Review
Biochemistry & Molecular Biology
Patricia Martins-Dias, Luisa Romao
Summary: Nonsense mutations can lead to dysfunctional proteins, but nonsense suppression therapy has the potential to restore protein function and treat a variety of genetic disorders. However, the efficiency of suppression may be influenced by nonsense-mediated decay, highlighting the importance of using NMD inhibitors or readthrough-compound potentiators to enhance therapeutic effects.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Venkateshwar Mutyam, Jyoti Sharma, Yao Li, Ning Peng, Jianguo Chen, Li Ping Tang, Emily Falk Libby, Ashvani K. Singh, Katja Conrath, Steven M. Rowe
Summary: Premature-termination codons (PTCs) in the CFTR gene lead to nonfunctional CFTR protein, accounting for 11% of CF-causing alleles with no current effective treatments. Novel CFTR correctors and potentiators show comparable effects to existing ones in vitro, and their combination can enhance the improvement of CFTR function with terminal PTC mutations.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Medicinal
Christie Morrill, Westley J. Friesen, Suresh Babu, Ramil Y. Baiazitov, Wu Du, Diane B. Karloff, Chang -Sun Lee, Young-Choon Moon, Hongyu Ren, Jairo Sierra, Yuki Tomizawa, Priya Vazirani, Ellen M. Welch, Xiaojiao Xue, Jin Zhuo
Summary: Using small molecules to induce readthrough is an effective method for treating genetic diseases and cancers. This study introduces a series of novel compounds that show potential for inducing readthrough in cells, either as combination therapy or standalone treatment.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Multidisciplinary Sciences
Laure Bidou, Olivier Bugaud, Goulven Merer, Matthieu Coupet, Isabelle Hatin, Egor Chirkin, Sabrina Karri, Stephane Demais, Pauline Francois, Jean-Christophe Cintrat, Olivier Namy
Summary: In this study, a new drug was developed and evaluated for its clinical potential in treating genetic diseases caused by premature termination codons (PTCs). The drug, TLN468, was found to be more effective than the currently used gentamicin and acted on a broader range of sequences without affecting normal stop codon readthrough.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Pharmacology & Pharmacy
Renata B. V. Abreu, Thiago T. Gomes, Thales C. Nepomuceno, Xueli Li, Mateus Fuchshuber-Moraes, Giuliana De Gregoriis, Guilherme Suarez-Kurtz, Alvaro N. A. Monteiro, Marcelo A. Carvalho
Summary: This study evaluates the readthrough of clinically relevant PTC variants in the breast and ovarian cancer-predisposing gene BRCA1 for the first time, demonstrating that the aminoglycoside G418 can induce PTC readthrough and restore full-length protein synthesis and function.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell Biology
Joseph J. Porter, Christina S. Heil, John D. Lueck
Summary: Nonsense mutations create defective truncated proteins by changing amino acid codons. While most PTC therapeutics focus on promoting PTC read-through, there is a need for agents that can recode PTCs with the correct amino acids.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2021)
Article
Medicine, Research & Experimental
Federica Corrao, Maria Grazia Zizzo, Marco Tutone, Raffaella Melfi, Ignazio Fiduccia, Pietro Salvatore Carollo, Aldo Di Leonardo, Gaetano Caldara, Riccardo Perriera, Andrea Pace, Beatrice Belmonte, Selene Sammataro, Ivana Pibiri, Laura Lentini
Summary: This study investigated the acute toxicological effects of three Translational Readthrough Inducing Drugs (TRIDs) on mice and found that these drugs showed good tolerability without causing significant adverse effects in the mice.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Daniel R. McHugh, Calvin U. Cotton, Craig A. Hodges
Summary: This study demonstrated synergy between NMD inhibitors and readthrough agents in increasing functional protein quantity following readthrough, suggesting a potential therapeutic option for treating nonsense mutations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mikel D. D. Ghelfi, Saleem Y. Y. Bhat, Hong Li, Barry S. S. Cooperman
Summary: The research findings show that Ataluren acts as a competitive inhibitor, blocking the catalytic function of the release factor complex (RFC) and inducing readthrough. This opens up the possibility of discovering new readthrough-inducing drugs that are both low in toxicity and more effective at stimulating readthrough.
Article
Chemistry, Medicinal
Nesrine Benslimane, Federica Miressi, Camille Loret, Laurence Richard, Angelique Nizou, Ioanna Pyromali, Pierre-Antoine Faye, Frederic Favreau, Fabrice Lejeune, Anne-Sophie Lia
Summary: Nonsense mutations play a role in peripheral neuropathies by causing premature termination codons at the mRNA level. Readthrough molecules or NMD inhibitors, such as amlexanox, could be potential therapies for hereditary neuropathies. In the study, treatment with amlexanox on patient-derived neuronal cells carrying a specific mutation resulted in stabilization of GDAP1 mRNAs and restoration of mitochondrial morphology, highlighting the potential of readthrough molecules and NMD inhibitors for the treatment of genetic alterations in peripheral neuropathies.
Article
Multidisciplinary Sciences
Jyoti Sharma, Ming Du, Eric Wong, Venkateshwar Mutyam, Yao Li, Jianguo Chen, Jamie Wangen, Kari Thrasher, Lianwu Fu, Ning Peng, Liping Tang, Kaimao Liu, Bini Mathew, Robert J. Bostwick, Corinne E. Augelli-Szafran, Hermann Bihler, Feng Liang, Jerome Mahiou, Josef Saltz, Andras Rab, Jeong Hong, Eric J. Sorscher, Eric M. Mendenhall, Candice J. Coppola, Kim M. Keeling, Rachel Green, Martin Mense, Mark J. Suto, Steven M. Rowe, David M. Bedwell
Summary: In this study, compounds with readthrough activity were identified and shown to reduce premature termination associated with cystic fibrosis by lowering eRF1 levels. These compounds, including SRI-41315 and SRI-37240, have potential as promising treatment strategies for diseases caused by PTCs.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Roland N. Wagner, Michael Wiessner, Andreas Friedrich, Johanna Zandanell, Hannelore Breitenbach-Koller, Johann W. Bauer
Summary: This review summarizes the current understanding of translation termination and highlights newly discovered pathways that influence its fidelity. It also describes the mechanisms involved in the recognition and readthrough of premature termination codons (PTCs) and reports on compounds that induce PTC readthrough. The ongoing attempts of personalized nonsense suppression therapy in different disease contexts are also reviewed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Martine Palma, Fabrice Lejeune
Summary: Recognition of the stop codon is crucial for terminating translation and synthesizing the correct size protein. Stop codon readthrough can occur under specific conditions, leading to different protein isoforms, and has potential therapeutic implications for genetic diseases caused by nonsense mutations.
BIOLOGICAL REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Maciej Dabrowski, Zuzanna Bukowy-Bieryllo, Claire L. Jackson, Ewa Zietkiewicz
Summary: The study identified several non-aminoglycoside compounds with potential to induce PTC-readthrough, which demonstrated minimal negative impact on cell viability and function compared to aminoglycosides, although with lower efficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Michael Popadynec, Alireza Baradaran-Heravi, Benjamin Alford, Scott A. Cameron, Keith Clinch, Jennifer M. Mason, Phillip M. Rendle, Olga Zubkova, Zhonghong Gan, Hui Liu, Oscar Rebollo, Dennis M. Whitfield, Fengyang Yan, Michel Roberge, David A. Powell
Summary: Aminoglycosides have the potential to induce readthrough of premature termination codons, which could be valuable in treating genetic diseases. Modification of aminoglycoside compounds to reduce cellular toxicity while maintaining readthrough activity is a strategy documented in this study.
ACS MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Leire Torices, Janire Mingo, Isabel Rodriguez-Escudero, Teresa Fernandez-Acero, Sandra Luna, Caroline E. Nunes-Xavier, Jose Lopez, Fatima Mercadillo, Maria Curras, Miguel Urioste, Maria Molina, Victor J. Cid, Rafael Pulido
Summary: Heterozygous germline mutations in PTEN gene are associated with hamartomas, tumors, and neurodevelopmental disorders. PTEN acts as a phosphatase, counteracting the pro-oncogenic function of PI3K. This study characterized several PTEN variants found in PHTS patients and observed complex patterns of loss of function, altered localization, and altered cleavage by caspase-3.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Pathology
Caroline E. Nunes-Xavier, Maite Emaldi, Ingrid J. Guldvik, Hakon Ramberg, Kristin A. Tasken, Gunhild M. Maelandsmo, Oystein Fodstad, Roberto Llarena, Rafael Pulido, Jose I. Lopez
Summary: MVP is expressed in prostate cancer and positively correlated with biochemical recurrence, and it is also positively correlated with androgen receptor and immune checkpoint protein B7-H3, but not with PD-L1 or PTEN expression. MVP may play a significant role in immune regulation and drug resistance in prostate cancer.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Biology
Annick Laruelle, Andre Rocha, Claudia Manini, Jose I. Lopez, Elena Inarra
Summary: In this study, interactions between cancer cells were explored using the hawk-dove game. The heterogeneity of tumors was analyzed by considering populations with 2 or 3 types of cells. The results showed that the best-off cell in the 2-type population game had a higher payoff than the best-off cell in the 3-type population game. The study highlights the importance of identifying intratumor heterogeneity in routine practice and suggests that therapeutic strategies preserving heterogeneity may slow down cancer growth.
BULLETIN OF MATHEMATICAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Caroline E. Nunes-Xavier, Maite Emaldi, Janire Mingo, Tove Oyjord, Gunhild M. Maelandsmo, Oystein Fodstad, Peio Errarte, Gorka Larrinaga, Roberto Llarena, Jose I. Lopez, Rafael Pulido
Summary: This study investigated the expression and activity of PDK1-4 in renal cancer cells and found that PDK2 and PDK3 protein expression were associated with lower overall patient survival, while PDK1 protein expression correlated with higher patient survival. The study also revealed a molecular association between PDK2 and PDK3 expression and the PI3K signaling pathway, as well as T cell infiltration and exhausted CD8 T cells. Inhibition of PDK in human renal cancer cell lines resulted in lower cell viability accompanied by an increase in pAKT.
SCIENTIFIC REPORTS
(2023)
Article
Cardiac & Cardiovascular Systems
Fergie J. Losiniecki, Jose Lopez, Majd Jazaerly, Kristina Menchaca, Vivek Kothari, Brendon Cornett, Christopher N. Ochner, Robert Chait
Summary: This study aims to evaluate the predictive value of serum troponin I level on in-hospital mortality in COVID-19 patients during the first 24 hours of admission. The study found that a negative troponin I level during the first 24 hours of admission was associated with lower rates of in-hospital mortality and cardiac complications.
IJC HEART & VASCULATURE
(2023)
Article
Engineering, Biomedical
Massimo Salvi, Claudia Manini, Jose I. Lopez, Dario Fenoglio, Filippo Molinari
Summary: This study introduces a novel approach that combines diagnosis-specific immunohistochemical (IHC) staining and deep learning techniques to provide reliable stratification of prostate glands. The system achieved noteworthy results in specific-class gland quantification on whole slides, with a mean Dice Score Coefficient of 90.36% and a mean absolute error of 1.64%. The findings demonstrate the potential of this system as a valuable support tool for pathologists, reducing workload and decreasing diagnostic inter-observer variability.
COMPUTERIZED MEDICAL IMAGING AND GRAPHICS
(2023)
Article
Multidisciplinary Sciences
Leire Torices, Caroline Nunes-Xavier, Jose I. Lopez, Rafael Pulido
Summary: PTEN is a tumor suppressor gene frequently mutated in human tumors, and its mutations are the diagnostic marker for PTEN Hamartoma Tumor Syndrome (PHTS), a disorder characterized by multiple hamartomas, cancer predisposition, and neurodevelopmental alterations. This study generated and characterized anti-PTEN monoclonal antibodies recognizing the PTEN C2-domain, which can be used to study the expression and function of PTEN isoforms and mutations associated with disease. These antibodies are suitable for investigating the pathogenicity of PTEN C-terminal truncations that have lost the PTEN C-terminal epitopes but retain stability and function.
Article
Immunology
Laura Amo, Hemanta K. Kole, Bethany Scott, Chen-Feng Qi, Ludmila Krymskaya, Hongsheng Wang, Louis H. Miller, Chris J. Janse, Silvia Bolland
Summary: Infection with Plasmodium can cause kidney injury as a side effect of the host immune response. However, animal models suggest that the parasite can also protect against autoimmune nephritis. The factors responsible for kidney damage in humans and the mechanisms of protection in animal models are not well understood.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Inigo Terren, Victor Sanda, Ainhoa Amarilla-Irusta, Ainara Lopez-Pardo, Arrate Sevilla, Gabirel Astarloa-Pando, Laura Amo, Olatz Zenarruzabeitia, Luca Scorrano, Francisco Borrego
Summary: Cytokine-induced memory-like (CIML) NK cells, stimulated with IL-12, IL-15 and IL-18, show promise in cancer immunotherapy. However, the mitochondrial dynamics of these cells remain unknown. This study found that IL-12/15/18 stimulation led to decreased viability and increased superoxide levels in NK cells, along with changes in mitochondrial morphology possibly mediated by decreased levels of OPA1 protein. While mitophagy was slightly impaired, an increase in autophagic flux was observed. These findings are important for improving the mitochondrial fitness and therapeutic efficacy of IL-12/15/18-stimulated NK cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Geriatrics & Gerontology
Teresa Flores Ruano, Emiel O. Hoogendijk, Luis Romero Rizos, Gabriel Ariza Zafra, Matilde Leon Ortiz, Carmen Luengo Marquez, Elena Martin Senbastia, Jose Luis Navarro Lopez, Miguel Fernandez Sanchez, Rafael Garcia Molina, Almudena Avendano Cespedes, Victoria Sanchez-Flor Alfaro, Cristina Gomez Ballesteros, Rita Lopez Bru, Elsa Dent, Pedro Abizanda
Summary: This study examined the association between resting metabolic rate (RMR) and incident disability and mobility decline in older adults over a 10-year period. The results showed that higher RMR was associated with a lower risk of disability and mobility decline, especially in frail individuals.
AGING CLINICAL AND EXPERIMENTAL RESEARCH
(2023)
Review
Communication
Jose Luis Portela Lopez, Carlos Rodriguez Monroy
Summary: The advancements in technology have led to innovations in the organizational field, specifically in terms of product and service innovation. These innovations focus on understanding the real needs of consumers and the impact of their mental processes on their behavior. The integration of neuroeconomics and neuroscience provides a novel approach to analyze consumer behavior and the decision-making process.
REVISTA LATINA DE COMUNICACION SOCIAL
(2023)
Article
Medicine, General & Internal
Claudia Manini, Claudia Provenza, Leire Andres, Igone Imaz, Rosa Guarch, Raffaelle Nunziata, Jose I. Lopez
Summary: Tumor-to-tumor metastasis is a rare event that requires accurate identification by pathologists. It can be straightforward to identify when the tumors have different histologies, but can be challenging when two carcinomas with similar cytoarchitecture are present, one inside the other under the microscope. In this study, we report four cases involving clear cell renal cell carcinoma and highlight the difficulties in recognizing some of them. An appropriate clinical-pathological correlation, including a review of the patient's history and radiological exams, would greatly aid in routine identification of tumor-to-tumor metastases.
CLINICS AND PRACTICE
(2023)
Article
Medicine, General & Internal
Claudia Manini, Estibaliz Lopez-Fernandez, Nicola Cruciano, Alessandro Comandone, Jose I. Lopez
Summary: The correct diagnosis of mesothelial proliferations is a classic challenge for pathologists, and it holds significant clinical implications. These proliferations are frequently associated with recurrent hydrocele, and their morphological characteristics can resemble malignancy, leading to potential misdiagnosis.
CLINICS AND PRACTICE
(2023)