Journal
ACTA PHYSIOLOGIAE PLANTARUM
Volume 37, Issue 2, Pages -Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11738-014-1758-7
Keywords
Carbon sources; Nitrogen sources; Shoot production; Withanolides; Withania somnifera
Categories
Funding
- University Grants Commissions (UGC), Government of India
- Council of Scientific and Industrial Research (CSIR), Government of India
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Effect of nitrogen and carbon sources on multiple shoot regeneration and withanolides contents of Withania somnifera were evaluated. Inclusion of L-glutamine (20 mg/l) in medium fortified with the optimal levels of 1.5 mg/l 6-benzyladenine (BA) and 0.3 mg/l indole-3acetic acid (IAA) resulted in the production of 58 shoots/ explant. Sucrose at 4 % and 20 mg/l L-glutamine with ide eal concentrations of BA and IAA improved shoot multiplication (62 shoots/explant) while 6 % sucrose enhanced withanolides contents in regenerated multiple shoots. All the shoots were rooted (26 roots/shoot) when cultured in MS medium amended with 15 mg/l ammonium nitrate, 2 mg/l IBA, and 2 % sucrose. The present study resulted in the production of 4.42-fold higher multiple shoots and 2.6fold higher roots as well as enhancing the contents of all major withanolides (withaferin A, withanone, withanolide A and B) in regenerated plants when compared to previous reports on W. somnifera regeneration.
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