Magnetic nanoparticle-decorated graphene oxide-chitosan composite as an efficient nanocarrier for protein delivery

Protein therapy has received significant importance in healthcare industry to combat a wide variety of diseases such as cancer, metabolic disorders, and autoimmune disease. However, the efficiency of in vitro and in vivo protein delivery is hindered by their protein instability and less life span. Herein, we prepared magnetic nanoparticles decorated with graphene oxide chitosan composite (Fe-GO-CS) as a nanocarrier for protein. Bovine serum albumin (BSA) is employed as a protein with the prepared nanocarrier to study its stability and activity. The structural and morphological analysis were carried out using XRD, FTIR, FESEM, VSM and Raman spectroscopy. SDS page analysis showed no remarkable change after exposing Fe-GO-BSA and Fe-GO-CS-BSA solution in trypsin after a time duration of 30 min and 3 h. The Fe-GO-CS exhibits a good drug loading and releasing profile when compared with Fe-GO composite and this nanocarrier protects the protein from enzymatic cleavage. Hence Fe-GO-CS composite is a better nanocarrier that can be applied for clinical applications practically.

Automated summary

In this study, magnetic nanoparticles decorated with graphene oxide chitosan composite were prepared as a nanocarrier for protein delivery, aiming to improve protein stability. Structural and morphological analysis revealed that this nanocarrier effectively protected the protein from enzymatic cleavage, making it a promising candidate for clinical applications.