Article
Biochemistry & Molecular Biology
Yalin Hu, Xin Lu, Liyue Shen, Jiahui Dong, Zhanwei Liang, Jie Xie, Tao Peng, Xiaoping Yu, Xinhua Dai
Summary: In this study, an immunochromatographic assay (ICA) based on magnetic nanoparticles (MNPs) was successfully developed for the sensitive detection of carcinoembryonic antigen (CEA). The developed method shows excellent performance, sensitivity, and efficiency, providing technical support for cancer diagnosis and prognosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Thais S. Correa, Anamelia L. Bocca, Florencio Figueiredo, Emilia C. O. Lima, Maria De Fatima M. Almeida Santos, Zulmira G. M. Lacava, Mariana Campos-da-Paz
Summary: Systemic therapy is essential for breast cancer treatment, but side effects and resistance are concerns, especially for TNBC. New nanoformulations targeting cancer cells and alternative biomarkers for TNBC management have been explored. In vivo study showed MFCEA nanoparticles effectively targeted CEA-positive TNBC tumors in mice, with promising potential for clinical application. Further research is needed to improve performance and ensure safety for future studies.
BIOMEDICAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Yanmin Zhang, Chenfang Sun, Yuchen Duan, Shanshan Cheng, Wenping Hu
Summary: This study presents the design and fabrication of a carbon dots-functionalized organic field effect transistor for biosensing applications. By utilizing the advantages of carbon dots, low-abundance proteins were successfully detected while maintaining device performance.The experimental results demonstrate that this biosensor exhibits an extremely low limit of detection and high selectivity, highlighting its potential for early cancer detection.
Article
Chemistry, Physical
Sami Pekdemir, Hasan Hueseyin Ipekci, Murat Serhatlioglu, Caglar Elbuken, M. Serdar Onses
Summary: This manuscript presents a novel approach for fabrication of microscopic linear barcodes that can be visualized under Raman microscopy, using microfluidic channels and colloidal gold nanoparticles to generate plasmonic arrays and unique Raman fingerprints. The method offers advantages in size, complexity, and anti-counterfeiting, and can be easily adapted to different colloidal nanomaterials and applications.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Analytical
Qinglei Shu, Yafei Zhu, Yipi Xiao, Kaisen Chen, Xi Mai, Xiangjuan Zheng, Xiluan Yan
Summary: The establishment of stable and simple detection methods for cancer markers is crucial for cancer treatment. In this study, dual aptamers for carcinoembryonic antigen (CEA) were used to specifically recognize CEA and form a sandwich structure. Two different strategies, one using magnetic beads and nickel hexacyanoferrate nanoparticles (Ni NPs) and the other using streptavidin-modified gold nanoparticles (Au NPs), were employed to achieve signal cascade amplification. The detection limits of the two methods were very low, allowing for the detection of protein biomarkers at extremely low concentrations in a small amount of serum, which has great significance for the early diagnosis of cancer.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Chaoqun Wang, Ziqiang Deng, Hu Zhang, Rui Liu, Yi Lv
Summary: Magnetic particles (MPs) are widely used in various biological applications, and this study discovered a novel function of commercialized MPs-internal standard for accurate quantification of biomolecules. The proposed MPs-based immunoassay greatly improves the accuracy and precision by eliminating potential MPs loss and intensity fluctuations during magnetic separation.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Analytical
Yingzhou Tao, Weihao Shi, Fang Luo, Bin Qiu, Zhenyu Lin
Summary: Gold nanoparticles exhibit strong photothermal effect after aggregation induced by cysteine and are inhibited by copper ions, enabling detection of Cu2+ and development of a convenient photothermal immunoassay for cancer biomarker CEA.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Pengli Li, Min Wang, Mingzhe Jiang, Wenjing Lai, Jiajia Li, Kailin Liu, Hongling Li, Chenglin Hong
Summary: The sensitive detection of carcinoembryonic antigen (CEA) was achieved using an electrochemical immunosensor loaded with gold nanoparticles and flower-shaped CuS nanoflowers with peroxidase-like activity. This immunosensor exhibited excellent analytical performance for the quantitative detection of CEA.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Materials Science, Biomaterials
Eun Young Hwang, Jae Hee Lee, Dong Woo Lim
Summary: In this study, bimetallic nanorod clusters-poly(aniline) nanocomposites with gold nanorod clusters were synthesized and applied for SERS-based biosensing of CEA. The GNRCs in SBS or ETE configurations showed strong electromagnetic field enhancement, with the ETE configuration controlled by temperature-triggered hydration-dehydration of PAAc-b-PNIPAM chains. The distinct surface functionalities of JRCPCs enabled quantitative analysis of CEA using SERS nanoprobes, demonstrating the potential for advanced Janus nanocomposites in SERS-based biosensing applications.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Chemistry, Analytical
Xuefang Gu, Kaiyue Wang, Jiawei Qiu, Yajie Wang, Shu Tian, Zhenkuan He, Ran Zong, Heinz-Bernhard Kraatz
Summary: A dual readout biosensor combining electrochemical and SERS techniques was developed for multiplexed immunoassay of tumor biomarkers. The biosensor showed high sensitivity and unique charge selectivity, suitable for differential pulse voltammetry and square wave voltammetry detection. The hybridization chain reaction increased the load of labeled molecules, and the biosensor achieved high-throughput screening of alpha-fetoprotein and carcinoembryonic antigen with wide concentration ranges and low detection limits.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Cristina B. Adamo, Ronei J. Poppi, Dosil P. de Jesus
Summary: This work shows that depositing a Ni-P film on a PDMS substrate can enhance the magnetic aggregation of gold-modified magnetic nanoparticles, improving their performance in surface-enhanced Raman scattering detection. By combining the PDMS-Ni-P substrate with a permanent NdFeB magnet, the aggregation of Fe3O4/AuNPs is improved, leading to significantly higher SERS signals for crystal violet, adenine, and thiabendazole.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Physical
Debashree Das, Shalmali Basu, Santanu Ray, Sudeep Koppayithodi, Bibhas Hazra, Subhajit Bandyopadhyay, Abhijit Saha, Kamalika Sen
Summary: A simple and rapid analytical method using selenium nanoparticles for the detection of colorectal cancer biomarker CEA has been reported. The sensitivity of CEA detection was enhanced by gamma irradiation of the nanoparticles, and the interference from comorbidity-causing biomolecules was investigated.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Biophysics
Kang Shao, Wuyan Xie, Qingqing Ling, Tao Wang, Hongxi Zhang, Yuanjie Teng, Shiyi Ye, Xiufang Yuan, Zaifa Pan
Summary: A tri-modal sensing platform based on dumbbell-like upconversion nanoparticles and G-quadruplex DNAzyme was developed for accurate quantitative analysis of carcinoembryonic antigen (CEA) in a wide range. The platform utilizes upconversion luminescence, photothermal, and catalysis signal readouts for sensitive detection. This tri-modal sensing method shows a wide linear range and low limit of detection, making it suitable for analysis of various clinical samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Engineering, Biomedical
Francisca Diniz, Maria Azevedo, Flavia Sousa, Hugo Osorio, Diana Campos, Paula Sampaio, Joana Gomes, Bruno Sarmento, Celso A. Reis
Summary: Drug delivery using nanoparticles has the potential for cancer therapy, particularly for gastric cancer. This study developed surface-functionalized nanoparticles with specific antibodies, which effectively recognized cancer cells expressing the target antigen under flow conditions. These findings highlight the potential use of such nanoparticles for drug delivery in cancer.
MATERIALS TODAY BIO
(2022)
Article
Chemistry, Analytical
Zaifa Pan, Di Yang, Jing Lin, Kang Shao, Shuxian Shi, Yuan-Jie Teng, Huijun Liu, Yuanbin She
Summary: Carcinoembryonic antigen (CEA) is an important biomarker for cancer diagnosis. A novel fluorescence aptasensor was designed using near-infrared persistent luminescence nanoparticles (PLNPs) for accurate detection of CEA in pleural effusion. The sensor effectively eliminates the interference from autofluorescence and has high accuracy and low detection limit.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Jiu Deng, Xiuli Zhang, Zongzheng Chen, Yong Luo, Yao Lu, Tingjiao Liu, Zhengzhi Wu, Yu Jin, Weijie Zhao, Bingcheng Lin
Article
Multidisciplinary Sciences
Yahui Ji, Dongyuan Qi, Linmei Li, Haoran Su, Xiaojie Li, Yong Luo, Bo Sun, Fuyin Zhang, Bingcheng Lin, Tingjiao Liu, Yao Lu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Chemistry, Analytical
Nayi Wang, Yao Lu, Zhuo Chen, Rong Fan
Article
Chemistry, Analytical
Zongzheng Chen, Jiu Deng, Xiuli Zhang, Yong Luo, Yao Lu, Zhengzhi Wu, Bingcheng Lin
ANALYTICA CHIMICA ACTA
(2020)
Article
Biochemical Research Methods
Meimei Liu, Meihua Jin, Linmei Li, Yahui Ji, Fengjiao Zhu, Yong Luo, Tingjiao Liu, Bingcheng Lin, Yao Lu
Review
Chemistry, Analytical
Ye Cong, Xiahe Han, Youping Wang, Zongzheng Chen, Yao Lu, Tingjiao Liu, Zhengzhi Wu, Yu Jin, Yong Luo, Xiuli Zhang
Article
Engineering, Biomedical
Shiqiang Yan, Shuting Wang, Zhujing Hao, Meimei Liu, Chunyue Miao, Md Fazle Alam, Ruihan Bai, Linmei Li, Yong Luo, Tingjiao Liu, Bingcheng Lin, Weijia Zhang, Yao Lu
Summary: The study presents a convenient method for rapid fabrication of PDMS microdevices on non-planar surfaces, using a flexible PVC mask to achieve micro-patterning of HL/HB interfaces. This method is simple and efficient, requiring low input of time and toxic chemicals. Additionally, the flexible mask has been shown to facilitate cell patterning on curved substrates, providing a useful tool for PDMS prototyping and cell patterning.
Article
Chemistry, Analytical
Linmei Li, Weiwei Shi, Meimei Liu, Xue Bai, Yanting Sun, Xue Zhu, Haoran Su, Yahui Ji, Fengjiao Zhu, Xianming Liu, Yong Luo, Tingjiao Liu, Bingcheng Lin, Yao Lu
Summary: This study introduces a single-cell cytokine secretion analysis platform that integrates three-dimensional cell culture and primary oral squamous cell carcinoma tumor cell co-culture, which allows for the investigation of immune responses of human macrophages stimulated with lipopolysaccharide. The differential modulation effect of the tumor microenvironment on cytokine secretions was observed, with TNF-a secretion attenuated and IL-6 increased. The findings highlight the importance of the cell microenvironment in single-cell analysis and its impact on immune cell responses.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Fengjiao Zhu, Yahui Ji, Linmei Li, Xue Bai, Xianming Liu, Yong Luo, Tingjiao Liu, Bingcheng Lin, Yao Lu
Summary: Researchers have developed a high-throughput single-cell EV secretion analysis platform using a combination of gold nanoparticle-enhanced silver staining and the Poisson distribution, which can be operated with a home scanner without the need for cell counting. This platform allows for the analysis of different EV phenotypes and the quantification of EV secretion from each individual cell, providing a simple, easily accessible, and affordable tool for both basic and clinical research.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yufang Liu, Jiao Li, Jiasheng Zhou, Xue Liu, Huibing Li, Yao Lu, Bingcheng Lin, Xiaojie Li, Tingjiao Liu
Summary: Angiogenesis was assessed using a microfluidic chip, showing VEGF as a key determinant for vascular sprouting initiation. Different isoforms of VEGF displayed varying effects on sprout length and number, vessel perfusion, and connectivity, with VEGF(165) demonstrating the highest capacity to induce vascular sprouting and functional vessel formation.
Article
Chemistry, Analytical
Cuiyun Jia, Bin Li, Jie Pan, Fengjiao Zhu, Xue Bai, Xianming Liu, Bingcheng Lin, Yahong Chen, Yao Lu
Summary: Inspired by Chinese bamboo braiding art, we developed a novel fabrication technique using PDMS-impregnated paper substrate to create three-dimensional paper-based microfluidic devices. These devices offer advantages such as compactness, easy disassembly, and real-time flow visualization, making them suitable for various applications.
ANALYSIS & SENSING
(2023)
Article
Nanoscience & Nanotechnology
Fengjiao Zhu, Songnan Liu, Xue Bai, Xianming Liu, Bingcheng Lin, Yao Lu
Summary: In this study, a portable and low-cost desktop scanner was proposed for the detection of multiplexed proteins secreted from single cells. Gold nanoparticle-enhanced silver staining and tyramide signal amplification were combined to construct a two-step amplification for the detection of single-cell secretion assays. The developed platform eliminates bulky equipment for single-cell secretion analysis, making it more accessible for basic and clinical research.
Article
Biochemical Research Methods
Yaru Xing, Yu Liu, Rifei Chen, Yuyan Li, Chengzhi Zhang, Youwei Jiang, Yao Lu, Bingcheng Lin, Peizhong Chen, Ruijun Tian, Xianming Liu, Xing Cheng
Summary: This study addresses the scalability and reliability issues of low-cost printed circuit board digital microfluidic platforms by using active-matrix circuitry. The reliable actuation of aqueous and organic droplets using a free-standing double-layer hydrophobic membrane demonstrates the versatility of the platform. By implementing these improvements, a fully automatic, scalable, robust, reusable, and low-cost digital microfluidic platform capable of parallel manipulation of a large number of droplets can be realized for various applications in chemical engineering, bioengineering, and biomedical engineering.
Article
Oncology
Jiao Li, Xue Liu, Shizhu Zang, Jiasheng Zhou, Fuyin Zhang, Bo Sun, Dongyuan Qi, Xiaojie Li, Jing Kong, Dong Jin, Xuesong Yang, Yong Luo, Yao Lu, Bingcheng Lin, Weidong Niu, Tingjiao Liu
Article
Engineering, Biomedical
Xiaorui Li, Quanfeng Deng, Tiantian Zhuang, Yao Lu, Tingjiao Liu, Weijie Zhao, Bingcheng Lin, Yong Luo, Xiuli Zhang
BIO-DESIGN AND MANUFACTURING
(2020)
