Article
Biotechnology & Applied Microbiology
Giannis Penloglou, Alexandros Pavlou, Costas Kiparissides
Summary: In this study, the production of polyhydroxybutyrate (PHB), a natural polyester, was achieved using Azohydromonas lata and cheese whey as a low-cost substrate. The efficiency of cheese whey as a carbon source for PHB production was found to be comparable to pure sugars. By using the Taguchi method and a dual optimization approach, two sets of conditions were identified to maximize the %PHB content and the weight average molecular weight of PHB. The results showed that the carbon-to-nitrogen ratio (C/N) was the most important parameter for the molecular weight, while the dissolved oxygen concentration (DO) had the most significant impact on the %PHB content.
FERMENTATION-BASEL
(2023)
Article
Chemistry, Physical
Raman Rao, Nitai Basak
Summary: In this study, pretreated cheese whey was used to produce hydrogen via dark fermentation by Enterobacter aerogenes 2822 cells. The effects of total carbohydrate concentration, temperature, and pH on volumetric hydrogen production rate were investigated, with an optimum rate achieved at specific conditions. Mathematical models were developed to describe the kinetics of substrate consumption and growth profile, contributing to the conversion of cheese whey into a clean form of bioenergy.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Energy & Fuels
Rovsen Guliyev, Mustafa Akgun, Bilge Sayin Borekci, Omer Sadak, Yusuf Esen
Summary: This study aimed to treat cheese whey using magnetic nanoparticles and optimize the process parameters. The results showed the optimal conditions for nitrogen content, nitrogen removal rate, chemical oxygen demand, COD removal rate, nitrite content, and nitrite removal rate. This study is significant for reducing the environmental impact of whey.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biochemistry & Molecular Biology
Yuan Li, Xiyu Tang, Liangqi Chen, Wenhui Zhu, Weidong Huang, Jinyao Li
Summary: This study successfully generated variants of nattokinase with enhanced fibrinolytic activity and stability by mutating the surface amino acid residues. Bioinformatics analysis revealed that the increased activities of these variants were related to the increased flexibility of the region around their active centers. These findings provide potential methods for industrial production and application of nattokinase.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Erica Pontonio, Marco Montemurro, Gina Valeria De Gennaro, Valerio Miceli, Carlo Giuseppe Rizzello
Summary: A biotechnological protocol was developed to obtain bioactive peptides with ACE-inhibitory activity from the ricotta cheese exhausted whey (RCEW) by combining membrane filtration and fermentation. The fermented protein-rich retentate showed high anti-ACE activity, with identified peptides overlapping with previously reported antihypertensive fragments. The fortified ricotta cheese with fermented retentate exhibited significantly higher anti-ACE activity and improved sensory properties compared to the control and unfermented samples.
Review
Food Science & Technology
Danfeng Li, Lizhen Hou, Miao Hu, Yaxin Gao, Zhiliang Tian, Bei Fan, Shuying Li, Fengzhong Wang
Summary: This article discusses the role of nattokinase (NK) in cardiovascular diseases and its production methods. NK, as the most bioactive ingredient in natto, has potential beneficial cardiovascular effects. Microbial synthesis is an economical method for NK production, but yield, activity and stability remain limitations. These issues can be addressed through various optimization strategies.
Review
Food Science & Technology
Arona Figueroa Pires, Natali Garcia Marnotes, Olga Diaz Rubio, Angel Cobos Garcia, Carlos Dias Pereira
Summary: The search for new food products that promote consumers health is ongoing, with cheese whey (CW) and second cheese whey (SCW) proving to contain potential ingredients for the development of functional food products. While whey products have gained popularity for their nutritional quality, the by-products CW and SCW are often disregarded or used for animal feed, leading to environmental concerns. More research is needed on the nutritional and functional properties of SCW in order to find ways to reuse this by-product and reduce environmental impacts.
Editorial Material
Food Science & Technology
Francisco J. Barba
Summary: The valorization of whey involves integrating different processes into a single process to achieve complete recovery, including physical-chemical processes and fermentation. Innovative processing technologies can also be used to reduce microbial load in cheese whey and improve the quality and safety of the final products.
Article
Agriculture, Dairy & Animal Science
E. Reale, S. Govindasamy-Lucey, Y. Lu, M. E. Johnson, J. J. Jaeggi, M. Molitor, J. A. Lucey
Summary: An increasing number of cheese-making companies are using high-protein milks to increase cheese yield. This study explored the impact of reducing whey protein (WP) levels in concentrated cheese milk on cheese characteristics. The results showed that reducing WP levels did not affect the pH, sensory attributes, or insoluble calcium content of cheese, but it did lower the protein hydrolysis and hardness and melting point of the cheese.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Arona F. Pires, Natali G. Marnotes, Amal Bella, Jorge Viegas, David M. Gomes, Marta H. F. Henriques, Carlos J. D. Pereira
Summary: The study demonstrated that whey cheeses produced from concentrated bovine whey with added cream showed lower protein content and higher levels of total solids and fat. The incorporation of Kefir and probiotics decreased the elastic modulus and viscosity of the samples, with the need for appropriate packaging solutions to control yeast and mold growth during storage.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Agriculture, Dairy & Animal Science
Ane Aldalur, Maria Angeles Bustamante, Jesus Salmeron, Luis Javier R. Barron
Summary: Different cutting and cooking processes have varying effects on the composition and properties of Idiazabal cheese. High-intensity processes lead to increased fat and casein losses, resulting in a more brittle and adhesive cheese, while moderate-intensity processes contribute to higher cheese yield.
JOURNAL OF DAIRY SCIENCE
(2021)
Review
Food Science & Technology
Afirah Mohd Shukri, Abdul Karim Alias, Maizura Murad, Kin-Sam Yen, Lai-Hoong Cheng
Summary: Cheese is in high demand due to its convenience and taste, but the manufacturing process is time-consuming and expensive, and it contains high saturated fat which is linked to health risks. Therefore, there is a shift towards developing plant-based imitation cheese products to meet market demands.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2022)
Article
Engineering, Chemical
Hilario Flores-Mejia, Antonio Lara-Musule, Eliseo Hernandez-Martinez, Ricardo Aguilar-Lopez, Hector Puebla
Summary: This paper addresses the issue of efficient monitoring in anaerobic digestion processes, presenting two indirect monitoring approaches and discussing their performance using experimental data. Fractal analysis plays a key role in the data-driven approach, while observer techniques are shown to accurately predict key process variables.
Article
Environmental Sciences
A. Polettini, R. Pomi, A. Rossi, T. Zonfa, G. De Gioannis, A. Muntoni
Summary: Despite being widely investigated, the production of hydrogen through dark fermentative processes using organic residues still faces challenges that limit its implementation on a larger scale. This study used continuous fermentation experiments with synthetic cheese whey as the substrate to gain detailed understanding of the process and identify suitable operating conditions. The results showed that the main features of the hydrogenogenic fermentation process can be described using a reduced set of factors, providing valuable insights for process monitoring and prediction.
Article
Microbiology
Reinhard Pinontoan, Elvina, Astia Sanjaya, Juandy Jo
Summary: The study found that the Indonesian commercialized B. subtilis G8 has fibrinolytic activity, able to dissolve blood clots and containing multiple fibrinolytic enzymes. Compared to nattokinase, B. subtilis G8 demonstrated potent fibrinolytic activity.
BIOSCIENCE OF MICROBIOTA FOOD AND HEALTH
(2021)
Article
Agricultural Engineering
Anshu Priya, Shifa Naseem, Deepshikha Pandey, Anisha Bhowmick, Mustafa Attrah, Kasturi Dutta, Eldon R. Rene, Sunil Kumar Suman, Achlesh Daverey
Summary: The utilization of algal biomass for biohydrogen production faces challenges due to the complex cell wall composition, requiring additional pretreatment. Conventional pretreatment strategies have limited feasibility and economic viability. This review discusses the structural complexities of algal cell walls and highlights innovative approaches such as hybrid technologies, biosurfactants, nanoparticles, and genetic engineering for improved hydrolysis and biohydrogen production. It also provides a comprehensive evaluation of various pretreatment methods and techno-economic and life cycle assessment of algal biohydrogen production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Dibyajyoti Haldar, Asma Musfira Shabbirahmed, Biswanath Mahanty
Summary: In this study, multivariate regression and artificial neural network (ANN) models were developed using curated experimental data to predict reducing sugar generation from lignocellulosic biomass (LCB). The results showed that acid and enzyme had a positive influence on reducing sugar generation, while treatment severity, temperature, and time had a negative influence. The genetic algorithm-optimized ANN model performed well in predicting LCB hydrolysis, with high fitness on training, validation, and test sets. Sensitivity analysis revealed that lignin and hemicellulose contents could be inhibitory factors. Although polynomial models offer simple interpretation, optimized ANN models provide better predictability for datasets with diverse biomass compositions.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pema Lhamo, Biswanath Mahanty
Summary: The increasing trend in bioplastic application has led to the emergence of polyhydroxyalkanoates (PHAs) as a promising candidate due to their bio-origin, biodegradability, and biocompatibility. This study investigates the effect of acetic acid concentration and its time of addition on PHA production by Cupriavidus necator. The addition of 1 g l(-1) acetic acid positively influenced biomass and PHA yield, but further increases had a reverse effect. Adding acetic acid during incubation resulted in higher PHA yield, while maximum biomass was achieved when acetic acid was added after 48 hours. Genetic algorithm-optimized artificial neural network (GA-ANN) modeling showed superior fitness compared to the polynomial model, and the optimized ANN model projected a PHA concentration of 2.691 g l(-1) with the addition of acetic acid during incubation.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ankita Priyadarshini, Soumya Mishra, Naresh Kumar Sahoo, Sangeeta Raut, Achlesh Daverey, Bankim Chandra Tripathy
Summary: The biodegradation of phenol using an indigenous Rhodococcus pyridinivorans strain PDB9T NS-1 encapsulated in a calcium alginate bead system was investigated. Experimental conditions, such as contact time, pH, and initial phenol doses, were studied and the results showed more than 99.6% degradation of phenol at an initial dose of 1000 mg/L in 70 hours at 30 degrees C. The immobilized microbe exhibited superior phenol biosorption capacity and exceptional operating stability.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Priyanka Agarwal, Rutuja Vibhandik, Roma Agrahari, Achlesh Daverey, Radha Rani
Summary: Due to extensive industrialization and escalation in pollution, phytoremediation using plants and plant secretions has gained attention for remediation of heavy metal-contaminated soil. Plant root exudates act as an ecological driver in the rhizospheric region, influencing microbial community and bioavailability of pollutants. This review focuses on the role of root exudates in phytoremediation of heavy metal-contaminated soil, particularly lead, and their impact on the biogeochemistry of lead.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Shelly Verma, Achlesh Daverey
Summary: In this study, a sequencing batch reactor was used to activate the anammox process, and stable nitrogen removal was achieved at nitrogen loading rates ranging from 20 to 116 (g N m(-3) d(-1)).
GEOMICROBIOLOGY JOURNAL
(2023)
Review
Engineering, Environmental
Pema Lhamo, Biswanath Mahanty
Summary: Microbially synthesized polyhydroxyalkanoates are being explored as an eco-friendly alternative to conventional plastic, with Poly(3-hydroxybutyrate-co-hydroxy valerate) (PHBV) copolymer showing potential for varied material properties. Strategies such as the inclusion of volatile fatty acid precursors, nutrient-rich organic waste utilization, and metabolic engineering have expanded the scope of sustainable PHBV production. This review provides insight into manipulating PHBV quality and quantity in microbiological processes, as well as blending with other biopolymers for applications in tissue engineering, drug delivery, and food packaging.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2023)
Article
Environmental Sciences
Biswanath Mahanty, Pema Lhamo, Naresh K. Sahoo
Summary: The formalization of a stable water quality index (WQI) is crucial for water resource identification and classification. The PCA-based WQI approach, which derives parameter weight from PC loading or rotated factor loading, has inherent limitations and can result in rating disagreements. This study modeled the WQI of groundwater samples using PCA-based approaches and found bias and disagreement in the ratings. Repeated resampling and cross-validation techniques were used to assess the stability of PCA-based WQI.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Vijendra Shah, Pooja Dani, Achlesh Daverey
Summary: This study investigates the impact of biosurfactant on the removal of cadmium from soil and finds that an appropriate concentration of biosurfactant can promote plant growth and increase enzymatic activities in soil.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pema Lhamo, Biswanath Mahanty
Summary: Mathematical modeling is important for optimal design of bioprocess in microbial polyhydroxyalkanoates (PHA) production. However, the selection of kinetics and model identification strategies from experimental data in PHA production remains largely heuristic. In this study, a parametric discretization approach was used to model PHA production from Cupriavidus necator using sucrose and urea. Logistic growth and Luedeking-Piret model were selected for PHA production based on regression coefficient (R-2: 0.941), adjusted R-2 (0.930), and AICc values (-42.764). The modeling framework can be used to avoid over-parameterization and identifiability issues and design optimal batch startup policies.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Biswanath Mahanty
Summary: Hybrid modeling is increasingly used in bioprocess modeling, experimental design, parameter identification, and optimization. However, developing a hybrid model from experimental data is a complex process involving designed experiments, selection of data-driven processes, model parameter identification, fitness assessment, and generalization capability. The challenges and solutions in hybrid-model development were reviewed, along with the lack of data and code sharing hindering the exploration and expansion of tools in the bioprocess system.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Environmental Sciences
Harini Methma Perera, Anushka Upamali Rajapaksha, Sudantha Liyanage, Anusha Ekanayake, Rangabhashiyam Selvasembian, Achlesh Daverey, Meththika Vithanage
Summary: A novel Chitosan-modified Mimosa pigra biochar (CMPBC) was found to have higher efficiency in removing hexavalent chromium (Cr(VI)) from aqueous systems compared to pristine biochar. The sorption of Cr(VI) was heavily dependent on pH, with the highest adsorption occurring at pH 3.0. The results suggest the potential use of CMPBC as an easily available, environmentally sustainable, and inexpensive sorbent for the decontamination of Cr(VI) from water.
ENVIRONMENTAL RESEARCH
(2023)
Article
Engineering, Environmental
Priyanka Verma, Achlesh Daverey, Kusum Arunachalam
Summary: Microbial fuel cells (MFCs) have the potential to treat wastewater and recover energy. The proton exchange membrane (PEM) is a crucial component of MFCs, affecting its performance and cost. This study developed a low-cost membrane from forestry waste pine needles, by preparing pine needle biochar through pyrolysis and subsequent sulphonation. The resulting membrane, consisting of sulphonated pine needle biochar (SPB) mixed with montmorillonite clay (MMT), showed improved water uptake capacity and achieved high removal efficiency of chemical oxygen demand in MFCs, suggesting its viability as an affordable option for bioelectricity production.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Engineering, Environmental
Deepshikha Pandey, Achlesh Daverey, Kasturi Dutta, Kusum Arunachalam
Summary: Textile industries produce wastewater with harmful azo dyes that are known to be carcinogenic and mutagenic. This study evaluates the potential use of enzyme immobilized biochar for removing azo dyes from real and simulated textile effluents. The results show that the enzyme immobilized system can achieve high removal efficiency for individual azo dyes in the simulated effluent and for one dye peak in the real effluent. The presence of metabolite in the real effluent indicates the degradation of the dye. Therefore, enzyme immobilized biochar system can be used for simultaneous adsorption and degradation of toxic azo dyes from real effluents.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Environmental Sciences
Qammer Zaib, Sangchul Park, Shishir Kumar Behera, Biswanath Mahanty, Mohd. Zafar, Hung-suck Park, Daeseung Kyung
Summary: This study aims to use waste materials as eco-friendly alternatives to coal in power plants. The study investigates the effect of waste material composition on energy value and ash fusion temperature, and develops a quadratic model for optimization.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Engineering, Environmental
Francisco de Borja Ojembarrena, Elena Fuente, Angeles Blanco, Carlos Negro
Summary: Accumulation of Cr(VI) in industrial fiber cement process water can affect product quality and health, and it needs to be removed to avoid environmental risks. The study found that cationic cellulose nanocrystals (CCNCs) have higher adsorption efficiency and lower cost compared to conventional adsorbents.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)
Review
Engineering, Environmental
Shentan Liu, Yangchen Zhang, Xiaojuan Feng, Sang-Hyun Pyo
Summary: This paper summarizes the common problems of constructed wetlands (CWs) and proposes corresponding optimized solutions, including thermal insulation and additional aeration in cold climates, choosing suitable plant species and planting patterns to enhance phytoremediation in CWs, using various methods to monitor and evaluate CW clogging, implementing anti-clogging measures, controlling greenhouse gas emissions during CW treatment, constructing and utilizing CW databases, designing appropriate CW types, and implementing strict technical management and supervision.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)
Article
Engineering, Environmental
Norzila Mohd, Nazlina Haiza Mohd Yasin, Wan Hasnidah Wan Osman, Mohd Sobri Takriff
Summary: This study demonstrates that Chlamydomonas sp. is effective in removing nutrients from palm oil mill effluent and shows particular efficiency in the removal of ammonium and phosphate.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)
Article
Engineering, Environmental
Mingzhen Zhang, Guijian Liu, Ruijia Liu, Jinzhao Xu, Wen Si, Yong Wei
Summary: Tieguanyin tea stem was used to synthesize modified biochar for the adsorption of copper and zinc. Among the modified biochar, Fe-BCs had the highest adsorption capacity, with Fe-BC650 being the best adsorbent. It reached adsorption equilibrium in 1 minute and had a maximum adsorption capacity of 303.5996 mg/g for Cu (II) and 254.0711 mg/g for Zn (II) in single-metal solutions. However, there were antagonistic impacts between Cu2+ and Zn2+.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)
Article
Engineering, Environmental
Lingling Zhao, Qiaoshu Zhou, Yun Yang, Yuan Zhang, Yewei Qiu, Yanjun Chen, Xian Jin, Xiangjun Yang, Shixiong Wang
Summary: This research presents a method for selectively recovering gold from e-waste water using metal sulfides microspheres prepared by hydrothermal technique. The results show that the assistance of visible light significantly improves the gold adsorption performance of metal sulfides. The metal sulfides exhibit excellent gold adsorption properties, high selectivity, ultra-high adsorption capacity, and good recyclability.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)
Article
Engineering, Environmental
Laishram Saya, W. Rameshwor Singh, Sunita Hooda
Summary: Organic-inorganic nanoblends, such as polysaccharide-blended-inorganic ones, have versatile applications and have gained significant research attention. In this study, a magnetic guar gum/graphene oxide nanocomposite blended with CuO was successfully prepared and investigated for its adsorption capacity for ciprofloxacin removal. The results showed that the nanocomposite exhibited excellent adsorption capacity and the adsorption process was spontaneous.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)
Article
Engineering, Environmental
Umit Ecer, Sakir Yilmaz
Summary: A novel and effective catalyst Co@MIL-68(Fe)@Fe3O4@CM-BC was synthesized and used for the catalytic reduction of crystal violet in aqueous environments. The catalyst showed high efficiency in degrading crystal violet under specific conditions, and exhibited good regeneration performance.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)
Article
Engineering, Environmental
Chi Zhang, Miao Zhang, Haohan Yang, Wei Cai, Jun Wu
Summary: Enhancing the bioaugmentation of common nitrifiers in the partial nitrification/anammox process can decrease the activity of nitrite oxidizing bacteria and increase the activity of anammox bacteria, leading to improved total nitrogen removal efficiency.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)
Article
Engineering, Environmental
Runzhang Zuo, Dajun Ren, Yangfan Deng, Canhui Song, Yubin Yu, Xiejuan Lu, Feixiang Zan, Xiaohui Wu
Summary: This study found that fine bubble aeration in membrane bioreactors (MBR) can better control membrane fouling and reduce energy consumption. However, compared to fine bubble aeration, coarse bubble aeration released more proteins and polysaccharides, resulting in a decrease in functional bacteria and enzymes involved in nitrogen and phosphorus pathways.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)
Article
Engineering, Environmental
Ch Tahir Mehmood, Jiekai Dai, Hanjun Zhao, Ziyi Zhong
Summary: Wirelessly powered photocatalyst-coated spheres (WPS) have been developed and demonstrated for various applications, including photodegradation and fouling control. These WPS are cost-effective, easy to fabricate, and have excellent visible light sources, making them suitable as carriers for photocatalysts.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)
Article
Engineering, Environmental
Yifan Wang, Xiaoxuan Kang, Yuting Li, Ruyi Li, Chang Wu, Luyao Wang, Chongqing Wang, Tao Yang, Ming Ge, Zhangxing He
Summary: Cobalt/carbon nanofibers (Co/CNF) were prepared and exhibited excellent catalytic activity in the degradation of tetracycline by activating peroxodisulfate (PDS). The effects of process parameters on tetracycline removal were studied, and the reaction mechanism was confirmed through experiments.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)
Article
Engineering, Environmental
Ting Wei, Yaqian Zhao, Jia Guo, Bin Ji, Alvaro Pun Garcia, Abraham Esteve Nunez
Summary: This study developed a novel lightweight substrate (Al-NLS) composed of aluminum sludge and polyurethane, and investigated its effectiveness in constructed wetlands (CWs). Results showed that Al-NLS had favorable adsorption capacities for phosphorus and removal efficiencies for pollutants in real domestic wastewater. It also positively influenced the microbial community. Therefore, Al-NLS can be considered as a new alternative substrate in CWs technology.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)
Article
Engineering, Environmental
Dabojani Das, Achinta Bordoloi, Heyang Yuan, Daniel J. Caldwell, Rominder P. S. Suri
Summary: This study evaluates the degradation kinetics of antibiotic resistance genes and mobile genetic elements in water matrices by ozone treatment. The results show that extracellular antibiotic resistance genes can be effectively removed, while intracellular genes are more resistant to degradation. The impact of wastewater matrix on gene degradation is minimal.
JOURNAL OF WATER PROCESS ENGINEERING
(2024)