Journal
ANALYTICA CHIMICA ACTA
Volume 1147, Issue -, Pages 170-177Publisher
ELSEVIER
DOI: 10.1016/j.aca.2020.12.050
Keywords
Pb2+ detection; Fluorescence resonance energy transfer (FRET); Tetrahedral DNA nanostructure (TDN); DNAzyme; Ratiometric detection; Biosensing
Categories
Funding
- Agricultural Science and Technology Innovation Program of CAAS [CAAS-ZDRW202011, GJFP2019.36]
- Fundamental Research Funds for Innovation Project [AEPI-004]
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A fluorescent sensing strategy using Pb2+ DNAzyme-controlled tetrahedral DNA nanostructure (TDN)-mediated hyper-branched hybridization chain reaction (hHCR) has been developed for rapid, highly sensitive, and specific detection of lead (II) ion. The method demonstrates ultrahigh sensitivity, strong anti-interference capacity, and short detection time, showing great potential for practical applications.
A fluorescent sensing strategy was developed for rapid, highly sensitive and specific detection of lead (II) ion (Pb2+) on the basis of Pb2+ DNAzyme-controlled tetrahedral DNA nanostructure (TDN)-mediated hyper-branched hybridization chain reaction (hHCR). In this strategy, DNA hairpins used for HCR amplification are modified on the four vertexes of TDN, which are then used to perform rapid TDN-hHCR in the presence of an initiator strand, producing large-sized cross-linked reaction products and thus giving greatly improved fluorescence resonance energy transfer (FRET) signal output. Pb2+ DNAzyme catalyzes the cleavage of the initiator strand, inhibiting the initiation of TDN-hHCR and giving decreased FRET signal. Synergetic signal amplification of Pb2+ DNAzyme-catalyzed cleavage reaction and subsequent TDN-hHCR confers the sensing platform with ultrahigh sensitivity. As low as 0.25 pM Pb2+ can be detected by using either signal turn-on or turn-off mode. The whole detection process can be finished within 20 min. Strong anti-interference capacity of FRET-based ratiometric detection and high specificity of Pb2+ DNAzyme endow the sensing platform with great practical application potential, which was demonstrated by the accurate detection of Pb2+ in real river water, fruit, vegetable and grain samples. (C) 2021 Elsevier B.V. All rights reserved.
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