Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 166, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bej.2020.107846
Keywords
Biodegradable polymers; Tissue-engineered scaffolds; Retinal degenerative diseases; Regenerative medicine
Funding
- Royan Institute
- School of Engineering and Built Environment, Griffith University
Ask authors/readers for more resources
This study investigates the impact of different polyester-based biopolymer materials blended with PCL on the attachment and proliferation of RPCs, revealing that PGS/PCL scaffolds promote RPC attachment and proliferation more favorably compared to other blends, showing potential for future clinical therapy for retinal degenerative disorders.
Recent advances in cell transplantation technologies have shown that polymeric fibrous tissue-engineered scaffolds provide a suitable physical environment, including the structural support, for cell delivery and effectively mimic the transplanted cells' extracellular matrix. Our study investigates the structure, composition and properties of three most commonly used polyester-based biopolymer materials blended with poly(epsilon-caprolactone) (PCL) at 2:1 (wt.%) ratio, namely, poly(glycerol sebacate) (PGS)/PCL, polylactic-co-glycolic acid (PLGA)/PCL, poly-l-lactide (PLLA)/PCL and pure PCL as carrier vehicles for retinal progenitor cell (RPC) attachment and RPC proliferation. The physicochemical properties of PGS/PCL, PLLA/PCL, PLGA/PCL and pure PCL fibrous scaffolds, fabricated under the identical electrospinning conditions, were analysed employing scanning electron microscopy, contact angle analysis, Raman spectroscopy, electrical and ionic conductivity measurements, and supplemented by an in-vitro RPC adhesion and proliferation studies. Our findings have shown that PGS/PCL scaffolds promote RPC attachment and RPC proliferation more favourably compared to other polymeric blends and pure PCL, owing to a combination of advantageous surface and bulk properties, overall demonstrating a potential for PGS/PCL blend to become a suitable vehicle for RPC delivery in a possible future clinical therapy for the treatment of retinal degenerative disorders.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available