Journal
CELLULAR SIGNALLING
Volume 79, Issue -, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2020.109878
Keywords
BC; circ_0069094; miR-591; HK2; Glycolysis
Categories
Funding
- Basic Scientific Research Operating Expenses of Central Universities [3332020026]
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The study found that circ_0069094 was upregulated in BC, associated with poor prognosis. Silencing circ_0069094 induced apoptosis, reduced proliferation, and glycolysis, while overexpression had opposite effects. Circ_0069094 was verified to regulate HK2 expression by sponging miR-591. These results suggest that circ_0069094 might serve as a prognostic biomarker and therapeutic target for BC.
Circular RNAs (circRNAs) are implicated in the initiation and advancement of diverse tumors. CircRNA hsa_circ_0069094 (circ_0069094) has been reported to be upregulated in BC, but the biological role of circ_0069094 in BC is indistinct. Hence, we aimed to survey the biological role of circ_0069094 in BC. In the present study, we verified that circ_0069094 was upregulated in BC tissues and cells. BC patients with high circ_0069094 expression had a poor prognosis. Functional analysis revealed that circ_0069094 silencing induced apoptosis, curbed proliferation, and reduced glycolysis in BC cells in vitro, but circ_0069094 overexpression had an opposing influence. Also, circ_0069094 knockdown reduced BC growth in vivo. Mechanically, circ_0069094 was validated as a decoy for miR-591, which targeted HK2. Importantly, circ_0069094 sponged miR-591 to regulate HK2 expression. Both miR-591 silencing and HK2 overexpression counteracted circ_0069094 inhibition-mediated influence on cell proliferation, apoptosis, and glycolysis in BC cells. In conclusion, these results indicated that circ_0069094 facilitated cell malignancy and glycolysis by upregulating HK2 through adsorbing miR591, suggesting that circ_0069094 might be a prognostic biomarker and therapeutic target for BC.
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