Article
Cardiac & Cardiovascular Systems
Monil Majmundar, Kunal N. Patel, Rajkumar Doshi, Harsh Mehta, Mohinder R. Vindhyal, Kirk A. Hance, Adam Ali, Kamal Gupta
Summary: This study demonstrates that the hospital frailty risk score can identify frailty and predict clinical outcomes in patients undergoing revascularization for critical limb-threatening ischemia.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Cardiac & Cardiovascular Systems
Santeri Tarvainen, Galina Wirth, Greta Juusola, Olli Hautero, Kari Kalliokoski, Tanja Sjoros, Veikko Nikulainen, Jouni Taavitsainen, Jarkko Hytonen, Crister Frimodig, Krista Happonen, Tuomas Selander, Tomi Laitinen, Harri H. Hakovirta, Juhani Knuuti, Nihay Laham-Karam, Juha Hartikainen, Kimmo Makinen, Seppo Yla-Herttuala, Petra Korpisalo
Summary: This explorative study aimed to investigate the role of microvasculature in critical limb-threatening ischaemia (CLTI) pathology. High-resolution imaging of CLTI patients and muscle samples from amputated limbs were used to describe microvascular pathology. The findings suggest that CLTI muscles have altered blood flow dynamics and tissue damage due to hypoxia-driven capillary enlargement.
EUROPEAN HEART JOURNAL
(2023)
Review
Medicine, General & Internal
Raffaella Berchiolli, Giulia Bertagna, Daniele Adami, Francesco Canovaro, Lorenzo Torri, Nicola Troisi
Summary: This article provides an overview of the contemporary management of critical limb-threatening ischemia (CLTI) patients, with a focus on evidence-based revascularization (EBR) as a more appropriate treatment strategy. The recent BEST-CLI trial showed that autologous vein bypass graft is superior to endovascular therapy for revascularization, but there is still a critical issue for no-option CLTI patients.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Surgery
Ahmed A. Naiem, Ariane Bergeron, Kent S. MacKenzie, Daniel I. Obrand, Oren K. Steinmetz, Jason P. Bayne, Heather L. Gill, Elie Girsowicz
Summary: This study evaluated the effects of inframalleolar disease on major adverse limb events (MALE) in patients undergoing endovascular revascularization for chronic limb-threatening ischemia (CLTI). Severe IM disease was associated with lower freedom from MALE and independently increased the hazard of adverse limb outcomes and amputations in patients with CLTI by >70%. These findings highlight the importance of measuring foot perfusion in CLTI patients.
JOURNAL OF VASCULAR SURGERY
(2023)
Article
Surgery
Tony R. Soares, Goncalo Cabral, Tiago Costa, Jose Tiago, Jose Gimenez, Armanda Duarte, Diogo Cunha e Sa
Summary: This study evaluated the relationship between the global limb anatomic staging system (GLASS) stage and clinical outcomes in patients with chronic limb-threatening ischemia (CLTI) who underwent distal bypass with vein grafting. The results showed that GLASS stage was not related to patency or clinical outcomes after the surgery. Distal open revascularization resulted in excellent rates of freedom from CLTI in CLTI patients.
JOURNAL OF VASCULAR SURGERY
(2022)
Article
Surgery
Dainis Krievins, Edgars Zellans, Gustavs Latkovskis, Indulis Kumsars, Sanda Jegere, Roberts Rumba, Madara Bruvere, Christopher K. Zarins
Summary: Preoperative evaluation of CLTI patients using coronary FFRCT can reveal silent coronary ischemia, and selective postoperative coronary revascularization can reduce cardiovascular deaths and myocardial infarctions, improving survival rates.
JOURNAL OF VASCULAR SURGERY
(2021)
Article
Surgery
Aderike C. Anjorin, Christina L. Marcaccio, Priya B. Patel, Sophie X. Wang, Vincent Rowe, Bernadette Aulivola, Mark C. Wyers, Marc L. Schermerhorn
Summary: Black and Hispanic CLTI patients have worse 3-year outcomes in terms of major amputation and reintervention compared to White patients. However, Black patients have lower mortality compared to White patients, while Hispanic patients have similar mortality rates. These disparities are partially explained by differences in demographic characteristics and comorbidity prevalence.
JOURNAL OF VASCULAR SURGERY
(2022)
Article
Cardiac & Cardiovascular Systems
Ayman Elbadawi, Islam Y. Elgendy, Marwan Saad, Mohammed Elzeneini, Michael Megaly, Mohamed Omer, Subhash Banerjee, Douglas E. Drachman, Herbert D. Aronow
Summary: This study found an increase in hospitalizations for CLI among patients with diabetes in the United States. Patients who underwent revascularization had lower in-hospital mortality and major amputation rates compared to those treated medically. Endovascular revascularization was associated with lower in-hospital mortality but higher rates of major amputation compared to surgical revascularization.
JACC-CARDIOVASCULAR INTERVENTIONS
(2021)
Article
Medicine, General & Internal
Florian Simon, Mansur Duran, Waseem Garabet, Hubert Schelzig, Michael Jacobs, Alexander Gombert
Summary: A decade ago, gene therapy was seen as a potential treatment for chronic limb-threatening ischemia, but its safety and clinical feasibility are still in question. Gene therapy can be achieved through transduction and transfection methods, but there are risks involved, such as increased neo-vascularization in undesired tissue, malignant transformation and inflammation, and arterial thrombosis. Clinical trials of gene therapy in vascular occlusive disease have shown mixed results, leaving the role of gene therapy unclear.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Engineering, Biomedical
Zheng Xing, Chen Zhao, Siwen Wu, Chunchen Zhang, Haifeng Liu, Yubo Fan
Summary: Therapeutic angiogenesis provides a potential solution for critical limb ischemia by manipulating blood vessel growth in vivo without the need for extra pharmaceutics and surgery. With the rise of interdisciplinary research, regenerative medicine strategies offer new possibilities for treating various clinical diseases.
Article
Surgery
Isaac N. Naazie, Sina Zarrintan, Isibor Arhuidese, Omar Al-Nouri, Ahmed Abou-Zamzam, Mahmoud Malas
Summary: Combined suprainguinal and infrainguinal revascularization is sometimes necessary in the treatment of patients with chronic limb-threatening ischemia (CLTI). This study found that suprainguinal bypass (SIB) with concomitant infrainguinal revascularization was associated with an increased risk of amputation in patients with rest pain, whereas SIB with concomitant infrainguinal bypass was associated with a decreased risk of amputation or death in patients with tissue loss. SIB with infrainguinal revascularization should be limited in patients with rest pain in line with current guidelines, but SIB with concomitant infrainguinal bypass may be preferred in patients with tissue loss.
JOURNAL OF VASCULAR SURGERY
(2022)
Review
Cell & Tissue Engineering
Laura Lozano Navarro, Xueyi Chen, Lady Tatiana Girata Viviescas, Andrea K. Ardila-Roa, Maria L. Luna-Gonzalez, Claudia L. Sossa, Martha L. Arango-Rodriguez
Summary: Peripheral arterial disease affects hundreds of millions of people worldwide and can lead to limb amputation or death. Revascularization is crucial for preventing amputation, but a significant portion of patients are not suitable for traditional surgical treatment, resulting in higher amputation rates. Mesenchymal stem cells have emerged as a promising cell-based therapy for peripheral arterial disease due to their regenerative properties.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cardiac & Cardiovascular Systems
Ashkan Karimi, Alexis L. Lauria, Behdad Aryavand, Richard F. Neville
Summary: This review discusses the latest advancements in endovascular and surgical treatment for critical limb-threatening ischemia (CLTI). It covers novel endovascular technologies, increasing adoption of radial and pedal approaches, and surgical techniques such as distal vein patch and surgical and hybrid deep venous arterialization (DVA). Adopting these advancements in a collaborative environment can potentially reduce the rate of major amputations.
CURRENT CARDIOLOGY REPORTS
(2022)
Article
Surgery
Zhanjiang Cao, Benqi Zhao, Ti Jiang, Tong Zhang, Xiaohua Yu, Yiwen Li, Weiwei Wu
Summary: This study aimed to investigate the association between sarcopenia and long-term outcomes in patients with CLTI after endovascular revascularization. The results showed that sarcopenia was independently associated with long-term mortality, suggesting its potential as a risk factor for adverse clinical outcomes in CLTI patients.
JOURNAL OF SURGICAL RESEARCH
(2023)
Article
Medicine, General & Internal
Jayer Chung, Nikki L. B. Freeman, Michael R. Kosorok, William A. Marston, Michael S. Conte, Katharine L. McGinigle
Summary: This study aimed to evaluate the use of topic model cluster analysis to generate an accurate risk prediction model for chronic limb-threatening ischemia (CLTI). The study identified three distinct stages within CLTI and proposed a patient-centric outcome measure.
Article
Biochemistry & Molecular Biology
Lucia Beltran-Camacho, Sara Eslava-Alcon, Marta Rojas-Torres, Daniel Sanchez-Morillo, Ma Pilar Martinez-Nicolas, Victoria Martin-Bermejo, Ines Garcia de la Torre, Esther Berrocoso, Juan Antonio Moreno, Rafael Moreno-Luna, Ma Carmen Duran-Ruiz
Summary: This study evaluated the impact of serum from asymptomatic COVID-19 individuals on circulating angiogenic cells (CACs) using a proteomics-based quantitative approach. The results confirmed that SARS-CoV-2 infection in asymptomatic individuals caused protein level changes related to coagulation and inflammatory processes. Additionally, certain proteins were up-regulated only in CACs treated with serum from asymptomatic patients during the highest peak of infection. These findings provide insight into the effects of SARS-CoV-2 infection and the development of potential treatment methods.
MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Ismael Sanchez-Gomar, Josefa Benitez-Camacho, Cristina Cejudo-Bastante, Lourdes Casas, Rafael Moreno-Luna, Casimiro Mantell, Ma Carmen Duran-Ruiz
Summary: The study evaluated the potential of mango leaves, olive leaves, and red grape pomace extracts rich in polyphenols to promote ECFC reparative effects. It was found that ethanolic extracts at low concentrations improved angiogenic capacities of ECFCs and reduced proliferation, apoptosis, and the inflammatory response of these cells.
Article
Cell & Tissue Engineering
Marta Rojas-Torres, Ismael Sanchez-Gomar, Antonio Rosal-Vela, Lucia Beltran-Camacho, Sara Eslava-Alcon, Jose Angel Alonso-Pineiro, Javier Martin-Ramirez, Rafael Moreno-Luna, Ma Carmen Duran-Ruiz
Summary: Before translating ECFCs into clinical treatment, it is necessary to perform biodistribution assays to ensure biosafety and determine the distribution of cells. This study optimized a cell-tracking assay using DiR labeling and qPCR analysis, and found that intramuscularly administered ECFCs mainly remained in the ischemic tissues, while intravenously injected cells were found in multiple organs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Polymer Science
Pilar Grosso, Cristina Cejudo, Ismael Sanchez-Gomar, M. Carmen Duran-Ruiz, Rafael Moreno-Luna, Lourdes Casas, Clara Pereyra, Casimiro Mantell
Summary: The addition of natural substances with pharmacoactive properties to polymeric biomedical devices can enhance the assimilation of these implants and promote blood vessel formation. In this study, mango leaf extract (MLE) obtained through pressurized ethanol was impregnated into polylactic acid (PLA) devices. The best impregnation conditions and results were determined through in vitro culture experiments and microscopy observations.
Article
Biochemistry & Molecular Biology
Alvaro Gonzalez-Dominguez, Francisco Visiedo, Jesus Dominguez-Riscart, Ma Carmen Duran-Ruiz, Ana Saez-Benito, Alfonso M. Lechuga-Sancho, Rosa Maria Mateos
Summary: In children with obesity and insulin resistance, the activity of erythrocyte catalase is impaired due to increased S-nitrosation of the enzyme. Other post-translational modifications (PTMs) such as O-glycosylation, tyrosine nitration, and S-glutathionylation were also observed in erythrocyte catalase, but levels of O-glycosylation and S-glutathionylation were reduced in the ObIR+ group.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Medicine, Research & Experimental
Maria Calderon-Dominguez, Eva Trejo-Gutierrez, Almudena Gonzalez-Rovira, Lucia Beltran-Camacho, Marta Rojas-Torres, Sara Eslava-Alcon, Daniel Sanchez-Morillo, Juan Calderon-Dominguez, Ma Pilar Martinez-Nicolas, Estibaliz Gonzalez-Beitia, Ma Dolores Nieto-Martin, Teresa Trujillo-Soto, Manuel A. Rodriguez-Iglesias, Juan A. Moreno, Rafael Moreno-Luna, Ma Carmen Duran-Ruiz
Summary: Studies have shown that SARS-CoV-2 infection can alter the levels of microRNAs, which target key proteins involved in disease severity and progression. These findings provide potential biomarkers for COVID-19 and offer candidates for future therapeutic approaches against the disease.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Review
Pharmacology & Pharmacy
Ana Corral, Martin Alcala, Ma Carmen Duran-Ruiz, Ana I. Arroba, Jesus G. Ponce-Gonzalez, Marijana Todorcevic, Dolors Serra, Maria Calderon-Dominguez, Laura Herrero
Summary: The incidence of obesity and its related disorders has increased dramatically, and adipose tissue has been identified as a crucial regulator. Long non-coding RNAs (lncRNAs) have emerged as critical regulators of adipose tissue, and circulating lncRNAs may be valuable for the diagnosis and treatment of adipose tissue-associated disorders.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biology
Alejandra Sanchez-Brinas, Carmen Duran-Ruiz, Antonio Astola, Marta Marina Arroyo, Fatima G. Raposo, Antonio Valle, Jorge Bolivar
Summary: Research reveals that NOA36 interacts with HSPA1 and HSPA8 heat shock proteins, and plays a crucial role in cell proliferation and cell cycle.
Article
Biochemistry & Molecular Biology
Diego Valor, Antonio Montes, Maria Calderon-Dominguez, Inass Aghziel, Ismael Sanchez-Gomar, Martin Alcala, Ma Carmen Duran-Ruiz, Clara Pereyra
Summary: Submicron particles were produced from an ethanolic extract of Myrtus communnis leaves using supercritical carbon dioxide technology. The particles had small sizes, quasi-spherical shape, and high antioxidant activity. It was also observed that increasing pressure and temperature resulted in smaller particle sizes.
Article
Biochemistry & Molecular Biology
Lucia Beltran-Camacho, Santosh D. Bhosale, Daniel Sanchez-Morillo, Ismael Sanchez-Gomar, Marta Rojas-Torres, Sara Eslava-Alcon, Mario Martinez-Torija, M. Angeles Ruiz de Infante, M. Dolores Nieto-Martin, Manuel A. Rodriguez-Iglesias, Juan A. Moreno, Esther Berrocoso, Martin R. Larsen, Rafael Moreno-Luna, M. Carmen Duran-Ruiz
Summary: SARS-CoV-2 infection affects the cardiovascular system, causing vascular damage and thromboembolic events. Endothelial dysfunction is an early response to COVID-19, which may lead to cardiovascular complications and long-term sequelae. However, the molecular mechanisms involved in these processes remain poorly understood.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)