Article
Plant Sciences
Qinmi Xie, Hui Hou, Peixuan Yan, Haiying Zhang, Yingze Lv, Xuebin Li, Lin Chen, Danbo Pang, Yang Hu, Xilu Ni
Summary: Aerenchyma formation in Nelumbo nucifera root is an adaptive trait to the aquatic environment, which involves programmed cell death. The study found that exogenous oxygen and ethylene can affect aerenchyma formation and its area, with ethylene having a positive regulatory effect. On the other hand, the inhibitor 1-MCP partially inhibits aerenchyma occurrence.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Kakali Bhadra
Summary: This article reviews the alternative pathways of cancer cell death targeted by anticancer drug molecules, providing a molecular basis for new strategies in cancer treatment. Many drugs can effectively induce cell death through targeting the caspase-independent cell death pathway, which is significant for cancer therapy.
Article
Gastroenterology & Hepatology
Ping Sun, Jie Zhong, Hong Liao, Patricia Loughran, Joud Mulla, Guang Fu, Da Tang, Jie Fan, Timothy R. Billiar, Wentao Gao, Melanie J. Scott
Summary: This study found that hepatocytes can activate pyroptosis pathways and cleave gasdermin-D (GSDMD), but unlike macrophages, pyroptotic hepatocytes do not exhibit significant cell rupture and morphological changes. These findings have important implications for understanding and recognizing the role of these pathways in hepatocytes, and may lead to the development of novel therapeutics for liver diseases.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Juan A. Montero, Carlos Ignacio Lorda-Diez, Juan M. Hurle
Summary: This paper proposes a new perspective on the regulation of programmed cell death (PCD) in developing systems based on epigenetics. It challenges the traditional view that PCD is solely controlled by specific gene-encoded signals, arguing that the signals and local tissue interactions responsible for embryonic tissue growth and differentiation play a significant role in PCD. The study suggests that cells retain an epigenetic profile sensitive to DNA damage, resulting in their subsequent elimination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Elham Memary, Alireza Imani, Ali Arhamidolatabadi, Parvaneh Fadavi, Marjan Aghajani, Farnoosh Mohebzadeh, Mohammad Shahverdi-Shahraki, Ali Dabbagh, Alirza Mirkheshti, Sadegh Shirian
Summary: This study investigated the effects of epinephrine (EP) alone and EP plus methylprednisolone (MP) on neurological outcomes after cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) in a rat model. The results showed that EP alone had the poorest neurological outcomes, while the combination of EP and MP protected neurons from death induced by hypoxia and reperfusion injury.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Veterinary Sciences
Raquel Ausejo, Juan Manuel Martinez, Noelia Mendoza, Alfonso Bolarin, M. Teresa Tejedor, Maria Victoria Falceto
Summary: This study aimed to compare different techniques for measuring sperm nuclear DNA fragmentation (sDF) and examine their relationship with boar reproductive value, classical spermiogram parameters, and reproductive results of the doses in sows. The findings indicate a significant correlation between sperm chromatin stability assay (SCSA) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay results. However, there was no correlation between sDF and fertility direct boar effect (DBE) index, total or progressive sperm motility. TUNEL measure showed a positive correlation with abnormal acrosomes and sperm chromatin dispersion test (SCD, Halomax (R)) measure showed a positive correlation with total sperm morphological abnormalities. No significant correlations were found between fertility or prolificacy results and sDF results with the different techniques.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
A. Dziedziech, U. Theopold
Summary: This study provides evidence for the ancient origin of pyroptosis in Drosophila melanogaster, showing that the inflammatory based programmed cell death mechanism fits the features of pyroptosis and relies on ancient apoptotic machinery.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Obstetrics & Gynecology
Manish Banker, Silke Dyer, Georgina M. Chambers, Osamu Ishihara, Markus Kupka, Jacques de Mouzon, Fernando Zegers-Hochschild, G. David Adamson
Summary: The report provides data on the utilization, effectiveness, and safety of assisted reproductive technology (ART) globally in 2013, showing an increase in ART cycles and changes in trends such as a rise in the proportion of older women undergoing non-donor ART. Further efforts are needed to improve the quality and consistency of reporting ART data by registries.
FERTILITY AND STERILITY
(2021)
Review
Biochemistry & Molecular Biology
Sung Un Huh
Summary: Plants have metaCaspases instead of caspases, which play critical roles in programmed cell death during plant development and defense responses. The diversity in the number of metaCaspases in different plant species suggests varying functions due to diverse evolutions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Plant Sciences
N. Tariq, M. Yaseen, D. Xu, H. M. Rehman, M. Bibi, M. Uzair
Summary: The tapetum is the innermost layer of the rice anther that plays a crucial role in the development of pollen grains. Mutation in PCD-associated genes can cause male sterility in rice. Recent research has focused on the genomic, transcriptomic and proteomic studies of tapetum development in rice, as well as its role in male reproductive development. This research will enhance our understanding of the tapetum's importance in male fertility and its applications in rice hybridization and other major crops.
Article
Obstetrics & Gynecology
Georgina M. Chambers, Silke Dyer, Fernando Zegers-Hochschild, Jacques de Mouzon, Osamu Ishihara, Manish Banker, Ragaa Mansour, Markus S. Kupka, G. David Adamson
Summary: In 2014, 1.93 million ART cycles were performed in 76 countries, with trends showing increased success rates, frozen embryo transfer cycles, single embryo transfer, and preimplantation genetic testing. Utilization rates and effectiveness of ART continue to improve globally.
HUMAN REPRODUCTION
(2021)
Article
Agronomy
Natthapong Janhom, Kanda Whangchai
Summary: The effect of ozone fumigation on the quality of 'Jinda' chili fruit was investigated in relation to ROS-mediated chlorophyll degradation and cell death-related processes. The results showed that ozone fumigation enhanced antioxidant activity, suppressed chlorophyll degradation and cell death, and delayed senescence in chili.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Susanne Vogeler, Stefano Carboni, Xiaoxu Li, Alyssa Joyce
Summary: This study identified multiple homologues of caspases in bivalves through bioinformatics and phylogenetic analyses, revealing novelty and complexity in the bivalve caspase family. The findings suggest unique roles of caspases in bivalve immune response and common diseases, providing essential new insights for further investigations.
Review
Biochemistry & Molecular Biology
Sihan Qi, Qilin Wang, Junyou Zhang, Qian Liu, Chunyan Li
Summary: Pyroptosis, a type of programmed cell death accompanied by inflammation, plays crucial roles in cancer initiation, progression, and immunotherapeutic outcome. Comparisons with other types of cell death help understand the unique characteristics of pyroptosis. Further research is needed to elucidate the molecular mechanisms between pyroptosis and cancer, and explore new therapeutic targets and strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yeram Choi, Sangkyu Park, Seul Lee, Ha-Eun Shin, Sangil Kwon, Jun-Kyu Choi, Myeong-Heon Lee, Seung-Yong Seo, Younghee Lee
Summary: This study investigated the anti-cancer effect of synthetic homoisoflavane derivatives on breast cancer cells and found that these compounds reduced cell proliferation through G2/M cell cycle arrest and induced caspase-independent cell death. They also downregulated heme levels, induced reactive oxygen species generation and lipid peroxidation, and reduced expression of glutathione peroxidase 4. Therefore, the accumulation of iron from heme degradation and ferroptosis might be potential mechanisms for caspase-independent cell death.
BIOMOLECULES & THERAPEUTICS
(2023)
Article
Agriculture, Dairy & Animal Science
Claudia Lima Verde Leal, Karina Canon-Beltran, Yulia N. Cajas, Meriem Hamdi, Aracelli Yaryes, Maria Gemma Millan de la Blanca, Paula Beltran-Brena, Rosane Mazzarella, Juliano Coelho da Silveira, Alfonso Gutierrez-Adan, Encina M. Gonzalez, Dimitrios Rizos
Summary: This study aimed to evaluate the effect of extracellular vesicles (EVs) isolated from oviductal fluid (OF) and uterine fluid (UF) on the development and quality of bovine embryos in vitro. The results showed that the use of EVs improved blastocyst quality, including survival rate after vitrification/warming, total cell number, lipid content, and the expression of lipid metabolism-related genes. Additionally, the miRNA contents in EVs may contribute to these observed effects.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Itishree Jali, Venkata Krishna Vanamamalai, Priyanka Garg, Paula Navarrete, Alfonso Gutierrez-Adan, Shailesh Sharma
Summary: This study analyzed RNA-seq data from bovine genital ridges of early embryonic stages and identified a large number of lncRNA transcripts. The study also investigated the co-expression between lncRNAs and the XIST gene, revealing a potential important role of differentially expressed lncRNAs during embryo development in bovine.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Reproductive Biology
Beatriz Galiano-Cogolludo, Pilar Marigorta, Victor Yus Giron, Pablo Bermejo-Alvarez, Priscila Ramos-Ibeas
Summary: The development failures occurring between blastocyst hatching and implantation in farm ungulates contribute significantly to pregnancy losses. The study focused on the post-hatching developmental period of the ovine embryo and investigated the impact of TGF13 inhibition on hypoblast and epiblast development. The findings revealed the negative effect of TGF13 inhibition, which could be partially reversed by ROCK inhibitor Y-27632, providing insights into the molecular networks regulating embryo development and the causes of early pregnancy losses in farm ungulates.
Article
Agriculture, Dairy & Animal Science
Sara Ruiz-Diaz, Rosane Mazzarella, Paula Navarrete-Lopez, Raul Fernandez-Gonzalez, Celia de Frutos, Maria Maroto, Claudia Cucala, Paula Beltran-Brena, Marta Lombo, Dimitrios Rizos, Alfonso Gutierrez-Adan
Summary: This study aimed to analyze if bull spermatozoa could be selected by thermotaxis and to assess their quality in terms of SDF as well as determine the presence of a specific sperm subpopulation based on sperm morphometry and assess their fertilizing capacity by ICSI. The results showed that thermotactic sperm selection led to higher DNA integrity, small and elongated head size parameters, and a different sperm subpopulation structure.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Developmental Biology
Paula Navarrete-Lopez, Marta Lombo, Maria Maroto, Eva Pericuesta, Raul Fernandez-Gonzalez, Priscila Ramos-Ibeas, Maria Teresa Parra, Alberto Viera, Jose Angel Suja, Alfonso Gutierrez-Adan
Summary: In this study, the role of the testis-specific IQ motif-containing H gene (Iqch) in the regulation of alternative splicing during spermatogenesis was characterized using a mutant mouse model. The findings suggested that Iqch is essential for the specific expression of RNA isoforms and interacts with other proteins to regulate alternative splicing events. The study also revealed the downregulation of testis-specific lncRNAs and protein-coding genes, as well as alterations in alternative splicing patterns, resulting in male infertility.
Article
Agriculture, Dairy & Animal Science
Isabel Gimeno, Pascal Salvetti, Susana Carrocera, Julie Gatien, Pablo Garcia-Manrique, Cristina Lopez-Hidalgo, Luis Valledor, Enrique Gomez
Summary: The selection of competent recipients prior to embryo transfer is crucial for improving pregnancy and birth rates in cattle. Ignoring the competence of the embryo can lead to failed pregnancy predictions. It is hypothesized that the pregnancy potential of biomarkers can be enhanced with information on embryonic competence.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Reproductive Biology
Karina Canon-Beltran, Yulia N. Cajas, Encina Gonzalez, Raul Fernandez-Gonzalez, Natacha Fierro, Pedro L. Lorenzo, Maria Arias-Alvarez, Rosa M. Garcia-Garcia, Alfonso Gutierrez-Adan, Dimitrios Rizos
Summary: Guinea pig in vitro fertilization (IVF) is underdeveloped due to limited oocyte accessibility and lack of efficient sperm capacitation methods. In this study, we evaluated different capacitation protocols and validated them through sperm analysis and heterologous IVF with bovine oocytes. Protocols C and D showed higher viability, motility, and acrosome reaction of spermatozoa compared to protocols A and B. Further analysis revealed that protocol D achieved higher pro-nuclear formation and cleavage rate in heterologous IVF, suggesting its suitability for guinea pig IVF.
Article
Immunology
Miguel Rodriguez-Pulido, Eva Calvo-Pinilla, Miryam Polo, Juan-Carlos Saiz, Raul Fernandez-Gonzalez, Eva Pericuesta, Alfonso Gutierrez-Adan, Francisco Sobrino, Miguel A. Martin-Acebes, Margarita Saiz
Summary: We evaluated the antiviral activity of synthetic RNAs mimicking specific domains in the non-coding regions of the foot-and-mouth disease virus (FMDV) genome (ncRNAs), which have shown broad-spectrum antiviral activity. The ncRNAs exhibited potent antiviral activity against SARS-CoV-2 in human intestinal and lung epithelium cells. In vivo efficacy studies using K18-hACE2 mice showed that administration of naked ncRNA before SARS-CoV-2 infection significantly reduced viral load and pro-inflammatory cytokines in the lungs. Furthermore, the ncRNAs were highly effective against other coronaviruses in human and swine cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Paula Navarrete-Lopez, Maria Maroto, Eva Pericuesta, Raul Fernandez-Gonzalez, Marta Lombo, Priscila Ramos-Ibeas, Alfonso Gutierrez-Adan
Summary: Kpna2 plays a crucial role in spermatogenesis. Knockout male mice lacking Kpna2 displayed reduced body size, decreased sperm motility, increased sperm abnormalities, dysregulated testis gene expression, and ultimately infertility. The impaired mRNA expression mainly affected gene clusters expressed in spermatids and spermatocytes, including genes involved in cell adhesion and extracellular matrix organization.
Article
Food Science & Technology
Ana Ruiz de la Bastida, Susana Langa, Angela Peiroten, Raul Fernandez-Gonzalez, Abel Sanchez-Jimenez, Maria Maroto, Jose Antonio Curiel, Eva Guillamon, Juan Luis Arques, Alfonso Gutierrez-Adan, Jose Maria Landetea
Summary: This study evaluated the health effects of two soy beverages, non-fermented and fermented, on aged female mice as a model of menopause and premenopause. The fermented soy beverage showed better outcomes in lipid profile and fertility, possibly due to the production of more bioavailable and bioactive compounds through the fermentation process.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Reproductive Biology
P. Ramos-Ibeas, A. Perez-Gomez, L. Gonzalez-Brusi, A. C. Quiroga, P. Bermejo-Alvares
Summary: Ungulate embryos undergo critical cell differentiation and proliferation events around and after blastocyst hatching. Failures in these processes lead to early pregnancy losses. Conventional embryo culture media are unable to support embryo development beyond hatching, while N2B27 medium supports early post-hatching development. The study found that culturing bovine embryos in N2B27 from day 7 onwards improves subsequent post-hatching development, providing a useful in vitro system for studying cell differentiation mechanisms and early pregnancy failures.
Article
Reproductive Biology
Osama G. Sakr, Ahmed Gad, Karina Canon-Beltran, Yulia N. Cajas, Radek Prochazka, Dimitrios Rizos, Pilar G. Rebollar
Summary: This study aimed to isolate, characterize, and identify the miRNA expression profiles in the seminal plasma extracellular vesicles (SP-EVs) isolated from fertile and subfertile rabbit bucks. The concentration of SP-EVs was positively correlated with abnormal forms and immotile spermatozoa. Small-RNA-seq analysis identified several miRNAs that were significantly up- and down-regulated in the fertile compared to the subfertile group. These findings suggest that SP-EVs with different miRNA cargo could serve as biomarkers for male fertility and potential therapies for assisted reproductive technologies.