Journal
DEVELOPMENT
Volume 148, Issue 4, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.195511
Keywords
Basement membrane; ColIV; Morphogenesis; Cell intercalation
Categories
Funding
- Agence Nationale de la Recherche [ANR-13-BSV2-0006, ANR-17-CE13-0024]
- LABEX SIGNALIFE [ANR-11-LABX-0028-01]
- Universite Cote d'Azur
- Centre National pour la Recherche Scientifique (CNRS)
- Institut National de la Sante et de la Recherche Medicale (INSERM)
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Basement membranes (BM) are essential for organ morphogenesis and function, with Collagen type IV (ColIV) playing a key role in the assembly and integrity of the BM in Drosophila ovarian stalk morphogenesis. The dynamic assembly of ColIV in the stalk is crucial for maintaining ovarian integrity and preventing follicle fusion, highlighting the importance of BM in controlling tissue morphogenesis and integrity.
Basement membranes (BM) are extracellular matrices assembled into complex and highly organized networks essential for organ morphogenesis and function. However, little is known about the tissue origin of BM components and their dynamics in vivo. Here, we unravel the assembly and role of the BM main component, Collagen type IV (ColIV), in Drosophila ovarian stalk morphogenesis. Stalks are short strings of cells assembled through cell intercalation that link adjacent follicles and maintain ovarian integrity. We show that stalk ColIV has multiple origins and is assembled following a regulated pattern leading to a unique BM organisation. Absence of ColIV leads to follicle fusion, as observed upon ablation of stalk cells. ColIV and integrins are both required to trigger cell intercalation and maintain mechanically strong cell-cell attachment within the stalk. These results show how the dynamic assembly of a mosaic BM controls complex tissue morphogenesis and integrity.
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