Article
Nutrition & Dietetics
Sehar Iqbal, Waqas Ahmed, Saira Zafar, Umar Farooq, Juweria Abid, Hassan Bin Usman Shah, Sajeela Akram, Madiha Ghazanfar, Abdul Momin Rizwan Ahmad
Summary: The study shows that prebiotic fortification combined with iron salts can significantly improve iron biomarkers and hematological indices in reproductive-age women with iron deficiency anemia.
FRONTIERS IN NUTRITION
(2022)
Review
Food Science & Technology
Michael W. Dougherty, Christian Jobin
Summary: Colibactin, a secondary metabolite encoded by the pks gene island in pathogenic Escherichia coli, may cause DNA damage and tumorigenesis. However, fundamental knowledge about colibactin's function is still lacking, and further research is needed to elucidate the biological mechanisms governing production and delivery of this compound by these bacteria.
Article
Multidisciplinary Sciences
Habiba Tariq, Zobia Noreen, Aftab Ahmad, Laraib Khan, Mashhood Ali, Muhammad Malik, Aneela Javed, Faisal Rasheed, Alina Fatima, Tanil Kocagoz, Ugur Sezerman, Habib Bokhari
Summary: This study investigated the genetic diversity of pathogenic E. coli strains associated with colorectal cancer (CRC). It found that 43.47% of the cancer-associated isolates belonged to phylogroup B2, showed resistance to multiple antibiotics, and exhibited cytotoxic activity that may contribute to the progression of CRC.
Review
Biochemistry & Molecular Biology
Camille V. Chagneau, Delphine Payros, Min Tang-Fichaux, Frederic Auvray, Jean-Philippe Nougayrede, Eric Oswald
Summary: In this review, the available literature is summarized to address questions regarding the function and presence of the biosynthetic gene cluster coding for colibactin, as well as its distribution in commensal and probiotic strains of Escherichia coli and other bacterial species.
TRENDS IN MICROBIOLOGY
(2022)
Review
Oncology
Ya Wang, Kai Fu
Summary: Colorectal cancer (CRC) is a major cause of cancer-related death worldwide, with an increasing incidence in developing areas. Bacterial pathogens from the gut microbiota, particularly Escherichia coli (E. coli), are recognized as significant risk factors for CRC. This review provides an update on the knowledge of E. coli-produced genotoxins associated with CRC. It discusses the various genotoxins produced by E. coli, their mechanisms of action, and their potential to induce tumorigenesis through DNA damage and cellular responses.
Review
Food Science & Technology
Thaisa Agrizzi Verediano, Nikita Agarwal, Mariana Juste Contin Gomes, Hercia Stampini Duarte Martino, Elad Tako
Summary: A systematic review on the effects of dietary fiber on iron absorption and iron status-related biomarkers found no significant correlations between the consumption of dietary fiber and iron absorption/biomarkers. However, the current evidence may not be substantial enough to invalidate the recommendation of dietary fiber as an agent to improve dietary iron bioavailability. Further clinical trials focusing on populations at high risk for iron deficiency are needed.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Microbiology
Manon Oliero, Roy Hajjar, Thibault Cuisiniere, Gabriela Fragoso, Annie Calve, Manuela M. Santos
Summary: Inulin has shown conflicting effects on colorectal cancer (CRC), with both protective and tumor-promoting effects observed. This study investigated the impact of colibactin-producing Escherichia coli on the protective effect of inulin against CRC. The results suggest that inulin supplementation may promote tumor growth and progression in mice colonized with colibactin-producing E. coli, indicating the importance of considering the presence of these bacteria when recommending inulin supplementation.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Gastroenterology & Hepatology
Michael W. Dougherty, Christian Jobin
Summary: The development of colorectal cancer is influenced by the presence of bacterial communities in the gastrointestinal tract. These microorganisms obtain essential nutrients from indigestible compounds and play a role in molecular signaling pathways that are necessary for normal tissue and immune function. Several bacterial species, such as Fusobacterium nucleatum, Enterotoxigenic Bacteroides fragilis, and pks(+) E. coli, have been identified to potentially increase the risk of colorectal cancer. Recent research has expanded on this topic to include host mutational status, microbial heterogeneity within tumors, transient infections, and the cumulative effects of multiple carcinogenic bacteria. This review provides an updated overview of how host-bacteria interactions influence colorectal cancer development, potential applications in diagnosis or prevention, and the impact of the gut microbiome on colorectal cancer treatment efficacy.
Article
Biology
Kristyna Mezerova, Lubomir Stary, Pavel Zboril, Ivo Klementa, Martin Stasek, Petr Spicka, Pavel Skalicky, Vladislav Raclavsky
Summary: The frequent occurrence of cyclomodulin-positive E. coli in CRC patients provides the opportunity to use them as CRC screening markers; a significant association was found between CRC and the presence of colibactin genes clbB and clbN, the cnf gene, and hemolytic phenotype in E. coli isolates; colibactin gene assay may serve as an auxiliary marker to increase the specificity and/or sensitivity of FIT in CRC screening.
Article
Cell Biology
Jennifer C. Hallam, Sofia Sandalli, Iris Floria, Natasha C. A. Turner, Min Tang-Fichaux, Eric Oswald, Nicky O'Boyle, Andrew J. Roe
Summary: In this study, the inhibitory effect of D-Serine on the expression of the pks island in colibactin-producing strains was demonstrated. It was also found that D-Serine could reduce the cytopathic effects on host cells during infection with pks+ strains.
Article
Nutrition & Dietetics
Ambra Giorgetti, Daniela Paganini, Suzane Nyilima, Robert Kottler, Max Frick, Simon Karanja, Thierry Hennet, Michael B. Zimmermann
Summary: This study aimed to test whether the intake of 3.0 g of galacto-oligosaccharides (GOS) or human milk oligosaccharides (HMO) enhances iron absorption from iron-fortified maize porridge in partially breastfed Kenyan infants, and whether the maternal HMO profile modulates these effects. The results showed that the intake of GOS increased iron absorption by 78%, while the intake of HMO did not affect iron absorption. Therefore, this study suggests that 2'-fucosyllactose (2'-FL) and lacto-N-neotetraose (LNnT) do not have a physiological role in facilitating iron absorption during infancy.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2023)
Article
Multidisciplinary Sciences
Amina Iftekhar, Hilmar Berger, Nassim Bouznad, Julian Heuberger, Francesco Boccellato, Ulrich Dobrindt, Heiko Hermeking, Michael Sigal, Thomas F. Meyer
Summary: Genotoxic colibactin-producing pks+ Escherichia coli infection induces characteristics of colorectal cancer cells in organoids, including enhanced proliferation, Wnt independence, and impaired differentiation. Mutations in genes related to the p53 pathway contribute to Wnt-independence in these organoids, suggesting a functional interplay between p53 and Wnt pathways in promoting transformation. The basis of transformation in these organoids is proposed to be larger chromosomal alterations and aneuploidy, consistent with the early appearance of chromosomal instability in colorectal cancer.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Agustina Rodriguez-Sorrento, Lorena Castillejos, Paola Lopez-Colom, Gloria Cifuentes-Orjuela, Maria Rodriguez-Palmero, Jose Antonio Moreno-Munoz, Diana Luise, Paolo Trevisi, Susana Maria Martin-Orue
Summary: In this study, probiotic strains and prebiotic showed potential in combating ETEC F4 infection to a certain extent, but there was no synergistic effect observed when administered together in this animal model.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Nutrition & Dietetics
Nadja Mikulic, Mary A. Uyoga, Daniela Paganini, Edith Mwasi, Nicole U. Stoffel, Christophe Zeder, Simon Karanja, Michael B. Zimmermann
Summary: In Kenyan infants, adding GOS to a single iron-fortified maize porridge test meal did not significantly increase iron absorption, suggesting long-term feeding of GOS may be needed to enhance iron absorption at this age.
JOURNAL OF NUTRITION
(2021)
Article
Microbiology
Juraj Bosak, Darina Kohoutova, Matej Hrala, Jitka Krenova, Paula Moravkova, Stanislav Rejchrt, Jan Bures, David Smajs
Summary: This study aimed to characterize mucosal E. coli isolates from patients with colorectal neoplasia and healthy controls. Results showed that E. coli isolated from neoplasia patients carried a higher prevalence of virulence determinants compared to the control group. These findings suggest that E. coli strains isolated from patients with colorectal neoplasia possess several virulence factors, which could contribute to the development of neoplastic processes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shady Mansour Kamal, Annika Cimdins-Ahne, Changhan Lee, Fengyang Li, Alberto J. Martin-Rodriguez, Zaira Seferbekova, Robert Afasizhev, Haleluya Tesfaye Wami, Panagiotis Katikaridis, Lena Meins, Heinrich Luensdorf, Ulrich Dobrindt, Axel Mogk, Ute Romling
Summary: In this study, E. coli Fec10, a newly isolated human commensal strain, was characterized for the first time. It was found to have unique genomic features and carry a plasmid with important physiological functions. The thermotolerance of E. coli Fec10 was closely related to its ClpG(GI) disaggregase, which showed distinct biochemical characteristics compared to disaggregases in other bacteria.
MOLECULAR MICROBIOLOGY
(2021)
Article
Immunology
Philipp Schiller, Michael Knoedler, Petya Berger, Lilo Greune, Angelika Fruth, Alexander Mellmann, Petra Dersch, Michael Berger, Ulrich Dobrindt
Summary: The study found differences in adherence properties and pathogenicity between the EHEC outbreak strain O104:H4 and its close relative due to expression of different types of aggregative adherence fimbriae (AAF). Various types of AAF affect bacterial autoaggregation, biofilm formation, and host cell adherence.
Article
Medicine, Research & Experimental
Ines Ambite, Nina A. Filenko, Elisabed Zaldastanishvili, Daniel S. C. Butler, Thi Hien Tran, Arunima Chaudhuri, Parisa Esmaeili, Shahram Ahmadi, Sanchari Paul, Bjorn Wullt, Johannes Putze, Swaine L. Chen, Ulrich Dobrindt, Catharina Svanborg
Summary: This study reveals a mechanism by which bacteria adapt to the host environment, modulating host gene expression by inhibiting the activity of RNA polymerase II in host cells, thus affecting the interaction between host and bacteria. By investigating the role of bacterial NlpD protein in hosts, new insights and possibilities for developing therapeutic approaches have been provided.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Chemistry, Multidisciplinary
Andrea Sowa, Alexander Hoeing, Ulrich Dobrindt, Shirley K. Knauer, Anzhela Galstyan, Jens Voskuhl
Summary: This study describes the synthesis, characterization, and application of water-soluble zinc(II) phthalocyanines decorated with umbelliferone moieties for photodynamic therapy. These compounds exhibited excellent phototoxicity and antimicrobial activity in cells and bacteria, making them promising photosensitizers for future broad applications.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Neurosciences
Yvonne Huisman, Katharina Uphoff, Michael Berger, Ulrich Dobrindt, Mario Schelhaas, Thomas Zobel, Jeroen Bussmann, Andreas van Impel, Stefan Schulte-Merker
Summary: Brain lymphatic endothelial cells (BLECs) in zebrafish have been found to efficiently uptake extracellular cargo molecules compared to microglia. While BLECs are effective in uptaking proteins, polysaccharides, and virus particles, microglia are better at ingesting larger particles like bacteria, indicating a clear distribution of tasks between the two cell types. Additionally, BLECs show similar substrate acceptance as scavenger endothelial cells (SECs) of the cardinal vein, and cooperate with microglia to remove particle waste from the brain.
Article
Infectious Diseases
Ulrich Dobrindt, Haleluya T. Wami, Torsten Schmidt-Wieland, Daniela Bertsch, Klaus Oberdorfer, Herbert Hof
Summary: Nitroxoline remains effective against some multidrug resistant uropathogenic bacteria, particularly E. coli, suggesting it could be a potential alternative to cotrimoxazole for treating uncomplicated urinary tract infections.
Article
Microbiology
Noble Selasi Gati, Imke Johanna Temme, Barbara Middendorf-Bauchart, Alexander Kehl, Ulrich Dobrindt, Alexander Mellmann
Summary: Hybrid Shiga toxin-producing Escherichia coli strains are positioned between STEC and UPEC, causing both diarrhea and urinary tract infections, with potential to adapt to both intestinal and extraintestinal environments.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Immunology
Paulo A. Schueroff, Fabia A. Salvador, Cecilia M. Abe, Haleluya T. Wami, Eneas Carvalho, Rodrigo T. Hernandes, Ulrich Dobrindt, Tania A. T. Gomes, Waldir P. Elias
Summary: The study revealed a hybrid strain UPEC-46 with characteristics of both EAEC and UPEC, with a significant role of AFP adhesin in adherence to colorectal and bladder cell lines.
Article
Biochemical Research Methods
Marla Keizers, Ulrich Dobrindt, Michael Berger
Summary: Bacteria use interspecies signaling molecules to control gene expression for the benefit of the bacterial community. The analysis and quantification of the signaling molecule AI-2 are important for understanding changes in bacterial behavior. Current bioassays for AI-2 are complex and sensitive, so researchers have developed a sensitive and reliable Escherichia coli biosensor-based assay for AI-2 quantification.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biology
Krishnendu Mukherjee, Ulrich Dobrindt
Summary: This article reviews the contributions of chromatin remodeling to insect immunity and adaptation to pathogens, focusing on the roles of DNA methylation, histone acetylation, and microRNAs in the evolution of insect resistance.
CURRENT OPINION IN INSECT SCIENCE
(2022)
Article
Evolutionary Biology
Nicolas C. H. Schroeder, Ana Korsa, Haleluya Wami, Olena Mantel, Ulrich Dobrindt, Joachim Kurtz
Summary: This study investigated the persistence and evolution of the probiotic Escherichia coli strain Nissle 1917 in an insect host and flour environment through experimental evolution. The findings suggest that EcN remains stable with weak phenotypic changes during serial passage in the insect host, indicating a certain degree of phenotypic plasticity.
EVOLUTION MEDICINE AND PUBLIC HEALTH
(2022)
Article
Toxicology
Robert Landsiedel, Daniela Hahn, Rainer Ossig, Sabrina Ritz, Lydia Sauer, Roland Buesen, Sascha Rehm, Wendel Wohlleben, Sibylle Groeters, Volker Strauss, Saskia Sperber, Haleluya Wami, Ulrich Dobrindt, Karola Prior, Dag Harmsen, Bennard van Ravenzwaay, Juergen Schnekenburger
Summary: The analysis of intestinal microbiome and plasma metabolome can serve as an early and sensitive indicator of gut microbiome changes induced by orally administered nanoparticles; this will help to recognize potential adverse effects of these changes to the host.
PARTICLE AND FIBRE TOXICOLOGY
(2022)
Article
Immunology
Paulo A. Schueroff, Cecilia M. Abe, Jonatas W. Silva, Cideli de Paula Coelho, Fernanda B. Andrade, Rodrigo T. Hernandes, Ulrich Dobrindt, Tania A. T. Gomes, Waldir P. Elias
Summary: This study evaluated the involvement of AFP and other adhesins in the uropathogenicity and intestinal colonization of bacteria. The results showed a synergistic role of AFP and TIF in the adherence and colonization of intestinal and urinary epithelia.
Article
Microbiology
Lang Peng, Rexford Mawunyo Dumevi, Marco Chitto, Nadja Haarmann, Petya Berger, Gerald Koudelka, Herbert Schmidt, Alexander Mellmann, Ulrich Dobrindt, Michael Berger
Summary: This study proposes a new method for genetic manipulation of enterohemorrhagic Escherichia coli (EHEC) that is more efficient than traditional methods. The method increases recombineering efficiency by 3-4 times in EHEC O157:H7 EDL933 Delta stx1/2, but shows similar efficiency in Escherichia coli K-12 MG1655. Additionally, the study suggests that the inhibition of the RecFOR pathway indirectly blocks phage induction and improves recombineering efficiency.