Review
Nutrition & Dietetics
Rakesh Balachandar, Raghu Pullakhandam, Bharati Kulkarni, Harshpal Singh Sachdev
Summary: This study found that cholecalciferol was more effective than ergocalciferol in improving vitamin D status and regulating PTH levels, regardless of participant demographics, dosage, and vehicle of supplementation.
Article
Chemistry, Multidisciplinary
Yonghao Mi, Enliang Wang, Zack Dube, Tian Wang, A. Y. Naumov, D. M. Villeneuve, P. B. Corkum, Andre Staudte
Summary: Using near-infrared, femtosecond laser pulses and coincidence momentum imaging, it is found that the dominant channel after photoionization of a deuterium molecular dimer (D-2-D-2) is the ejection of a deuterium atom within a few hundred femtoseconds, leading to the formation of D-3(+). This pathway of D-3(+) formation from ultracold D-2-D-2 gas may provide insights into the high abundance of H-3(+) in the interstellar medium.
Article
Nutrition & Dietetics
Andrea Hanel, Cor Veldhuizen, Carsten Carlberg
Summary: This study compared the modulation of the transcriptome of human peripheral blood mononuclear cells (PBMCs) by the vitamin D metabolites 25-hydroxyvitamin D-3 (25(OH)D-3), 25(OH)D-2, and 1 alpha,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3). The findings showed that 25(OH)D-3 and 25(OH)D-2 were equally potent in modulating the transcriptome of PBMCs at supra-physiological concentrations, possibly by directly activating the vitamin D receptor.
FRONTIERS IN NUTRITION
(2022)
Article
Immunology
Louise R. Durrant, Giselda Bucca, Andrew Hesketh, Carla Moller-Levet, Laura Tripkovic, Huihai Wu, Kathryn H. Hart, John C. Mathers, Ruan M. Elliott, Susan A. Lanham-New, Colin P. Smith
Summary: Vitamin D plays a crucial role in maintaining bone health and calcium balance, and also has broad effects on cellular physiology and the immune system. This study examines the differences in gene expression following supplementation with vitamin D-2 and D-3, and suggests the existence of ethnic variations in the response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lisa Marie Schmitz, Alina Kinner, Kirsten Althoff, Katrin Rosenthal, Stephan Luetz
Summary: The study identified a novel strain, Kutzneria albida, capable of producing 25-OH-D-2 and 25-OH-D-3, with significantly increased product concentrations after the addition of 2-hydroxypropyl beta-cyclodextrin. This suggests that K. albida is a promising candidate for the biotechnological production of VD2 and VD3 metabolites.
Article
Pharmacology & Pharmacy
Yingyu Lu, Jianqiang Chen, Xueling He, Shuoxi Xu, Yong-er Chen, Jie Gao, Shaozhen Hou
Summary: The single use of geniposide and vitamin D-3 showed significant improvement in symptoms of UC, while the combination treatment had less beneficial effects on UC in mice. Geniposide could inhibit the activation of p38 MAPK and restrict the vitamin D receptor signaling stimulated by vitamin D-3.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medical Laboratory Technology
Carl Jenkinson, Reena Desai, Andrzej T. Slominski, Robert C. Tuckey, Martin Hewison, David J. Handelsman
Summary: The study developed an LC-MS/MS method to measure 13 vitamin D metabolites simultaneously, providing new insights into the circulating vitamin D metabolite pathways.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Simon Hsu, Leila R. Zelnick, Yvonne S. Lin, Cora M. Best, Bryan R. Kestenbaum, Kenneth E. Thummel, Andrew N. Hoofnagle, Ian H. de Boer
Summary: The study confirmed the correlation between 24,25-dihydroxyvitamin D (24,25(OH)(2)D) and the clearance rate of 25-hydroxyvitamin D (25(OH)D-3) as a marker for clearance. Participants with chronic kidney disease, kidney failure, Black participants, and those with lower serum albumin had lower VDMR.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Neeraj Singh, Judith Taibon, Stephan Pongratz, Christian Geletneky
Summary: Vitamin D is an important parameter for the determination of calcium and phosphate regulation in the human body. LC-MS/MS methods are considered the most accurate for quantification. This study utilized quantitative NMR spectroscopy to determine the absolute content of vitamin D molecules and investigate their stability in borderline polar solvents. Isomerization of the analytes was observed, which can affect the quantification using LC and GC analytical techniques.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Lianrong Zhou, Hongcheng Ni, Zhejun Jiang, Junjie Qiang, Wenyu Jiang, Wenbin Zhang, Peifen Lu, Jin Wen, Kang Lin, Meifang Zhu, Reinhard Doerner, Jian Wu
Summary: The formation of trihydrogen cation driven by light has been of great interest due to its important role in initiating chemical reactions in interstellar clouds. Previous studies focused on the formation of H-3(+) or D-3(+) from unimolecular reactions, but we observed and characterized the ultrafast formation of D-3(+) from a bimolecular reaction. Molecular dynamics simulations provided an intuitive representation of the reaction dynamics, which matched well with experimental observations. Additionally, we demonstrated control over the emission direction of D-3(+) using a tailored two-colour femtosecond laser field, based on the mechanism known for light control of electron localization in single molecules.
Article
Nutrition & Dietetics
RuTong Wang, Weijing Wang, Ping Hu, Ronghui Zhang, Xue Dong, Dongfeng Zhang
Summary: This study aimed to evaluate the association between dietary vitamin D intake and serum vitamin D concentrations with cognitive performance in older Americans. The results showed a positive association between dietary vitamin D intake, serum total 25-hydroxyvitamin D, and cognitive performance, with no significant gender differences in the associations. Further research is needed to clarify the effects of dietary vitamin D intake and specific serum D concentrations on cognitive performance.
Article
Nutrition & Dietetics
Anna Leszczyszyn, Sylwia Hnitecka, Marzena Dominiak
Summary: This study assessed the impact of VD3 deficiency on the development of malocclusions, finding a link between VD3 deficiency and maxillary underdevelopment, as well as a higher risk of narrowed upper arch, crowding, and crossbite. The findings suggest that VD3 deficiency may lead to dentoalveolar malocclusion, especially transverse constriction of the maxilla.
Article
Biochemistry & Molecular Biology
Robert C. Tuckey, Chloe Y. S. Cheng, Lei Li, Yuhan Jiang
Summary: Our study found that 25-hydroxyvitamin D3 (25(OH)D3) can exist in the human circulation in the sulfated form, and can be metabolized by vitamin D-metabolizing cytochromes P450 (CYPs). Specifically, CYP27A1 can convert vitamin D3 sulfate to 25(OH)D3 3-sulfate, while CYP11A1 can convert vitamin D3 sulfate to 20(OH)D3 3-sulfate, but at a lower rate.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Celine Legros, Anne Rojas, Clemence Dupre, Chantal Brasseur, Isabelle Riest-Fery, Olivier Muller, Jean-Claude Ortuno, Olivier Nosjean, Sophie-Penelope Guenin, Gilles Ferry, Jean A. Boutin
Summary: D3/D2 sub-specificity is a complex problem to solve due to the lack of easy structural biology of G-protein coupled receptors. This study used directed mutagenesis and basic binding experiments to explore a rational approach for the discovery of highly specific D3 ligands over D2 ligands. Stable expression of receptors in HEK cells was used to construct mutants and chimeras of D2 and D3 receptors, and their binding characteristics were analyzed using standard binding conditions. The study demonstrated the feasibility and potential of the approach.
Article
Chemistry, Multidisciplinary
Michal Chojnacki, Jakub Anisiewicz, Ilona Lesniowska, Marta Kinga Lemieszek
Summary: Vitamin D3 deficiency is a pandemic with serious health consequences, including chronic respiratory diseases. Vitamin D supplementation has proven ineffective in improving this situation. This study demonstrates the usefulness and safety of delivering 1,25(OH)2-vitamin D3 and its precursor directly into the respiratory tract through nebulization. This method restores physiological calcitriol levels in animals with vitamin D deficiencies.
APPLIED SCIENCES-BASEL
(2023)
Review
Oncology
Justine Vanhevel, Lieve Verlinden, Stefanie Doms, Hans Wildiers, Annemieke Verstuyf
Summary: The active form of vitamin D-3, 1,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3), plays a key role in regulating calcium and phosphate levels. Low levels of 25(OH)D, a marker for 1,25(OH)(2)D-3, are associated with an increased risk of breast cancer (BC) development. However, vitamin D-3 supplementation does not show a significant decrease in BC incidence. 1,25(OH)(2)D-3 or its analogues may have more potential anticancer activity when combined with existing cancer therapies.
ENDOCRINE-RELATED CANCER
(2022)
Article
Immunology
Pieter-Jan Martens, Javier Centelles-Lodeiro, Darcy Ellis, Dana Paulina Cook, Gabriele Sassi, Lieve Verlinden, Annemieke Verstuyf, Jeroen Raes, Chantal Mathieu, Conny Gysemans
Summary: The study found that consuming 800 IU/day of vitamin D can reduce the incidence of type 1 diabetes in female NOD mice, increase the number of Treg cells in lymphoid organs, and alter the characteristics of the intestinal microbiota.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Endocrinology & Metabolism
Justine Vanhevel, Lieve Verlinden, Shauni Loopmans, Stefanie Doms, Iris Janssens, Sien Bevers, Steve Stegen, Hans Wildiers, Annemieke Verstuyf
Summary: This study demonstrates that the addition of a potent vitamin D-3 analog to selective CDK4/6 inhibitor treatment enhances the antiproliferative effects in ER+ breast cancer.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Chemistry, Applied
Tania Gonzalez-Garcia, Annemieke Verstuyf, Lieve Verlinden, Susana Fernandez, Miguel Ferrero
Summary: Three novel 19-nor-1 alpha,25-dihydroxyvitamin D-3 derivatives modified at C1 and/or C3 with an amino group were synthesized to investigate the impact of substituting one or both hydroxyl groups of the A-ring with amino groups on the affinity for VDR and hDBP, as well as the antiproliferative activity. The A-ring precursors were obtained through a desymmetrization reaction of a prochiral 1,3-diol catalyzed by Pseudomonas cepacia lipase, and the diastereoisomers were identified by NMR spectroscopy.
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Uxia Gomez-Bouzo, Anna Y. Belorusova, Marcos L. Rivadulla, Hugo Santalla, Lieve Verlinden, Annemieke Verstuyf, Maria J. Ferronato, Alejandro C. Curino, Maria M. Facchinetti, Yagamare Fall, Generosa Gomez, Natacha Rochel
Summary: This article describes the structural analysis and biological evaluation of two derivatives of 1,25-dihydroxyvitamin D3 with modifications limited to the replacement of the 25-hydroxyl group by a 25-amino or 25-nitro groups. Both compounds are agonists of the vitamin D receptor and mediate biological effects similar to 1,25-dihydroxyvitamin D3. The 25-amino derivative is the most potent one while being less calcemic than 1,25-dihydroxyvitamin D3. The in vivo properties of these compounds make them potentially valuable for therapeutic purposes.
BIOORGANIC CHEMISTRY
(2023)
Correction
Biochemistry & Molecular Biology
Sunil Gaikwad, Carmen M. Gonzalez, Daniel Vilarino, Gonzalo Lasanta, Carmen Villaverde, Antonio Mourino, Lieve Verlinden, Annemieke Verstuyf, Carole Peluso-Iltis, Natacha Rochel, Klaudia Berkowska, Ewa Marcinkowska
BIOORGANIC CHEMISTRY
(2023)
Article
Endocrinology & Metabolism
Lieve Verlinden, Stefanie Doms, Iris Janssens, Mark B. B. Meyer, J. Wesley Pike, Geert Carmeliet, Annemieke Verstuyf
Summary: Neuropilin 2 (NRP2) plays a role in bone homeostasis by regulating osteoblasts and osteoclasts. The expression of NRP2 in osteoblasts is induced by 1,25-dihydroxyvitamin D-3. Deletion of NRP2 in osteoblasts results in reduced bone mass, while deletion in osteoclasts does not have a bone phenotype.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anna Y. Belorusova, Daniela Rovito, Yassmine Chebaro, Stefanie Doms, Lieve Verlinden, Annemieke Verstuyf, Daniel Metzger, Natacha Rochel, Gilles Laverny
Summary: This study demonstrates that BXL-62 and Gemini-72, two C-20-modified vitamin D analogs, restore the transcriptional activities of VDR variants unresponsive to the natural ligand. The elucidated mechanisms of action highlight the mutual adaptation between the ligand and the VDR ligand-binding pocket.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Catarina Meng, Hanne Skou Jorgensen, Lieve Verlinden, Nathalie Bravenboer, Henriette de Loor, Patrick C. D'Haese, Geert Carmeliet, Pieter Evenepoel
Summary: Kidney transplantation has limited impact on bone microarchitecture, with only slight decrease in trabecular thickness and stable parameters of bone volume and microarchitecture.
Article
Respiratory System
Jef Serre, Carolien Mathyssen, Tom Tanjeko Ajime, Tobias Heigl, Lieve Verlinden, Karen Maes, Annemieke Verstuyf, Didier Cataldo, Jeroen Vanoirbeek, Bart Vanaudenaerde, Wim Janssens, Ghislaine Gayan-Ramirez
Summary: Local delivery of vitamin D to the lungs efficiently reduced inflammatory cells induced by LPS in bronchoalveolar lavage and slightly attenuated an increase in CXCL5. In cases of severe vitamin D deficiency, despite not significantly minimizing cellular inflammation, local vitamin D prevented epithelial barrier leakage and damage in the lung. Additional research is needed to determine the long-term beneficial effects of local vitamin D delivery on lung inflammation.
RESPIRATORY RESEARCH
(2022)
