imaging or in vivo tracking of drug-carrying AuNPs.
Article
Radiology, Nuclear Medicine & Medical Imaging
Tianyu Liu, Yue Wu, Linqing Shi, Liqiang Li, Biao Hu, Yanpu Wang, Hannan Gao, Xiaolu Yu, Xin Zhang, Huiyun Zhao, Yakun Wan, Bing Jia, Fan Wang
Summary: In this study, a site-specifically labeled EpCAM-targeted nanobody was synthesized and evaluated for the noninvasive imaging of EpCAM expression using SPECT/CT. The results showed high EpCAM specificity and the potential for clinical translation.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Chemistry, Medicinal
Yuhao Jiang, Si'an Fang, Xuran Zhang, Junhong Feng, Qing Ruan, Junbo Zhang
Summary: The 99mTc-labeled deferoxamine radiotracer showed high radiochemical purity and good in vitro stability, with specific binding to bacteria. In mice study, it demonstrated higher uptake in bacterial infection tissues compared to sterile inflammation, suggesting its potential as a bacterial infection imaging radiotracer.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Medicine, Research & Experimental
Qing Ruan, Junhong Feng, Yuhao Jiang, Xuran Zhang, Xiaojiang Duan, Qianna Wang, Guangxing Yin, Di Xiao, Junbo Zhang
Summary: Fibroblast activation protein (FAP) is highly expressed in cancer-associated fibroblasts (CAFs) and has become a promising target for tumor therapy. Two isocyanide-containing FAP inhibitors were synthesized, radiolabeled, and showed good stability and specificity to FAP. These inhibitors demonstrated high target affinity and promising tumor imaging potential in preclinical studies.
MOLECULAR PHARMACEUTICS
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Mark Ooms, Dirk von Mallek, Hans-Juergen Kaiser, Frank Hoelzle, Felix M. Mottaghy, Ali Modabber
Summary: This study compared the performance of three different tracers in Sentinel Lymph Node (SLN) detection in cutaneous malignancies of the head. The results showed that TcTM had comparable overall performance to TcSC and TcAC.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Mariam Jaber, Jila Taherpour, Berthold Voges, Ivayla Apostolova, Thomas Sauvigny, Patrick M. House, Michael Lanz, Matthias Lindenau, Susanne Klutmann, Tobias Martens, Stefan Stodieck, Ralph Buchert
Summary: The study compared the performance of two tracers in ictal brain perfusion SPECT and found no significant difference in identifying the seizure onset zone (SOZ) between Tc-99m-HMPAO and Tc-99m-ECD. The results did not provide evidence to favor one tracer over the other for this purpose.
CLINICAL NUCLEAR MEDICINE
(2021)
Article
Chemistry, Medicinal
Paola Vallejo-Armenta, Guillermina Ferro-Flores, Clara Santos-Cuevas, Francisco Osvaldo Garcia-Perez, Pamela Casanova-Trivino, Bayron Sandoval-Bonilla, Blanca Ocampo-Garcia, Erika Azorin-Vega, Myrna Luna-Gutierrez
Summary: This study evaluated the application of [Tc-99m]Tc-iFAP for tumor stroma imaging in six different cancer types and analyzed them from the perspective of stromal heterogeneity. The results showed that [Tc-99m]Tc-iFAP has potential for prognostic assessment of some solid tumors, particularly breast cancer.
Article
Radiology, Nuclear Medicine & Medical Imaging
Zhonglin Lu, Gefei Chen, Han Jiang, Jingzhang Sun, Ko-Han Lin, Greta S. P. Mok
Summary: The study aimed to reduce the mismatch between static [Tc-99m]Tc-MAA SPECT and CT using two registration schemes, resulting in improved estimation of lung shunting fraction (LSF) and tumor-to-normal liver ratio (TNR). The results showed that registration significantly reduced estimation errors and improved dosimetric estimation.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
Editorial Material
Radiology, Nuclear Medicine & Medical Imaging
Selin Kesim, Salih Ozguven, Kevser Oksuzoglu, Tanju Yusuf Erdil
Summary: We report a pediatric case of T-cell lymphoblastic leukemia with persistent chylothorax who underwent lymphoscintigraphy to identify the site of leakage. Planar lymphoscintigraphy with SPECT/CT revealed hot spots at the left jugulovenous angle and the confluens of the vena brachiocephalica to vena cava, which were consistent with the sites of leakage. Hybrid imaging with SPECT/CT technique has a significant impact on accurately detecting lymphatic system defects in patients with chylothorax.
CLINICAL NUCLEAR MEDICINE
(2022)
Article
Chemistry, Medicinal
Maria Alejandra Asensio Ruiz, Angela Alonso Garcia, Maria de la Luz Bravo-Ferrer Moreno, Iria Cebreiros-Lopez, Jose Antonio Noguera-Velasco, Antonio Abel Lozano-Perez, Teresa Martinez Martinez
Summary: Nuclear imaging, using radiolabeled nanomaterials as imaging probes, is a valuable tool for medical diagnosis. This study developed a direct method for radiolabeling FITC-tagged silk fibroin nanoparticles (SFN) and demonstrated its stability and efficiency in biological media. The dual-labeling method showed potential for further investigations of SFN biodistribution in vivo.
Review
Spectroscopy
Erika Ponzini, Carlo Santambrogio, Antonella De Palma, Pierluigi Mauri, Silvia Tavazzi, Rita Grandori
Summary: This review focuses on the progress and methods of tear proteomics as a potential source of biomarkers, emphasizing on applications to biomarker discovery and critical steps of the experimental pipeline. Despite limited studies, tear quantitative proteomics offer unique contributions to personalized approaches in therapy and diagnosis.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Pharmacology & Pharmacy
Lucia Salvioni, Filippo Testa, Linda Barbieri, Marco Giustra, Jessica Armida Bertolini, Giulia Tomaino, Paolo Tortora, Davide Prosperi, Miriam Colombo
Summary: Ribosome-inactivating proteins, such as Saporin toxin, have been used as potential alternative cancer therapies due to their ability to inhibit protein synthesis. However, their limited cell uptake has hindered their application. In this study, Saporin was successfully immobilized on iron oxide nanoparticles and their cytotoxic effect on cancer cells was demonstrated by promoting their cellular uptake.
Article
Multidisciplinary Sciences
Miriam Colombo, Laura Marongiu, Francesca Mingozzi, Roberta Marzi, Clara Cigni, Fabio Alessandro Facchini, Rany Rotem, Mihai Valache, Giulia Stucchi, Giuseppe Rocca, Laura Gornati, Maria Antonietta Rizzuto, Lucia Salvioni, Ivan Zanoni, Alessandro Gori, Davide Prosper, Francesca Granucci
Summary: Calcineurin inhibitors can prevent transplant rejection but also hinder graft tolerance. Inhibiting calcineurin only in dendritic cells instead of T cells can prevent T cell responses while allowing for tolerance development. A new generation of selective immune suppressive agents based on nanoparticles shows promise for better control of transplant acceptance.
Article
Medical Laboratory Technology
Lucia Salvioni, Filippo Testa, Adela Sulejmani, Francesca Pepe, Pietro Giorgio Lovaglio, Paolo Berta, Roberto Dominici, Valerio Leoni, Davide Prosperi, Giorgio Vittadini, Miriam Colombo, Luisa Fiandra
Summary: SP-D can serve as a predictive biomarker for COVID-19 disease and its outcome. Elevated plasma levels of SP-D are associated with disease severity and mortality. Monitoring SP-D levels in hospitalized patients can guide therapeutic interventions.
CLINICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Matteo Monieri, Paolo Rainone, Angelina Sacchi, Alessandro Gori, Anna Maria Gasparri, Angela Coliva, Antonio Citro, Benedetta Ferrara, Martina Policardi, Silvia Valtorta, Arianna Pocaterra, Massimo Alfano, Dean Sheppard, Lorenzo Piemonti, Rosa Maria Moresco, Angelo Corti, Flavio Curnis
Summary: The alpha v[36- and alpha v[38-integrins, cell-adhesion receptors upregulated in tumors, can bind to the tumor-homing peptide 5a containing an RGDL motif, suggesting its potential as a target for tumor imaging and therapy.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Rheumatology
Eleonora Cantoni, Ivan Merelli, Davide Stefanoni, Alessandro Tomelleri, Corrado Campochiaro, Vito Giordano, Maddalena Panigada, Elena M. Baldissera, Laura Merlo Pich, Valentina Natoli, Athanasios Ziogas, Jorge Dominguez-Andres, Giacomo De Luca, Davide Mazza, Samuel Zambrano, Daniela Gnani, Marina Ferrarini, Elisabetta Ferrero, Alessandra Agresti, Barbara Vergani, Biagio Eugenio Leone, Simone Cenci, Angelo Ravelli, Marco Matucci-Cerinic, Angelo D'Alessandro, Leo A. B. Joosten, Lorenzo Dagna, Mihai G. Netea, Raffaella Molteni, Giulio Cavalli
Summary: This study investigated the role of trained immunity (TI) in the pathogenesis of giant cell arteritis (GCA). It was found that monocytes from GCA patients exhibited typical features of TI, including enhanced IL-6 production, immunometabolic changes, and epigenetic changes promoting pro-inflammatory activation. Immunometabolic changes of TI were also observed in myelomonocytic cells in GCA lesions and were required for enhanced cytokine production.
Article
Cell Biology
Lara Toffali, Beatrice D'Ulivo, Cinzia Giagulli, Alessio Montresor, Elena Zenaro, Massimo Delledonne, Marzia Rossato, Barbara Iadarola, Andrea Sbarbati, Paolo Bernardi, Gabriele Angelini, Barbara Rossi, Nicola Lopez, Wolfgang A. Linke, Andreas Unger, Dario Di Silvestre, Louise Benazzi, Antonella De Palma, Sara Motta, Gabriela Constantin, Pierluigi Mauri, Carlo Laudanna
Summary: Response to multiple microenvironmental cues and resilience to mechanical stress are essential features of trafficking leukocytes. Titin (TTN) plays an unexpected role in the regulation of mechanisms of lymphocyte trafficking. It governs the morphogenesis of plasma membrane microvilli, controls chemotaxis and integrin activation, and ensures T lymphocyte survival in the blood stream. LTTN1 is a critical and versatile housekeeping regulator of T lymphocyte trafficking.
Article
Oncology
Martina Del Nero, Alessandro Colombo, Stefania Garbujo, Chiara Baioni, Linda Barbieri, Metello Innocenti, Davide Prosperi, Miriam Colombo, Luisa Fiandra
Summary: The interaction between tumor cells and activated fibroblasts plays a critical role in the malignant features of desmoplastic carcinomas. Transforming growth factor beta (TGF-beta) and Platelet-Derived Growth Factor (PDGF) contribute to the pro-tumorigenic phenotypes in fibroblasts, while Interleukin-6 (IL-6) released by activated fibroblasts enhances tumor-cell invasiveness and chemoresistance. This study validates the use of advanced cell culture models to investigate the interplay between mammary tumor cells and fibroblasts, revealing the role of TGF-beta, PDGF, and IL-6 in breast cancer progression.
Article
Cell Biology
Davide Perico, Ying Tong, Lichao Chen, Shoji Imamichi, Yu Sanada, Masamichi Ishiai, Minoru Suzuki, Mitsuko Masutani, Pierluigi Mauri
Summary: Boron neutron capture therapy (BNCT) is a selective radiotherapy that uses nuclear reactions to induce cell death in cancer cells. Proteomic analysis of extracellular vesicles (EVs) treated with BNCT identified proteins associated with apoptosis, DNA repair, and inflammatory response. The results highlight the potential of proteomics in identifying biomarkers and understanding the mechanisms of BNCT.
Article
Biochemistry & Molecular Biology
Paolo Rainone, Silvia Valtorta, Chiara Villa, Sergio Todde, Massimiliano Cadamuro, Gloria Bertoli, Donatella Conconi, Marialuisa Lavitrano, Rosa Maria Moresco
Summary: Breast cancer is the most common cancer in women and the second leading cause of death. Breast cancer cells show a metabolic shift towards increased glycolysis, making it an interesting target for metabolic drugs such as metformin. Recent studies have found that the antihypertensive drug syrosingopine, when combined with metformin, has a synergistic effect on various cancers. However, there is a need to develop in vivo biomarkers that can detect early clinical response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Emanuela Colombo, Marco Di Dario, Ramesh Menon, Maria Maddalena Valente, Claudia Bassani, Nicole Sarno, Davide Mazza, Federico Montini, Lucia Moiola, Giancarlo Comi, Vittorio Martinelli, Cinthia Farina
Summary: Hepatocyte nuclear factor 4 a (HNF4a), an essential transcription factor for embryonic development, has been found to regulate the expression of inflammatory genes. Blocking HNF4a reduces immune activation and disease severity in multiple sclerosis (MS). Studies on human immune transcriptomes have identified HNF4a, SP1 and c-myc as master transcription factors regulating differential gene expression in MS. These transcription factors are activated by immune cell activation and regulated by environmental risk factors for MS. Targeting their expression or function may provide a potential therapeutic approach for MS and other inflammatory disorders.
JOURNAL OF AUTOIMMUNITY
(2023)
Article
Oncology
Saniya Arfin, Dhruv Kumar, Andrea Lomagno, Pietro Luigi Mauri, Dario Di Silvestre
Summary: This study reveals gene-miRNA interactions and topological biomarkers driving the development of head and neck squamous cell carcinoma (HNSCC) through comprehensive evaluation of gene and miRNA profiles. The molecular interactions activate genes promoting cell cycle and proliferation, and inactivate suppressive genes. Genes including VEGFA, EMP1, PPL, KRAS, MET, TP53, MMPs, and HOXs, and miRNAs including mir-6728 and mir-99a emerge as key players in HNSCC tumorigenesis.
Article
Oncology
Lorenzo Magrassi, Francesca Brambilla, Raffaello Vigano, Dario Di Silvestre, Louise Benazzi, Giuseppe Bellantoni, Gian Marco Danesino, Sergio Comincini, Pierluigi Mauri
Summary: Cystic formation in human primary brain tumors is a relatively rare event that varies depending on the histotype of the tumor. The composition of cystic fluid has been characterized in tumor resection samples, but there is no information on the evolution of cystic content. The proteome of cystic fluid obtained from different time points in the same tumor remained stable, and there are tumor-specific pathways that contribute to tumor-host interactions.
Article
Engineering, Biomedical
Francesca Donnaloja, Manuela Teresa Raimondi, Letizia Messa, Bianca Barzaghini, Federica Carnevali, Emanuele Colombo, Davide Mazza, Chiara Martinelli, Lucia Boeri, Federica Rey, Cristina Cereda, Roberto Osellame, Giulio Cerullo, Stephana Carelli, Monica Soncini, Emanuela Jacchetti
Summary: Mechanical stimuli from the extracellular environment affect cell morphology and functionality. The use of a custom-made 3D microscaffold, called the Nichoid, for cultivating mesenchymal stem cells (MSCs) has been shown to enhance the expression of stemness markers. This device appears to regulate gene activity by altering intracellular force transmission. The findings suggest that the Nichoid can influence MSC behavior and gene regulation through mechanical manipulation.
APL BIOENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Clara M. G. de Faria, Michael Bissoli, Riccardo Vago, Antonello E. Spinelli, Vincenzo Amendola, Miguel Gama
Summary: Nanomedicine utilizes nanomaterials for therapeutic and diagnostic purposes. This study investigates the cytotoxicity and tolerability of gold and gold-iron alloy nanoparticles, obtained through laser ablation in liquid, in different types of cells. The results provide important insights for the development of biocompatible and degradable nanomaterials.