Article
Immunology
Peng Chen, Mingming Zhang, Yichan Zhang, Jun Li, Xihe Wan, Tingli Lv, Yiyue Chen, Zhigang Zhao, Zhihao Ma, Zhu Zhu, Lihua Chen, Zhen Li, Zisheng Wang, Guo Qiao
Summary: This study analyzed the impact of Cyprinid herpesvirus 2 (CyHV-2) infection on the gut microbiota and metabolite composition of gibel carp. The results showed that CyHV-2 infection significantly altered the gut microbiota and metabolite profiles, leading to an increase in the opportunistic pathogen Aeromonas. Furthermore, modulation of tryptophan metabolism affected the abundance of Aeromonas, the transcription of antiviral cytokines, and the infection level of CyHV-2.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Zhao-Ran Dong, Qing-Jiang Mu, Wei-Guang Kong, Da-Cheng Qin, Yong Zhou, Xin-You Wang, Gao-Feng Cheng, Yang-Zhi Luo, Tao-Shan Ai, Zhen Xu
Summary: This study developed an oral vaccine against CyHV-2 using yeast cell surface display technology and demonstrated its protective efficacy in gibel carp. The vaccine group showed higher activities of immune-related enzymes and gene expression, and strong innate and adaptive immune responses were elicited in mucosal and systemic tissues after oral vaccination with the yeast vaccine.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Meizhen Su, Cuiyu Lu, Ruizhe Tang, Xiaomi Zhang, Liqun Lu
Summary: The study demonstrates that the plant alkaloid berberine can effectively inhibit the expression and replication of CyHV-2 in crucian carp through negative regulation of the NF-xB signaling pathway. This indicates that berberine has the potential to serve as a small molecular drug for controlling CyHV-2 infection in fish. The findings also show that berberine can attenuate inflammatory injury and viral load in CyHV-2-infected crucian carp cell lines in a dose-dependent manner.
Article
Immunology
Tingting Zhang, Yuchao Gu, Xiaohan Liu, Rui Yuan, Yang Zhou, Yaping Dai, Ping Fang, Yongjie Feng, Guangli Cao, Hui Chen, Renyu Xue, Xiaolong Hu, Chengliang Gong
Summary: Encapsulating vaccines into protein microcrystals for stable delivery is a promising approach, as demonstrated by this study focusing on vaccinating gibel carp against CyHV-2 using polyhedra. The results showed that the vaccine proteins encapsulated in polyhedra could be protected from degradation, and vaccinated fish exhibited increased antibody titers and survival rates following CyHV-2 challenge, suggesting a novel strategy for developing aquaculture microencapsulated vaccines.
Article
Microbiology
Jinwei Gao, Yiwen Hu, Min Xie, Hao Wu, Jiayu Wu, Bingwen Xi, Rui Song, Dongsheng Ou
Summary: Infection of gibel carp with Cyprinid herpesvirus II leads to disruptions in physiological metabolic processes, liver and kidney damage, and different spatiotemporal expression patterns of Toll-like receptor 2 and Toll-like receptor 9, ultimately stimulating an antiviral response.
Article
Fisheries
Guo Qiao, Yichan Zhang, Jiwu Zhang, Wei Chen, Chang Wei, Peng Chen, Wenping Yang, Zhu Zhu, Mingming Zhang, Zhigang Zhao, Xihe Wan
Summary: Supplementation of poly-beta-hydroxybutyrate (PHB) can enhance the disease resistance and antioxidant capacity of gibel carps against CyHV-2 infection, inhibit viral proliferation in the gill, and improve the immune response. These findings are of great significance for developing novel prevention strategies to control CyHV-2 infection.
AQUACULTURE RESEARCH
(2023)
Article
Fisheries
Arathi Dharmaratnam, Arun Sudhagar, Thangaraj Raja Swaminathan
Summary: In this experiment, a heat-inactivated CyHV-2 vaccine was developed and evaluated in goldfish. The vaccine showed significant upregulation of immune genes and provided better protection against CyHV-2 in the challenged fish.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Veterinary Sciences
Qi Shan, Heqing Huang, Guangming Zheng, Yi Yin, Xinping Zhu, Lisha Ma, Hao Zhou, Wenping Xie, Lichun Li, Shugui Liu, Jingxin Wang
Summary: This study investigated the pharmacokinetics, tissue distribution, and elimination of enrofloxacin and its metabolite ciprofloxacin in crucian carp. The results showed that enrofloxacin exhibited slow absorption, wide tissue distribution, and long elimination half-life in the fish. Multiple-dose administration resulted in significant accumulations of the drugs in the plasma and tissues.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Fisheries
Bing-Yao Sun, Hai-Yan Kou, Pan -Yang Jian, Ling-Jiao Kong, Jie Fang, Peng-Kun Meng, Kang Wu, Cai-Gen Yang, Gang Yang, Xue-Hong Song
Summary: This study aimed to develop a specific IgY against CyHV-2 infection in gibel carp. Specific IgY was generated by immunizing laying hens with a recombinant D4ORFs protein and processed into spray-dried egg powder. The results showed that gibel carp receiving D4ORFs IgY had higher survival rates and lower viral load, as well as improved health and enhanced antiviral defense.
Article
Immunology
Arathi Dharmaratnam, Arun Sudhagar, Sweta Das, Reshma Rajeev Nair, Sundar Raj Nithianantham, Prasannan Geetha Preena, Narendrakumar Lekshmi, Thangaraj Raja Swaminathan
Summary: This study focused on developing an inactivated vaccine for the goldfish hematopoietic necrosis viral disease (GHNVD) and examining its immunogenicity. The results showed that the formalin-inactivated vaccine triggered immune gene expression in goldfish and significantly improved survival rates in a virus challenge experiment.
MICROBIAL PATHOGENESIS
(2022)
Article
Fisheries
Chang Wei, Chaonan Xu, Yuyu Sun, Jinzhou Li, Motohiko Sano, Qiang Li
Summary: This study found that CyHV-2 can establish persistent or latent infection in asymptomatic farmed gibel carp, and different viral mRNA expression patterns were observed in different individuals. This indicates that apparently healthy gibel carp can also be a source of infection.
Article
Fisheries
Hong-jun Wu, Fan Liu, Hong-Yao Du, Yan-Ge He, Chuan-He Bao, Zhong Wei, Ruo-lin Zhu
Summary: A Carassius carassius gill cell line (CCG) was constructed and successfully passaged up to 90 passages, providing a useful tool for studying the propagation and pathogenesis of CyHV-2.
AQUACULTURE REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
Wanjie Cai, Lele Fu, Cui Liu, Linyue He, Haokun Liu, Dong Han, Xiaoming Zhu, Yunxia Yang, Junyan Jin, Shouqi Xie
Summary: This study investigated the effect of D-ribose on the flesh quality of gibel carp. The results showed that D-ribose supplementation improved the nutritional value, texture, and flavor of the fish. It enhanced the water-holding capacity and hardness of the flesh, facilitated the deposition of amino acids and fatty acids, and activated purine metabolism pathways.
Article
Fisheries
Lu Yu, Qikang Chen, Xin Chu, Yang Luo, Zizhao Feng, Liqun Lu, Ye Zhang, Dan Xu
Summary: In this study, miR-124 was identified as a negative regulator of ccBAX gene expression during CyHV-2 infection in silver crucian carp. The results showed that miR-124 significantly down-regulated the expression of the ccBAX gene in silver crucian carp, indicating a potential mechanism of regulation during viral infection.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Hui Yang, Qingsheng Cao, Zhu Zhu, Yangyang Cao, Tongwei Ji, Wenzhi Wei, Haiyong Zhao, Yingying Zhang
Summary: The study found that Bdellovibrio has a certain lysing effect on common aquatic pathogens and can significantly reduce the mortality rate of fish caused by infections. Additionally, Bdellovibrio may colonize in fish. These results provide a theoretical basis for the application of Bdellovibrio in aquaculture.