Tectorigenin attenuates the OGD/R-induced HT-22 cell damage through regulation of the PI3K/AKT and the PPARγ/NF-κB pathways

Tectorigenin (TEC) is an effective compound that derived from many plants, such as Iris unguicularis, Belamcanda chinensis and Pueraria thunbergiana Benth. Evidence suggested that TEC has anti-tumor, anti-oxidant activity, anti-bacterial and anti-inflammatory effects. In addition, there has some evidence indicated that TEC is a potential anti-stroke compound; however, its specific roles and associated mechanism have not yet been elucidated. In the present study, we aimed to investigate the anti-inflammatory, anti-oxidant activity and anti-apoptosis effects of TEC on oxygen-glucose deprivation/reperfusion (OGD/R)-induced HT-22 cells, and clarified the relevant mechanisms. Here, we observed that TEC significantly promoted cell survival, impeded cell apoptosis, inhibited ROS and inflammatory cytokines IL-1 beta, IL-6, TNF-alpha production in OGD/R-induced HT-22 cells. Moreover, TEC activated PI3K/AKT signal pathway, increased PPAR gamma expression and inhibited NF-kappa B pathway activation in OGD/R-induced HT-22 cells. Further studies indicated that PPAR gamma inhibitor GW9662 activated NF-kappa B pathway after TEC treatment in OGD/R-induced HT-22 cells. Also, PI3K/AKT inhibitor LY294002, PPAR gamma inhibitor GW9662 and NF-kappa B activator LPS both reversed the effects of TEC on OGD/R-induced HT-22 cell biology. Taken together, this research confirmed that TEC benefit to HT-22 cell survival and against OGD/R damage through the PI3K/AKT and PPAR gamma/NF-kappa B pathways. These results indicated that TEC might be an effective compound in the treatment for ischemic brain injury.

Automated summary

Tectorigenin (TEC) exhibits anti-inflammatory, anti-oxidant, and anti-apoptosis effects on oxygen-glucose deprivation/reperfusion (OGD/R)-induced HT-22 cells by activating the PI3K/AKT signal pathway, increasing PPARγ expression, and inhibiting NF-κB pathway activation. Furthermore, TEC may be a promising compound for the treatment of ischemic brain injury.