Review
Biotechnology & Applied Microbiology
Pan Jin, Lei Liu, Xichi Chen, Lin Cheng, Weining Zhang, Gang Zhong
Summary: Hydrogels are emerging as promising biomaterials for meniscus rehabilitation and regeneration due to their tunability and ability to deliver exogenous substances. However, there are still challenges to be addressed in their clinical application.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Mithun Santra, Yu-Chi Liu, Vishal Jhanji, Gary Hin-Fai Yam
Summary: Corneal transparency is crucial for vision, but corneal opacity can cause blindness. Conventional corneal transplantation is limited by a lack of donor tissue, making bioengineered corneal tissues an emerging solution. Engineering with extracellular matrix (ECM) scaffolds, particularly using ultra-thin stromal laminar tissues obtained from refractive correction procedures, offers a promising approach for corneal regeneration and restoration of transparency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Gizem Zihna, Bengisu Topuz, Gulcin Gunal, Halil Murat Aydin
Summary: Acellular hybrid scaffolds consisting of in situ cross-linkable 3-D interpenetrating network structures were obtained by decellularization of meniscus tissues followed by integration of the gel system. These scaffolds showed tunable physicochemical and mechanical properties and could be used in meniscus tissue engineering.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2023)
Article
Biotechnology & Applied Microbiology
Hao Li, Zhiyao Liao, Zhen Yang, Cangjian Gao, Liwei Fu, Pinxue Li, Tianyuan Zhao, Fuyang Cao, Wei Chen, Zhiguo Yuan, Xiang Sui, Shuyun Liu, Quanyi Guo
Summary: This study presents a composite scaffold for meniscus tissue engineering, consisting of a 3D-printed PCL scaffold, injected MECM, and KGN-loaded PLGA microspheres. The combination showed enhanced hydrophilicity, bioactivity, cell adhesion, proliferation, and chondrogenic differentiation. The sustained release of KGN from the scaffold contributed to improved cell affinity and chondrogenic activity, indicating promising application prospects in MTE.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Francisco Drusso Martinez-Garcia, Martine Margaretha Valk, Prashant Kumar Sharma, Janette Kay Burgess, Martin Conrad Harmsen
Summary: The study determined the viscoelastic transformation of GelMA hydrogels caused by ASCs through mathematical modelling. Different concentrations of GelMA led to changes in stiffness and the number of Maxwell elements influenced by ASCs. Overall, 5% GelMA induced the most favorable response from ASCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Zhou Jian, Tian Zhuang, Tian Qinyu, Peng Liqing, Li Kun, Luo Xujiang, Wang Diaodiao, Yang Zhen, Jiang Shuangpeng, Sui Xiang, Huang Jingxiang, Liu Shuyun, Hao Libo, Tang Peifu, Yao Qi, Guo Quany
Summary: By optimizing the preparation of meniscus-derived bioink and utilizing a custom 3D bioprinting system, a biomimetic meniscal scaffold with optimal features in terms of morphology and components was successfully created. Comprehensive evaluations were conducted on cell viability, mechanics, biodegradation, and tissue formation to ensure the scaffold's feasibility and functionality for application in tissue engineering.
BIOACTIVE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Marta Redondo, Rafaela Presa, Pedro L. Granja, Marco Araujo, Aureliana Sousa
Summary: This study successfully developed degradable Konjac glucomannan (KGM) hydrogels for 3D cell culture and achieved cell-material interactions by modifying and grafting bioactive peptides into KGM. The results show great potential for the further application of KGM in tissue engineering.
MATERIALS TODAY CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Izabela Gabriela Rodrigues da Silva, Bruna Tassia dos Santos Pantoja, Gustavo Henrique Dona Rodrigues Almeida, Ana Claudia Oliveira Carreira, Maria Angelica Miglino
Summary: Cardiovascular diseases are the leading cause of death worldwide and are also common in dogs and cats. Conventional treatments cannot heal injured myocardium. Despite advances in cardiac therapy, transplantation is still considered the best treatment for heart diseases in humans. In veterinary medicine, the goal is to control symptoms and delay disease progression to improve the quality of life, although transplantation is ideal.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell & Tissue Engineering
Wenhui Zhang, Aoling Du, Shun Liu, Mingyue Lv, Shenghua Chen
Summary: dECM and hydrogels are widely used in regenerative medicine as biocompatible materials with unique biological activity. Hydrogels, with their high water content and ability to simulate human tissue environments, are considered ideal materials for tissue engineering.
REGENERATIVE THERAPY
(2021)
Review
Nanoscience & Nanotechnology
Xueqian Wang, Chuanliang Feng
Summary: Tissue engineering aims to develop biological substitutes for repairing damaged tissues and organs. Chiral supramolecular hydrogels have higher application potential in tissue engineering due to their ability to mimic the natural extracellular matrix under physiological conditions and regulate tissue formation through their chiral characteristics. This review highlights the importance of chiral supramolecular hydrogels in tissue engineering, discusses the considerations for their design and the influence of external stimuli on their construction, and summarizes their potential application prospects and the contribution made by our group in this field.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Shengran Wang, Changlai Zhu, Bin Zhang, Junxia Hu, Jinghui Xu, Chengbin Xue, Shuangxi Bao, Xiaokun Gu, Fei Ding, Yumin Yang, Xiaosong Gu, Yun Gu
Summary: A favorable microenvironment is crucial for nerve regeneration. This study investigates the components and spatial organization of extracellular matrix (ECM) secreted by different cells and finds that ECM derived from bone marrow mesenchymal cells (BMSCs) is more similar to acellular nerves. ECM-modified nerve grafts (ECM-NGs) co-cultivated with BMSCs exhibit the most promising nerve repair properties and create a superior microenvironment for nerve regeneration, indicating a potential alternative for clinical repair of peripheral nerve defects.
Review
Materials Science, Multidisciplinary
Shunli Wu, Xianmin Wu, Xiuhui Wang, Jiacan Su
Summary: Bone organoids offer realistic models for bone regenerative medicine development, and the matrix microenvironment around tissues and cells plays a crucial role. Matrigel, traditionally used as a matrix, faces challenges, making newly developed hydrogels suitable alternatives. This review summarizes the development and limitations of Matrigel and highlights promising hydrogel alternatives for bone organoid cultures.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Silvia Vieira, Joana Silva-Correia, Rui L. Reis, J. Miguel Oliveira
Summary: Hydrogels are versatile platforms in tissue engineering with a wide range of applications. Their properties can be tuned through various methods, making them valuable for drug and cell-based therapies as well as fundamental research. The diversity in sources, crosslinking strategies, functionalization methods, and similarity to the extracellular matrix make hydrogels an excellent tool in tissue engineering. This review provides an overview of hydrogel design, processing methods, and the impact of different formulations on cell-biomaterial interactions.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Engineering, Biomedical
Jorke Willemse, Gilles van Tienderen, Eline van Hengel, Ivo Schurink, Diana van der Ven, Yik Kan, Petra de Ruiter, Oskar Rosmark, Gunilla Westergren-Thorsson, Kerstin Schneeberger, Bram van der Eerden, Henk Roest, Bart Spee, Luc van der Laan, Jeroen de Jonge, Monique Verstegen
Summary: This study describes the culture and expansion of human cholangiocyte organoids using liver ECM-derived hydrogels. These hydrogels support the proliferation of organoids and maintain their phenotypic characteristics. Compared to traditional mouse tumor-derived extracellular matrix, liver ECM hydrogels do not significantly alter gene or protein expression and are not affected by species-specific differences. Additionally, the use of human liver ECM extracts enables the expansion of organoids in a dynamic culture set up without the need for laborious static culture.
Article
Engineering, Biomedical
Tsong-Hann Yu, Tsu-Te Yeh, Chen-Ying Su, Ni-Yin Yu, I-Cheng Chen, Hsu-Wei Fang
Summary: This study prepared acellular cartilage matrix (ACM) powder through decellularization method and developed ACM hydrogels. The results showed that ACM hydrogels had good gel conditions, retained main components of cartilage, and exhibited good gelation and adhesive properties.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Fei Chen, Gernot Hochleitner, Tim Woodfield, Juergen Groll, Paul D. Dalton, Brian G. Amsden
Article
Polymer Science
Julian P. Chesterman, Fei Chen, Amanda J. Brissenden, Brian G. Amsden
Article
Materials Science, Biomaterials
Dimitra A. Louka, Nathan Holwell, Brandon H. Thomas, Fei Chen, Brian G. Amsden
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2018)
Article
Materials Science, Biomaterials
Dimitra A. Louka, Nathan Holwell, Brandon H. Thomas, Fei Chen, Brian G. Amsden
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2018)
Article
Engineering, Biomedical
Gernot Hochleitner, Fei Chen, Carina Blum, Paul D. Dalton, Brian Amsden, Juergen Groll
ACTA BIOMATERIALIA
(2018)
Article
Polymer Science
Fei Chen, Ronghui Qi, Locke Davenport Huyer, Brian G. Amsden
POLYMER DEGRADATION AND STABILITY
(2018)
Article
Medicine, Research & Experimental
Sara Mohajeri, Fei Chen, Mitchell de Prinse, Ta Phung, Jonah Burke-Kleinman, Donald H. Maurice, Brian G. Amsden
MOLECULAR PHARMACEUTICS
(2020)
Review
Cell & Tissue Engineering
Meng Wang, Yixuan Luo, Yin Yu, Fei Chen
STEM CELLS INTERNATIONAL
(2020)
Article
Engineering, Biomedical
Ran Yang, Fei Chen, Jinshan Guo, Dongfang Zhou, Shifang Luan
BIOACTIVE MATERIALS
(2020)
Article
Pharmacology & Pharmacy
Gang Zhong, Huiping Long, Fei Chen, Yin Yu
Summary: In this study, oxoglaucine was found to attenuate progression of deterioration and pathological changes in osteoarthritis by activating autophagy and inhibiting calcium influx. It suppressed expression of proinflammatory and apoptosis-related proteins, prevented matrix degradation in OA chondrocytes, and blocked Ca2+ influx while up-regulating autophagic markers. Oxoglaucine shows potential as a candidate drug for OA treatment.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Engineering, Biomedical
Gang Zhong, Huiping Long, Tian Zhou, Yisi Liu, Jianping Zhao, Jinyu Han, Xiaohu Yang, Yin Yu, Fei Chen, Shengliang Shi
Summary: In this study, the authors developed Prussian blue/polyamidoamine (PAMAM) dendrimer/Angiopep-2 (PPA) nanoparticles for the treatment of Alzheimer's disease (AD). These nanoparticles exhibited superior blood-brain barrier permeability and had synergistic effects on scavenging ROS and restoring mitochondrial function of microglia. The nanoparticles effectively reduced neurotoxic A beta aggregate and improved cognitive functions in the AD mouse model.
Article
Biochemistry & Molecular Biology
Meng Wang, Jianping Zhao, Yixuan Luo, Qianyi Liang, Yisi Liu, Gang Zhong, Yin Yu, Fei Chen
Summary: This study reports a method for fabricating mechanically tunable scaffolds for cartilage regeneration using 3D printing. The addition of short fibers improves printing fidelity and generates mechanically strong constructs. By optimizing the composition of the bioink, scaffolds with good biocompatibility and support for chondrogenesis can be achieved. This method has significant potential for various applications.
MACROMOLECULAR BIOSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Haiyuan Lv, Guotao Deng, Jiaqi Lai, Yin Yu, Fei Chen, Jun Yao
Summary: This review summarizes the current bioprinting techniques employed in developing engineered meniscus grafts and discusses the latest strategies for mimicking the gradient structure, composition, and viscoelastic properties of native meniscus. Recent progress in gene-activated matrices for meniscus regeneration is also highlighted. Finally, a perspective is provided on the future development of 3D bioprinting for meniscus repair, emphasizing the potential of this technology to revolutionize meniscus regeneration and improve patient outcomes.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Polymer Science
Yisi Liu, Meng Wang, Yixuan Luo, Qianyi Liang, Yin Yu, Fei Chen, Jun Yao
Summary: Stem cells hold promise for cartilage repair in osteoarthritis, but challenges in large-scale production, viability maintenance, and therapeutic activity hinder clinical application. Advanced hydrogel technologies, particularly microgels and dynamic hydrogel systems, show potential in improving stem cell survival and regulation of stem cell fate.
Article
Engineering, Biomedical
Jianqiu Yang, Zhenzhen Huang, Jiang Tan, Jingye Pan, Shixuan Chen, Wenbing Wan
Summary: By functionalizing a decellularized pomelo peel with an adhesive hydrogel and antibacterial materials, the hybrid wound dressing can effectively inhibit bacterial infection, promote granulation tissue formation and angiogenesis, and reduce scar formation during wound healing.
BIOACTIVE MATERIALS
(2024)
Article
Engineering, Biomedical
Yong Mei, Xiaohua Qin, Zhenyu Yang, Shiyao Song, Xiaoting Liu, Chong Wu, Jieying Qian, Xiaowan Huang, Yunjiao Zhang, Weiling He
Summary: This article presents a novel nanoparticle for treating KRAS-TP53 co-mutation in gastrointestinal cancers. The nanoparticle can degrade mutant p53 proteins (mutp53) and deliver the drug AMG510 to inhibit mutant KRAS and mutp53 signaling pathways. The results show that the nanoparticle can effectively reduce cell proliferation and migration, and demonstrate remarkable therapeutic efficacy in a tumor-bearing mouse model.
BIOACTIVE MATERIALS
(2024)