4.7 Article

Transcriptome and metabolome analyses reveal the pivotal role of hydrogen sulfide in promoting submergence tolerance in Arabidopsis

Journal

ENVIRONMENTAL AND EXPERIMENTAL BOTANY
Volume 183, Issue -, Pages -

Publisher

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envexpbot.2020.104365

Keywords

Hydrogen sulfide; Flooding stress; Cysteine; Transcriptome and metabolome; Submergence tolerance

Funding

  1. National Natural Science Foundation of China (NSFC) [31670254, 31770199, 31700215]
  2. Foundation of the Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education of China [lzujbky-2019-kb05]

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The study found that pretreating with exogenous H2S or Cys significantly improved Arabidopsis tolerance to submergence, suggesting a role of H2S-Cys balance in flood resistance. Transcriptomic and metabolomic analyses showed that exogenous H2S pretreatment activated hypoxia-responsive factors and influenced genes related to hypoxia sensing, resulting in limited cellular activities associated with growth.
As the global climate changes, the frequency and severity of flood disasters have increased significantly, seriously affecting agricultural production. Hydrogen sulfide (H2S), as a gaseous signal molecule, plays an important role in plant development and stress adaptation. Previous studies suggested that H2S acts as an oxygen sensor when animals experience hypoxia. However, the role of H2S in the response and adaptation to flooding-induced hypoxia stress in plants is poorly understood. Here, we report that pretreatment with exogenous H2S or Cys significantly improved tolerance to submergence in Arabidopsis. Total submergence of soil-grown Arabidopsis increased the levels of endogenous H2S and its main endogenously generated product, cysteine (Cys) over time, and altered the expression of H2S or Cys biosynthesis- or metabolism-related genes, implying that the H2S-Cys homeostasis may contribute to regulation of tolerance to flooding. Transcriptomic and metabolomic analyses demonstrated that exogenous H2S-pretreatment activated hypoxia-responsive transcription factors and hypoxia sensing-related genes in a timely manner and limited cellular activities associated with growth through regulation of hormonal signaling, but had less influence on transcripts associated with photosynthesis. H2S-pretreatment also altered the levels of metabolites, particularly of amino acids and plant hormones, during submergence. Taken together, this study provides new insight into how H2S regulates tolerance to submergence-induced hypoxia stress and lays the foundation to design new strategies for the improvement of submergence resistance in crops.

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Article Astronomy & Astrophysics

Search for the chiral magnetic effect in Au plus Au collisions at ?sNN=27 GeV with the STAR forward event plane detectors

B. E. Aboona, J. Adam, L. Adamczyk, J. R. Adams, I. Aggarwal, M. M. Aggarwal, Z. Ahammed, D. M. Anderson, E. C. Aschenauer, J. Atchisona, V. Bairathi, W. Baker, J. G. Ball Cap, K. Barish, R. Bellwied, P. Bhagat, A. Bhasin, S. Bhatta, J. Bielciko, J. Bielcikova, J. D. Brandenburg, X. Z. Cai, H. Caines, M. Calderon de la Barca Sanchezi, D. Cebrai, J. Ceskao, I. Chakaberia, P. Chaloupka, B. K. Chan, Z. Chang, D. Chen, J. Chen, J. H. Chen, Z. Chen, J. Cheng, Y. Cheng, S. Choudhury, W. Christie, X. Chu, H. J. Crawford, M. Csanad, G. Dale-Gau, A. Das, M. Daugherity, I. M. Deppnert, A. Dhamija, L. Di Carlobi, L. Didenko, P. Dixitw, X. Dong, J. L. Drachenberg, E. Duckworth, J. C. Dunlop, J. Engelage, G. Eppley, S. Esumi, O. Evdokimovm, A. Ewigleben, O. Eyser, R. Fatemi, S. Fazio, C. J. Feng, Y. Feng, E. Finch, Y. Fisyak, F. A. Flor, C. Fu, C. A. Gagliardi, T. Galatyuk, F. Geurts, N. Ghimire, A. Gibson, K. Gopal, X. Gou, D. Grosnick, A. Gupta, W. Guryn, A. Hamedd, Y. Han, S. Harabasz, M. D. Harastyi, J. W. Harris, H. Harrison, W. He, X. H. He, Y. He, N. Herrmann, L. Holub, C. Hu, Q. Hu, Y. Hu, H. Huang, H. Z. Huangj, S. L. Huang, T. Huang, X. Huang, Y. Huang, Y. Huang, T. J. Humanic, D. Isenhower, M. Isshiki, W. W. Jacobs, A. Jalotra, C. Jena, A. Jentsch, Y. Ji, J. Jia, C. Jin, X. Ju, E. G. Judd, S. Kabana, M. L. Kabir, S. Kagamaster, D. Kalinkin, K. Kang, D. Kapukchyan, K. Kauder, H. W. Ke, D. Keane, M. Kelsey, Y. V. Khyzhniak, D. P. Kikola, B. Kimelman, D. Kincses, I. Kisel, A. Kiselev, A. G. Knospe, H. S. Ko, K. Kosarzewski, L. Kramarik, L. Kumar, S. Kumar, R. Kunnawalkam Elayavalli, R. Lacey, J. M. Landgraf, J. Lauret, A. Lebedev, J. H. Lee, Y. H. Leung, N. Lewis, C. Li, C. Li, W. Li, X. Li, Y. Li, Y. Li, Z. Li, X. Liang, Y. Liang, R. Licenik, T. Lin, M. A. Lisa, C. Liu, F. Liu, H. Liu, H. Liu, L. Liu, T. Liu, X. Liu, Y. Liu, Z. Liu, T. Ljubicic, W. J. Llope, O. Lomicky, S. Longacre, E. Loyd, T. Lu, S. Lukow, F. Luo, L. Ma, R. Ma, Y. G. Ma, N. Magdy, D. Mallick, S. Margetis, C. Markert, H. S. Matis, J. A. Mazer, G. McNamara, K. Mi, S. Mioduszewski, B. Mohanty, I. Mooney, A. Mukherjee, M. I. Nagy, A. S. Nain, J. D. Nam, Md. Nasim, D. Neff, J. M. Nelson, D. B. Nemes, M. Nie, T. Niida, R. Nishitani, T. Nonaka, A. S. Nunes, G. Odyniec, A. Ogawa, S. Oh, K. Okubo, B. S. Page, R. Pak, J. Pan, A. Pandav, A. K. Pandey, T. Pani, A. Paul, B. Pawlik, D. Pawlowska, C. Perkins, J. Pluta, B. R. Pokhrel, M. Posik, T. Protzman, V. Prozorova, N. K. Pruthi, M. Przybycien, J. Putschke, Z. Qin, H. Qiu, A. Quintero, C. Racz, S. K. Radhakrishnan, N. Raha, R. L. Ray, R. Reed, H. G. Ritter, C. W. Robertson, M. Robotkova, M. A. Rosales Aguilar, D. Roy, P. Roy Chowdhury, L. Ruan, A. K. Sahoo, N. R. Sahoo, H. Sako, S. Salur, S. Sato, W. B. Schmidke, N. Schmitz, F. -J. Seck, J. Seger, R. Seto, P. Seyboth, N. Shah, P. V. Shanmuganathan, M. Shao, T. Shao, M. Sharma, N. Sharma, R. Sharma, S. R. Sharma, A. I. Sheikh, D. Y. Shen, K. Shen, S. S. Shi, Y. Shi, Q. Y. Shou, F. Si, J. Singh, S. Singha, P. Sinha, M. J. Skoby, N. Smirnov, Y. Sohngen, Y. Song, B. Srivastava, T. D. S. Stanislaus, M. Stefaniak, D. J. Stewart, B. Stringfellow, Y. Su, A. A. P. Suaide, M. Sumbera, C. Sun, X. Sun, Y. Sun, Y. Sun, B. Surrow, Z. W. Sweger, P. Szymanski, A. Tamis, A. H. Tang, Z. Tang, T. Tarnowsky, J. H. Thomas, A. R. Timmins, D. Tlusty, T. Todoroki, C. A. Tomkiel, S. Trentalange, R. E. Tribble, P. Tribedy, T. Truhlar, B. A. Trzeciak, O. D. Tsai, C. Y. Tsang, Z. Tu, T. Ullrich, D. G. Underwood, I. Upsal, G. Van Buren, J. Vanek, I. Vassiliev, V. Verkest, F. Videbaek, S. A. Voloshin, F. Wang, G. Wang, J. S. Wang, X. Wang, Y. Wang, Y. Wang, Y. Wang, Z. Wang, J. C. Webb, P. C. Weidenkaff, G. D. Westfall, D. Wielanek, H. Wieman, G. Wilks, S. W. Wissink, R. Witt, J. Wu, J. Wu, X. Wu, Y. Wu, B. Xi, Z. G. Xiao, W. Xie, H. Xu, N. Xu, Q. H. Xu, Y. Xu, Y. Xu, Z. Xu, Z. Xu, G. Yan, Z. Yan, C. Yang, Q. Yang, S. Yang, Y. Yang, Z. Ye, Z. Ye, L. Yi, K. Yip, Y. Yu, H. Zbroszczyk, W. Zha, C. Zhang, D. Zhang, J. Zhang, S. Zhang, X. Zhang, Y. Zhang, Y. Zhang, Y. Zhang, Z. J. Zhang, Z. Zhang, Z. Zhang, F. Zhao, J. Zhao, M. Zhao, C. Zhou, J. Zhou, S. Zhou, Y. Zhou, X. Zhu, M. Zurek, M. Zyzak

Summary: This study presents a low-energy search for the Chiral Magnetic Effect (CME) in Au+Au collisions at RHIC and finds consistency between CME and flow-driven background hypothesis based on the definition of event planes and centrality range. This work opens up a possible roadmap for future CME exploration with high-statistics data from RHIC.

PHYSICS LETTERS B (2023)

Article Oncology

Association of modifiable lifestyle with colorectal cancer incidence and mortality according to metabolic status: prospective cohort study

Peng Xie, Siqing Wu, Zichong Kuo, Huidong Tian, Qiangsheng He, Yanfei Li, Ningning Mi, Linmin Hu, Haitong Zhao, Wenjing Li, Bin Xia, Jinqiu Yuan, Kehu Yang, Changhua Zhang, Yulong He

Summary: This study aimed to investigate the individual and joint effects of modifiable healthy lifestyle and metabolic health status on colorectal cancer incidence and mortality. The results showed that adherence to a healthy lifestyle could substantially reduce the burden of colorectal cancer, and behavioral lifestyle changes should be encouraged for colorectal cancer prevention even in participants with metabolic syndrome.

FRONTIERS IN ONCOLOGY (2023)

Article Medicine, General & Internal

How about the evidence assessment tools used in education and management systematic reviews?

Hui Lan, Xuan Yu, Zhe Wang, Ping Wang, Yajia Sun, Zijun Wang, Renfeng Su, Ling Wang, Junxian Zhao, Yue Hu, Shouyuan Wu, Mengjuan Ren, Kehu Yang, Xingrong Liu, Yaolong Chen

Summary: This study aims to examine the usage of evidence assessment tools in systematic reviews of management and education. The results show that only 34.8% of the systematic reviews used evidence assessment tools, with a total of 66 different tools being utilized. "Risk of Bias" and its updated version were the most frequently used tools. The study also highlights the need for improved understanding and reporting of evidence assessment tools among researchers and users.

FRONTIERS IN MEDICINE (2023)

Article Plant Sciences

Wheat MEDIATOR25, TaMED25, plays roles in freezing tolerance possibly through the jasmonate pathway

Jingqiu Xia, Jiawen Liang, Mengmeng Yu, Rui Wang, Chen Sun, Huishan Song, Qinghua Xu, Jing Cang, Yuying Wang, Da Zhang

Summary: The MED complex acts as a bridge to regulate transcription by connecting transcription factors and specific gene promoters. This study demonstrates the important role of MED25 in freezing tolerance in wheat, possibly through its interaction with the jasmonate signaling pathway and cold-responsive genes.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

Resource availability and herbivory alter defence-growth-reproduction trade-offs in a masting Mediterranean pine

Asier R. Larrinaga, Luis Sampedro, Rafael Zas

Summary: This study investigated the allocation to growth, reproduction, and defence in maritime pine under different resource availability and simulated herbivory treatments. The results showed that fertilization increased tree growth and seed quantity but not seed quality. Needle clipping and methyl-jasmonate treatment increased needle resin and phenol concentrations. Overall, there were complex interactions among the life-history dimensions, suggesting that pairwise approaches are insufficient to unravel these complexities.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

The Chrysanthemum morifolium β-carotene hydroxylase gene CmBCH1 promotes tolerance to high light by enhancement of the xanthophyll cycle

Shuang Han, Shuxian Li, Ya Li, Qingchen Zhang, Yuanyuan Xu, Wenjing Wang, Xiao Qin Zhu, Dongli Pei

Summary: The xanthophyll cycle plays a significant role in protecting plants from membrane peroxidation induced by intense light exposure. This study identified 240 differentially expressed genes associated with respiratory chain, lipid metabolism, antioxidant activity, and flavonoid metabolism. The overexpression of the CmBCH1 gene resulted in the upregulation of genes involved in scavenging reactive oxygen species and encoding antioxidants, leading to the accumulation of xanthophyll, zeaxanthin, chlorophylls, and anthocyanins. The study highlighted the potential of BCH1 in regulating the xanthophyll cycle and enhancing tolerance to intense light stress in transgenic plants.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

Enhanced phytoremediation of PCBs-contaminated soil by co-expressing tfdB and bphC in Arabidopsis aiding in metabolism and improving toxicity tolerance

Yuzhu Ding, Hejun Ren, Xinyu Hao, Ruonan Zhang, Jianjun Hao, Jinliang Liu, Hongyu Pan, Yan Wang

Summary: This study demonstrated that co-expressing two exogenous genes, tfdB and bphC, in Arabidopsis thaliana improved the tolerance and removal efficiency of PCB28. Transgenic plants also exhibited increased enzymatic activities under PCB28 stress. The co-expression of tfdB and bphC in A. thaliana resulted in nearly twofold increase in PCB28 removal rates from soil. This research suggests that co-expressing two genes holds great potential for enhancing phytoremediation efficiency and mitigating PCB-induced toxicity stress on plants.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

The intricate world of trichome development: From signaling pathways to transcriptional regulation

Nosheen Kabir, Sumbal Wahid, Shoaib Ur Rehman, Ghulam Qanmber

Summary: Trichomes are specialized plant structures that protect plants and are regulated by complex gene networks and signaling pathways. Gene expression, cell cycle regulation, and differentiation determine whether cells become trichomes. Transcription factors, epigenetic modifications, and phytohormones play important roles in trichome development. The integration of phytohormonal and transcriptional networks contributes to the diversity and adaptability of trichomes in plants.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

Soil microorganisms buffer the reduction in plant growth and physiological performance under combined abiotic stress in the halophyte Salicornia ramosissima

Enrique Mateos-Naranjo, Jesus Alberto Perez-Romero, Giacomo Puglielli, Javier Lopez-Jurado, Jennifer Mesa-Marin, Eloisa Pajuelo, Ignacio David Rodriguez-Llorente, Susana Redondo-Gomez

Summary: The impact of multifactorial abiotic stress combinations on plant functional responses remains controversial. The halophyte Salicornia ramosissima showed unique functional responses depending on the specific stress factors involved. Under extreme conditions, there was a more negative impact on plant functional traits, but the presence of beneficial microorganisms, especially in combination with elevated atmospheric CO2 concentration, mitigated these negative effects.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

Alternative oxidase 2 influences Arabidopsis seed germination under salt stress by modulating ABA signalling and ROS homeostasis

Mengjiao Ruan, Wenliang He, Rui He, Xiangxiang Wang, Jinxin Wei, Yujie Zhu, Ruiling Li, Zhijun Jiang, Xiaofan Na, Xiaomin Wang, Yurong Bi

Summary: The study reveals the important role of AOX2 in Arabidopsis seed germination by regulating ABA signal and ROS homeostasis under salt stress. Additionally, ABI3/ABI4 are essential for salt-induced AOX2 expression.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

Characterization of epigenetic modifications in a plant-specific glutaredoxin-mediated repression of stress-responsive gene expression

Ning Li, Peiyao Yu, Yanling Zeng, Jiali Chen, Wenhai Yang, Guannan Qin, Shenxiu Du, Xiao Han, Li-Jun Huang

Summary: In eukaryotic cells, epigenetic modifications of DNA and histones play a crucial role in gene expression regulation. ROXY19, a plant-specific CC-type glutaredoxin, was found to strongly repress a subset of genes regulated by class II TGA factors through its association with the TPL/TPR family. The ectopic expression of ROXY19 in Arabidopsis plants led to hypersensitivity to xenobiotic chemicals due to the silencing of detoxification pathway genes.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

The NIN-LIKE PROTINE1 (CsNLP1) transcription factor is involved in modulating the nitrate response in cucumber seedlings

Yang Li, Chenyang Feng, Yijing Xing, Meng Li, Xiaoning Wang, Qingjie Du, Huaijuan Xiao, Juanqi Li, Jiqing Wang

Summary: In this study, researchers found that CsNLP1 gene in cucumber plays an important role in nitrogen utilization. The study also revealed the regulation mechanism of CsNLP1 in cucumber growth and nitrogen assimilation.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

Genome-wide identification of C2H2-ZFPs and functional analysis of BcZAT12 and BcZAT10 under cold stress in non-heading Chinese cabbage

Lei Chen, Cheng Jiang, Li Ye, Yue Gao, Xilin Hou

Summary: This study identified and characterized 163 C2H2-type zinc finger proteins in non-heading Chinese cabbage. Two of these proteins, BcZAT12 and BcZAT10, were found to be closely related to cold tolerance and acted as transcriptional repressors in regulating cold resistance. Furthermore, BcZAT12 was shown to directly bind to the promoter of BcCBF1 and inhibit its activity. Additionally, interactions between BcZAT12 and other proteins such as BcABF2/4 were observed. These findings provide insights into the functional roles and regulatory mechanisms of C2H2-ZFPs in non-heading Chinese cabbage under cold stress.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

Effect of Nano Biochar addition and deficit irrigation on growth, physiology and water productivity of quinoa plants under salinity conditions

Ommolbanin Tourajzadeh, Halimeh Piri, Amir Naserin, Mohammad mahdi Cahri

Summary: The combination of NB and reduced irrigation water depth can mitigate the negative effects of salinity and drought stresses on quinoa and improve its water productivity. The use of NB up to 2% alleviated salinity and drought stress and increased yield, while decreasing salinity and irrigation water depth increased water productivity. The appropriate use of NB can reduce the negative effects of stress and promote the sustainable production of quinoa.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

Nonuniform salinity regulate leaf characteristics and improve photosynthesis of cherry tomatoes under high salinity

Jing Li, Yujie Wu, Xiaohui Feng, Tabassum Hussain, Kai Guo, Xiaojing Liu

Summary: This study investigated the growth and physiological responses of cherry tomatoes under different salt conditions and found that nonuniform salinity conditions improved photosynthetic characteristics, water use efficiency, and fruit yield. These findings are important for improving tomato productivity on salt-affected lands.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

Auxin mediated elevated CO2-induced stolon growth and soluble sugar accumulation in creeping bentgrass

Ruonan Li, Ruying Wang, Meng Li, Yunpu Zheng, Xiaxiang Zhang, Zhimin Yang, Jingjin Yu

Summary: The study found that elevated CO2 can increase stolon growth and carbohydrate accumulation in stolon nodes and internodes. Foliar application of auxin enhances this effect, while auxin transport inhibitor has no effect.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

The universal and divergent roles of ethylene in rice and some other crop plants under salt stress

Jian-Jun Tao, Cui-Cui Yin, Yang Zhou, Yi-Hua Huang, Shou-Yi Chen, Jin-Song Zhang

Summary: Soil salinization is a major obstacle for agriculture sustainability, as it severely affects plant growth and crop yield. Ethylene, a stress hormone, plays a crucial role as a signal molecule in coordinating plant growth and stress response in the model plant Arabidopsis. However, the roles and mechanisms of ethylene in most crop plants under salinity remain undefined. This review summarizes the universal roles of ethylene and focuses on its divergent roles in rice and other crop species under salinity, particularly in terms of ethylene biosynthesis and signal transduction. The contradictions between ethylene production and signaling in salt response are also discussed.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)

Article Plant Sciences

Plant defense mechanisms against ozone stress: Insights from secondary metabolism

Chang Guo, Xiaona Wang, Qi Wang, Zipeng Zhao, Bing Xie, Lang Xu, Ruijie Zhang

Summary: This review provides an overview of the effects of ozone pollution on plant secondary metabolism. It explores the role of plant secondary metabolism in defense against ozone stress and adaptation to ozone-polluted environments, while summarizing the severity of ozone pollution.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2024)